In vitro autoradiographic localization of 5-HT1A receptor-activated G-proteins in the rat brain.


:Serotonin 5-HT1A receptors belong to the superfamily of G-protein-coupled receptors. Receptor activation of G-proteins can be determined by agonist-stimulated [35S]GTPgammaS binding in the presence of excess GDP, and in vitro autoradiographic adaptation of this technique allows visualization of receptor-activated G-proteins in tissue sections. The present study was performed to examine 5-HT1A receptor activation of G-proteins using 8-OH-DPAT-stimulated [35S]GTPgammaS binding in membranes and brain sections. In hippocampal membranes, 8-OH-DPAT stimulated [35S]GTPgammaS binding by twofold, with an ED50 value of 25 nM. 5-HT1 antagonists, but not 5-HT2 antagonists, increased the ED50 of 8-OH-DPAT in a manner consistent with competitive antagonists. Scatchard analysis of [35S]GTPgammaS binding showed that 8-OH-DPAT induced the formation of high affinity [35S]GTPgammaS binding sites with a KD for GTPgammaS of 3.2 nM. [35S]GTPgammaS autoradiography, performed in brain sections with the 5-HT1A agonist 8-OH-DPAT, revealed high levels of 5-HT1A-stimulated [35S]GTPgammaS binding in the hippocampus, lateral septum, prelimbic cortex, entorhinal cortex, and dorsal raphe nucleus. 5-HT1A-stimulated [35S]GTPgammaS binding in sections was blocked by the addition of the 5-HT1 antagonist methiothepin. These results show that the use of agonist-stimulated [35S]GTPgammaS autoradiography for the 5-HT1A receptor system should provide new information regarding signal transduction in specific brain regions.


Brain Res Bull


Brain research bulletin


Sim LJ,Xiao R,Childers SR




Has Abstract


1997-01-01 00:00:00














  • The effect of hypothalamic temperature on the immune response in the rat.

    abstract::To clarify the role of febrile temperatures on the immune system, rats were immunized with sheep erythrocytes and their core temperature was then changed by continuously cooling or heating the preoptic area for five days. Core temperatures of up to 2 degrees C above or below normal were associated with a high titre of...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Brandt S,Banet M

    更新日期:1984-08-01 00:00:00

  • Golgi impregnated somatostatin immunoreactive neurons in lamina II of the rat spinal cord.

    abstract::The morphology of somatostatin immunoreactive (SOM-I) neurons in lamina (L) II of the rat spinal cord was determined using a combination of Golgi impregnation and immunohistochemistry. Golgi-impregnated SOM-I neurons that resembled islet, stalked and other cells were observed. Islet cells are considered to be inhibito...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Mather CS,Ho RH

    更新日期:1992-02-01 00:00:00

  • Cerebrospinal fluid and plasma concentrations of oxytocin and vasopressin during parturition and vaginocervical stimulation in the sheep.

    abstract::Simultaneous blood and cerebrospinal fluid (CSF) samples were taken from conscious sheep before, during and after parturition. Concentrations of plasma and CSF oxytocin were significantly elevated during contractions and particularly at birth. Mean prepartum CSF concentrations of oxytocin were around 55% of those foun...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Kendrick KM,Keverne EB,Hinton MR,Goode JA

    更新日期:1991-05-01 00:00:00

  • The conduction velocity and central projections of retinofugal fibers in the rabbit.

    abstract::IN anesthetized and paralyzed rabbits, action potentials were elicited antidromically following electrical stimulation of optic tract terminals at the geniculate level (LGN). The conduction velocity spectrum extended from 7 m.s-1 to 34 m.s-1. The distribution of conduction velocities indicated four major modes at 10, ...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Molotchnikoff S,Lessard I

    更新日期:1980-11-01 00:00:00

  • Projecting neurons in spinal dorsal horn send collateral projections to dorsal midline/intralaminar thalamic complex and parabrachial nucleus.

    abstract::Itch is an annoying sensation that always triggers scratching behavior, yet little is known about its transmission pathway in the central nervous system. Parabrachial nucleus (PBN), an essential transmission nucleus in the brainstem, has been proved to be the first relay station in itch sensation. Meanwhile, dorsal mi...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Li JN,Ren JH,Zhao LJ,Wu XM,Li H,Dong YL,Li YQ

    更新日期:2021-01-25 00:00:00

  • Neuroprotective effect of transretinal electrical stimulation on neurons in the inner nuclear layer of the degenerated retina.

    abstract::Electrical stimulation has been shown to have neuroprotective effects on ganglion cells and photoreceptors in axotomized and dystrophic retinas from Royal College of Surgeons (RCS) rats. This study determined whether electrical stimulation also has a neuroprotective effect on cells in the inner nuclear layer (INL) of ...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Schmid H,Herrmann T,Kohler K,Stett A

    更新日期:2009-04-06 00:00:00

  • Glucose-sensitive neurons of the globus pallidus: I. Neurochemical characteristics.

    abstract::The lateral hypothalamic area (LHA) and globus pallidus (GP) are basically involved in the regulation of feeding and metabolic processes. In the LHA, glucose-sensitive (GS) neurons were described: their activity was found to be specifically suppressed by electrophoretic application of glucose, and these neurons appear...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Lénárd L,Karádi Z,Faludi B,Czurkó A,Niedetzky C,Vida I,Nishino H

    更新日期:1995-01-01 00:00:00

  • Localization of androgen receptor within peptidergic neurons of the rat forebrain.

    abstract::This study tested for the presence of androgen receptor-immunoreactivity in somatostatin, galanin, vasopressin, corticotropin-releasing hormone, and oxytocin neurons in the rat forebrain. The brains of adult male Sprague-Dawley rats were fixed with 4% paraformaldehyde. Androgen receptor was visualized in coronal secti...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Bingaman EW,Baeckman LM,Yracheta JM,Handa RJ,Gray TS

    更新日期:1994-01-01 00:00:00

  • Role of iNOS-NO-cGMP signaling in modulation of inflammatory and myelination processes.

    abstract::Nitric oxide (NO) is the main activator of the soluble guanylate cyclase (sGC)-guanosine 3'5' cyclic monophosphate (cGMP) pathway. The level of cGMP is regulated by phosphodiesterases (PDEs), which break down cGMP. It has been reported that levels of NO in the central nervous system (CNS) can greatly increase during d...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Rapôso C,Luna RL,Nunes AK,Thomé R,Peixoto CA

    更新日期:2014-05-01 00:00:00

  • Detailed mapping of CGRP mRNA expression in the rat central nervous system: comparison with previous immunocytochemical findings.

    abstract::The localization of CGRP mRNA in neurons of the rat brain and spinal cord was assessed by in situ hybridization histochemistry (ISH) using a radiolabeled synthetic 57-mer oligodeoxynucleotide probe complementary to the rat prepro CGRP mRNA. Results were compared with previously published findings of CGRP-immunoreactiv...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Kresse A,Jacobowitz DM,Skofitsch G

    更新日期:1995-01-01 00:00:00

  • Extracellular signal-regulated kinases mediate melittin-induced hypersensitivity of spinal neurons to chemical and thermal but not mechanical stimuli.

    abstract::Subcutaneous melittin injection causes central plasticity at the spinal level in wide-dynamic-range (WDR) neurons, which are hypersensitive to various nociceptive stimuli. Previous behavioral studies demonstrated that the mitogen-activated protein kinases (MAPKs) extracellular signal-regulated kinase 1/2(ERK1/2), p38 ...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Li MM,Yu YQ,Fu H,Xie F,Xu LX,Chen J

    更新日期:2008-11-25 00:00:00

  • Involvement of the serotonergic system in the hypoactive and antinociceptive effects of nicotine in mice.

    abstract::Pretreatment with the 5-HT2 antagonist ketanserin and the 5-HT1A/2 antagonist spiperone did not reduce nicotine-induced hypomotility in mice, nor did MDL 7222, a selective 5-HT3 antagonist. In addition, 8-OH-DPAT and buspirone, 5-HT1A agonists, had no significant effects on nicotine-induced hypomotility. However, 8-OH...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Damaj MI,Glennon RA,Martin BR

    更新日期:1994-01-01 00:00:00

  • Minocycline treatment prevents depression and anxiety-like behaviors and promotes neuroprotection after experimental ischemic stroke.

    abstract::Depression and anxiety have been reported as the major neuropsychiatric consequences following stroke. Minocycline, a neuroprotective drug has minimized depressive symptoms in patients with major depressive disorders and anxiety-like symptoms. In addition, minocycline demonstrated efficacy and seemed a promising neuro...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Camargos QM,Silva BC,Silva DG,Toscano ECB,Oliveira BDS,Bellozi PMQ,Jardim BLO,Vieira ÉLM,de Oliveira ACP,Sousa LP,Teixeira AL,de Miranda AS,Rachid MA

    更新日期:2020-02-01 00:00:00

  • Physical exercise improves motor and short-term social memory deficits in reserpinized rats.

    abstract::Previous studies have shown that cognitive deficits precede the classical motor symptoms seen in Parkinson's disease (PD) and that physical exercise may exert beneficial effects on PD. We have recently verified that the monoamine-depleting drug reserpine - at doses that do not modify motor function - impairs memory pr...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Aguiar AS Jr,Araújo AL,da-Cunha TR,Speck AE,Ignácio ZM,De-Mello N,Prediger RD

    更新日期:2009-08-14 00:00:00

  • Brain beta-endorphin-like immunoreactivity in adult rats given beta-endorphin neonatally.

    abstract::beta-endorphin-like immunoreactivity was measured by radioimmunoassay in the brains of adult rats treated neonatally with beta-endorphin, naloxone, or vehicle. After treatment with beta-endorphin, the decreases observed in beta-endorphin-like immunoreactivity in the hypothalamus, pineal, midbrain, pons-medulla, hippoc...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Moldow RL,Kastin AJ,Hollander CS,Coy DH,Sandman CA

    更新日期:1981-12-01 00:00:00

  • Is fipronil safer than chlorpyrifos? Comparative developmental neurotoxicity modeled in PC12 cells.

    abstract::Fipronil, a GABA(A) receptor antagonist, is replacing many insecticide uses formerly fulfilled by organophosphates like chlorpyrifos. Few studies have addressed the potential for fipronil to produce developmental neurotoxicity. We compared the neurotoxicity of fipronil and chlorpyrifos in undifferentiated and differen...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Lassiter TL,MacKillop EA,Ryde IT,Seidler FJ,Slotkin TA

    更新日期:2009-03-30 00:00:00

  • The medullary dorsal reticular nucleus facilitates acute nociception in the rat.

    abstract::The influence on pain processing caused by destruction or stimulation of the dorsal reticular nucleus (DRt) was studied using the tail-flick and the increasing temperature hot-plate tests. Lesions of the DRt were obtained by injecting quinolinic acid (180 nmol/microliters) unilaterally or bilaterally, and nociceptive ...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Almeida A,Tjølsen A,Lima D,Coimbra A,Hole K

    更新日期:1996-01-01 00:00:00

  • The effect of environmental odors emanating from commercial swine operations on the mood of nearby residents.

    abstract::The effect of environmental odors emanating from large-scale hog operations on the mood of nearby residents was determined using the POMS (Profile of Mood States). The scores for six POMS factors and the TMD (total mood disturbance score) for 44 experimental subjects were compared to those of 44 control subjects who w...

    journal_title:Brain research bulletin

    pub_type: 临床试验,杂志文章


    authors: Schiffman SS,Miller EA,Suggs MS,Graham BG

    更新日期:1995-01-01 00:00:00

  • Anxiogenic effects in benzodiazepine withdrawal are linked to the development of tolerance.

    abstract::Rats were tested in the elevated plus-maze test of anxiety after a probe injection of chlordiazepoxide (CDP 5 mg/kg) following short-term (5 days) or long-term (20 days) pretreatment with 5 or 20 mg/kg/day. After short-term pretreatment with either dose, the probe dose had anxiolytic effects (it increased the % number...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: File SE,Baldwin HA,Aranko K

    更新日期:1987-11-01 00:00:00

  • Origins of serotonergic projections to the lumbar spinal cord in the monkey using a combined retrograde transport and immunocytochemical technique.

    abstract::A newly developed technique employing the retrograde transport of horseradish peroxidase combined with immunocytochemistry is used to identify the cells of origin of descending spinal pathways and their putative neurotransmitters. With this technique the brainstem origins of descending serotonergic (5HT) pathways to t...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Bowker RM,Westlund KN,Coulter JD

    更新日期:1982-07-01 00:00:00

  • 17 beta-Estradiol inhibits the production of dopamine by the tuberoinfundibular dopaminergic neurons of the male rat.

    abstract::Previous work in our laboratory suggests that the indirect stimulation of prolactin gene transcription observed in male rats between 3 and 6 h following estrogen injection results from an inhibition of dopamine release from the tuberoinfundibular dopaminergic neurons. To evaluate further the role of dopamine in the re...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Shaw-Bruha CM,Happe HK,Murrin LC,Rodriguez-Sierra JF,Shull JD

    更新日期:1996-01-01 00:00:00

  • Behavior and physiology of the macaque vestibulo-ocular reflex response to sudden off-axis rotation: computing eye translation.

    abstract::The vestibulo-ocular reflex (VOR) has historically been considered a computationally simple reflex: to stabilize images on the retina against imposed head rotation, the eyes must be counterrotated by an equal amount in the opposite direction. During almost any head rotation, however, the eyes are also translated. We s...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Snyder LH,King WM

    更新日期:1996-01-01 00:00:00

  • Sadness as a passion of the soul: a psychopathological consideration of the Cartesian concept of melancholy.

    abstract::The relationship between the "passions" (emotions or feelings) and psychopathology has been a constant throughout the history of medicine. In this context, melancholy was considered a perversion of the soul (corruption of the passions). One of the most influential authors on this subject was René Descartes, who discus...

    journal_title:Brain research bulletin

    pub_type: 历史文章,杂志文章,评审


    authors: López-Muñoz F,Rubio G,Molina JD,Alamo C

    更新日期:2011-04-25 00:00:00

  • Tamoxifen and mifepriston modulate nicotine induced conditioned place preference in female rats.

    abstract::An increasing number of studies suggest that nicotine/tobacco addiction is modulated by ovarian hormones. The levels of estrogen and progesterone appear to be important in the success of quit attempts and smoking cessation. In women smokers with the diagnosis or risk of breast cancer, the estrogen receptor modulator t...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Yararbas G,Pogun S

    更新日期:2011-04-05 00:00:00

  • lncRNA Mtss1 promotes inflammatory responses and secondary brain injury after intracerebral hemorrhage by targeting miR-709 in mice.

    abstract::Secondary brain injuries following intracerebral hemorrhage (ICH) are mediated by inflammatory pathway activation. The present study aimed to characterize long noncoding RNAs (lncRNAs) that are differentially expressed in cerebral tissues during ICH pathogenesis and to investigate their pathogenic functions. An ICH mo...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Chen JX,Wang YP,Zhang X,Li GX,Zheng K,Duan CZ

    更新日期:2020-09-01 00:00:00

  • Electrophysiology of neural units in goldfish optic tectum.

    abstract::Axons of retinal ganglion cells showed responses not previously emphasized: (a) many tonic units discharged oscillations, 2--12 spikes per burst, interburst intervals 20--300 msec; (b) phasic units showed concentric or flanking ON and OFF fields, response frequency depended on balance of retinal excitation and inhibit...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: O'Benar JD

    更新日期:1976-11-01 00:00:00

  • Sensitivity of lateral hypothalamic neurons to glycemic level: possible involvement of an indirect adrenergic mechanism.

    abstract::Most of the lateral hypothalamic neurons responding to moderate changes in blood glucose fail to be affected by direct glucose applications. Therefore their sensitivity to glycemic level must be mediated by an indirect mechanism. In order to test whether adrenergic afferents might be involved, the activity of lateral ...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Orsini JC,Wiser AK,Himmi T,Boyer A,Perrin J

    更新日期:1991-04-01 00:00:00

  • Age-related quantitative changes in mitochondria of satellite cell sheaths enveloping spinal ganglion neurons in the rabbit.

    abstract::We studied mitochondria in the satellite cell sheaths which envelope the spinal ganglion neurons of rabbits aged 12, 42, and 79 months. While the mean cytoplasmic volume of satellite cell sheaths did not change significantly with age, the mean percentage of cytoplasmic volume occupied by mitochondria decreased with ag...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Martinelli C,Sartori P,Ledda M,Pannese E

    更新日期:2003-07-15 00:00:00

  • Longitudinal analysis of the behavioural phenotype in Hdh(CAG)150 Huntington's disease knock-in mice.

    abstract::In people with Huntington's disease, an expanded CAG repeat sequence on the HTT gene confers a toxic gain function resulting in a progressive and fatal neurodegeneration. The Hdh((CAG)Q150) Huntington's disease mouse line is a knock-in model of the disease that carries ∼150 CAG repeats on the normal mouse Htt locus. T...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Brooks S,Higgs G,Jones L,Dunnett SB

    更新日期:2012-06-01 00:00:00

  • Somatic labeling of the medial terminal nucleus of the accessory optic system with WGA-HRP injected into the rat occipital cortex.

    abstract::Neuronal cell bodies in the medial terminal nucleus of the accessory optic system (MTN) were labeled with WGA-HRP which was injected ipsilaterally into the occipital cortex in the rat. We suggest that the label was first transported anterogradely to the pretectal nucleus of the optic tract, then moved transneuronally ...

    journal_title:Brain research bulletin

    pub_type: 杂志文章


    authors: Takada M,Li ZK,Hattori T

    更新日期:1987-01-01 00:00:00