Abstract:
OBJECTIVE:Isotretinoin is used in acne vulgaris treatment for more than 20 years. Isotretinoin has serious side effects on many organs, but there are no comprehensive studies investigating its possible toxic effects on reproductive organs. Thus, we aimed to investigate the possible toxic effects of isotretinoin administration on oocyte maturation in female rat gonads in this study. METHODS:Thirty-two adolescent female rats (Wistar Albino, 220 ± 35 g) were randomly divided into 4 groups with 8 subjects in each group: group 1, group 2, group 3, and group 4. Different doses of isotretinoin which was dissolved in sesame oil were given to rats by gavage: 7.5 mg/kg/day in group 3 and 15 mg/kg/day in group 4. The rats in group 2 received sesame oil by gavage. To create gavage stress, only gavage was administered to the rats in group 1. The gavages for each group continued once a day and at a certain time for 30 days. To determine the effect of isotretinoin on oocyte maturation, the periodic acid-Schiff reaction was performed for histochemical and histomorphometric evaluation of the zona pellucida, and staining of growth differentiation factor-9 (GDF-9) and bone morphogenetic protein-15 (BMP-15) was performed for immunohistochemical analysis. RESULTS:When the thickness of the zona pellucida was evaluated, a statistically significant difference was found between group 1 and experimental groups (group 3 and group 4). In the experimental groups, it was determined that the thickness of the zona pellucida was decreased depending on the increase in dose. GDF-9 and BMP-15 expressions in oocytes of primordial and primary follicles decreased significantly in the experimental groups compared to group 1 and group 2. However, the expression of GDF-9 and BMP-15 in oocytes of secondary follicles was not significantly different between group 1 and group 2 and the experimental groups. CONCLUSIONS:In our study, we showed toxic effect of isotretinoin on oocyte maturation in female rats.
journal_name
Gynecol Obstet Investjournal_title
Gynecologic and obstetric investigationauthors
Kacamak P,Ozogul C,Saribas GS,Akarca Dizakar SO,Emniyet Sert AAdoi
10.1159/000510031subject
Has Abstractpub_date
2020-01-01 00:00:00pages
327-335issue
4eissn
0378-7346issn
1423-002Xpii
000510031journal_volume
85pub_type
杂志文章abstract::The rate of spontaneous and methotrexate (MTX)-induced chromosome breakage was studied in individuals with a history of habitual abortions in whom a structural chromosomal aberration was found in a single cell during routine cytogenetic analysis. Twelve such individuals were selected because they were not under the in...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000292599
更新日期:1993-01-01 00:00:00
abstract::Fetal echocardiographic and Doppler ultrasonographic prospective studies were performed in utero on 299 babies delivered at Hirata Municipal Hospital, Shimane, Japan, from May 1984 to June 1986. Two or three ultrasonographic examinations were performed on each fetus from 20 weeks of gestation to term. Three congenital...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000293682
更新日期:1988-01-01 00:00:00
abstract::Twenty-four women with second-trimester cervical incompetence underwent emergency cerclage. The appropriateness of cervical cerclage was analyzed according to a scoring system (Cervical Incompetence Scale; CIS) which measures the degree of cervical effacement, cervical dilatation and protrusion of fetal membranes into...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000293093
更新日期:1991-01-01 00:00:00
abstract::Lipid peroxidation and the antioxidant system were investigated in the plasma and placenta of normal and preeclamptic pregnant women. A significant increase in thiobarbituric acid reactive substance (TBARS), significant decreases in total thiol (t-SH) content and superoxide dismutase (SOD) activity, and unchanged vita...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000009994
更新日期:1998-01-01 00:00:00
abstract::Tubal occlusion using Filshie clip is one of the most commonly performed operations for female sterilization. It is usually a simple and safe procedure, and operative complications are uncommon. We report a rare case of spontaneous expulsion of a Filshie clip through the anterior abdominal wall 5 years after steriliza...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章,评审
doi:10.1159/000070615
更新日期:2003-01-01 00:00:00
abstract::Since not all problems in endometriosis can be studied in the human, there is need of an animal model. We transplanted endometrial tissue of the rabbit into the peritoneal cavity and studied the changes after 12 weeks. At that time the endometrial implants in the rabbit had become cystic structures. The macroscopic an...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000293611
更新日期:1989-01-01 00:00:00
abstract::We present an unusual case in whom a small congenital hernia of the umbilical cord and associated patent omphalomesenteric duct were diagnosed at midtrimester ultrasonography. The diagnosis was confirmed following delivery, and neonatal corrective surgery was performed. We compare prenatal ultrasound findings of umbil...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000052895
更新日期:2001-01-01 00:00:00
abstract:AIM:We report a case of a mixed clear cell and endometrioid carcinoma arising in parietal endometriosis. METHODS:A 46-year-old woman presented a second recurrence of parietal endometriosis. An extensive surgical resection of the tumor was then carried out. RESULTS:Histological analysis revealed heterogeneous tissues ...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000096437
更新日期:2007-01-01 00:00:00
abstract:OBJECTIVES:The aim of this study is to evaluate oxidative protein damage (OPD) by investigating protein carbonyl (PCO) and nitrotyrosine (NT) levels, oxidative stress by total thiol (T-SH), erythrocyte glutathione (GSH) and nitric oxide (NO) levels in women receiving hormone replacement therapy (HRT). MATERIALS AND ME...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000067718
更新日期:2002-01-01 00:00:00
abstract::The theory that the Th1 and Th2 cell ratio shifts towards Th2 dominance during pregnancy may improve fetal survival has gained support from recent studies. Also, the variation in the Th1/Th2 cell ratio is reportedly associated with intrauterine growth retardation and preeclampsia. On the basis of these findings, the a...
journal_title:Gynecologic and obstetric investigation
pub_type: 临床试验,杂志文章
doi:10.1159/000010328
更新日期:2000-01-01 00:00:00
abstract::We measured three selected components of the fibrinolytic system in amniotic fluid of 36 parturient women, i.e. the antigen of tissue plasminogen activator (tPA) and antigens of plasminogen activator inhibitor of type 1 (PAI-1) and type 2 (PAI-2). The ELISA method was used. During labour, the level of each of the stud...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000292387
更新日期:1995-01-01 00:00:00
abstract::Full vinyl polysiloxane casts of the vagina were obtained from 23 Afro-American, 39 Caucasian and 15 Hispanic women in lying, sitting and standing positions. A new shape, the pumpkin seed, was found in 40% of Afro-American women, but not in Caucasians or Hispanics. Analyses of cast and introital measurements revealed:...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000010281
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND/AIMS:To evaluate the degree of pain during and after office hysteroscopy with combined intracervical and intrauterine anesthesia compared to intracervical anesthesia only. METHODS:We evaluated the amount of pain experienced during office hysteroscopy using combined local intracervical and intrauterine anest...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000252957
更新日期:2010-01-01 00:00:00
abstract::Circulating levels of leptin, estradiol (E(2)) and progesterone (P(4)) throughout normal menstrual cycles (n = 13), and controlled, stimulated cycles (n = 33) were examined using sensitive specific radioimmunoassays in order to investigate the relationships between sex hormones (E(2), P(4)). Serum leptin levels during...
journal_title:Gynecologic and obstetric investigation
pub_type: 临床试验,杂志文章
doi:10.1159/000010228
更新日期:2000-01-01 00:00:00
abstract::Our objective was to compare ophthalmic artery pulsatility index values from normal pregnant women with those from preeclampsia patients. The ophthalmic artery of 20 normotensive pregnant women, 7 mildly preeclamptic and 2 severely preeclamptic patients was studied once with color Doppler flow imaging and pulsed Doppl...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000292298
更新日期:1995-01-01 00:00:00
abstract:INTRODUCTION:The role of defective fimbrio-ovarian relation in cases of unexplained infertility has not been extensively studied. The aim of the study was to assess the role of fimbrio-ovarian relation as an investigative modality in women with unexplained infertility. We also aimed to evaluate the efficacy of superovu...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000086004
更新日期:2005-01-01 00:00:00
abstract::In 25 pregnant females with a placental weight below the 20th percentile uteroplacental blood flow measurements using 113mIn-transferrin were performed in the 3rd trimester of pregnancy. Twelve patients with pregnancy-induced hypertension (PIH) were compared to 13 females with normal blood pressures. The results of ut...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000298931
更新日期:1986-01-01 00:00:00
abstract::We report the third case of a prenatal diagnosis of Larsen's syndrome, which is the first report affecting both a 37-year-old primiparous caucasian woman and her fetus not considered to have Larsen's syndrome until the finding of bilateral clubfeet was demonstrated on screening ultrasound at 23 weeks of gestation. His...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000010050
更新日期:1998-01-01 00:00:00
abstract:OBJECTIVE:To evaluate alterations in maternal circulating angiogenin levels with advancing gestation and to assess the effect of labor stress on serum angiogenin levels in neonates delivered vaginally and by cesarean section. METHODS:The maternal circulating angiogenin concentrations were compared in 37 normotensive n...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000010270
更新日期:2000-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was determination and comparative analysis of the maternal and umbilical cord sCD40L serum levels in pregnancies complicated by pre-eclampsia with and without intrauterine growth retardation (IUGR) and in normotensive pregnancies. PATIENTS AND METHODS:The study was carried out on 16 pat...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000098316
更新日期:2007-01-01 00:00:00
abstract::Mesh of absorbable polyglactin 910 instead of human tissues or nonabsorbable synthetic materials was utilized for retropubic sling procedures in 21 women with urinary stress incontinence. The patients have been observed for a period ranging from 4 to 14 months. 20 of them are continent while there was 1 relapse after ...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000299212
更新日期:1983-01-01 00:00:00
abstract::Our objective was to evaluate the efficacy and safety of labor induction in women with a breech presentation, and an unripe cervix. We conducted a retrospective, matched-paired study on patients with breech presentation and an unripe cervix (n = 23), who underwent induction of labor using extra-amniotic saline instill...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000010029
更新日期:1998-01-01 00:00:00
abstract::We herein present a case of exencephaly with rachischisis, situs ambiguous and club feet, diagnosed antenatally by ultrasound. This combination of anomalies is very rare. ...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000293305
更新日期:1990-01-01 00:00:00
abstract::The essential metal copper, zinc and iron content in starved virgin and pregnant rat tissue has been studied. The copper content of the whole rat, which was actually increased in pregnant rats, decreased in starved pregnant rats. The differences were significant in 19-day pregnant rats. The total copper content of the...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000293737
更新日期:1988-01-01 00:00:00
abstract:BACKGROUND/AIMS:To compare the efficacy and safety of trocar-site infiltration with lidocaine and no intervention for postoperative pain relief after diagnostic laparoscopy. METHODS:A randomized clinical trial of women with infertility scheduled for diagnostic laparoscopy without additional procedures in Life Institut...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章,随机对照试验
doi:10.1159/000490565
更新日期:2019-01-01 00:00:00
abstract::Implantation of intrauterine devices (IUDs) is associated with an increased incidence of uterine infection, probably as a result of vaginal bacteria entering the uterus at the time of insertion. To reduce the incidence of IUD-related infections, the incorporation of antimicrobial agents into the tail of the device was...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000010256
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND/AIM:We aimed to assess the value of early laparoscopic therapy in management of tubo-ovarian abscess (TOA) or pelvic abscess. METHODS:This was a retrospective study of all consecutive patients who were initially diagnosed with TOA or pelvic abscess at the local hospital between January 2010 and December 201...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000493855
更新日期:2019-01-01 00:00:00
abstract::An evaluation of the age distribution of erythrocytes in twin gestation was performed. Based on previous studies on age distribution of erythrocytes in late pregnancy, we have proposed that a more pronounced shift in age distribution of erythrocytes in twin pregnancy may be expected. We have used the density distribut...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000292618
更新日期:1993-01-01 00:00:00
abstract::Pathologic material was reviewed and survival data were obtained on 182 of the endometrial cancer patients reported in our previous study (1975) to compare histopathologic characteristics and clinical behavior (survival) between endometrial adenocarcinoma in estrogen users and nonusers. In addition, relative risk esti...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000299600
更新日期:1981-01-01 00:00:00
abstract::In a prospective clinical investigation of 2,144 deliveries, we elucidate the indications for episiotomy and how different methods of anesthesia affect the frequency of episiotomy and the perineal problems after episiotomy compared with those after spontaneous perineal laceration. We found a significantly higher infec...
journal_title:Gynecologic and obstetric investigation
pub_type: 杂志文章
doi:10.1159/000293161
更新日期:1991-01-01 00:00:00