Abstract：
BACKGROUND & AIMS:Peroxynitrite (PN), a potent oxidant, has been implicated in the pathogenesis of gut inflammation and epithelial cell apoptosis. The aim of this study was to investigate mesalamine, a standard therapy for inflammatory bowel disease, to see if it attenuates PN-induced cytotoxicity in human intestinal epithelial cells and if mesalamine directly interacts with PN or its precursor, nitric oxide. METHODS:T84 and HT29 cells were divided in several protocols: mesalamine was administered 2 hours before, simultaneously, or 30 minutes after PN. T84 cells, grown in filter chamber inserts, were used to determine if basolateral or apical administration of PN initiated apoptosis and epithelial barrier function. The effects of mesalamine on PN decomposition and NO half-life were determined. RESULTS:Mesalamine resulted in a dose-dependent decrease in PN-induced apoptosis, whether mesalamine was administered before (>10 micromol/L; IC50, 16 micromol/L), simultaneously (25-200 micromol/L; IC50, 24 micromol/L), or 30 minutes (200 micromol/L) after PN exposure. Mesalamine protected the epithelial barrier function of T84 cells against PN. Mesalamine rapidly degraded PN, whereas the half-life of NO was not affected. CONCLUSIONS:The beneficial effects of mesalamine in the treatment of inflammatory bowel disease may involve an attenuation of PN-induced cell injury and apoptosis through direct and indirect mechanisms without affecting NO levels.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Sandoval M,Liu X,Mannick EE,Clark DA,Miller MJdoi
10.1053/gast.1997.v113.pm9352850subject
Has Abstractpub_date
1997-11-01 00:00:00pages
1480-8issue
5eissn
0016-5085issn
1528-0012pii
S0016508597005143journal_volume
113pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract：BACKGROUND & AIMS:Notch downstream targets are fundamental to intestinal cell lineage commitment and are suggested as therapeutic targets for colon cancer cells. However, the role of endogenous Notch signaling through receptor-ligand interaction, and effects of its longer term down-regulation on intestinal homeostasis,...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2008.05.050
更新日期：2008-09-01 00:00:00
abstract：BACKGROUND & AIMS:The mechanism by which sulindac causes regression of adenomas in patients with familial adenomatous polyposis (FAP) is unclear. Conflicting data on the drug's effects on colorectal epithelial proliferation have been reported. An alternative mechanism, and one not previously studied, is via induction o...
journal_title：Gastroenterology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/0016-5085(95)90411-5
更新日期：1995-09-01 00:00:00
abstract：BACKGROUND & AIMS:The physiologic function of the efflux transporter Multidrug Resistance Protein 3 (MRP3) remains poorly defined. In vitro, MRP3 transports several glucuronidated compounds, but the compounds transported under physiologic conditions are unknown. Knowledge of the compounds transported by MRP3 in vivo wo...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2009.06.052
更新日期：2009-11-01 00:00:00
abstract：BACKGROUND & AIMS:Corticosteroids have been shown to significantly decrease short-term mortality in patients with severe alcoholic hepatitis. However, independent factors associated with a favorable outcome and long-term survival are unknown. The aim of this study was to examine prognostic factors and long-term surviva...
journal_title：Gastroenterology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1053/gast.1996.v110.pm8964410
更新日期：1996-06-01 00:00:00
abstract：:Twenty cirrhotic patients with esophageal varices and an episode of gastrointestinal bleeding within the previous week underwent hemodynamic studies which used an indicator dilution technique based on selective catheterization of the splanchnic arteries and hepatic vein. Portasystemic shunting of splenic arterial flow...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1976-06-01 00:00:00
abstract：BACKGROUND & AIMS:In patients with chronic Helicobacter pylori (H pylori) infection, parietal and chief cell atrophy in the gastric corpus, a process known as spasmolytic polypeptide-expressing metaplasia (SPEM), increases the risk for progression to cancer. The relation between H pylori and these metaplastic changes i...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2018.09.050
更新日期：2019-01-01 00:00:00
abstract：:Ileocecal sphincter pressure was studied in the cat, using an open-tipped perfused catheter system. Ileocecal sphincter pressure was measured under basal conditions and in response to bethanechol or direct vagal nerve electrical stimulation. The pressure was 26.1 +/- 5.0 mmHg basally, and was unaltered by section of e...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1981-05-01 00:00:00
abstract：BACKGROUND & AIMS:Rotavirus infection is a leading cause of morbidity and mortality in children younger than 5 years of age. Current treatment options are limited. We assessed the efficacy of a llama-derived, heavy-chain antibody fragment called anti-rotavirus protein (ARP1), in modifying the severity and duration of d...
journal_title：Gastroenterology
pub_type： 杂志文章,随机对照试验
doi：10.1053/j.gastro.2013.06.053
更新日期：2013-10-01 00:00:00
abstract：BACKGROUND & AIMS:The cytokine macrophage migration inhibitory factor (MIF) is expressed throughout the human gastrointestinal tract. Recently, protumorigenic activity of MIF has been described in several cancer models. Therefore, we investigated the expression and function of MIF during the early stages of intestinal ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2005.07.061
更新日期：2005-11-01 00:00:00
abstract：BACKGROUND/AIMS:Helicobacter felis colonizes the gastric mucosa of rodents. Preliminary studies showed differences in the distribution of the organism in different parts of the stomach that seemed related to the secretory capacity of the mucosa. The aim of this study was to determine the localization of H. felis in the...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(95)90686-x
更新日期：1995-05-01 00:00:00
abstract：:Several single-nucleotide polymorphisms (SNPs) have been associated with colorectal cancer (CRC) susceptibility. Most CRCs arise from adenomas, and SNPs therefore might affect predisposition to CRC by increasing adenoma risk. We found that 8 of 18 known CRC-associated SNPs (rs10936599, rs6983267, rs10795668, rs3802842...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2012.09.016
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND/AIMS:The yeast Saccharomyces boulardii inhibits the secretion induced by cholera toxin (CT) in rat jejunum. The present study was aimed at unraveling the mechanism by which S. boulardii protects intestinal cells against CT. METHODS:CT-induced adenosine 3',5'-cyclic monophosphate (cAMP) levels were measured ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/s0016-5085(94)94403-2
更新日期：1994-01-01 00:00:00
abstract：:The kinetics of cholesterol and bile acid was determined from a 10-week analysis of the biliary lipids after a single injection of labeled cholesterol in parallel with a conventional 1-week study of primary bile acid kinetics. Six healthy controls and 6 patients with cholesterol cholelithiasis were studied. Two of the...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1975-08-01 00:00:00
abstract：BACKGROUND & AIMS:Patients with sphincter of Oddi dysfunction are at high risk of developing pancreatitis after endoscopic biliary sphincterotomy. Impaired pancreatic drainage caused by pancreatic sphincter hypertension is the likely explanation for this increased risk. A prospective, randomized controlled trial was co...
journal_title：Gastroenterology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0016-5085(98)70031-9
更新日期：1998-12-01 00:00:00
abstract：BACKGROUND & AIMS:Methylation of specific microRNAs (miRNAs) often occurs in an age-dependent manner, as a field defect in some instances, and may be an early event in colitis-associated carcinogenesis. We aimed to determine whether specific mRNA signature patterns (MIR1, MIR9, MIR124, MIR137, MIR34B/C) could be used t...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2017.08.037
更新日期：2017-12-01 00:00:00
abstract：:There is considerable evidence that fluids are removed from the peritoneal cavity by drainage into lymphatics lining the surface of the diaphragm, but there is little quantitative information on the rate of reabsorption as affected by conditions which exist in cirrhotic ascites. In the present study a plethysmographic...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1977-11-01 00:00:00
abstract：BACKGROUND/AIMS:Little is known about the evolution of morphological changes in pelvic ileal-pouch mucosa. This study evaluates prospectively the sequence of early morphological, histochemical, and phenotypic features in pouch mucosal biopsy specimens. METHODS:Twenty-two patients with pelvic ileal pouches constructed ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(94)90169-4
更新日期：1994-08-01 00:00:00
abstract：:A patient with ulcerative ileojejunitis was studied by determination of HL-A phenotype, by measurement of jejunal IgA synthesis using a labeled amino acid incorporation technique, and by in vitro organ culture. The patient carried the HL-A8 phenotype in common with 87.5% of patients with gluten-sensitive enteropathy. ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1975-03-01 00:00:00
abstract：BACKGROUND & AIMS:As life expectancy increases, there are greater numbers of patients with liver diseases who require surgery or transplantation. Livers of older patients have significantly less reparative capacity following ischemia and reperfusion (I/R) injury, which occurs during these operations. There are no strat...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2011.08.005
更新日期：2011-12-01 00:00:00
abstract：:Pancreatic ductal adenocarcinoma is a devastating disease, and patient outcomes have not improved in decades. Treatments that target tumor cells have largely failed. This could be because research has focused on cancer cells and the influence of the stroma on tumor progression has been largely ignored. The focus of pa...
journal_title：Gastroenterology
pub_type： 杂志文章,评审
doi：10.1053/j.gastro.2012.11.037
更新日期：2013-06-01 00:00:00
abstract：:We report a patient with severe peptic ulcer disease and a right ovarian mass that was found to be a gastrin-producing cystadenocarcinoma. Gastrin production by the tumor was stimulated by secretin and inhibited by the long-acting somatostatin analogue SMS 201-995. Following resection of the tumor, serum gastrin level...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(89)90084-x
更新日期：1989-08-01 00:00:00
abstract：:Celiac sprue and Crohn's disease have very rarely been documented in the same patient. We report a case involving an adolescent male with known Crohn's disease who had extreme wasting, growth retardation, intractable diarrhea, and delay in development of secondary sexual characteristics. Peroral jejunal biopsy showed ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：
更新日期：1977-04-01 00:00:00
abstract：BACKGROUND & AIMS:Corticotropin-releasing factor (CRF) signaling pathways play a key role in the stress response through the activation of CRF(1) and CRF(2) receptors. We investigated the CRF receptor subtypes involved in gastric postoperative ileus. METHODS:Adult male mice (C57BL/6, CRF(1)-deficient, and wild-type), ...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/s0016-5085(03)01069-2
更新日期：2003-09-01 00:00:00
abstract：BACKGROUND & AIMS:Gastroenteropancreatic neuroendocrine cells secrete chemical messengers in a calcium-dependent fashion. So far, other second messenger systems involved in regulated secretion have gained little attention. The aim of this study was to characterize guanosine 3',5'-cyclic monophosphate (cGMP)-mediated ve...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/s0016-5085(98)70593-1
更新日期：1998-04-01 00:00:00
abstract：:Canine jejunal epithelial cells were isolated and maintained in short-term culture to study cholecystokinin (CCK) release. Sequential digestion of jejunal mucosa with collagenase and ethylenediaminetetraacetic acid was followed by counterflow elutriation to enrich CCK-containing cells. After 40 hours in culture on col...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(92)91780-8
更新日期：1992-01-01 00:00:00
abstract：BACKGROUND & AIMS:Helicobacter pylori infection is mainly acquired in early childhood, but the exact routes of transmission remain elusive. To distinguish between risks of intrafamilial and extraneous child-to-child transmission, we studied H. pylori seroprevalence among Swedish school children with varying family back...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/gast.2001.26282
更新日期：2001-08-01 00:00:00
abstract：BACKGROUND & AIMS:Estimates of complication rates following elective percutaneous liver biopsy vary and might not accurately reflect current practice. We studied mortality and complication rates, by indication, in patients after they underwent liver biopsies. METHODS:We performed a study using hospital episode statist...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2010.06.015
更新日期：2010-10-01 00:00:00
abstract：BACKGROUND & AIMS:Surgical management of pancreatic cancer depends on tumor resectability and staging. This study evaluated a new in vivo technique, fiberoptic confocal fluorescence microscopy (FCFM), for detection and staging of pancreatic tumors in rats. METHODS:FCFM was used with a protease-activated fluorescent ma...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2009.07.045
更新日期：2009-11-01 00:00:00
abstract：:A case of spontaneous rupture of a nonparasitic liver cyst, documented by serial computed tomography scans, is reported. The patient was closely followed clinically after the rupture of the cyst and was treated conservatively, without surgical intervention. No peritonitis developed. This unusual complication of nonpar...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1016/0016-5085(89)91438-8
更新日期：1989-07-01 00:00:00
abstract：BACKGROUND & AIMS:Biomarkers are needed to risk stratify after chemoradiotherapy for localized esophageal cancer. These could improve identification of patients at risk for cancer progression and selection of additional therapy. METHODS:We performed deep sequencing (CAncer Personalized Profiling by deep Sequencing, [C...
journal_title：Gastroenterology
pub_type： 杂志文章
doi：10.1053/j.gastro.2019.10.039
更新日期：2020-02-01 00:00:00
