Abstract：
:Serotonin 5-HT1A receptors belong to the superfamily of G-protein-coupled receptors. Receptor activation of G-proteins can be determined by agonist-stimulated [35S]GTPgammaS binding in the presence of excess GDP, and in vitro autoradiographic adaptation of this technique allows visualization of receptor-activated G-proteins in tissue sections. The present study was performed to examine 5-HT1A receptor activation of G-proteins using 8-OH-DPAT-stimulated [35S]GTPgammaS binding in membranes and brain sections. In hippocampal membranes, 8-OH-DPAT stimulated [35S]GTPgammaS binding by twofold, with an ED50 value of 25 nM. 5-HT1 antagonists, but not 5-HT2 antagonists, increased the ED50 of 8-OH-DPAT in a manner consistent with competitive antagonists. Scatchard analysis of [35S]GTPgammaS binding showed that 8-OH-DPAT induced the formation of high affinity [35S]GTPgammaS binding sites with a KD for GTPgammaS of 3.2 nM. [35S]GTPgammaS autoradiography, performed in brain sections with the 5-HT1A agonist 8-OH-DPAT, revealed high levels of 5-HT1A-stimulated [35S]GTPgammaS binding in the hippocampus, lateral septum, prelimbic cortex, entorhinal cortex, and dorsal raphe nucleus. 5-HT1A-stimulated [35S]GTPgammaS binding in sections was blocked by the addition of the 5-HT1 antagonist methiothepin. These results show that the use of agonist-stimulated [35S]GTPgammaS autoradiography for the 5-HT1A receptor system should provide new information regarding signal transduction in specific brain regions.
journal_name
Brain Res Bulljournal_title
Brain research bulletinauthors
Sim LJ,Xiao R,Childers SRdoi
10.1016/s0361-9230(97)00061-0subject
Has Abstractpub_date
1997-01-01 00:00:00pages
39-45issue
1eissn
0361-9230issn
1873-2747pii
S0361-9230(97)00061-0journal_volume
44pub_type
杂志文章abstract：:This study aimed to assess whether changes in the patterns of local field potential (LFP) oscillations of the subthalamic nucleus (STN) underlie to the clinical improvement within 60 s after turning off subthalamic DBS. We studied by spectral analysis the STN LFPs recorded in 13 nuclei from 7 patients with Parkinson's...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2005.11.012
更新日期：2006-03-31 00:00:00
abstract：:Walker 256 carcinosarcoma cells produce subpleural pulmonary metastases when given intravenously to the Sprague-Dawley rat. The number of metastases increases when the rat is given morphine subsequent to the tumor load. The increase in the number of metastases can be blocked be pretreatment with the opiate antagonist ...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(86)90057-2
更新日期：1986-03-01 00:00:00
abstract：:Electrogastrography (EGG) is a noninvasive measurement of stomach activity using surface electrodes positioned over the abdominal surface. For over 10 years, EGG has been used as an objective measure of epigastric symptoms and nausea experienced in visually induced sickness provoked by circularvection. It was reported...
journal_title：Brain research bulletin
pub_type： 杂志文章,评审
doi：10.1016/s0361-9230(98)00095-1
更新日期：1998-11-15 00:00:00
abstract：:Brain serotonin (5-HT) levels and accumulation rate within the dorsal raphe nucleus (DR) were elevated in ovariectomized (OVX) rats after ovarian steroid treatment, which consisted of estradiol benzoate (EB) followed by progesterone (P) 48 hr later. Some animals were also given the monoamine oxidase (MAO) inhibitor pa...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(81)90111-8
更新日期：1981-12-01 00:00:00
abstract：:In the present study, multi-electrode array recording was used to examine dorsal horn activity following stimulation of primary afferents in a rat dorsal root attached-spinal cord slice preparation. The multi-electrode array probe was placed under the dorsal horn slice and local field potentials evoked by stimulation ...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2017.04.012
更新日期：2017-05-01 00:00:00
abstract：:Neutral, magnesium-stimulated sphingomyelinase and acid sphingomyelinase activities in the neurohypophysis, adenohypophysis and cerebrum of rats given 2.25% NaCl drinking water for 7 or 14 days were compared with the same enzyme activities in the tissues of control animals allowed free access to distilled drinking wat...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(83)90028-x
更新日期：1983-05-01 00:00:00
abstract：:Regenerative medicine strategies rely on exogenous cell transplantation and/or endogenous cell stimulation. Biomaterials can help to increase the regenerative potential of cells and biomolecules by controlling transplanted cell fate and provide a local, sustained release of biomolecules. In this review, we describe th...
journal_title：Brain research bulletin
pub_type： 杂志文章,评审
doi：10.1016/j.brainresbull.2019.03.005
更新日期：2019-05-01 00:00:00
abstract：:One of the important factors altering the degree of blood-brain barrier (BBB) disruption in cerebral ischemia is the anesthetic used. The phosphoinositide 3-kinase (PI3K)/Akt signaling pathway has been reported to be involved in modulating BBB permeability and in isoflurane induced neuroprotection. This study was perf...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2017.02.007
更新日期：2017-05-01 00:00:00
abstract：:After sustaining a traumatic brain injury (TBI), a person's ability to make daily decisions can be affected. Simple tasks such as, deciding what to wear are no longer effortless choices, but are instead difficult decisions. This study explored the use of a discrimination task with a magnesium treatment in order to exa...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2018.02.019
更新日期：2018-05-01 00:00:00
abstract：:Catecholaminergic systems, specifically in the region of the lateral perifornical hypothalamus (PFH), have been linked to the inhibition of feeding behavior. The present studies examined the effects of d-amphetamine (AMPH), which is believed to act through the release of endogenous catecholamines (CAs), on spontaneous...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(86)90200-5
更新日期：1986-11-01 00:00:00
abstract：:In the present study, we showed for the first time the localization of ZNT7 immunoreactivity in the mouse dorsal root ganglion (DRG) by means of immunohistochemistry and confocal laser scanning microscopy. Our results revealed that ZNT7 immunoreactivity was abundantly expressed in the nerve cells of the mouse DRG. Str...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2007.06.022
更新日期：2007-09-28 00:00:00
abstract：:We previously reported that a low threshold action of norepinephrine (NE) on the cerebellar circuitry is expressed as an amplification of the inhibitory action of gamma aminobutyric acid (GABA) on Purkinje cell activity. Here we examined the effects of locus coeruleus (LC) stimulation on "off-beam" inhibitions of Purk...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(83)90211-3
更新日期：1983-06-01 00:00:00
abstract：:The effects of cholinergic agents on isolated dentate neurons were studied to characterize cellular mechanisms underlying carbachol-induced 'theta' EEG activity. Carbachol, eserine, and acetylcholine produced a synchronization of slow wave activity (theta) accompanied by depression of perforant path to dentate field p...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(87)90029-3
更新日期：1987-01-01 00:00:00
abstract：:To investigate nitric oxide production in the brain of the EL mouse, an inbred mutant strain of the ddY mouse that is susceptible to convulsive seizures, we measured whole brain nitric oxide metabolites, and counted the number of nitric oxide-producing cells in the parietal cortex and striatum. Nitric oxide metabolite...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(99)00194-x
更新日期：1999-11-01 00:00:00
abstract：:The effects of changes in ambient and central temperature, amines, PGEu and pyrogen were investigated with respect to the mechanism of Na+-Ca++ ratio in the posterior hypothalamus of the unrestrained cat. Guide tubes were implanted bilaterally above the posterior hypothalamic area of 23 cats so as to accommodate push-...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(76)90102-7
更新日期：1976-05-01 00:00:00
abstract：:The labelling of hypothalamic binding sites by [125I]-FK, a specific mu-opioid receptor ligand, was studied in female C57BL/6J mice to test whether removal of ovarian steroids affected the density of distribution of receptor binding. Labelling densities in the forebrain of normally cycling (intact) females (N = 12), w...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(93)90093-q
更新日期：1993-01-01 00:00:00
abstract：:Recent findings in epigenetics shed new light on the regulation of gene expression in the central nervous system (CNS) during stress. The most frequently studied epigenetic mechanisms are DNA methylation, histone modifications and microRNA activity. These mechanisms stably determine cell phenotype but can also be resp...
journal_title：Brain research bulletin
pub_type： 杂志文章,评审
doi：10.1016/j.brainresbull.2013.07.003
更新日期：2013-09-01 00:00:00
abstract：:The dynamics of hypothalamic arginine vasopressin (AVP) release was examined during the development of hypertension in rats with deoxycorticosterone (DOCA)-NaCL induced hypertension. Experiments were performed in four groups of uninephrectomized Sprague-Dawley rats 3, 7, 14 and 21 days after treatment with: (I) DOCA a...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(86)90016-x
更新日期：1986-01-01 00:00:00
abstract：:Cultures of endothelial cells (EC) derived from human (autopsy) and canine brain microvessels were characterized with respect to growth, morphology, and biochemical features. The endothelial nature of these cells was confirmed by analyses of angiotensin-converting enzyme activity, Factor VIII-related antigen, and ultr...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(88)90047-0
更新日期：1988-11-01 00:00:00
abstract：:Following main olfactory bulb (MOB) stimulation at frequencies of 0.1-0.3 Hz, in addition to early field potentials, a frequency-sensitive, surface negative late N2 wave (latency range: 63-96 msec) followed occasionally by a late N3 transient, was evoked in the piriform cortex and endopiriform nucleus of the rat. The ...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(88)90133-5
更新日期：1988-04-01 00:00:00
abstract：:Trisomy of the whole or distal part of human chromosome 21 (HSA 21) (Ts21) results in Down Syndrome (DS), which is characterized in part by mental retardation and associated neurological abnormalities. Structural abnormalities observed frequently include reduced brain weight, decreased number and depth of sulci in the...
journal_title：Brain research bulletin
pub_type： 杂志文章,评审
doi：10.1016/0361-9230(86)90074-2
更新日期：1986-06-01 00:00:00
abstract：:The beta-galactosidase protein generated by the bacterial LacZ gene is widely used to map gene expression patterns. The ease of its use is only rivaled by green fluorescent protein, which can be used in combination with various other procedures such as immunocytochemistry, flow cytometry, or tract tracing. The beta-ga...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2005.11.007
更新日期：2006-06-15 00:00:00
abstract：:In the central nervous system cell migration is usually restricted to developmental periods and occurs mainly radially, following the radial glia. Nevertheless, in the subependymal layer of the adult rodent forebrain tangential migration of newly generated neuronal precursors directed to the olfactory bulb, which foll...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(96)00116-5
更新日期：1997-01-01 00:00:00
abstract：:There are cholinergic inputs responsible for pressor responses in the rostral ventrolateral medulla (RVLM) and stimulation of midbrain central gray (CG) increases arterial pressure via activation of neurons in the RVLM. In this study, we examined whether the CG was involved in mediation of the cholinergic inputs to th...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/s0361-9230(99)00088-x
更新日期：1999-09-01 00:00:00
abstract：:The effects of systemic injections of amphetamine sulfate on the extracellular levels of glycine, GABA, and taurine in the neostriatum of awake rats were studied using a push-pull perfusion system. Amphetamine produced a dose-related increase in glycine levels. Amphetamine also produced an enhancement on GABA and taur...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(93)90222-w
更新日期：1993-01-01 00:00:00
abstract：:The mechanism of action of the anticonvulsant drug valproic acid (VPA) was studied in rat amygdaloid slices using intracellular recording techniques. In the presence of bicuculline (20 microM), stimulation of the endopyriform nucleus evoked an excitatory postsynaptic potential (EPSP) followed by a paroxysmal depolariz...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(94)90202-x
更新日期：1994-01-01 00:00:00
abstract：:Degeneration of substantia nigra dopaminergic neurons is a key pathological change of Parkinson's disease (PD), and its motor consequences have been widely recognized. Recently, mood disorders associated with PD have begun to attract a great deal of interest, however, their pathogenesis remains unclear. PD is associat...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2014.11.006
更新日期：2015-01-01 00:00:00
abstract：:Insulin release was examined in human subjects after lingual applications of D-glucose and its anomers. A dose-dependent increase in the insulin release was noted following the application of equilibrated D-glucose consisting of 36% alpha- and 64% beta-anomer. The response was not reproduced after administration of at...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/0361-9230(86)90126-7
更新日期：1986-08-01 00:00:00
abstract：:Diabetes mellitus (DM) is considered the primary cause of neuropathic pain. Osthole (7-methoxy-8[3-methylpent 2-enyl]coumarin) is a component extracted from Cnidium monnieri (L.) cusson plant seeds and has anti-inflammatory and anti-oxidative properties. The aim of the present study was to investigate the effects of o...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2018.08.008
更新日期：2018-09-01 00:00:00
abstract：:Homocysteine-inducible endoplasmic reticulum stress-inducible ubiquitin-like domain member 1 protein (HERPUD1) is involved in endoplasmic reticulum stress response. Immense amounts of research showed HERPUD1 plays multiple roles in various models. In this work, we explored the role of HERPUD1 during the pathophysiolog...
journal_title：Brain research bulletin
pub_type： 杂志文章
doi：10.1016/j.brainresbull.2016.11.006
更新日期：2017-01-01 00:00:00
