Abstract:
AIM:To assess the ability of the commercially available monoclonal antibody 1B12 (BioGenex, San Ramon, USA) to identify C albicans in formalin fixed, paraffin wax embedded material (FFPE). METHODS:Broth cultures of 20 strains of seven Candida species were resuspended in 4% agarose blocks, fixed in formalin for 24 hours, and embedded in paraffin wax. In addition, 16 blocks of FFPE tissue known to contain periodic acid-Schiff positive fungal hyphae were examined. Antigen retrieval involved microwave treatment of specimens in citrate buffer (0.01 M; pH 6.5) before addition of 1B12 antibody for 24 hours. Bound antibody was subsequently detected using a biotinylated link antibody and a peroxidase conjugated streptavidin. RESULTS:Only C albicans strains were 1B12 positive in the agarose blocks. All FFPE tissue blocks were found to contain 1B12 positive hyphal structures, indicating the presence of C albicans. CONCLUSIONS:The ability to identify candida organisms penetrating the lesional tissue in cases of chronic hyperplastic candidosis will help to clarify the role of individual Candida spp in this important form of oral candidosis.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Williams DW,Jones HS,Allison RT,Potts AJ,Lewis MAdoi
10.1136/jcp.51.11.857keywords:
subject
Has Abstractpub_date
1998-11-01 00:00:00pages
857-9issue
11eissn
0021-9746issn
1472-4146journal_volume
51pub_type
杂志文章abstract::The relationship between the intensity of the delayed hypersensitivity reaction and the dose of antigen used for its elicitation change with time after sensitization. At three weeks the reactions to 100 mug of egg albumen were little different from those to 1 mug but at 14 weeks 100 mug gave much stronger reactions. T...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.26.9.687
更新日期:1973-09-01 00:00:00
abstract::Human papillomavirus antigen was found in 39 (16%) of 253 colposcopic biopsies from a group of women at high risk for cervical cancer who had been examined in the early 1970s. Immunohistochemical evidence of papillomavirus infection was found in 20 (30%) of 67 cervical intraepithelial neoplasia lesions infected with w...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.8.855
更新日期:1985-08-01 00:00:00
abstract:BACKGROUND:The disorders associated with metabolic syndrome (MS) can lead to renal disease. IgA nephropathy is the most common form of glomerulonephritis, and many patients with this disorder progress to renal failure. AIMS:To identify the effect of MS on IgA nephropathy by retrospectively comparing patients who had I...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2009.074278
更新日期:2010-08-01 00:00:00
abstract::To determine the functional importance of the low B12 and red cell folate concentrations repeatedly observed in the elderly 200 consecutive patients admitted to a geriatric unit were studied. Forty six of the patients had low serum concentrations of B12 (15), red cell folate (26), or both (five). Serum B12 and red cel...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.10.1179
更新日期:1985-10-01 00:00:00
abstract:AIMS:To determine the sensitivity and specificity of a multiplex PCR assay for the contemporary identification of major species involved in oral candidiasis, without extraction and purification of DNA from the samples under investigation; and evaluation of this method in comparison with routine phenotypic culture ident...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2006.040782
更新日期:2007-09-01 00:00:00
abstract::The aim of this article is to provide knowledge of the origin of catecholamines and metabolites so that there can be an informed approach to the methods for biochemical screening for a possible phaeochromocytoma; The article includes a review of catecholamine and metadrenaline metabolism, with methods used in biochemi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2009.071647
更新日期:2010-08-01 00:00:00
abstract::An anaerobic incubator was compared with a standard jar system for the isolation of anaerobes from clinical material. Seventy specimens were selected as likely to yield anaerobes: 342 different anaerobes were isolated in the incubator and 347 in anaebrobic jars. These included Bacteroides spp (43%), Peptococcus spp (2...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.35.10.1158
更新日期:1982-10-01 00:00:00
abstract::The production of antitoxin after Clostridium difficile-induced diarrhoea has not been reported previously. The stool of a patient with prolonged antibiotic-associated diarrhoea contained C. difficile toxin, and the serum neutralised the cytopathic effect of C. difficile toxin in tissue culture. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.4.414
更新日期:1981-04-01 00:00:00
abstract::Haemoglobin (Hb) Bonn is a newly described benign Hb variant that causes falsely depressed oxygen saturation as measured by pulse oximetry. It was found to be associated with mild haemolysis. Since its first report in a German family, no further cases have been documented in the literature. We report the first Chinese...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2010.078147
更新日期:2010-10-01 00:00:00
abstract:AIMS:The aim of this study was to evaluate the mismatch repair gene expression and their correlation with NF-kB in patients with oral squamous cell carcinoma (SCC). METHODS:Total RNA was isolated from 28 biopsy samples. A control group was composed of 20 volunteers. Differential expression of hMSH2, hMSH6 and NF-kB ge...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2019-206161
更新日期:2020-05-01 00:00:00
abstract::Electron microscopy od duodenal mucosa from children with acute non-bacterial enteritis has shown virus particles in epithelial cells. Indirect immunofluorescent techniques applied to the same tissue showed virus antigen localized in the cytoplasm of epithelial cells of the villi. Specific IgM antibody was present in ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.28.4.263
更新日期:1975-04-01 00:00:00
abstract:AIMS:To develop a strategy for neonatal screening of sickle cell disease (SCD) and effective enrollment of affected neonates in a comprehensive follow-up programme adapted to the socioeconomic conditions, health structures and cultural background of an African setting. METHODS:The strategy implemented at the two large...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,多中心研究
doi:10.1136/jcp.2008.059113
更新日期:2009-01-01 00:00:00
abstract::Two diabetic patients with symptomatic urinary tract infection by Torulopsis glabrata were successfully treated with two-week courses of 5-fluorocytosine. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.27.1.50
更新日期:1974-01-01 00:00:00
abstract:AIMS:Common variable immunodeficiency (CVID) is the most common serious primary immunodeficiency. This paper describes the immunological consequences of human immunodeficiency virus (HIV) infection in a patient with familial CVID subsequently treated with highly active antiretroviral therapy (HAART). METHODS:Serial me...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.54.9.713
更新日期:2001-09-01 00:00:00
abstract::Three cases of plasmacytoma of the thyroid are described and their clinical and histological features recorded. Two were considered to be true extramedullary plasmacytomas, the third the initial manifestation of a disseminated myelomatous process. Plasmacytomas of the thyroid are shown to have many features in common ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.21.5.661
更新日期:1968-09-01 00:00:00
abstract:OBJECTIVES:As the best characterised cancer/testis antigen family members, melanoma-associated antigens (MAGE) have been reported to be expressed in various malignant tumours. However, the expression pattern of MAGE-A family in lung adenocarcinoma (LAC) specimens and their prognostic and therapeutic significance for pa...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2016-203718
更新日期:2017-06-01 00:00:00
abstract::In two out of three cases of idiopathic fibrosis of the mediastinum there was also retroperitoneal fibrosis, and in one of them extensive fibrosis of the lungs. One of these, a fit middle-aged man, died suddenly with severe coronary artery stenosis due to fibrosis. The third case was that of a young man with a solitar...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.1.78
更新日期:1978-01-01 00:00:00
abstract::The incidence of malignant melanoma has increased steadily over the past 30 years and this type of malignancy is the leading cause of death from cutaneous malignant disease. Cutaneous malignancies, including melanoma, can be detected at a very early stage and a cure is possible with prompt detection and treatment. In ...
journal_title:Journal of clinical pathology
pub_type: 指南,杂志文章,实务指引
doi:10.1136/jcp.53.8.587
更新日期:2000-08-01 00:00:00
abstract::A family with hypobetalipoproteinaemia was studied to examine the Lp(a) lipoprotein, lipoprotein cholesterol, and triglyceride composition of the serum lipids. Lp(a) lipoprotein was measured by immunoassay. Serum lipoproteins were separated by ultracentrifugation. Cholesterol and triglycerides were measured using stan...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.6.587
更新日期:1995-06-01 00:00:00
abstract:AIMS:Caucasian patients with disorders of sex development (DSD) are at a high risk of developing germ cell cancer (GCC). GCC is prominent in young adults in Western countries, while the incidence is significantly lower in Asia. So far, the risk of GCC in Asian DSD patients is unknown. METHODS AND RESULTS:A detailed st...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2012-201062
更新日期:2013-03-01 00:00:00
abstract::A unique case of a metastatic non-desmoplastic sarcomatoid malignant melanoma in an axillary lymph node showing smooth muscle differentiation in a 54 year old woman is described. The tumour cells exhibited alpha-smooth muscle actin, HHF-35 and desmin positivity but were negative for S100 protein and HMB-45. Ultrastruc...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.49.11.950
更新日期:1996-11-01 00:00:00
abstract::Serious infection caused by Lancefield group C streptococci is unusual in man. Two unrelated deaths associated with these organisms in a 55 year old woman who died after three days of diarrhoea and vomiting, and in a 65 old man who died after a week of non-specific symptoms, are presented. Post mortem examination show...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.10.965
更新日期:1993-10-01 00:00:00
abstract::A method of typing isolates of Proteus mirabilis and Proteus vulgaris is described based on the sensitivity of the organisms to bacteriocine. Twelve standard proteocine producing strains were selected from a large number of isolates tested, and from these liquid proteocine preparations were prepared. The sensitivities...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.28.10.784
更新日期:1975-10-01 00:00:00
abstract::Aneuploidy is frequently noted in malignant tumours. There is much controversy about its cause and effect in relation to malignant tumours. Failure of the spindle checkpoint caused by mutation of the responsible genes may be one of the important factors for the development of aneuploidy. Telomere dysfunction may also ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2004.018952
更新日期:2004-12-01 00:00:00
abstract::Toxoplasmosis is an infection, congenital or acquired, caused by Toxoplasma gondii. A pilot study using toxoplasmin skin antigen was undertaken to establish the incidence of the infection in the London area as a preliminary to a larger study of the effects of the infection upon pregnancy, the foetus, and the newborn i...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.22.6.649
更新日期:1969-11-01 00:00:00
abstract::Of the 123 patients with acute mesenteric infarction treated over the past 12 years, 16 (13%) had mesenteric venous thrombosis. Eight of the patients with mesenteric venous thrombosis survived the initial episode; two have since died. The remaining six patients were studied for evidence of haemostatic deficiencies or ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.40.8.906
更新日期:1987-08-01 00:00:00
abstract:BACKGROUND:Gastric mucosa associated lymphoid tissue (MALT) lymphoma is a low grade B cell lymphoma histologically characterised by neoplastic B cells surrounding follicles in a marginal zone pattern and selectively infiltrating epithelium to form characteristic lymphoepithelial lesions. AIMS:To identify solitary epit...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2004.023747
更新日期:2005-11-01 00:00:00
abstract::The sensitivity patterns to a number of antibiotic-impregnated discs of 126 strains of Gram-negative anaerobes and 36 strains of Gram-positive non-sporing anaerobes from various sources have been examined. The sensitivity patterns shown by Bacteroids fragilis and Bacteroides melaninogenicus were useful for the rapid i...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.28.5.388
更新日期:1975-05-01 00:00:00
abstract:BACKGROUND:The Glasgow Prognostic Score (GPS), an inflammation-based prognostic score formed from standard thresholds of C reactive protein (CRP) and albumin, has prognostic value in patients with advanced cancer. Little is known about the general biochemical disturbance associated with the systemic inflammatory respon...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,多中心研究
doi:10.1136/jcp.2005.033217
更新日期:2007-06-01 00:00:00
abstract::The activities of phosphohexose isomerase and 6-phosphogluconate dehydrogenase have been studied in the vaginal fluid of women from the gynaecological departments. Women from these departments gave a very high false positive rate, though no cases of carcinoma in situ were not detected by estimating phosphohexose isome...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.19.4.378
更新日期:1966-07-01 00:00:00