Abstract:
BACKGROUND & AIMS:In patients with chronic Helicobacter pylori (H pylori) infection, parietal and chief cell atrophy in the gastric corpus, a process known as spasmolytic polypeptide-expressing metaplasia (SPEM), increases the risk for progression to cancer. The relation between H pylori and these metaplastic changes is unclear. We investigated whether H pylori localizes to regions of SPEM. METHODS:We developed an in situ adherence assay in which we incubated H pylori with free-floating tissue sections from the gastric corpora of mice; we assessed H pylori distribution along the gastric unit by immunofluorescence. We analyzed the interactions of H pylori with tissue collected from mice with acute SPEM, induced by high-dose tamoxifen. We also evaluated how adhesin-deficient H pylori strains, chemical competition assays, and epithelial glycosylation affected H pylori adhesion to SPEM glands. Mice colonized with the mouse-adapted PMSS1 strain were analyzed for H pylori colonization in vivo during tamoxifen-induced SPEM or after decrease of stomach acid with omeprazole. RESULTS:Compared with uninjured glands, H pylori penetrated deep within SPEM glands, in situ, through interaction of its adhesin, SabA, with sialyl-Lewis X, which expanded in SPEM. H pylori markedly increased gastric corpus colonization when SPEM was induced, but this proximal spread reversed in mice allowed to recover from SPEM. Decreasing corpus acidity also promoted proximal spread. However, H pylori penetrated deep within corpus glands in vivo only when sialyl-Lewis X expanded during SPEM. CONCLUSIONS:Helicobacter pylori differentially binds SPEM glands in situ and in mice, in large part by interacting with sialyl-Lewis X. Our findings indicate that H pylori expands its niche into the gastric corpus by promoting and exploiting epithelial metaplastic changes that can lead to tumorigenesis.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Sáenz JB,Vargas N,Mills JCdoi
10.1053/j.gastro.2018.09.050subject
Has Abstractpub_date
2019-01-01 00:00:00pages
160-174.e7issue
1eissn
0016-5085issn
1528-0012pii
S0016-5085(18)35081-9journal_volume
156pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract::Gallbladder stasis has been implicated in gallstone formation. Gallbladder filling and emptying were quantitated by computer-assisted cholescintigraphy in 41 normal subjects versus 26 patients with gallstones. Gallbladder contraction was induced by low-dose (1.2 U/kg . h) cholecystokinin infusion. Gallstone patients e...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(85)90152-0
更新日期:1985-03-01 00:00:00
abstract::The purpose of the study was to determine (a) the frequency and cause of mortality in patients with chronic pancreatitis; (b) the cumulative survival rates corrected by comparison of patients with a matched French population; and (c) the factors associated with mortality by a unidimensional and multidimensional analys...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1989-04-01 00:00:00
abstract::Ornithine decarboxylase activity is known to be increased in certain premalignant conditions. We determined the activity of this enzyme in mucosal biopsy specimens from 15 patients with Barrett's esophagus. Ornithine decarboxylase was greater in Barrett's mucosa than in squamous esophageal or gastric mucosa. In Barret...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(88)90259-4
更新日期:1988-03-01 00:00:00
abstract:BACKGROUND & AIMS:We have shown that addition of gum arabic (GA) to a 90 mmol/L sodium-111 mmol/L glucose oral rehydration solution (ORS) enhances its effectiveness for water and electrolyte absorption in normal rats. The present study extends these observations on GA in ORS to two rat models of diarrheal disease. MET...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.1997.v112.pm9178690
更新日期:1997-06-01 00:00:00
abstract:BACKGROUND & AIMS:Pancreatic ductal adenocarcinoma (PDAC) is a highly invasive and metastatic disease for which conventional treatments are of limited efficacy. A number of agents in development are potential anti-invasive and antimetastatic agents, including the Src kinase inhibitor dasatinib. The aim of this study wa...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2010.03.034
更新日期:2010-07-01 00:00:00
abstract:BACKGROUND & AIMS:The vagus nerve negatively regulates macrophage cytokine production via the release of acetylcholine (ACh) and activation of nicotinic acetylcholine receptors (nAChR). In various models of intestinal inflammation, vagus nerve efferent stimulation ameliorates disease. Given the actively constrained cyt...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2009.04.057
更新日期:2009-09-01 00:00:00
abstract:BACKGROUND & AIMS:Senescence prevents cellular transformation. We investigated whether vascular endothelial growth factor (VEGF) signaling via its receptor, VEGFR2, regulates senescence and proliferation of tumor cells in mice with colitis-associated cancer (CAC). METHODS:CAC was induced in VEGFR2(ΔIEC) mice, which do...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2015.03.016
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND & AIMS:De novo hepatitis B virus (HBV)-related hepatitis after chemotherapy results in high morbidity and mortality. We evaluate the clinical course of de novo HBV-related hepatitis after chemotherapy. METHODS:Two hundred forty-four consecutive hepatitis B surface antigen (HBsAg)-negative lymphoma patients ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2006.04.015
更新日期:2006-07-01 00:00:00
abstract:BACKGROUND & AIMS:People infected with human immunodeficiency virus (HIV) have an increased risk of some malignancies, but little is known about the effects of infection on risk of cancers of the upper gastrointestinal tract. We evaluated the risks of different histologic and anatomic subtypes of carcinomas and non-Hod...
journal_title:Gastroenterology
pub_type: 杂志文章,收录出版
doi:10.1053/j.gastro.2012.07.013
更新日期:2012-10-01 00:00:00
abstract::Lamins have important roles in nuclear structure and cell signaling. Several diseases are associated with mutations in the lamin A/C gene (LMNA in humans). Patients with familial partial lipodystrophy caused by LMNA mutations develop pancreatitis, but lamin function in the pancreas and how these mutations affect pancr...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2018.01.024
更新日期:2018-05-01 00:00:00
abstract::Women are underrepresented in gastroenterology. A questionnaire examined the training and career experiences of female members of the Canadian Association for Gastroenterology (CAG) and their male counterparts. A pretested questionnaire was mailed to the 50 female CAG and 100 age-matched male members. Descriptive anal...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.1997.v113.pm9247490
更新日期:1997-08-01 00:00:00
abstract::Intracerebroventricular prostaglandin E2 (PGE2) inhibits stimulated gastric acid secretion; however, the central site of action is unknown. Specific PGE2 binding sites have been localized to the ventromedial hypothalamic nucleus and central amygdala (A). The nuclear accumbens has been shown to play a role in central n...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(91)90198-t
更新日期:1991-02-01 00:00:00
abstract::To determine whether pancreatic polypeptide (PP) is a physiologic inhibitor of pancreatic secretion, we have studied the effects of normal rabbit serum and anti-PP serum on pancreatic secretion during infusion of a physiologic dose of PP or intragastric food instillation in dogs. Infusion of 180 pmol/kg X h of PP, pre...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(86)90263-5
更新日期:1986-06-01 00:00:00
abstract::We analyzed the clinical, radiographic, esophageal manometric, and pathologic features of 26 women with severe, idiopathic constipation. Twenty-four patients were between 19 and 39 yr of age. Stool frequency was once every 5-28 days. On barium enema examination, 9 of 24 patients had colons of increased length and 4 of...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(85)80128-1
更新日期:1985-01-01 00:00:00
abstract::To find a possible explanation for the selective hepatic conjugation of bile acids with glycine or taurine, the N-acyl amidates of cholic acid and a number of amino acids and amino acid analogues were synthesized, and their susceptibility to hydrolysis by pancreatic juice, gastric juice, serum, or small intestinal muc...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(86)90925-x
更新日期:1986-02-01 00:00:00
abstract::A 14-yr-old girl presented with long-standing symptoms of partial gastric outlet obstruction due to an antral mucosal diaphragm that had a central aperture of 4-5 mm. The literature of this congenital malformation is reviewed with respect to diagnostic criteria and feasibility of endoscopic transection. The procedure ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(85)90222-7
更新日期:1985-11-01 00:00:00
abstract::Spinal cord stimulation is an increasingly popular form of pain treatment. An electrode positioned on the dorsal aspect of the spinal cord at the level of the nerve roots from the painful area stimulates the spinal cord. Current from the electrode is supplied by a pulse generator in the lower anterior abdominal wall. ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(99)70570-6
更新日期:1999-07-01 00:00:00
abstract::Behavioral economic approaches could increase uptake for colorectal cancer screening. We performed a randomized controlled trial of 2245 employees to determine whether an email containing a phone number for scheduling (control), an email with the active choice to opt in or opt out (active choice), or the active choice...
journal_title:Gastroenterology
pub_type: 杂志文章,随机对照试验
doi:10.1053/j.gastro.2017.07.015
更新日期:2017-11-01 00:00:00
abstract:BACKGROUND & AIMS:The effect of endogenous hypergastrinemia on growth of human colon carcinoma is not known. Our aim was to study the growth of human colon carcinoma in an animal model with endogenous hypergastrinemia. METHODS:Human colon carcinoma was transplanted to the colon of 40 athymic rats. Of these, 25 underwe...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(95)90625-8
更新日期:1995-11-01 00:00:00
abstract:BACKGROUND & AIMS:Prophylactic administration of interleukin (IL)-10 decreases the severity of experimental pancreatitis. Prevention of post-endoscopic retrograde cholangiopancreatography (ERCP) pancreatitis in humans is a unique model to study the potential role of IL-10 in this setting. METHODS:In a single-center, d...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1053/gast.2001.21172
更新日期:2001-02-01 00:00:00
abstract:BACKGROUND & AIMS:Development of pancreatic ductal adenocarcinoma (PDA) involves acinar to ductal metaplasia and genesis of tuft cells. It has been a challenge to study these rare cells because of the lack of animal models. We investigated the role of tuft cells in pancreatic tumorigenesis. METHODS:We performed studie...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2020.07.037
更新日期:2020-11-01 00:00:00
abstract:BACKGROUND & AIMS:Intrapancreatic activation of digestive enzymes is a key event in the parenchymal cell injury of pancreatitis. We hypothesized that neutrophils recruited to the pancreas during pancreatitis may contribute to such activation. METHODS:To cause experimental pancreatitis, rats and mice were treated with ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.2002.32409
更新日期:2002-04-01 00:00:00
abstract:BACKGROUND & AIMS:Microsatellite instability is a property of most tumors occurring in the context of hereditary nonpolyposis colon cancer. Instability also occurs in 10%-15% of apparently sporadic colorectal cancers, and it has been hypothesized that this instability may indicate a genetic predisposition to colonic ca...
journal_title:Gastroenterology
pub_type: 评论,杂志文章
doi:10.1016/0016-5085(95)90742-4
更新日期:1995-12-01 00:00:00
abstract::Decreases in arterial pH markedly increase sodium, chloride, and water absorption in the normal ileum and can reverse ongoing cholera toxin-induced secretion. In the current study we examined whether these effects of pH are evident in other models of ileal secretion, and in a model of increased absorption. Rats were a...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(89)91556-4
更新日期:1989-02-01 00:00:00
abstract::Regional specific antibodies and chromatography were used to analyze the distributions and molecular forms of peptide histidine isoleucine (PHI) and vasoactive intestinal peptide (VIP) in the porcine intestine. Both peptides were present along the entire length of the intestine, the highest concentrations occurring in...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(85)90445-7
更新日期:1985-09-01 00:00:00
abstract::We are delighted to introduce a new and simple format in the references section of the articles we publish in Gastroenterology that highlights joint first authors of citations. We believe this is an important step to acknowledging equal contribution to published work as research becomes increasingly team-oriented and ...
journal_title:Gastroenterology
pub_type: 社论
doi:10.1053/j.gastro.2012.08.009
更新日期:2012-10-01 00:00:00
abstract:BACKGROUND & AIMS:Apart from its importance as an acid secretogogue, the role of histamine as a downstream target of gastrin has not been fully explored. Previous studies have shown that the combination of hypergastrinemia and Helicobacter infection resulted in accelerated gastric cancer in mice. We used this model to ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2005.03.027
更新日期:2005-06-01 00:00:00
abstract:BACKGROUND & AIMS:Peroxynitrite (PN), a potent oxidant, has been implicated in the pathogenesis of gut inflammation and epithelial cell apoptosis. The aim of this study was to investigate mesalamine, a standard therapy for inflammatory bowel disease, to see if it attenuates PN-induced cytotoxicity in human intestinal e...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.1997.v113.pm9352850
更新日期:1997-11-01 00:00:00
abstract::Focal nodular hyperplasia (FNH) and hepatocellular adenoma (HCA) are benign hepatocellular tumors that develop most frequently in women without cirrhosis. Genomic approaches have identified signaling pathways related to these benign hepatocyte proliferations. FNH, a polyclonal lesion, is characterized by local vascula...
journal_title:Gastroenterology
pub_type: 杂志文章,评审
doi:10.1053/j.gastro.2013.02.032
更新日期:2013-05-01 00:00:00
abstract:BACKGROUND & AIMS:A Th1 clone, II-6, established from an autoimmune gastritis BALB/c mouse that underwent thymectomy 3 days after birth, recognized a 15 mer peptide constructing the alpha subunit of H+, K(+)-adenosine triphosphatase as antigen and induced gastritis in nu/nu mice by adoptive transfer. The aim of this st...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(96)70063-x
更新日期:1996-10-01 00:00:00