Abstract:
:Bacteroides forysthus has been shown to be prevalent among patients with periodontitis. Conventional microbiological methods used to identify this bacterium, however, are laborious and time-consuming and are therefore not well-suited for screening purposes. We have developed a polymerase chain-reaction (PCR) method which is rapid, specific, and simple to perform and does not require other sample pre-treatment except a brief centrifugation. This method was applied to the detection of B. forsythus in subgingival plaque of 58 periodontitis patients. When compared with the results of conventional culturing, the PCR method always confirmed the culture-positive results, while none of the PCR negative samples was shown to be culture-positive. The PCR method appeared to give more than double the number of samples positive for B. forsythus than culturing (89.7% vs. 37.9%). The analysis requires less than 4 hrs to perform, and is specific only to B. forsythus and sensitive enough to detect fewer than 5 bacteria.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Meurman JH,Wahlfors J,Korhonen A,Alakuijala P,Väisänen P,Torkko H,Jänne Jdoi
10.1177/00220345970760070701subject
Has Abstractpub_date
1997-07-01 00:00:00pages
1376-80issue
7eissn
0022-0345issn
1544-0591journal_volume
76pub_type
杂志文章abstract::Estrogen deficiency results in disruption of maxillary alveolar bone microarchitecture. Most of the actions of estrogen in long bones occur via estrogen receptor α (ERα). However, the function of ERα in the maxillary bone has not been defined. We aimed to investigate the role and underlying mechanisms of ERα in the ph...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034516633154
更新日期:2016-06-01 00:00:00
abstract::A frameshift mutation recently identified within the paired domain of the transcription factor, PAX9, has been linked to a unique form of oligodontia in a single, multigenerational family (Stockton et al., 2000). We now describe the phenotypic and segregation analyses of this remarkable kindred, the initial approach t...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345000790070701
更新日期:2000-07-01 00:00:00
abstract::Much research has been devoted to the study of etched enamel, since it is critical to bonding. Currently, there are no precise data regarding the etched-enamel specific surface area. The aim of this study was to characterize, by two different methods, the surface of human dental enamel in vitro after being etched. It ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910808700607
更新日期:2008-06-01 00:00:00
abstract::Breastfeeding is a powerful health-promoting behavior. A 2016 Lancet global collaboration to review the health implications of breastfeeding was among the first to consider oral health outcomes. While a role was suggested for breastfeeding in preventing malocclusion, caries was the only included disease condition unfa...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/0022034517738925
更新日期:2018-03-01 00:00:00
abstract::Dental porcelains rely on the high-thermal-expansion mineral leucite to elevate their bulk thermal expansion to levels compatible with dental PFM alloys. The microcracks that form around these leucite particles when cooled during porcelain manufacture are a potential source of change in bulk porcelain thermal expansio...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345960750070801
更新日期:1996-07-01 00:00:00
abstract::Periodontal diseases are initiated by bacteria that accumulate in a biofilm on the tooth surface and affect the adjacent periodontal tissue. Systemic diseases such as diabetes, rheumatoid arthritis (RA), and systemic lupus erythematosus (SLE) increase susceptibility to destructive periodontal diseases. In human studie...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/0022034518805739
更新日期:2019-02-01 00:00:00
abstract::The purpose of this investigation was to examine the inhibitory effects of aqueous extracts derived from the bark-containing sticks (Neem stick) of Azadirachta indica upon bacterial aggregation, growth, adhesion to hydroxyapatite, and production of insoluble glucan, which may affect in vitro plaque formation. Neem sti...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345960750021301
更新日期:1996-02-01 00:00:00
abstract::Rat parotid secretory granule membranes were examined for the presence of calcium-dependent protein kinase activities and kinase substrates. Protein kinase C (C-kinase), which is stimulated by certain phospholipids, was present in the membranes, as indicated by its ability to catalyze the phosphorylation of histone. T...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345870660022901
更新日期:1987-02-01 00:00:00
abstract::Heterozygous mutations in the RUNX2 (CBFA1) gene cause cleidocranial dysplasia, characterized by multiple supernumerary teeth. This suggests that Runx2 inhibits successional tooth formation. However, in Runx2 knockout mice, molar development arrests at the late bud stage, and lower molars are more severely affected th...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910508400206
更新日期:2005-02-01 00:00:00
abstract::In this study, we sought to determine the association between tooth agenesis and DNA sequence variation in the genes MSX1 and PAX9 in an ethnically diverse human population. Since cleft lip/palate is also associated with both tooth agenesis and the gene TGFA, we included TGFA in the analysis as well. Cheek swab sample...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910408300913
更新日期:2004-09-01 00:00:00
abstract::During the polymerization of multifunctional monomers for dental restorations, typical final double-bond conversions range from 55 to 75%. The low conversion results in a large amount of extractable monomer, reduced adhesion to the filler, and the potential for increased swelling. In this work, the ability to increase...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345960750081301
更新日期:1996-08-01 00:00:00
abstract::In order for the role of parotid amylase in starch digestion in the gastro-intestinal tracts of diabetic rats to be clarified, this study investigated the effect of parotid-duct ligation on both amylase secretion from the parotid glands and pancreas into the gastro-intestinal tract and on starch digestion in the gastr...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345890680091501
更新日期:1989-09-01 00:00:00
abstract::Over the last hundred years, groundbreaking research in oral microbiology has provided a broad and deep understanding about the oral microbiome, its interactions with our body, and how the community can affect our health, be protective, or lead to the development of dental diseases. During this exciting journey, hypot...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034519830686
更新日期:2019-04-01 00:00:00
abstract::In a previous experiment, we found that masseter muscle spindles show functional plasticity after 5 to 15 days under increased occlusal vertical dimension (iOVD). In the present study, we hypothesized that spindle function would eventually recover if longer observation periods were allowed. Therefore, in this study we...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910608500914
更新日期:2006-09-01 00:00:00
abstract::Our long-term objective is to devise methods to improve osteotomy site preparation and, in doing so, facilitate implant osseointegration. As a first step in this process, we developed a standardized oral osteotomy model in ovariectomized rats. There were 2 unique features to this model: first, the rats exhibited an os...
journal_title:Journal of dental research
pub_type: 杂志文章,多中心研究
doi:10.1177/0022034517742631
更新日期:2018-04-01 00:00:00
abstract::Dental amalgam restorations are a significant source of mercury exposure in the human population, but their potential to cause systemic health effects is highly disputed. We examined effects on the immune system by giving genetically mercury-susceptible Brown Norway (BN) rats and mercury-resistant Lewis (LE) rats silv...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345980770060601
更新日期:1998-06-01 00:00:00
abstract::Although osteoporosis is a major public health concern, the effect of this condition on oral bone has not been determined. Using the ovariectomized rat as a model, we examined whether acute estrogen depletion affects the mandible of the ovariectomized rat with and without maxillary molar extraction. Twenty-two ovariec...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345940730110901
更新日期:1994-11-01 00:00:00
abstract::The incisor dentin of hypophosphatemic (Hyp) mice was examined histopathologically to determine whether the multiple occurrences of interglobular dentin would be influenced by the serum phosphate level. Both normal and Hyp mice (12 weeks of age) were divided into two groups. The mice in one group were given a control ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345920710031101
更新日期:1992-03-01 00:00:00
abstract::The aim of this study was to investigate whether the release of fluoride and the antimicrobial effect of freshly mixed glass ionomers could be prolonged by application of fluoride on aged material. Test slabs of freshly mixed and aged (14 d in water) conventional and silver glass ionomer (Ketac-Fil and Ketac-Silver, E...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345930720090901
更新日期:1993-09-01 00:00:00
abstract::The temporal and spatial patterns in which teeth are initiated in the growing jaws of embryos are constant for a species but different for different species. The sources of the patterns have been explained in two ways. First, they are the outcome of reactions between molecules created at stationary targets and those w...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345980770090901
更新日期:1998-09-01 00:00:00
abstract::Angiogenesis and biomineral substrates play major roles in bone development and regeneration. We hypothesized that macroporous scaffolds of biomineralized 85:15 poly(lactide-co-glycolide), which locally release vascular endothelial growth factor-165 (VEGF), would direct simultaneous regeneration of bone and vascular t...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910408300304
更新日期:2004-03-01 00:00:00
abstract::Improved mechanical properties of resin composites have been reported to be a consequence of the addition of aldehyde to the resins. The objective of the present study was to examine whether this improvement could be attributed to an increased degree of conversion of double bonds. For this purpose, propanal was added ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345940730101101
更新日期:1994-10-01 00:00:00
abstract::During porcelainization the non-preoxidized alloy develops a Be-rich reaction zone in the interface which separates a Be-depleted alloy from a Li and (to a lesser extent Na) depleted region of the ceramic. Thus, Be diffuses to the interface through the alloy Be degrees leads to Be++ + 2 e- occurs at the interface; som...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345780570021501
更新日期:1978-02-01 00:00:00
abstract::Acculturation is a complex phenomenon that can serve as a proxy for cultural norms and behaviors affecting care-seeking, prevention behaviors, and, ultimately, health outcomes. The purpose of this study was to assess the effect of acculturation on the oral health of Haitian immigrants in New York City. We hypothesized...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910408300219
更新日期:2004-02-01 00:00:00
abstract::The aims of this work were (a) to assess the oral bioavailability of fluoride delivered from dentifrices and (b) to test for a possible link between the results and clinical data obtained with the same dentifrices. Oral fluoride concentrations were measured in samples of saliva and plaque taken from seven subjects, af...
journal_title:Journal of dental research
pub_type: 临床试验,杂志文章,评审
doi:10.1177/002203459207100S09
更新日期:1992-04-01 00:00:00
abstract::In the absence of active pulmonary disease, mycobacterial infection frequently causes arthritis and can be considered to initiate autoimmune diseases such as rheumatoid arthritis. Temporomandibular disorder (TMD) is a disease in which pain and impaired mandibular movement appear to arise directly from degenerative or ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345000790100401
更新日期:2000-10-01 00:00:00
abstract::Multiple interactions occur among major determinants of dental caries. We have studied the bacterial flora and pH-lowering capacity of the same dental plaques in relation to caries. The findings on the plaque flora are reported here. The buccal surfaces of upper teeth in each subject were selected for study. A low-car...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345000790021201
更新日期:2000-02-01 00:00:00
abstract::The periodontium contains heterogeneous mesenchymal cell populations with various differentiation potentials. The capacity of these cells for tissue formation as well as the origin of their precursors are still not entirely defined. In this study, cells originating from different periodontal tissues were cultured in v...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345950740051201
更新日期:1995-05-01 00:00:00
abstract::Genetic studies of early-onset periodontitis (EOP) are hampered by several factors. These include delayed onset of the trait, an upper age limit of expression of the disease, and lack of phenotypic information for edentulous family members. Segregation analyses of families with EOP support a major locus hypothesis but...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345000790031201
更新日期:2000-03-01 00:00:00
abstract::A procedure for measuring nasal airway resistance using posterior rhinorheometry is described. Baseline nasal airway resistances +/- 1 S.D. at an airflow of 0.5 L/sec were found to be 1.077 +/- 0.683 cm H2O for inhalation and 1.359 +/- 0.792 cm H2O for exhalation. Application of either cold water (4 to 6 C) or warm wa...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345770560110301
更新日期:1977-11-01 00:00:00