Abstract:
:Mucopolysaccharides have been isolated, fractionated, and characterized from the nuclei of cultured B16 mouse melanoma cells grown in the presence of (3-H)-glucosamine and (35-S)sulfate. Digestion of the nuclei with DNase followed by Pronase gave a mixture of complex carbohydrates from which the mucopolysaccharides were isolated by precipitation with cetylpyridinium chloride. After fractionation by differential salt extraction and chromatography on controlled pore glass bead columns, the components were identified by chemical and enzymatic methods. The major polysaccharide components were a family of high-molecular-weight chondroitin sulfates with different degrees of sulfation; a minor component has been characterized as heparan sulfate.
journal_name
Proc Natl Acad Sci U S Aauthors
Bhavanandan VP,Davidson EAdoi
10.1073/pnas.72.6.2032keywords:
subject
Has Abstractpub_date
1975-06-01 00:00:00pages
2032-6issue
6eissn
0027-8424issn
1091-6490journal_volume
72pub_type
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