Gene expression dynamics in the macrophage exhibit criticality.

abstract：:Ferulic, caffeic, chlorogenic, and ellagic acids, four naturally occurring plant phenols, inhibit the mutagenicity and cytotoxicity of (+/-)-7beta,8alpha-dihydroxy-9alpha, 10alpha-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (B[a]P 7,8-diol-9,10-epoxide-2), the only known ultimate carcinogenic metabolite of benzo[a]pyrene....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wood AW,Huang MT,Chang RL,Newmark HL,Lehr RE,Yagi H,Sayer JM,Jerina DM,Conney AH

更新日期：1982-09-01 00:00:00

abstract：:Sensitive optical signal detection can often be confounded by the presence of a significant background, and, as such, predetection background suppression is substantively important for weak signal detection. In this paper, we present a novel optical structure design, termed surface-wave-enabled darkfield aperture (SWE...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zheng G,Cui X,Yang C

更新日期：2010-05-18 00:00:00

abstract：:We have compared the process of prophage lambda induction with that of prophage Mu. According to the Campbell model, rescue of lambda DNA from the host DNA involves reversal of lambda integration such that the prophage DNA is excised from the host chromosome. We have monitored this event by locating the prophage DNA w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ljungquist E,Bukhari AI

更新日期：1977-08-01 00:00:00

abstract：:The nonlinearity of the Navier-Stokes equations makes predicting the flow of fluid around rapidly moving small bodies highly resistant to all approaches save careful experiments or brute force computation. Here, we show how a linearization of the Navier-Stokes equations captures the drag-determining features of the fl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roper M,Brenner MP

更新日期：2009-03-03 00:00:00

abstract：:Coagulation factor V is a high molecular weight plasma glycoprotein that participates as a cofactor in the conversion of prothrombin to thrombin by factor Xa. A phage lambda gt11 Hep G2 cell cDNA expression library was screened by using an affinity-purified antibody to human factor V, and 11 positive clones were isola...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kane WH,Davie EW

更新日期：1986-09-01 00:00:00

abstract：:We have demonstrated that the entire murine N-myc gene and the sequences necessary for its expression in human neuroblastoma cells are contained within a 7.4-kilobase murine genomic clone. The complete nucleotide sequence of this gene reveals a number of striking similarities and differences when compared to the relat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DePinho RA,Legouy E,Feldman LB,Kohl NE,Yancopoulos GD,Alt FW

更新日期：1986-03-01 00:00:00

abstract：:Both mammals and birds use the interaural time difference (ITD) for localization of sound in the horizontal plane. They may localize either real or phantom sound sources, when the signal consists of a narrow frequency band. This ambiguity does not occur with broadband signals. A plot of impulse rates or amplitude of e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peña JL,Konishi M

更新日期：2000-10-24 00:00:00

abstract：:The caspases are cysteine proteases that have been implicated in the execution of programmed cell death in organisms ranging from nematodes to humans. Many members of the Bcl-2 family, including Bcl-XL, are potent inhibitors of programmed cell death and inhibit activation of caspases in cells. Here, we report a direct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clem RJ,Cheng EH,Karp CL,Kirsch DG,Ueno K,Takahashi A,Kastan MB,Griffin DE,Earnshaw WC,Veliuona MA,Hardwick JM

更新日期：1998-01-20 00:00:00

abstract：:Addition of antisense oligonucleotides to cell cultures has been used to specifically inhibit gene expression. We have investigated the mechanism by which oligonucleotides enter living cells. These compounds are taken up by cells in a saturable, size-dependent manner compatible with receptor-mediated endocytosis. Poly...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Loke SL,Stein CA,Zhang XH,Mori K,Nakanishi M,Subasinghe C,Cohen JS,Neckers LM

更新日期：1989-05-01 00:00:00

abstract：:Telomere syndromes have their most common manifestation in lung disease that is recognized as idiopathic pulmonary fibrosis and emphysema. In both conditions, there is loss of alveolar integrity, but the underlying mechanisms are not known. We tested the capacity of alveolar epithelial and stromal cells from mice with...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alder JK,Barkauskas CE,Limjunyawong N,Stanley SE,Kembou F,Tuder RM,Hogan BL,Mitzner W,Armanios M

更新日期：2015-04-21 00:00:00

abstract：:The activities of cytosolic 3-hydroxy-3-methylglutaryl coenzyme A synthase [3-hydroxy-3-methylglutaryl-CoA acetoacetyl-CoA-lyase (CoA-acylating), EC 4.1.3.5] and microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase[mevalonate:NADP+ oxidoreductase (CoA-acylating), EC 1.1.1.34], two sequential enzymes in the chole...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balasubramaniam S,Goldstein JL,Brown MS

更新日期：1977-04-01 00:00:00

abstract：:Many bacterial pathogens of plants and animals have evolved a specialized protein-secretion system termed type III to deliver bacterial proteins into host cells. These proteins stimulate or interfere with host cellular functions for the pathogen's benefit. The Salmonella typhimurium pathogenicity island 1 encodes one ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kubori T,Sukhan A,Aizawa SI,Galán JE

更新日期：2000-08-29 00:00:00

abstract：:Adenosine 3':5'-cyclic monophosphate (cAMP) and guanosine 3':5'-cyclic monophosphate (cGMP) were localized in cells of rat kidney cortex by an immunocytochemical technique before and after perfusion with parathyroid hormone (PTH). In control tissues the cAMP antiserum detected approximately the same intensity of fluor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dousa TP,Barnes LD,Ong SH,Steiner AL

更新日期：1977-08-01 00:00:00

abstract：:The assembly of actin monomers into filaments and networks plays vital roles throughout eukaryotic biology, including intracellular transport, cell motility, cell division, determining cellular shape, and providing cells with mechanical strength. The regulation of actin assembly and modulation of filament mechanical p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kang H,Bradley MJ,McCullough BR,Pierre A,Grintsevich EE,Reisler E,De La Cruz EM

更新日期：2012-10-16 00:00:00

abstract：:We previously demonstrated that the primary region of factor IX and IXa responsible for saturable specific binding to bovine aortic endothelial cells resides in residues 3-11 at the amino terminus of factor IX. We also demonstrated that mutations of lysine to alanine at residue 5, factor IX K5A, or valine to lysine at...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheung WF,van den Born J,Kühn K,Kjellén L,Hudson BG,Stafford DW

更新日期：1996-10-01 00:00:00

abstract：:Absorbance changes reflecting the formation of a transient radical-pair state, P(F), were measured in reaction centers from Rhodopseudomonas sphaeroides under conditions that blocked electron transfer to a later carrier (a quinone, Q). The temperature dependence of the absorbance changes suggests that P(F) is an equil...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shuvalov VA,Parson WW

更新日期：1981-02-01 00:00:00

abstract：:Single strands of DNA serve, in rare instances, as promoters for transcription; duplex DNA promoters with individual strands that also have a promoter capacityfunction have not been described. We show that the nontranscribed strand of the Saccharomyces cerevisiae U6 snRNA gene directs transcription initiation factor I...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schroder O,Geiduschek EP,Kassavetis GA

更新日期：2003-02-04 00:00:00

abstract：:Although melanomas with mutant v-Raf murine sarcoma viral oncogene homolog B1 (BRAF) can now be effectively targeted, there is no molecular target for most melanomas expressing wild-type BRAF. Here, we show that the activation of Pleckstrin homology domain-interacting protein (PHIP), promotes melanoma metastasis, can ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Semir D,Nosrati M,Bezrookove V,Dar AA,Federman S,Bienvenu G,Venna S,Rangel J,Climent J,Meyer Tamgüney TM,Thummala S,Tong S,Leong SP,Haqq C,Billings P,Miller JR 3rd,Sagebiel RW,Debs R,Kashani-Sabet M

更新日期：2012-05-01 00:00:00

abstract：:Zea mays plasma membrane intrinsic proteins (ZmPIPs) fall into two groups, ZmPIP1s and ZmPIP2s, that exhibit different water channel activities when expressed in Xenopus oocytes. ZmPIP1s are inactive, whereas ZmPIP2s induce a marked increase in the membrane osmotic water permeability coefficient, P(f). We previously s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zelazny E,Borst JW,Muylaert M,Batoko H,Hemminga MA,Chaumont F

更新日期：2007-07-24 00:00:00

abstract：:A technique is described for isolation of plasmid DNA in closed and open circular double-stranded forms from bacterial cells, by use of ATP-dependent deoxyribonuclease purified from Micrococcus luteus. This DNase, acting only upon linear DNA molecules, degrades all bacterial chromosomal DNA extracted in the linear for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mukai T,Matsubara K,Takagi Y

更新日期：1973-10-01 00:00:00

abstract：:To assess the contribution to immunoglobulin heavy chain diversity made by recombination between variable region (VH) genes and joining region (JH) genes, we have determined the sequence of about 2000 nucleotides spanning the rearranged JH gene cluster associated with the VH gene expressed in plasmacytoma HPC76. The a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gough NM,Bernard O

更新日期：1981-01-01 00:00:00

abstract：:The study of specific trans-acting transcription factors in prokaryotes and lower eukaryotes has been greatly facilitated by genetic analysis of mutant strains deficient in such factors. We have developed such a system to study mammalian trans-acting factors that regulate the transcription of class II major histocompa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Calman AF,Peterlin BM

更新日期：1988-12-01 00:00:00

abstract：:The postdevelopmental basis of cellular identity and the unique cellular output of a particular neuron type are of particular interest in the nervous system because a detailed understanding of circuits responsible for complex processes in the brain is impeded by the often ambiguous classification of neurons in these c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schulz DJ,Goaillard JM,Marder EE

更新日期：2007-08-07 00:00:00

abstract：:The HER2 gene encodes a cell-surface glycoprotein with extensive homology to the epidermal growth factor receptor. Recently it was found to be amplified in about 30% of primary human breast malignancies. In experiments designed to assess the role of the HER2 gene in oncogenesis, we found that overexpression of unalter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hudziak RM,Schlessinger J,Ullrich A

更新日期：1987-10-01 00:00:00

abstract：:A joint analysis of all-atom molecular dynamics (MD) calculations and picosecond time-resolved x-ray structures was performed to gain single-molecule insights into mechanisms of protein function. Ensemble-averaged MD simulations of the L29F mutant of myoglobin after ligand dissociation reproduce the direction, amplitu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hummer G,Schotte F,Anfinrud PA

更新日期：2004-10-26 00:00:00

abstract：:Plant cutin monomers trigger, and glucose suppresses, the expression of the cutinase gene of pathogenic fungi. To identify the cutinase promoter region responsible for induction by the unique plant components, a promoter analysis was done with transformants. Plasmids were constructed that contained (i) the 5' flanking...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bajar A,Podila GK,Kolattukudy PE

更新日期：1991-09-15 00:00:00

abstract：:An ability to safely harness the powerful regenerative potential of adult stem cells for clinical applications is critically dependent on a comprehensive understanding of the underlying mechanisms regulating their activity. Epithelial organoid cultures accurately recapitulate many features of in vivo stem cell-driven ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murakami K,Terakado Y,Saito K,Jomen Y,Takeda H,Oshima M,Barker N

更新日期：2021-01-26 00:00:00

abstract：:DNA polymerases replicate DNA by adding nucleotides to a growing primer strand while avoiding frameshift and point mutations. Here we present a series of up to six successive replication events that were obtained by extension of a primed template directly in a crystal of the thermostable Bacillus DNA polymerase I. The...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Johnson SJ,Taylor JS,Beese LS

更新日期：2003-04-01 00:00:00

abstract：:Obesity is a major public health problem and is often associated with type 2 diabetes mellitus, cardiovascular disease, and metabolic syndrome. Leptin is the crucial adipostatic hormone that controls food intake and body weight through the activation of specific leptin receptors (OB-R) in the hypothalamic arcuate nucl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Couturier C,Sarkis C,Séron K,Belouzard S,Chen P,Lenain A,Corset L,Dam J,Vauthier V,Dubart A,Mallet J,Froguel P,Rouillé Y,Jockers R

更新日期：2007-12-04 00:00:00

abstract：:By using a simplified model of small open liquid-like clusters with surface effects, in the gas phase, it is shown how the statistical thermodynamics of small systems can be extended to include metastable supersaturated gaseous states not too far from the gas-liquid equilibrium transition point. To accomplish this, on...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hill TL,Chamberlin RV

更新日期：1998-10-27 00:00:00