Abstract:
OBJECTIVE:MIB 1 is a new monoclonal antibody which recognizes nuclei of proliferating cells throughout the cell cycle except during the G0 and early G1 phases. In the present study we analyzed the MIB 1 immunostaining as an index of cellular proliferation in cervical intraepithelial neoplasia (CIN) and microinvasive carcinoma, with the aim to identify a relationship with the degree of dysplastic lesion and the risk of neoplastic progression. A correlation between the MIB 1 index and human papillomavirus (HPV) DNA presence was also investigated METHODS:Cervical bioptic samples were consecutively obtained from 86 women who attended our Colposcopic Service from January 1993 to June 1994, because of abnormal pap smears suspicious for cervical dysplasia and/or HPV infection. On histologic evaluation, 41 women had CIN, 23 cervical condyloma, and 22 squamous metaplasia. Ten patients with microinvasive squamous cervical carcinoma, matched for age and demographic characteristics, were selected from our series of cervical carcinomas and immunohistochemically analyzed. The expression of primary tumor cellular proliferation was immunohistochemically evaluated by monoclonal MIB 1 antibody (Immunotech, Marseille Cedex, France) on microwave oven-processed formalin-fixed paraffin-embedded tissue. Positive staining was expressed as the percentage of positive cells per 10(3) counted dysplastic cells for each case. RESULTS:A progressive significant increase in positive MIB 1 immunostaining was observed from squamous metaplasia to microinvasive carcinoma throughout the CIN lesions (p < 0.001). Considering only CINs, the MIB 1 index showed a significant increase with respect to CIN degrees (p < 0.001); no correlation was found between MIB 1 immunostaining and HPV infection, and lesion size. By analyzing the MIB 1 index with respect to CIN outcome in mild and moderate dysplasias, regressive lesions had lower values of MIB 1 immunostaining, while persistent and progressive lesions presented significantly higher positivity (p < 0.001). CONCLUSIONS:Our data demonstrated: (1) that positive MIB 1 immunostaining increased progressively from squamous metaplasia to CIN and microinvasive carcinoma, suggesting that neoplastic transformation is associated with a dysfunctional proliferation of cervical epithelium; (2) that there was a significant correlation between the MIB 1 index and CIN degree but not with respect to HPV DNA presence, and (3) that MIB 1 immunostaining might be useful for a clinical evaluation of mild and moderate dysplastic lesions. However, a much larger study needs to be done over a longer period of time to truly determine the value of the technique in prognostically predicting which lesions might or might not regress.
journal_name
Gynecol Obstet Investjournal_title
Gynecologic and obstetric investigationauthors
Garzetti GG,Ciavattini A,De Nictolis M,Lucarini G,Goteri G,Romanini C,Biagini Gdoi
10.1159/000291977subject
Has Abstractpub_date
1996-01-01 00:00:00pages
261-6issue
4eissn
0378-7346issn
1423-002Xjournal_volume
42pub_type
杂志文章abstract::The objective of the study was to assess the effects of low-dose vaginal treatment with oestradiol before vaginal operation. In a double-blind randomized study including 43 postmenopausal women scheduled for vaginal repair operation for genital descensus, it was found that 7 patients suffered from concomitant urinary ...
journal_title:Gynecologic and obstetric investigation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000292320
更新日期:1995-01-01 00:00:00
abstract::We report the third case of a prenatal diagnosis of Larsen's syndrome, which is the first report affecting both a 37-year-old primiparous caucasian woman and her fetus not considered to have Larsen's syndrome until the finding of bilateral clubfeet was demonstrated on screening ultrasound at 23 weeks of gestation. His...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000010050
更新日期:1998-01-01 00:00:00
abstract::A 29-year-old woman presented with preterm labour at 32 weeks of gestation. Tocolytic treatment was started with intravenous hexoprenaline. Twenty-four hours after initiation of treatment, the patient developed supraventricular tachycardia, resistant to digoxin and verapamil. Medical treatment with metoprolol finally ...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000292411
更新日期:1995-01-01 00:00:00
abstract:OBJECTIVE:Isotretinoin is used in acne vulgaris treatment for more than 20 years. Isotretinoin has serious side effects on many organs, but there are no comprehensive studies investigating its possible toxic effects on reproductive organs. Thus, we aimed to investigate the possible toxic effects of isotretinoin adminis...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000510031
更新日期:2020-01-01 00:00:00
abstract::To evaluate a new reflectance photometer (Accusport) for blood lactate determination, a prospective non-interventional study was performed on blood obtained from the umbilical cord artery immediately after delivery of the baby in 109 consecutive women. For each sample, the results of cord arterial blood lactate values...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000009919
更新日期:1998-01-01 00:00:00
abstract:AIMS:To investigate the differences in insulin resistance between women with recurrent miscarriage and those with normal pregnancy. METHODS:Pregnant women with a history of recurrent miscarriage were included in the patient group (n = 97), while those with no history of abnormal pregnancy were included in the control ...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000325165
更新日期:2011-01-01 00:00:00
abstract:AIMS:The study aimed to compare the clinical course and disease severity between culture positive and culture negative patients with intra-uterine devices (IUD)-associated pelvic inflammatory disease (PID). METHODS:A retrospective study of all IUD-associated PID patients admitted to tertiary medical center between 201...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000490666
更新日期:2019-01-01 00:00:00
abstract::The adherence of Neisseria gonorrhoeae, Escherichia coli and group B streptococci to vaginal epithelial cells from post-menopausal women was studied by an in vitro test system. It was found that the adherence rate of gonococci to vaginal cells from women on oestrogen treatment was statistically significantly higher (p...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000299855
更新日期:1980-01-01 00:00:00
abstract::The scanning electron microscopy by virtue of its high resolution topographic reproduction of epithelial surfaces lining the female genital tract, provides unique information toward understanding the anatomy and physiology of the female reproductive system. The human Fallopian ectopic pregnancy is an urgent gynecologi...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000299226
更新日期:1983-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the efficacy of depot medroxy progesterone acetate (DMPA) as a preparatory agent for endometrial resection. STUDY DESIGN:Endometrial resection was performed on 50 women for excessive uterine bleeding. The patients were randomly divided into 2 equal groups, with the first group receiving DMPA and ...
journal_title:Gynecologic and obstetric investigation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000052969
更新日期:2001-01-01 00:00:00
abstract::The feasibility of performing hysteroscopic endomyometrial resection using isotonic 2.2% glycine as distension medium was studied in 181 consecutive operations. A standard continuous flow loop-resectoscope was used. Distension was achieved by means of a gravity-fed system and glycine flowed out under its own pressure....
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000010119
更新日期:1999-01-01 00:00:00
abstract::The effects of estradiol-17 beta (E2) and clomiphene citrate (CC) on pituitary-uterine function in ovariectomized (OVX) rats were compared in two experiments. In experiment 1, CC completely blocked E2 stimulation of uterine weight when administered concurrently for 10 and 30 days. In experiment 2, content of total (oc...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000293095
更新日期:1991-01-01 00:00:00
abstract::Full vaginal casts of 39 caucasian women, 13 each nulliparous, uniparous and multiparous, were made using vinyl polysiloxane impression material. Vaginal lengths were measured with polished acrylic rods inserted by the subjects, and introital diameters were measured with conical polypropylene flasks. Parallel sided, c...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000291946
更新日期:1996-01-01 00:00:00
abstract::The relationship between age and urodynamic parameters was studied cross-sectionally in a group of 28 women in whom clinically and urodynamically both stress incontinence and detrusor instability were excluded (no proven incontinence, NPI) and in a group, matched for age, with genuine stress incontinence (GSI). At all...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000294858
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND:We sought to compare the pullout strengths of the sacral end of the sacrocolpopexy mesh when attached using one suture at the first versus second sacral vertebral level (S1 vs. S2) in female cadaveric pelvises. METHODS:The sacral vertebrae were isolated in 9 unembalmed female cadavers, and segments of polyp...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000338892
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND/AIMS:To test the hypothesis that obstetrical disseminated intravascular coagulopathy results from an excessive leakage of fetal material into the maternal circulation. METHODS:All peripartum hysterectomy cases for hemorrhage at two suburban Illinois hospitals over 10 years were included. Intravascular prese...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000332373
更新日期:2012-01-01 00:00:00
abstract:AIM:To compare outcomes after laparoscopic ovarian cystectomy versus fenestration/coagulation or laser ablation for the treatment of endometriomas. METHODS:Studies were identified by searching the PubMed, EMBASE, SCOPUS, and Cochrane Central Register of Controlled Trials databases using the terms ovarian, endometrioma...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章,meta分析
doi:10.1159/000351165
更新日期:2013-01-01 00:00:00
abstract::Our purpose was to determine whether intrauterine sonography with high-frequency, real-time miniature transducer (20 MHz) is useful for the diagnosis of gynecologic disorders. The study consisted of 37 women: 8 normal volunteers, 2 with molar pregnancy, 4 fibromyoma, 4 endometrial polyp, 1 intrauterine adhesion, 1 sep...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000010072
更新日期:1999-01-01 00:00:00
abstract::Human heparanase has been shown to function in tumor progression, metastatic spread, and tumor angiogenesis. The aim of the present study was to assess heparanase expression in endometrial cancer in correlation with neovascularization and clinicopathological factors. Forty endometrial cancers were obtained from previo...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000072821
更新日期:2003-01-01 00:00:00
abstract::We present a case of leiomyomatosis peritonealis disseminata (LPD) after myoma morcellation and review the literature using the keywords leiomyomatosis peritonealis disseminata and disseminated peritoneal leiomyomatosis. The search was conducted in Medline, EMBASE, and Cochrane Database of systematic reviews. We encou...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章,评审
doi:10.1159/000274487
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND/AIMS:A phase II study was conducted to assess the activity and toxicity of weekly docetaxel in patients with advanced or recurrent cancer of the cervix. METHODS:Eligible patients were required to have measurable disease with adequate performance status, bone marrow, renal and hepatic function. Patients were...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000106489
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND/AIMS:To determine the clinicopathological characteristics and potentially associated outcomes in patients diagnosed with serous adenocarcinoma of the uterine cervix. METHODS:The records of surgically-treated patients with pathological stage pT1b-2b serous adenocarcinoma were reviewed. RESULTS:Of 12 patient...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章,评审
doi:10.1159/000329319
更新日期:2012-01-01 00:00:00
abstract::The cardiovascular and metabolic responses during acute fetal hypoxia were studied in 7 pregnant sheep near term. Complete reduction of uterine flow (UBF) for 180 s was performed in 13 experiments by inflating a balloon positioned in the maternal aorta. Fetal heart rate (FHR), systolic and diastolic blood pressure (BP...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000299425
更新日期:1983-01-01 00:00:00
abstract::This study was conducted in order to investigate the usefulness of granulocyte elastase levels as predictive factors in the onset of perinatal infections. The subjects were 41 patients who delivered within 48 h after amniocentesis after giving their informed consent. The relationship between cervical granulocyte elast...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000292401
更新日期:1995-01-01 00:00:00
abstract::Estrogen (ER) - and progestagen (PgR) - receptors were estimated by a DCC method using Scatchard plot analysis In tissue samples of 68 patients with ovarian carcinomas. The mean ER and PgR levels were at 52 and 74 fmol/mg cytosol protein, respectively. ER+ PgR+ records were noted in 32.4%, ER+ PgR- in 26.4%, ER- PgR+ ...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000299256
更新日期:1983-01-01 00:00:00
abstract:BACKGROUND:Genetic factors may play a role in male infertility. METHODS:In a prospective case-control study, we assessed the allele and genotype frequencies of the TNFalpha -308 C-->T and -863 C-->A polymorphisms, detected by PCR of sperm DNA, of 577 Caucasian men recruited in an infertility clinic. Semen sampling was...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000126494
更新日期:2008-01-01 00:00:00
abstract:HYPOTHESIS:The new hysteroscopic system with mechanical energy is an effective outpatient technique for diagnosis and treatment that has certain advantages over conventional hysteroscopy in the management of endometrial polyps. OBJECTIVES:Our primary objective was to assess the total duration of hysteroscopy and polyp...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章,随机对照试验
doi:10.1159/000377700
更新日期:2015-01-01 00:00:00
abstract::The Special Programme of Research in Human Reproduction (HRP), co-sponsored by the UNDP, UNFPA, WHO, and the World Bank, is celebrating 40 years of activities with an expansion of its mandate and new co-sponsors. When it began, in 1972, the main focus was on evaluating the acceptability, effectiveness, and safety of e...
journal_title:Gynecologic and obstetric investigation
pub_type: 历史文章,杂志文章,评审
doi:10.1159/000343067
更新日期:2012-01-01 00:00:00
abstract::Immunocytochemical study in paraffin sections of human endometrium showed that the receptor contents for both oestrogen and progesterone receptors were lower than in the frozen sections although the staining patterns were similar in these two section types. Pretreating the specimens with proteolytic enzymes like tryps...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000292422
更新日期:1995-01-01 00:00:00
abstract::This is a descriptive and cross-sectional study aiming to determine the factors affecting sexual life in pregnant women. The study was carried out in 238 women in Malatya, Turkey. It was found that 61.4% regarded coitus as a risk during pregnancy, 31.9% did not have any knowledge about this matter. In 81.5% sexual lif...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000075582
更新日期:2004-01-01 00:00:00