Expression of dystrophin Dp71 during PC12 cell differentiation.

Abstract:

:The expression of dystrophin-protein 71 (Dp71) was investigated during nerve growth factor (NGF) induced differentiation of PC12 cells. A semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay was designed to measure Dp71 mRNA, whereas the Dp71 protein amount was evaluated by immunoblot analysis using an anti-dystrophin monoclonal antibody. Comparison with control cultures showed that Dp71 mRNA and protein levels increased in parallel with NGF treatment peaking with increments of 60% and 1.4 times, respectively. The upregulation of Dp71 expression during PC12 cells differentiation point at PC12 cells as a suitable model for studying the function of Dp71 in neuronal cells.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Cisneros B,Rendon A,Genty V,Aranda G,Marquez F,Mornet D,Montañez C

doi

10.1016/0304-3940(96)12863-9

subject

Has Abstract

pub_date

1996-08-02 00:00:00

pages

107-10

issue

2

eissn

0304-3940

issn

1872-7972

pii

0304-3940(96)12863-9

journal_volume

213

pub_type

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