Increased expression of the putative growth factor receptor p185HER2 causes transformation and tumorigenesis of NIH 3T3 cells.

abstract：:We have made direct, quantitative measurements of the lateral motion and age-dependent distribution of acetylcholine receptors (AChR) on the surface of rat myotubes in primary culture. AChR were fluorescently marked with tetramethylrhodamine-labeled alpha-bungarotoxin and AChR lateral motion was measured by the fluore...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Axelrod D,Ravdin P,Koppel DE,Schlessinger J,Webb WW,Elson EL,Podleski TR

更新日期：1976-12-01 00:00:00

abstract：:Effects of opiate receptor antagonists on centrally mediated cardiovascular responses to clonidine and beta-endorphin were studied in urethane-anesthetized spontaneously hypertensive Okamoto-Aoki rats (SHR), normotensive Sprague-Dawley rats, and Sprague-Dawley rats made hypertensive with deoxycorticosterone pivalate/s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mosqueda-Garcia R,Kunos G

更新日期：1987-12-01 00:00:00

abstract：:Selective lodgement or homing of transplanted hemopoietic stem cells in the recipient's bone marrow (BM) is a critical step in the establishment of long-term hemopoiesis after BM transplantation. However, despite its biologic and clinical significance, little is understood about the process of homing. In the present s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Papayannopoulou T,Craddock C,Nakamoto B,Priestley GV,Wolf NS

更新日期：1995-10-10 00:00:00

abstract：:We present a proposition, the "poly(L-alanine) hypothesis," which asserts that the native backbone geometry for any polypeptide or protein of M residues has a closely mimicking, mechanically stable, image in poly(L-alanine) of the same number of residues. Using a molecular mechanics force field to represent the releva...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Head-Gordon T,Stillinger FH,Wright MH,Gay DM

更新日期：1992-12-01 00:00:00

abstract：:In polyandrous species, fathers benefit from attracting greater maternal investment toward their offspring at the expense of the offspring of other males, while mothers should usually allocate resources equally among offspring. This conflict can lead to an evolutionary arms race between the sexes, manifested through a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raunsgard A,Opedal ØH,Ekrem RK,Wright J,Bolstad GH,Armbruster WS,Pélabon C

更新日期：2018-11-06 00:00:00

abstract：:We have isolated three classes of monoclonal antibodies against Drosophila cell-surface antigens that are expressed with positional specificity in imaginal discs. Comparison of immunofluorescence patterns with the wing-disc fate map reveals that expression of the antigens is not directly related to the specific type o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brower DL,Wilcox M,Piovant M,Smith RJ,Reger LA

更新日期：1984-12-01 00:00:00

abstract：:The Belgrade (b) rat has an autosomal recessively inherited, microcytic, hypochromic anemia associated with abnormal reticulocyte iron uptake and gastrointestinal iron absorption. The b reticulocyte defect appears to be failure of iron transport out of endosomes within the transferrin cycle. Aspects of this phenotype ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fleming MD,Romano MA,Su MA,Garrick LM,Garrick MD,Andrews NC

更新日期：1998-02-03 00:00:00

abstract：:The hetR gene plays a very important role in cell differentiation of heterocystous cyanobacteria. To understand the mechanism of the hetR gene product in regulation of heterocyst differentiation, the recombinant HetR protein (rHetR) was overproduced in Escherichia coli. Purified rHetR was unstable and degraded easily ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou R,Wei X,Jiang N,Li H,Dong Y,Hsi KL,Zhao J

更新日期：1998-04-28 00:00:00

abstract：:A hypothetical gene with similarity to the ispD gene of Escherichia coli was cloned from Arabidopsis thaliana cDNA. The ORF of 909 bp specifies a protein of 302 amino acid residues. The cognate chromosomal gene consists of 2,071 bp and comprises 11 introns with a size range of 78-202 bp. A fragment comprising amino ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rohdich F,Wungsintaweekul J,Eisenreich W,Richter G,Schuhr CA,Hecht S,Zenk MH,Bacher A

更新日期：2000-06-06 00:00:00

abstract：:Tubular membrane structures are widespread in eukaryotic cells, but the mechanisms underlying their formation and stability are not well understood. Previous work has focused on tube extrusion from cells and model membranes under the application of external forces. Here, we present novel membrane/polymer systems, wher...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Y,Lipowsky R,Dimova R

更新日期：2011-03-22 00:00:00

abstract：:An exact treatment of adsorption from a one-dimensional lattice gas is used to eliminate and correct a well-known inconsistency in the Brunauer-Emmett-Teller (B.E.T.) equation-namely, Gibbs excess adsorption is not taken into account and the Gibbs integral diverges at the transition point. However, neither model shoul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hill TL

更新日期：1996-12-10 00:00:00

abstract：:Modulation of cholesterol absorption in the intestine, the primary site of dietary cholesterol uptake in humans, can have profound clinical implications. We have undertaken a reverse genetic approach by disrupting putative cholesterol processing genes in zebrafish larvae by using morpholino (MO) antisense oligonucleot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smart EJ,De Rose RA,Farber SA

更新日期：2004-03-09 00:00:00

abstract：:Array technologies have made it straightforward to monitor simultaneously the expression pattern of thousands of genes. The challenge now is to interpret such massive data sets. The first step is to extract the fundamental patterns of gene expression inherent in the data. This paper describes the application of self-o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tamayo P,Slonim D,Mesirov J,Zhu Q,Kitareewan S,Dmitrovsky E,Lander ES,Golub TR

更新日期：1999-03-16 00:00:00

abstract：:Tomato flavor is dependent upon a complex mixture of volatiles including multiple acetate esters. Red-fruited species of the tomato clade accumulate a relatively low content of acetate esters in comparison with the green-fruited species. We show that the difference in volatile ester content between the red- and green-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goulet C,Mageroy MH,Lam NB,Floystad A,Tieman DM,Klee HJ

更新日期：2012-11-13 00:00:00

abstract：:Information concerning the dynamics of river meandering is embedded in their planforms. Here, we focus on how bend skewing varies with increasing sinuosity, and how flow direction is embedded in bend skewing. It has often been thought that upstream-skewed bends are dominant within a sufficiently long reach. These bend...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo X,Chen D,Parker G

更新日期：2019-11-19 00:00:00

abstract：:An isoform of the mammalian renal type II Na/Pi-cotransporter is described. Homology of this isoform to described mammalian and nonmammalian type II cotransporters is between 57 and 75%. Based on major diversities at the C terminus, the new isoform is designed as type IIb Na/Pi-cotransporter. Na/Pi-cotransport mediate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hilfiker H,Hattenhauer O,Traebert M,Forster I,Murer H,Biber J

更新日期：1998-11-24 00:00:00

abstract：:The oligodendrocyte (Od), a glial cell that produces myelin in the central nervous system, may be a target for autoreactive T cells in autoimmune demyelinating processes, although not expressing major histocompatibility complex (MHC) products. To analyze Od-T-cell interactions, we selected from normal SJL/J mouse sple...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jewtoukoff V,Lebar R,Bach MA

更新日期：1989-04-01 00:00:00

abstract：:Coagulation factor V is a high molecular weight plasma glycoprotein that participates as a cofactor in the conversion of prothrombin to thrombin by factor Xa. A phage lambda gt11 Hep G2 cell cDNA expression library was screened by using an affinity-purified antibody to human factor V, and 11 positive clones were isola...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kane WH,Davie EW

更新日期：1986-09-01 00:00:00

abstract：:Glial cells are traditionally regarded as elements for structural support and ionic homeostasis, but have recently attracted attention as putative integral elements of the machinery involved in synaptic transmission and plasticity. Here, we demonstrate that calcium-binding protein S100B, which is synthesized in consid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nishiyama H,Knopfel T,Endo S,Itohara S

更新日期：2002-03-19 00:00:00

abstract：:Recent studies have suggested that Paleozoic hyperoxia enabled animal gigantism, and the subsequent hypoxia drove a reduction in animal size. This evolutionary hypothesis depends on the argument that gas exchange in many invertebrates and skin-breathing vertebrates becomes compromised at large sizes because of distanc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaiser A,Klok CJ,Socha JJ,Lee WK,Quinlan MC,Harrison JF

更新日期：2007-08-07 00:00:00

abstract：:The mature optic nerve cannot regenerate when injured, leaving victims of traumatic nerve damage or diseases such as glaucoma with irreversible visual losses. Recent studies have identified ways to stimulate retinal ganglion cells to regenerate axons part-way through the optic nerve, but it remains unknown whether mat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： de Lima S,Koriyama Y,Kurimoto T,Oliveira JT,Yin Y,Li Y,Gilbert HY,Fagiolini M,Martinez AM,Benowitz L

更新日期：2012-06-05 00:00:00

abstract：:When we grasp and manipulate an object, populations of tactile nerve fibers become activated and convey information about the shape, size, and texture of the object and its motion across the skin. The response properties of tactile fibers have been extensively characterized in single-unit recordings, yielding importan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saal HP,Delhaye BP,Rayhaun BC,Bensmaia SJ

更新日期：2017-07-11 00:00:00

abstract：:The kinetics of refolding of Escherichia coli dihydrofolate reductase (EC 1.5.1.3) have been examined upon dilution of unfolded enzyme in 4.5 M urea to 1.29 M urea in 0.02 M phosphate buffer (pH 7.2) at 10 degrees C. Changes in the intrinsic protein fluorescence on refolding are characterized by four phases. Based on ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frieden C

更新日期：1990-06-01 00:00:00

abstract：:We have obtained long-term cultures of differentiated proliferating follicular cells from normal adult human thyroid glands. In vitro growth of such human cells has been sustained by a modified F-12 medium, supplemented with bovine hypothalamus and pituitary extracts and no added thyrotropin. Cultures have been expand...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Curcio F,Ambesi-Impiombato FS,Perrella G,Coon HG

更新日期：1994-09-13 00:00:00

abstract：:Microarrays have enabled the determination of how thousands of genes are expressed to coordinate function within single organisms. Yet applications to natural or engineered communities where different organisms interact to produce complex properties are hampered by theoretical and technological limitations. Here we de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marcelino LA,Backman V,Donaldson A,Steadman C,Thompson JR,Preheim SP,Lien C,Lim E,Veneziano D,Polz MF

更新日期：2006-09-12 00:00:00

abstract：:This work describes a multifunctional phage lambda expression vector system, lambda YES, designed to facilitate gene isolation from eukaryotes by complementation of Escherichia coli and Saccharomyces cerevisiae mutations. lambda YES vectors have a selection for cDNA inserts using an oligo adaptor strategy and are capa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elledge SJ,Mulligan JT,Ramer SW,Spottswood M,Davis RW

更新日期：1991-03-01 00:00:00

abstract：:The excision and reintegration of transposable elements (TEs) restructure their host genomes, generating cellular diversity involved in evolution, development, and the etiology of human diseases. Our current knowledge of TE behavior primarily results from bulk techniques that generate time and cell ensemble averages, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim NH,Lee G,Sherer NA,Martini KM,Goldenfeld N,Kuhlman TE

更新日期：2016-06-28 00:00:00

abstract：:Deinococcus radiodurans is among a small number of bacterial species that are extremely resistant to ionizing radiation, UV light, toxic chemicals, and desiccation. We measured proteome oxidation (i.e., protein carbonylation, PC) in D. radiodurans as well as in standard and evolved resistant strains of Escherichia col...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krisko A,Radman M

更新日期：2010-08-10 00:00:00

abstract：:High molecular weight RNA (35S) isolated from avian myeloblastosis virus directs the cell-free synthesis of two prominent polypeptides of 180,000 and 76,000 molecular weight. The latter polypeptide has previously been identified as the precursor to the group-specific antigens of the virus ("gag" proteins) [Vogt, V. M....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paterson BM,Marciani DJ,Papas TS

更新日期：1977-11-01 00:00:00

abstract：:Translation of injected mRNA in oocytes of Xenopus laevis has been used as a highly sensitive and quantitative assay for interferon mRNA. Injection into oocytes of polyadenylylated RNA extracted from poly(I).poly(C)-induced human diploid fibroblasts (FS-4) leads to the synthesis of biologically active human fibroblast...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sehgal PB,Dobberstein B,Tamm I

更新日期：1977-08-01 00:00:00