Abstract:
:Neurons from cerebral neocortex and hippocampus exhibit a striking difference in vulnerability to transient global ischemia. In order to study the contribution of neuronal connections and neuron-glia interactions to this variation in neuronal vulnerability, we used hippocampal and neocortical cultures submitted to various periods of histotoxic ischemia. Organotypic cultures were exposed at 37 degrees C for 0, 7, 30 and 60 min to a glucose-free NaCN-containing medium. Histological analysis using thionin staining and MAP2 immunostaining showed differences in the temporal profile of neuronal damage in hippocampal and neocortical structures, i.e., in decreasing order, CA1 (7 min) > CA3 and neocortical layers II, III, V, VI (30 min) > DG and neocortical layer IV (60 min). In parallel to the neurodegeneration study, the time course and the regional pattern of microglial and astroglial changes were also examined using GS-B4 isolectin and GFAP as immunohistochemical markers, respectively. The GS-B4 isolectin staining revealed an early (at 7 min for the hippocampus) and a specific microglial activation located in areas undergoing neuronal damage. For both organotypic cultures, astrogliosis occurred later (after 30 min of stress) with no specific regional distribution. Both hippocampal and neocortical cultures submitted to histotoxic ischemia allowed the replication of many of the cellular events observed in response to global ischemia in vivo. These findings support the hypothesis that neuron-neuron connections as well as interactions between neurons and glial cells are essential to reproduce in vitro the selective neuronal vulnerability described in vivo.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Bernaudin M,Nouvelot A,MacKenzie ET,Petit Edoi
10.1006/exnr.1997.6728subject
Has Abstractpub_date
1998-03-01 00:00:00pages
30-9issue
1eissn
0014-4886issn
1090-2430pii
S0014-4886(97)96728-5journal_volume
150pub_type
杂志文章abstract::Spinal motor neurons of Sprague-Dawley rats were examined to determine which of the neuronal isoforms of actin (beta or gamma) upregulate following axon injury. In situ hybridization studies showed greater beta-actin mRNA levels but no change in gamma-actin mRNA levels-suggesting that axon regrowth utilizes beta-actin...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.2002.8034
更新日期:2002-12-01 00:00:00
abstract::Neuronal apoptosis is a central pathological process in subarachnoid hemorrhage (SAH)-induced early brain injury. Previous studies indicated that ErbB4 (EGFR family member v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4) is essential for normal development and maintenance of the nervous system. In this...
journal_title:Experimental neurology
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更新日期:2017-11-01 00:00:00
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journal_title:Experimental neurology
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更新日期:1987-02-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(87)90261-5
更新日期:1987-12-01 00:00:00
abstract::In order to study the ocular dominance and responsiveness of cells in the deafferented visual cortex, the geniculate and the callosal inputs were interrupted in adult cats by either simultaneous (OTCCX) or separate surgical transection of the optic tract (OTX) and the posterior corpus callosum (CCX). Unit recording wa...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(88)90174-4
更新日期:1988-03-01 00:00:00
abstract::The contribution of chondroitin sulfate proteoglycan (CSPG) in the suppression of axonal growth in rat spinal cord has been examined by means of an in vitro bioassay in which regenerating neurons are grown on tissue section substrata. Dissociated embryonic chick dorsal root ganglionic neurons were grown on normal and ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1998.6951
更新日期:1998-12-01 00:00:00
abstract::Poor survival of embryonic dopamine neurons is a primary problem limiting the value of neurotransplantation for Parkinson's disease. Several neurotrophic factors have been shown to promote dopamine neuron survival when used individually in culture. We have found that two peptides, insulin-like growth factor-I (IGF-I) ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1996.0115
更新日期:1996-07-01 00:00:00
abstract::We investigated the involvement of central nervous system nicotinic-acetylcholine receptors in the secretory activity of the hypothalamic-hypophyseal-adrenal axis. Antiacetylcholine receptor antibodies derived from rabbits immunized with purified Torpedo-acetylcholine receptor or from myasthenia gravis patients were i...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(86)90251-7
更新日期:1986-12-01 00:00:00
abstract::The ability of striatal embryonic grafts to promote functional recovery on complex behavioral tasks depends on various factors, including the amount of striatal-like tissue within the grafts and the duration of post-graft training. However, how the innate paw bias of animals is affected by experience, or influences re...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2005.12.033
更新日期:2006-06-01 00:00:00
abstract::Prostaglandin and thromboxane levels increase in mammalian brain during seizures, but whether a metabolite of arachidonic acid has a role in induction, or is merely a by-product, of seizures is still not clear. It has been shown that nonsteroidal anti-inflammatory drugs block arachidonic acid metabolism through inhibi...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(87)90080-x
更新日期:1987-10-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(89)90131-3
更新日期:1989-09-01 00:00:00
abstract::We used [2-14C]deoxyglucose as a marker of increased metabolism of the hypoglossal nucleus after transection of its nerve. We studied this metabolism in 3-, 12-, and 24-month-old rats. We found an increase in glucose uptake in the control nucleus of 24-month-old rats which was significant when compared to that of 3-mo...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(90)90012-h
更新日期:1990-04-01 00:00:00
abstract::For most individuals, the respiratory control system produces a remarkably stable and coordinated motor output-recognizable as a breath-from birth until death. Very little is understood regarding the processes by which the respiratory control system maintains network stability in the presence of changing physiological...
journal_title:Experimental neurology
pub_type: 杂志文章,评审
doi:10.1016/j.expneurol.2016.07.012
更新日期:2017-01-01 00:00:00
abstract::Neuronal migration was investigated in rodent cerebellum in vitro and in vivo. Time-lapse video recording showed that cultured neurons migrated on laminin by first extending neurites that formed contacts with other neurons. This was followed by movement of the cell nucleus inside the preformed process. No guidance cue...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(92)90119-b
更新日期:1992-08-01 00:00:00
abstract::An in vitro study of the nuclear magnetic resonance (NMR) response of protons in normal and pathological human skeletal muscle is presented. Muscle biopsies from 34 patients with various neuromuscular diseases and five normal subjects were studied by proton NMR techniques and the results were compared with those of li...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(83)90159-0
更新日期:1983-07-01 00:00:00
abstract::Genetic and protein studies have indicated abnormalities in alpha-synuclein in neurodegenerative diseases. However, the developmental localization and cellular role of synuclein isoforms is contentious. We investigated the cellular localization of alpha-, beta-, and gamma-synuclein in developing cultured rat neurons a...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/s0014-4886(03)00108-0
更新日期:2003-07-01 00:00:00
abstract:BACKGROUND:Novel strategies to improve hand function after stroke are needed. Electromyography-triggered functional neuromuscular stimulation (EMG-FNMS) and repetitive transcranial magnetic stimulation (rTMS) are promising techniques to facilitate recovery of sensory-motor hand dysfunction after stroke. OBJECTIVE:To i...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2011.04.010
更新日期:2011-07-01 00:00:00
abstract::This article reviews stem cell-based strategies for spinal cord injury repair, and practical issues concerning their translation to the clinic. Recent progress in the stem cell field includes clinically compliant culture conditions and directed differentiation of both embryonic stem cells and somatic stem cells. We pr...
journal_title:Experimental neurology
pub_type: 杂志文章,评审
doi:10.1016/j.expneurol.2007.09.002
更新日期:2008-02-01 00:00:00
abstract::The role of central noradrenergic neurons in kindled seizures was assessed by comparison of alpha 2- and beta-adrenoceptor binding in the cerebral cortex from kindled and control rats. To minimize handling, which may modify kindling-induced changes in binding, the kindling protocol involved stimulation of the amygdala...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(85)90044-5
更新日期:1985-10-01 00:00:00
abstract::The long-term effects of partial denervation on the muscle fiber cross-sectional area, degree of axonal sprouting, and end-plate morphology were examined 1, 3, 6, 9, and 12 months postsurgery in rat plantaris muscle. After 1 month of partial denervation, mean cross-sectional areas of type I and type II myofibers were ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(92)90004-a
更新日期:1992-06-01 00:00:00
abstract::Neuronal activity in the locus ceruleus of awake, behaving stump-tailed monkeys increased consistently and vigorously after presentation of mildly aversive and threatening stimuli. These preliminary data increase the range of stimuli reported to elicit locus ceruleus activity in awake monkeys to include nonpainful thr...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(84)90217-6
更新日期:1984-06-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2006.08.002
更新日期:2007-01-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(85)90087-1
更新日期:1985-06-01 00:00:00
abstract::Indirect epidemiological and experimental evidence suggest that the severity of injury during stroke is influenced by prior sleep history. The aim of our study was to test the effect of acute sleep deprivation on early outcome following experimental stroke. Young male Sprague-Dawley rats (n=20) were subjected to focal...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2009.12.023
更新日期:2010-03-01 00:00:00
abstract::Previous studies have suggested that defective high-affinity glutamate uptake, due mainly to a major loss of the astroglial-specific GLT-1 glutamate transporter, underlies the selective motoneuron degeneration observed in sporadic ALS (24, 28). If a defect in glutamate transport underlies the pathogenesis of sporadic ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1996.0198
更新日期:1996-12-01 00:00:00
abstract::An autoradiographic method is described for detecting changes in ornithine decarboxylase in axotomized superior cervical ganglion neurons of rats using [3H]difluoromethylornithine. An increase in binding to neurons was seen at 12 h and 1 day after crushing the postganglionic nerves. Binding returned to control values ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(86)90097-x
更新日期:1986-05-01 00:00:00
abstract::A central role for noradrenergic dysregulation in the pathophysiology of post-traumatic stress disorder (PTSD) is increasingly suggested by both clinical and basic neuroscience research. Here, we integrate recent findings from clinical and animal research with the earlier literature. We first review the evidence for n...
journal_title:Experimental neurology
pub_type: 杂志文章,评审
doi:10.1016/j.expneurol.2016.05.014
更新日期:2016-10-01 00:00:00
abstract::Functional regeneration of brainstem-spinal pathways occurs in the developing chick when the spinal cord is severed prior to embryonic day (E) 13. Functional spinal cord regeneration is not observed in animals injured after E13. This developmental transition from a permissive to a restrictive repair period may be due ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.2000.7496
更新日期:2000-11-01 00:00:00
abstract::In the intraluminal suture model of middle cerebral artery occlusion (MCAO) in the rat, lesions of the masticator muscles associated with impaired functional outcome occur. We evaluated the role of external carotid artery (ECA) transection. We assessed whether isolated interruption of an arterial or a venous connectio...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2005.05.013
更新日期:2005-10-01 00:00:00
abstract::Heme oxygenase-1 is a cellular stress protein expressed in brain and other tissues in response to oxidative challenge and other noxious stimuli. In the present study, immunohistochemistry was used to assess HO-1 expression in various postmortem human brain specimens derived from PD and control subjects. In the substan...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1997.6752
更新日期:1998-03-01 00:00:00