Abstract:
:Accurate diagnosis of Clostridium difficile infection (CDI) is essential for optimal treatment, prevention and control. There are two reference assays for CDI diagnosis: the cell cytotoxicity assay (CCTA) and toxigenic culture (TC). Importantly, these tests actually detect different targets: CCTA detects the presence of C difficile toxins (primarily toxin B, but also toxin A), whereas TC detects the presence in the stool of C difficile with the potential to produce toxin. Not surprisingly studies comparing the results of these assays show imperfect agreement. Thus, a faecal sample may be CCTA negative but TC positive, and this raises the crucial question about the clinical significance of the presence of C difficile with the capacity to produce toxin but no actual detectable free toxin. A positive TC result indicates that a patient with diarrhoea is potentially infectious. TC also has the advantage that the cultured isolate is available for typing and for susceptibility testing. In general, however, CCTA has been shown to be a better test for the laboratory confirmation of CDI, although additional culture may be needed to optimise sensitivity. Crucially, when these reference assays are used to determine the accuracy of alternative diagnostic tests, care should be taken to compare methods with their appropriate standard (ie, compare tests that target equivalent end-points). Such issues have contributed to the variable and often suboptimal performance of rapid diagnostic tests for CDI. Further research is urgently needed to improve knowledge of the utility of routine diagnostic tests in CDI and the factors that influence their performance.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Planche T,Wilcox Mdoi
10.1136/jcp.2010.080135subject
Has Abstractpub_date
2011-01-01 00:00:00pages
1-5issue
1eissn
0021-9746issn
1472-4146pii
jcp.2010.080135journal_volume
64pub_type
杂志文章abstract::The epithelium of the human fallopian tube (oviduct) and cervix were studied by histological, immunohistological, and ultrastructural methods with a view to establishing the nature of the so called "basal" and "reserve" cells. The results indicated that the "basal" cells of the oviductal epithelia were T lymphocytes, ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.39.3.306
更新日期:1986-03-01 00:00:00
abstract::Electron microscopical and cytochemical studies of intestinal biopsies from a patient with typical features of the Cronkhite-Canada syndrome show that the primary process affects the crypts. This results in cystic dilatation associated with expansion and focal degeneration of the crypt compartment of the intestinal ep...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.3.271
更新日期:1985-03-01 00:00:00
abstract:AIMS:Meticillin-resistant Staphylococcus aureus (MRSA) strains isolated in Tawam Hospital, a tertiary care hospital in the United Arab Emirates, were examined in order to understand the reasons for a doubling of its incidence between 2003 and 2008 while maintaining the same infection control measures. METHODS:All cons...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2011-200436
更新日期:2012-02-01 00:00:00
abstract:BACKGROUND:Receptor activator of nuclear factor kappabeta ligand (RANKL) has an important role in bone remodelling, and tumour necrosis factor related, apoptosis inducing ligand (TRAIL) can induce apoptosis in cancer cells. Their functions are linked by their interactions with osteoprotegerin (OPG). OBJECTIVE:To inves...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.030031
更新日期:2006-07-01 00:00:00
abstract::Three hundred specimens of oesophagus obtained at necropsy from infants and children aged from 0 to 14 years, dying from a variety of causes, were examined prospectively for evidence of gastric heterotopia. Gastric heterotopia was observed in 63 (21%) of the whole series, representing a much higher incidence than repo...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.7.742
更新日期:1988-07-01 00:00:00
abstract::Enteroviruses are rare causes of acute focal encephalitis. A fatal case of echovirus type 9 infection is reported in a 9 month old boy who presented with a fever and a macular rash followed by two focal seizures. Echovirus type 9 was isolated from lung tissue after seven days. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.9.865
更新日期:1993-09-01 00:00:00
abstract::Agar gel, cellulose acetate, and starch gel electrophoresis are all capable of diagnosing sickle-cell anaemia, sickle-cell haemoglobin C disease, and haemoglobin C disease in cord blood samples. Of these three electrophoretic techniques, agar gel is the easiest to interpret.Paper electrophoresis can reliably and rapid...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.23.6.533
更新日期:1970-09-01 00:00:00
abstract::A slight modification in tissue processing and staining technique enables a previously described method for staining aluminium to be used to demonstrate aluminium in osteomalacia associated with haemodialysis. The stain appears to be accurate in diagnosing this condition and may assist in establishing the diagnosis be...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.12.1352
更新日期:1981-12-01 00:00:00
abstract::One hundred and two bone marrow samples were analysed by histological and immunohistochemical methods for neurone specific enolase (NSE). The biopsies were performed to determine the extent of bone marrow disease in 84 neuroblastomas, nine embryonal rhabdomyosarcomas, five Ewing's sarcomas, two cases of Hodgkin's dise...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.44.4.309
更新日期:1991-04-01 00:00:00
abstract:AIMS:In the gastric antrum and body, foveolar hyperplasia is a feature of reactive gastritis resulting from--for example, duodenogastric bile reflux and the use of non-steroidal anti-inflammatory drugs (NSAIDs). The aim of this study was to examine the occurrence and clinical relevance of gastric cardiac foveolar hyper...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.55.5.352
更新日期:2002-05-01 00:00:00
abstract::A study of 260 patients with non-Hodgkin's lymphoma (NHL) who underwent bilateral bone marrow biopsy at initial diagnosis showed marrow disease in 99 (38%) cases. The highest incidence of disease (83%) was seen in small lymphocytic lymphoma (SLL) and the lowest (19%) in diffuse large cell lymphoma (DLCL). Among cases ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.8.630
更新日期:1990-08-01 00:00:00
abstract::Three cases of islet cell necrosis in neonates are reported. The association of this rare condition with antepartum haemorrhage, anoxia, shock and intravascular coagulation are discussed. The clinical and morphological appearances were compared with previous descriptions of this condition and an association with neona...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.10.1206
更新日期:1987-10-01 00:00:00
abstract::Strokes are one of the most common causes of mortality and long term severe disability. There is evidence that lipoprotein (a) (Lp(a)) is a predictor of many forms of vascular disease, including premature coronary artery disease. Several studies have also evaluated the association between Lp(a) and ischaemic (thrombot...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.53.7.487
更新日期:2000-07-01 00:00:00
abstract::Two histochemical techniques, the PAT/KOH/PAS and the PBT/KOH/PAS, were used to investigate the side chain O-acyl substitution patterns of the sialic acids of the colonic epithelial mucins in cases of ulcerative colitis and Crohn's disease. In both diseases there was, as compared to normal, a reduction in the proporti...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.12.1272
更新日期:1979-12-01 00:00:00
abstract:OBJECTIVE:Few studies have modelled the economics of thyroid FNA. METHODS:A simple spreadsheet economic model for delivery of thyroid fine needle aspiration (FNA) cytology is described using the UK Royal College of Pathologists' Classification for thyroid FNA which is based on The Bethesda System for Reporting Thyroid...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2012-201339
更新日期:2013-07-01 00:00:00
abstract:BACKGROUND:Coronary heart disease is associated with increased B-type natriuretic peptides (BNPs), and, although controversial, may cause exaggerated exercise-induced BNP secretion. We investigated BNP in relation to reversible myocardial ischaemia. MATERIALS AND METHODS:Serum N-terminal proBNP (NT-proBNP) was measure...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.034702
更新日期:2006-11-01 00:00:00
abstract::IgD myeloma is a rare disorder with a very poor prognosis. An unusual case is reported where prolonged remission was obtained. Immunohistochemical staining of the bone marrow trephine biopsy specimen proved invaluable in documenting early relapse. It is suggested that immunohistochemistry should be adopted more widely...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.8.773
更新日期:1994-08-01 00:00:00
abstract:AIM:To assess the ability of the commercially available monoclonal antibody 1B12 (BioGenex, San Ramon, USA) to identify C albicans in formalin fixed, paraffin wax embedded material (FFPE). METHODS:Broth cultures of 20 strains of seven Candida species were resuspended in 4% agarose blocks, fixed in formalin for 24 hour...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.51.11.857
更新日期:1998-11-01 00:00:00
abstract:AIMS:Common variable immunodeficiency (CVID) is the most common serious primary immunodeficiency. This paper describes the immunological consequences of human immunodeficiency virus (HIV) infection in a patient with familial CVID subsequently treated with highly active antiretroviral therapy (HAART). METHODS:Serial me...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.54.9.713
更新日期:2001-09-01 00:00:00
abstract:AIMS:To investigate how the biopsy Gleason score (GS) and the clinical risk classification have been changed by the reporting rules. METHODS:565 prostate biopsy specimens were reassessed. Each Gleason pattern, 1 to 5, was interpreted according to the modified Gleason grading system proposed by the International Societ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2008.060632
更新日期:2009-03-01 00:00:00
abstract::The significance of seroconversion as detected by an ELISA screening test for hepatitis C virus (HCV) antibody with a negative supplemental/confirmatory recombinant immunoblot assay (RIBA) result was investigated. Of 118,220 established West Midlands blood donors with at least one negative HCV antibody screen, 43 had ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.49.3.254
更新日期:1996-03-01 00:00:00
abstract::Lymphoid tissue of the endometrium was analysed by histological, immunohistological, and electron microscopical methods in 10 healthy uteri. A panel of monoclonal antibodies recognising macrophages (OKMI), HLA-DR antigen, B lymphocytes, T lymphocytes and their subsets, and dendritic reticulum cells was used in a two s...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.6.644
更新日期:1985-06-01 00:00:00
abstract::A patient with multiple myeloma had antigenically related monoclonal Fc-gamma fragments and complete IgG-kappa molecules in the serum. The urine contained only Fc-gamma fragments in the absence of Bence-Jones protein. The two distinct M-components in the serum showed electrophoretic identity but could be separated by ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.33.10.936
更新日期:1980-10-01 00:00:00
abstract:AIM:To better understand the spectrum of primary lymphomas in the upper aerodigestive tract, a common site of extranodal lymphoma. MATERIALS AND METHODS:Lymphoma cases diagnosed at an institution in southern Taiwan from 1992 to 2007 were retrospectively studied with pathology and history review, immunohistochemistry, ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2010.081927
更新日期:2010-10-01 00:00:00
abstract::The sensitivity of methods to detect antibodies to intrinsic factor was assessed. Five sera of known antibody content were tested in 31 laboratories and 30 sera from patients with pernicious anaemia were tested in one laboratory. Five non-commercial methods and two kits for type I antibodies and one non-commercial met...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.2.210
更新日期:1989-02-01 00:00:00
abstract:AIMS:Venous invasion (VI) is a known independent prognostic indicator of recurrence and survival in colorectal cancer. The guidelines of the Royal College of Pathologists (RCPath) state that, in a series of resections, extramural VI should be detected in at least 25% of specimens. However, there is widespread variabili...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2011-200156
更新日期:2011-11-01 00:00:00
abstract:AIM:The prognostic significance of perineural invasion (PNI) in oesophageal adenocarcinoma (EAC) is unclear. We examined the association of PNI with clinical outcomes in patients treated with neoadjuvant chemoradiotherapy (nCRT) and surgery. METHODS:We performed a single institutional retrospective study. We evaluated...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2020-206424
更新日期:2020-04-21 00:00:00
abstract::Acute myocardial disease, which may be associated with abnormal platelet activity, has been reported after routine immunisation. Thirty-two army apprentices undergoing immunisation were studied for changes in the heparin neutralising activity (HNA) of platelet poor plasma. HNA increased after immunisation. This increa...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.11.1152
更新日期:1979-11-01 00:00:00
abstract:AIMS:To assess the association between Helicobacter pylori-associated gastritis and HLA-DR antigen (class II antigen) expression. METHODS:Fifty endoscopic gastric biopsy specimens were studied for the presence of H pylori, degree and type of inflammation, and for HLA-DR antigen expression in the epithelium. The cases ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.45.1.30
更新日期:1992-01-01 00:00:00
abstract:INTRODUCTION:The study sought to determine whether mast cell counts in endobronchial biopsies of large airways are related to mast cell counts in the small airways. METHODS:Lungs, obtained postmortem from 10 subjects who had died of non-respiratory causes, were fixed in inflation. Mast cell densities (cells/mm(3)), de...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2010.079574
更新日期:2011-05-01 00:00:00