Abstract:
AIMS:To determine the sensitivity and specificity of a multiplex PCR assay for the contemporary identification of major species involved in oral candidiasis, without extraction and purification of DNA from the samples under investigation; and evaluation of this method in comparison with routine phenotypic culture identification. METHODS:78 oral rinse solutions were collected. The concentrated oral rinse technique was used for a quantitative and qualitative study. Research and identification of Candida spp, with routine phenotypic culture identification (germ-tube test in serum at 37 degrees C for 3 hours and sugar assimilation strip analysis), were performed. Each sample was analysed with multiplex PCR directly on oral rinse solution. Samples giving discrepant results between routine phenotypic and PCR identification methods were resubcultured on CHROMagar Candida plates. The fungus-specific primers ITS1, ITS2, CA3, and CA4 were used. For the identification of other species (C kefyr, C famata and C dubliniensis), ITS1F, ITS1K, and ITS2D primers were designed. RESULTS:Multiplex PCR correctly identified all samples, including those with single species, or with mixed species, negative samples and positive samples which appeared to be negative from routine phenotypic methods. CONCLUSION:This multiplex PCR assay provides a rapid alternative to the conventional culture based technique for the identification and speciation of the most frequently isolated Candida species. The absence of an extraction method made identification of 10 species possible in a few hours.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Liguori G,Lucariello A,Colella G,De Luca A,Marinelli Pdoi
10.1136/jcp.2006.040782subject
Has Abstractpub_date
2007-09-01 00:00:00pages
1035-9issue
9eissn
0021-9746issn
1472-4146pii
60/9/1035journal_volume
60pub_type
杂志文章abstract::A small epidemic of gastroenteritis in a children's hospital is described, and also the biochemical characteristics of the causative organism. Stress is laid on the fact that failure to recognize that epidemic strains of Escherichia coli may be late lactose fermentors can lead to errors or delay in diagnosis. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.15.5.482
更新日期:1962-09-01 00:00:00
abstract:AIMS:To institute recommendations from a laboratory turnround time study; to evaluate audit methods; and to quantify improvements achieved. METHODS:Changes to result report distribution and specimen delivery were affected by posting results directly from the laboratory followed by the introduction of a twice daily cou...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.4.372
更新日期:1995-04-01 00:00:00
abstract::Although the morphology of the pathognomonic Reed-Sternberg cells of Hodgkin lymphoma (HL) was described over a century ago, it was not until recently that their origin from B lymphocytes was recognised. The demonstration that a proportion of cases of HL harbour the Epstein-Barr virus (EBV) and that its genome is mono...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2007.050146
更新日期:2007-12-01 00:00:00
abstract:AIMS:To evaluate an antigen capture enzyme linked immunosorbent assay (ELISA) which detects a carbohydrate epitope on the excretory-secretory (ES) antigens of Toxocara canis in clinical practice. METHODS:Serum specimens from healthy adults, patients with acute visceral larva migrans, ocular and inactive toxocariasis, ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.6.551
更新日期:1993-06-01 00:00:00
abstract::Cutaneous melanoma causes most of the skin cancer deaths in the USA. Melanoma is the fifth most common cancer among men and the sixth most common cancer among women. The incidence of melanoma has risen sharply over the past three decades. In this review, which is informed by our extensive experience at a large cancer ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2018-205417
更新日期:2019-04-01 00:00:00
abstract::The routine estimation of large numbers of serum folic acid and vitamin B(12) levels using the Baird and Tatlock(1) Analmatic clinical system is described and the method evaluated.Precision, accuracy, and carryover are shown to be at acceptable levels, and the advantages and disadvantages of the use of the system are ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.24.1.18
更新日期:1971-02-01 00:00:00
abstract::Two cases of anticonvulsant megaloblastic anaemia are described, showing features of unusual interest. Though both cases were apparently deficient in folic acid, the Figlu tests were negative. One patient had an extremely low serum B(12) concentration apparently associated with defective B(12) absorption due to defici...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.18.5.593
更新日期:1965-09-01 00:00:00
abstract::The necropsy findings in an elderly woman, who had been treated with methyldopa and who had died during treatment for accidental hypothermia, showed acute periductal inflammation of obviously similar duration in an entopic pancreas in the wall of the jejunum and in the ectopic pancreas. This supports the hypothesis th...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.41.4.430
更新日期:1988-04-01 00:00:00
abstract::The costs of a clinical chemistry laboratory in a district general hospital were studied. The system used has certain advantages over the conventional Cooper Lybrand method. The time taken by technicians to perform tests was more variable than expected and the cost of sample collection was higher than process-cost for...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.6.589
更新日期:1981-06-01 00:00:00
abstract::Haemoglobin, haematocrit, and plasma iron values were estimated on blood samples collected from 100 male and 113 female National Blood Transfusion Service blood donors, and from 83 male and 66 female age-matched control subjects. No significant differences in any of these values were found to exist between the donors ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.22.6.680
更新日期:1969-11-01 00:00:00
abstract:AIMS:The aim of this study was to assess the prevalence and drug resistance of Ureaplasma species and Mycoplasma hominis in outpatients with genital manifestation from 2005 to 2013 in Hangzhou, China. METHODS:A total of 2689 female and 2336 male patients with various genital symptoms were included in this study. Speci...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202248
更新日期:2014-09-01 00:00:00
abstract::Two diabetic patients with symptomatic urinary tract infection by Torulopsis glabrata were successfully treated with two-week courses of 5-fluorocytosine. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.27.1.50
更新日期:1974-01-01 00:00:00
abstract::The principal neuropathological and general pathological findings in a group of 24 patients with renal transplants who died in a nine-year period at the Western Infirmary, Glasgow, are described. Opportunistic infections--bacterial, protozoal, and fungal--were the commonest causes of death. Other causes included cardi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.35.1.31
更新日期:1982-01-01 00:00:00
abstract::The Kallestad Corporation recently suggested that their new buffer system for the nephelometric detection of rheumatoid factor conferred advantages over existing systems. Two rate nephelometric procedures, the Kallestad QM-300 and the Beckman ICS-II (RF), were therefore compared. Sera (n = 157) were selected on the ba...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.3.243
更新日期:1990-03-01 00:00:00
abstract::Breast fibroepithelial lesions, including fibroadenomas and phyllodes tumours, are commonly encountered in clinical practice. As histological differences between these two related entities may be subtle, resulting in a challenging differential diagnosis, pathological techniques to assist the differential diagnosis of ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2015-203231
更新日期:2015-12-01 00:00:00
abstract:AIMS:The probabilistic approach is widely adopted for breast fine needle aspiration cytology. However, a definite cytological diagnosis is not always possible for C3 (atypia) cases, which poses a management dilemma as this represents a mixed category of benign and malignant cases. It would be beneficial to be able to p...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,多中心研究
doi:10.1136/jclinpath-2016-204138
更新日期:2017-12-01 00:00:00
abstract::Lymphocyte-depleted classical Hodgkin lymphoma (LDCHL) is the rarest entity among the classical Hodgkin lymphoma (CHL) subtypes, accounting for <1% of CHL cases in Western countries. Similarly, primary splenic Hodgkin lymphoma is a rare diagnosis among all cases of lymphomatous involvement of spleen. Representing a co...
journal_title:Journal of clinical pathology
pub_type: 信件
doi:10.1136/jclinpath-2015-203145
更新日期:2015-11-01 00:00:00
abstract:AIMS:To compare the expression of immunohistochemical variables between benign and malignant components of malignant peripheral nerve sheath tumour (MPNST) arising within neurofibroma. METHODS:Eight cases of MPNST arising within a neurofibroma, associated with neurofibromatosis type 1 (NF1), were studied. The areas of...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.54.8.631
更新日期:2001-08-01 00:00:00
abstract::This paper illustrates a simple method of necropsy dissection of the abdominal organs after abdominal surgery. The organs are removed in one block as per the method described by Letulle. A retroperitoneal approach is then used. Structures are dissected away in a series of layers using the vasculature for guidance. Thi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.49.9.776
更新日期:1996-09-01 00:00:00
abstract:AIM:To ascertain the views of Scottish hospital laboratory users on aspects of regional and supraregional biochemical services offered by the Institute of Biochemistry at Glasgow Royal Infirmary. METHODS:A questionnaire was circulated asking questions or inviting opinions under various headings, including current patt...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.47.5.395
更新日期:1994-05-01 00:00:00
abstract::Recent publications have suggested that fixing neutrophils in formalin is a useful adjunct to the differentiation of antinuclear antibodies from perinuclear antineutrophil cytoplasmic antibodies. In this small comparative study of 30 sera the use of an additional slide of formalin fixed neutrophils did not add any use...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.48.1.89
更新日期:1995-01-01 00:00:00
abstract::As part of a quality assurance programme at the Aga Khan University Medical Centre, Karachi, Pakistan, all hysterectomy specimens were reviewed from January 1986 to December 1989. Adenomyosis was found in 237 of the 419 (56.5%) specimens studied. Of these 237 patients, 232 (97.9%) were parous and 196 (82.8%) were in t...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.10.817
更新日期:1990-10-01 00:00:00
abstract:BACKGROUND:Gastric mucosa associated lymphoid tissue (MALT) lymphoma is a low grade B cell lymphoma histologically characterised by neoplastic B cells surrounding follicles in a marginal zone pattern and selectively infiltrating epithelium to form characteristic lymphoepithelial lesions. AIMS:To identify solitary epit...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2004.023747
更新日期:2005-11-01 00:00:00
abstract:AIMS:To study whether differences in eradication rates of antibiotics may be explained by differences in secretion of antibiotics in gastric juice. METHODS:A single intravenous dose of either ampicillin 500 mg, erythromycin 500 mg, or metronidazole 500 mg was administered to four healthy Helicobacter pylori negative v...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.45.3.225
更新日期:1992-03-01 00:00:00
abstract::A new monoclonal antibody, Ber-EP4, directed against a partially formol resistant epitope on the protein moiety of two 34 kilodalton and 39 kilodalton glycopolypeptides on human epithelial cells is described. Immunostaining of a wide range of normal and neoplastic human tissues and cell lines showed that all carcinoma...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.43.3.213
更新日期:1990-03-01 00:00:00
abstract::Antithrombin activities in 30 severely malnourished children and 40 normal children were estimated in clotting tests by thrombin neutralisation as anti-Xa and by a heparin antithrombin assay; and by immunodiffusion as alpha 2-globulin and alpha 1-antitrypsin. The patients' mean alpha 2-globulin was severely depressed,...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.10.1025
更新日期:1979-10-01 00:00:00
abstract:AIMS:To show that medullary carcinomas of the thyroid are morphologically indistinguishable from gut carcinoids: the value of histochemistry in their identification and differential diagnosis from metastatic carcinoid tumours to the thyroid and some follicular cell neoplasms. METHODS:15 thyroid medullary carcinomas wi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.2.113
更新日期:1993-02-01 00:00:00
abstract::As part of a study to establish the prevalence of renal calculus disease, alkaline phosphatase activity was measured by the method recommended by the Scandinavian Society for Clinical Chemistry and Clinical Physiology in the plasma of 3823 adults from the Scottish new town of Cumbernauld. The enzyme activity differs i...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.12.1202
更新日期:1978-12-01 00:00:00
abstract::An immunochemical procedure for routine laboratory diagnosis of pregnancy utilizing adsorption of human chorionic gonadotrophin onto kaolin and the Ortho pregnancy test reagents is described. The method is suitable for quantitative assay of urinary human chorionic gonadotrophin and can be adapted for use with serum. I...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.17.5.548
更新日期:1964-09-01 00:00:00
abstract:AIMS:It has been suggested that adenocarcinomas of the lower oesophagus and gastric cardia should be reclassified as oesophagogastric junction (OGJ) cancers. This study aimed to define the frequency of OGJ cancers in a geographically defined population of 4.3 million people. METHODS:All cases of oesophageal and gastri...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.55.3.191
更新日期:2002-03-01 00:00:00