Palmitoylation of the GluR6 kainate receptor.

Abstract:

:The G-protein-coupled metabotropic glutamate receptor mGluR1 alpha and the ionotropic glutamate receptor GluR6 were examined for posttranslational palmitoylation. Recombinant receptors were expressed in baculovirus-infected insect cells or in human embryonic kidney cells and were metabolically labeled with [3H]palmitic acid. The metabotropic mGluR1 alpha receptor was not labeled whereas the GluR6 kainate receptor was labeled after incubation with [3H]palmitate. The [3H]palmitate labeling of GluR6 was eliminated by treatment with hydroxylamine, indicating that the labeling was due to palmitoylation at a cysteine residue via a thioester bond. Site-directed mutagenesis was used to demonstrate that palmitoylation of GluR6 occurs at two cysteine residues, C827 and C840, located in the carboxyl-terminal domain of the molecule. A comparison of the electrophysiological properties of the wild-type and unpalmitoylated mutant receptor (C827A, C840A) showed that the kainate-gated currents produced by the unpalmitoylated mutant receptor were indistinguishable from those of the wild-type GluR6. The unpalmitoylated mutant was a better substrate for protein kinase C than the wild-type GluR6 receptor. These data indicate that palmitoylation may not modulate kainate channel function directly but instead affect function indirectly by regulating the phosphorylation state of the receptor.

authors

Pickering DS,Taverna FA,Salter MW,Hampson DR

doi

10.1073/pnas.92.26.12090

subject

Has Abstract

pub_date

1995-12-19 00:00:00

pages

12090-4

issue

26

eissn

0027-8424

issn

1091-6490

journal_volume

92

pub_type

杂志文章
  • Emergent rules for codon choice elucidated by editing rare arginine codons in Escherichia coli.

    abstract::The degeneracy of the genetic code allows nucleic acids to encode amino acid identity as well as noncoding information for gene regulation and genome maintenance. The rare arginine codons AGA and AGG (AGR) present a case study in codon choice, with AGRs encoding important transcriptional and translational properties d...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1605856113

    authors: Napolitano MG,Landon M,Gregg CJ,Lajoie MJ,Govindarajan L,Mosberg JA,Kuznetsov G,Goodman DB,Vargas-Rodriguez O,Isaacs FJ,Söll D,Church GM

    更新日期:2016-09-20 00:00:00

  • Self-removal of condensed water on the legs of water striders.

    abstract::The ability to control drops and their movements on phobic surfaces is important in printing or patterning, microfluidic devices, and water-repellent materials. These materials are always micro-/nanotextured, and a natural limitation of repellency occurs when drops are small enough (as in a dew) to get trapped in the ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1506874112

    authors: Wang Q,Yao X,Liu H,Quéré D,Jiang L

    更新日期:2015-07-28 00:00:00

  • Cyclophilin catalyzes protein folding in yeast mitochondria.

    abstract::Cyclophilins are a family of ubiquitous proteins that are the intracellular target of the immunosuppressant drug cyclosporin A. Although cyclophilins catalyze peptidylprolyl cis-trans isomerization in vitro, it has remained open whether they also perform this function in vivo. Here we show that Cpr3p, a cyclophilin in...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.92.14.6319

    authors: Matouschek A,Rospert S,Schmid K,Glick BS,Schatz G

    更新日期:1995-07-03 00:00:00

  • Synergistic action of glucocorticoid and estradiol responsive elements.

    abstract::Modulation of gene expression by steroid hormones is mediated by receptor proteins that associate with regulatory elements of responsive genes upon binding the hormone ligand. The finding that two glucocorticoid responsive elements act cooperatively to stimulate transcription of the tyrosine aminotransferase gene prom...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.20.7526

    authors: Ankenbauer W,Strähle U,Schütz G

    更新日期:1988-10-01 00:00:00

  • Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.

    abstract::We describe a technique for transferring electrophoretically separated bands of RNA from an agarose gel to paper strips. The RNA is coupled covalently to diazobenzyloxymethyl groups on the paper. After transfer and appropriate treatment of the paper to destroy remaining diazo groups, specific RNA bands can be detected...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.74.12.5350

    authors: Alwine JC,Kemp DJ,Stark GR

    更新日期:1977-12-01 00:00:00

  • Symplastic signaling instructs cell division, cell expansion, and cell polarity in the ground tissue of Arabidopsis thaliana roots.

    abstract::Cell-to-cell communication is essential for the development and patterning of multicellular organisms. In plants, plasmodesmata (PD) provide direct routes for intercellular signaling. However, the role that PD-mediated signaling plays in plant development has not been fully investigated. To gain a comprehensive view o...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1610358113

    authors: Wu S,O'Lexy R,Xu M,Sang Y,Chen X,Yu Q,Gallagher KL

    更新日期:2016-10-11 00:00:00

  • On the nature of the protein folding code.

    abstract::This paper investigates quantitatively the characteristics of the local folding code. The overlapping four-residue fragments which make up the amino acid sequences of 114 proteins are divided into classes on the basis of the physical properties of their constituent amino acids. The distribution of structural types ass...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.90.2.644

    authors: Rackovsky S

    更新日期:1993-01-15 00:00:00

  • Humans incorporate attention-dependent uncertainty into perceptual decisions and confidence.

    abstract::Perceptual decisions are better when they take uncertainty into account. Uncertainty arises not only from the properties of sensory input but also from cognitive sources, such as different levels of attention. However, it is unknown whether humans appropriately adjust for such cognitive sources of uncertainty during p...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1717720115

    authors: Denison RN,Adler WT,Carrasco M,Ma WJ

    更新日期:2018-10-23 00:00:00

  • A p60 polypeptide in the feline leukemia virus pseudotype of Moloney sarcoma virus with murine leukemia virus p30 antigenic determinants.

    abstract::A 60,000-dalton polypeptide (p60) has been identified in the feline leukemia virus (FeLV) pseudotype of Moloney sarcoma virus [MSV(FeLV)]. This polypeptide is present in the purified virus complex in concentrations greater than either the murine p30 or the feline p27. Purified p60 crossreacts immunologically with muri...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.72.6.2380

    authors: Oskarsson MK,Robey WG,Harris CL,Fischinger PJ,Haapala DK,Vande Woude GF

    更新日期:1975-06-01 00:00:00

  • Presence of corticotropin in brain of normal and hypophysectomized rats.

    abstract::Immunoreactive and bioreactive corticotropin (ACTH-like) activities have been detected in the median eminence and remaining medial basal hypothalamus of both normal and hypophysectomized adult male rats: bioreactive ACTH (pg/100 mug of protein) 1028 in median eminence and 1289 in medial basal hypothalamus; immunoreact...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.74.2.648

    authors: Krieger DT,Liotta A,Brownstein MJ

    更新日期:1977-02-01 00:00:00

  • Cloning of the major histocompatibility complex class II promoter binding protein affected in a hereditary defect in class II gene regulation.

    abstract::The regulation of major histocompatibility complex class II gene expression is directly involved in the control of normal and abnormal immune responses. In humans, HLA-DR, -DQ, and -DP class II heterodimers are encoded by a family of alpha- and beta-chain genes clustered in the major histocompatibility complex. Their ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.86.11.4200

    authors: Reith W,Barras E,Satola S,Kobr M,Reinhart D,Sanchez CH,Mach B

    更新日期:1989-06-01 00:00:00

  • Cross influences of ozone and sulfate precursor emissions changes on air quality and climate.

    abstract::Tropospheric O(3) and sulfate both contribute to air pollution and climate forcing. There is a growing realization that air quality and climate change issues are strongly connected. To date, the importance of the coupling between O(3) and sulfate has not been fully appreciated, and thus regulations treat each pollutan...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0508769103

    authors: Unger N,Shindell DT,Koch DM,Streets DG

    更新日期:2006-03-21 00:00:00

  • Responses of terrestrial ecosystems and carbon budgets to current and future environmental variability.

    abstract::We assess the significance of high-frequency variability of environmental parameters (sunlight, precipitation, temperature) for the structure and function of terrestrial ecosystems under current and future climate. We examine the influence of hourly, daily, and monthly variance using the Ecosystem Demography model ver...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0912032107

    authors: Medvigy D,Wofsy SC,Munger JW,Moorcroft PR

    更新日期:2010-05-04 00:00:00

  • On finitely additive vector measures.

    abstract::In this paper we extend the well-known Vitali-Hahn-Saks and Nikodým theorems for measures to finitely additive vector-valued set functions. ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.67.3.1294

    authors: Brooks JK,Jewett RS

    更新日期:1970-11-01 00:00:00

  • Transplantation of autoimmune potential. I. Development of antinuclear antibodies in H-2 histocompatible recipients of bone marrow from New Zealand Black mice.

    abstract::Antinuclear autoantibodies (ANA) appeared in the plasma of lethally irradiated H-2d histocompatible DBA/2 and BALB/c mice several weeks after intravenous transplantation of 2 to 4 x 10(6) bone marrow cells from 3-week-old animals of the autoimmune New Zealand Black (NZB) strain. Little or no ANA development was observ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.71.6.2162

    authors: Morton JI,Siegel BV

    更新日期:1974-06-01 00:00:00

  • cDNA cloning of somatolactin, a pituitary protein related to growth hormone and prolactin.

    abstract::From a flounder pituitary cDNA library, cDNA clones encoding a 28-kDa glycoprotein produced by the pars intermedia of the pituitary were isolated and characterized. Nucleotide sequencing demonstrated a precursor of the 28-kDa protein, which consisted of 231 amino acid residues, to be cleaved into a signal peptide (24 ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.87.11.4330

    authors: Ono M,Takayama Y,Rand-Weaver M,Sakata S,Yasunaga T,Noso T,Kawauchi H

    更新日期:1990-06-01 00:00:00

  • Oxygen-sensitive ribonucleoside triphosphate reductase is present in anaerobic Escherichia coli.

    abstract::Ribonucleoside diphosphate reductase from Escherichia coli and mammalian cells provides the deoxyribonucleoside triphosphates for DNA synthesis. The active enzyme contains a tyrosyl free radical whose formation requires oxygen. Earlier genetic evidence suggested that the enzyme is not required for anaerobic growth of ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.86.7.2147

    authors: Fontecave M,Eliasson R,Reichard P

    更新日期:1989-04-01 00:00:00

  • Accumulation of PNPLA3 on lipid droplets is the basis of associated hepatic steatosis.

    abstract::Fatty liver disease (FLD) is a disorder in which accumulation of triglycerides (TGs) in the liver can lead to inflammation, fibrosis, and cirrhosis. Previously, we identified a variant (I148M) in patatin-like phospholipase domain-containing protein 3 (PNPLA3) that is strongly associated with FLD, but the mechanistic b...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1901974116

    authors: BasuRay S,Wang Y,Smagris E,Cohen JC,Hobbs HH

    更新日期:2019-05-07 00:00:00

  • Effect of erythrocyte membrane on extracellular development of the erythrocytic cycle of Plasmodium falciparum.

    abstract::We have earlier reported conditions that support the axenic development in vitro of a complete asexual erythrocytic cycle of Plasmodium falciparum. Up to 30% of merozoites showed initial differentiation into trophic forms (rings) viable at 14 hr. However, only approximately 1% of the merozoites would develop further i...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.92.2.566

    authors: Williams JH,Gill GS,Trager W

    更新日期:1995-01-17 00:00:00

  • Innate immune receptor NOD2 mediates LGR5+ intestinal stem cell protection against ROS cytotoxicity via mitophagy stimulation.

    abstract::The nucleotide-binding oligomerization domain-containing protein 2 (NOD2) agonist muramyl dipeptide (MDP), a peptidoglycan motif common to all bacteria, supports leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5)+ intestinal stem cell (ISC) survival through NOD2 activation upon an otherwise lethal oxid...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1902788117

    authors: Levy A,Stedman A,Deutsch E,Donnadieu F,Virgin HW,Sansonetti PJ,Nigro G

    更新日期:2020-01-28 00:00:00

  • Long-range Pitx2c enhancer-promoter interactions prevent predisposition to atrial fibrillation.

    abstract::Genome-wide association studies found that increased risk for atrial fibrillation (AF), the most common human heart arrhythmia, is associated with noncoding sequence variants located in proximity to PITX2 Cardiomyocyte-specific epigenomic and comparative genomics uncovered 2 AF-associated enhancers neighboring PITX2 w...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1907418116

    authors: Zhang M,Hill MC,Kadow ZA,Suh JH,Tucker NR,Hall AW,Tran TT,Swinton PS,Leach JP,Margulies KB,Ellinor PT,Li N,Martin JF

    更新日期:2019-11-05 00:00:00

  • The final acylation step in taxol biosynthesis: cloning of the taxoid C13-side-chain N-benzoyltransferase from Taxus.

    abstract::The formation of several acyl groups and an amide group of Taxol is catalyzed by regioselective CoA thioester-dependent acyltransferases. Several full-length acyltransferase sequences, obtained from a cDNA library constructed from mRNA isolated from Taxus cuspidata cells induced for Taxol production with methyl jasmon...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.082115799

    authors: Walker K,Long R,Croteau R

    更新日期:2002-07-09 00:00:00

  • RANK is the intrinsic hematopoietic cell surface receptor that controls osteoclastogenesis and regulation of bone mass and calcium metabolism.

    abstract::We have generated RANK (receptor activator of NF-kappaB) nullizygous mice to determine the molecular genetic interactions between osteoprotegerin, osteoprotegerin ligand, and RANK during bone resorption and remodeling processes. RANK(-/-) mice lack osteoclasts and have a profound defect in bone resorption and remodeli...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.97.4.1566

    authors: Li J,Sarosi I,Yan XQ,Morony S,Capparelli C,Tan HL,McCabe S,Elliott R,Scully S,Van G,Kaufman S,Juan SC,Sun Y,Tarpley J,Martin L,Christensen K,McCabe J,Kostenuik P,Hsu H,Fletcher F,Dunstan CR,Lacey DL,Boyle WJ

    更新日期:2000-02-15 00:00:00

  • Brain structure in healthy adults is related to serum transferrin and the H63D polymorphism in the HFE gene.

    abstract::Control of iron homeostasis is essential for healthy central nervous system function: iron deficiency is associated with cognitive impairment, yet iron overload is thought to promote neurodegenerative diseases. Specific genetic markers have been previously identified that influence levels of transferrin, the protein t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1105543109

    authors: Jahanshad N,Kohannim O,Hibar DP,Stein JL,McMahon KL,de Zubicaray GI,Medland SE,Montgomery GW,Whitfield JB,Martin NG,Wright MJ,Toga AW,Thompson PM

    更新日期:2012-04-03 00:00:00

  • spoT-dependent accumulation of guanosine tetraphosphate in response to fatty acid starvation in Escherichia coli.

    abstract::We previously isolated a mutant of Escherichia coli that is preferentially affected in the synthesis of rRNA and has a mutation in the gene (accD) encoding a subunit of acetyl-CoA carboxylase. Using this mutant and other mutants of the pathway for fatty acid and phospholipid biosynthesis as well as cerulenin, a specif...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.90.23.11004

    authors: Seyfzadeh M,Keener J,Nomura M

    更新日期:1993-12-01 00:00:00

  • Microviscosity modulation during the cell cycle of neuroblastoma cells.

    abstract::Microviscosity (n) of the cell membrane lipid layer was determined in synchronized C1300 mouse neuroblastoma cells (clone Neuro-2A) by fluorescence polarization of 1,6-diphenystratum. The determined n value was maximal in mitosis, decreased markedly in the G1 phase, remained constant at a low level during the S phase,...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.74.10.4458

    authors: de Laat SW,van der Saag PT,Shinitzky M

    更新日期:1977-10-01 00:00:00

  • TAF250 is required for multiple developmental events in Drosophila.

    abstract::The TFIID transcription initiation complex is composed of TBP and multiple TAFs. Studies in unicellular systems indicate that TAF250 is required for progression through G(1)/S of the cell cycle and repression of apoptosis. Here we extend these in vivo studies by determining the developmental requirements for TAF250 in...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.97.3.1154

    authors: Wassarman DA,Aoyagi N,Pile LA,Schlag EM

    更新日期:2000-02-01 00:00:00

  • Relationship between the intra and intermolecular cross-links of collagen.

    abstract::Spontaneous cross-linking in vitro, during incubation of purified collagen in nonstriated and native type fibrils, was studied to elucidate the relationship between intra and intermolecular cross-links. In nonbanded fibrils, the aldol condensation products of two allysyl residues, previously shown to constitute the in...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.67.3.1307

    authors: Kang AH,Gross J

    更新日期:1970-11-01 00:00:00

  • Secreted MD-2 is a large polymeric protein that efficiently confers lipopolysaccharide sensitivity to Toll-like receptor 4.

    abstract::Toll-like receptor 4 (TLR4), the principal signaling receptor for lipopolysaccharide (LPS) in mammals, requires the binding of MD-2 to its extracellular domain for maximal responsiveness. MD-2 contains a leader sequence but lacks a transmembrane domain, and we asked whether it is secreted into the medium as an active ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.211445098

    authors: Visintin A,Mazzoni A,Spitzer JA,Segal DM

    更新日期:2001-10-09 00:00:00

  • Satellite tobacco ringspot virus RNA: A subset of the RNA sequence is sufficient for autolytic processing.

    abstract::The satellite RNA of tobacco ringspot virus depends upon tobacco ringspot virus for its replication and source of coat protein. The satellite RNA reduces virus accumulation and the severity of virus-induced symptoms. Repetitive sequence, dimeric, and higher forms of the satellite RNA are known to autolytically process...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.83.23.8859

    authors: Buzayan JM,Gerlach WL,Bruening G

    更新日期:1986-12-01 00:00:00