Comparison of immunodiffusion and enzyme linked immunosorbent assay for antibodies to four Aspergillus species.

Abstract:

:Antigenic extracts were prepared from Aspergillus fumigatus, A niger, A flavus and A terreus for use in enzyme linked immunosorbent assay (ELISA) and immunodiffusion (ID) tests for Aspergillus antibodies to determine whether the use of antigenic extracts from species other than A fumigatus increased the sensitivity of the ELISA. ELISA titres correlated well with positive ID tests. Patient titres by ELISA were significantly higher than control titres for all species. Patient titres to A niger were also significantly higher than titres to the other species. Total number of ID bands to A fumigatus correlated significantly with anti-A fumigatus ELISA titres. It is concluded that the use of antigenic extracts from species other than A fumigatus improves the sensitivity of the ELISA.

journal_name

J Clin Pathol

authors

Froudist JH,Harnett GB,McAleer R

doi

10.1136/jcp.42.11.1215

subject

Has Abstract

pub_date

1989-11-01 00:00:00

pages

1215-21

issue

11

eissn

0021-9746

issn

1472-4146

journal_volume

42

pub_type

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