Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations.

abstract：:The α-proteobacterium Sinorhizobium meliloti establishes a chronic intracellular infection during the symbiosis with its legume hosts. Within specialized host cells, S. meliloti differentiates into highly polyploid, enlarged nitrogen-fixing bacteroids. This differentiation is driven by host cells through the productio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Penterman J,Abo RP,De Nisco NJ,Arnold MF,Longhi R,Zanda M,Walker GC

更新日期：2014-03-04 00:00:00

abstract：:To investigate the contribution of interleukin-4 (IL-4) to airway inflammation in vivo and to explore directly its relationship to airway reactivity, we created transgenic mice in which the murine cDNA for IL-4 was regulated by the rat Clara cell 10 protein promoter. Expression was detected only in the lung and not in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rankin JA,Picarella DE,Geba GP,Temann UA,Prasad B,DiCosmo B,Tarallo A,Stripp B,Whitsett J,Flavell RA

更新日期：1996-07-23 00:00:00

abstract：:The mammalian hair follicle unit consists of a central follicle and a series of associated structures: sebaceous glands, arrector pili muscles, Merkel cells, and sensory nerve endings. The architecture of this multicellular structure is highly polarized with respect to the body axes. Previous work has implicated Frizz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang H,Nathans J

更新日期：2013-03-05 00:00:00

abstract：:The highly productive coastal Mediterranean fishery off the Nile River delta collapsed after the completion of the Aswan High Dam in 1965. But the fishery has been recovering dramatically since the mid-1980s, coincident with large increases in fertilizer application and sewage discharge in Egypt. We use stable isotope...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oczkowski AJ,Nixon SW,Granger SL,El-Sayed AF,McKinney RA

更新日期：2009-02-03 00:00:00

abstract：:The D1 reaction center protein of photosystem II (PSII) is subject to light-induced damage. Degradation of damaged D1 and its replacement by nascent D1 are at the heart of a PSII repair cycle, without which photosynthesis is inhibited. In mature plant chloroplasts, light stimulates the recruitment of ribosomes specifi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chotewutmontri P,Barkan A

更新日期：2020-09-01 00:00:00

abstract：:In eukaryotes, transfer RNAs (tRNAs) are transcribed in the nucleus yet function in the cytoplasm; thus, tRNA movement within the cell was believed to be unidirectional--from the nucleus to the cytoplasm. It is now known that mature tRNAs also move in a retrograde direction from the cytoplasm to the nucleus via retrog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kramer EB,Hopper AK

更新日期：2013-12-24 00:00:00

abstract：:Climate change is expected to cause shifts in the composition of tropical montane forests towards increased relative abundances of species whose ranges were previously centered at lower, hotter elevations. To investigate this process of "thermophilization," we analyzed patterns of compositional change over the last de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Duque A,Stevenson PR,Feeley KJ

更新日期：2015-08-25 00:00:00

abstract：:L-Fucose and D-galactose in low concentrations (0.27 or 2.7 mM) inhibited the induction of active Na+ channels in mouse and human neuroblastoma cells when the monosaccharides were added to the culture medium for 4 days with the inducing agent dimethyl sulfoxide. Active Na+ ionophores were determined by measurement of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giovanni MY,Kessel D,Glick MC

更新日期：1981-02-01 00:00:00

abstract：:Among flowering plants, females often have little control over the genetic relatedness of pollen deposited on stigmas. Thus, postpollination processes are of primary importance for mate discrimination. The ability to screen and select among male gametes during pollen tube growth within the female tissues of the stigma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams JH Jr,Friedman WE,Arnold ML

更新日期：1999-08-03 00:00:00

abstract：:The Hippo-YAP/TAZ signaling pathway plays a pivotal role in growth control during development and regeneration and its dysregulation is widely implicated in various cancers. To further understand the cellular and molecular mechanisms underlying Hippo signaling regulation, we have found that activities of core Hippo si...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim W,Cho YS,Wang X,Park O,Ma X,Kim H,Gan W,Jho EH,Cha B,Jeung YJ,Zhang L,Gao B,Wei W,Jiang J,Chung KS,Yang Y

更新日期：2019-05-07 00:00:00

abstract：:The importance of founder events in promoting evolutionary changes on islands has been a subject of long-running controversy. Resolution of this debate has been hindered by a lack of empirical evidence from naturally founded island populations. Here we undertake a genetic analysis of a series of historically documente...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clegg SM,Degnan SM,Kikkawa J,Moritz C,Estoup A,Owens IP

更新日期：2002-06-11 00:00:00

abstract：:Chondrules may have played a critical role in the earliest stages of planet formation by mediating the accumulation of dust into planetesimals. However, the origin of chondrules and their significance for planetesimal accretion remain enigmatic. Here, we show that chondrules and matrix in the carbonaceous chondrite Al...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Budde G,Kleine T,Kruijer TS,Burkhardt C,Metzler K

更新日期：2016-03-15 00:00:00

abstract：:Phage phi 29 DNA cannot be phosphorylated with polynucleotide kinase and [gamma-32P]ATP because of the presence of a viral protein covalently linked to the 5' termini. The 5' ends can, however, be made susceptible to phosphorylation by treatment with alkali and alkaline phosphatase. Restriction fragments Hpa II C and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Escarmís C,Salas M

更新日期：1981-03-01 00:00:00

abstract：:As a consequence of recent developments in molecular phylogenomics, all extant orders of placental mammals have been grouped into 3 lineages: Afrotheria, Xenarthra, and Boreotheria, which originated in Africa, South America, and Laurasia, respectively. Despite this advancement, the order of divergence of these 3 linea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nishihara H,Maruyama S,Okada N

更新日期：2009-03-31 00:00:00

abstract：:The wetting of microstructured surfaces is studied both experimentally and theoretically. Even relatively simple surface topographies such as grooves with rectangular cross section exhibit a large variety of different wetting morphologies as observed by atomic force microscopy. This polymorphism arises from liquid wed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seemann R,Brinkmann M,Kramer EJ,Lange FF,Lipowsky R

更新日期：2005-02-08 00:00:00

abstract：:Telomeric repeat-containing RNA (referred to as TERRA), a noncoding RNA molecule, has recently been found in mammalian cells. The detailed structural features and function of the TERRA RNA at human chromosome ends remain unclear, although this RNA molecule may be a key component of the telomere machinery. In the prese...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu Y,Suzuki Y,Ito K,Komiyama M

更新日期：2010-08-17 00:00:00

abstract：:Epithelial tissues mechanically deform the surrounding extracellular matrix during embryonic development, wound repair, and tumor invasion. Ex vivo measurements of such multicellular tractions within three-dimensional (3D) biomaterials could elucidate collective dissemination during disease progression and enable prec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leggett SE,Patel M,Valentin TM,Gamboa L,Khoo AS,Williams EK,Franck C,Wong IY

更新日期：2020-03-17 00:00:00

abstract：:Tooth decay (dental caries) is a widespread human disease caused by microbial biofilms. Streptococcus mutans, a biofilm-former, has been consistently associated with severe childhood caries; however, how this bacterium is spatially organized with other microorganisms in the oral cavity to promote disease remains unkno...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim D,Barraza JP,Arthur RA,Hara A,Lewis K,Liu Y,Scisci EL,Hajishengallis E,Whiteley M,Koo H

更新日期：2020-06-02 00:00:00

abstract：:Stroke patients with small central nervous system infarcts often demonstrate an acute dysexecutive syndrome characterized by difficulty with attention, concentration, and processing speed, independent of lesion size or location. We use magnetoencephalography (MEG) to show that disruption of network dynamics may be res...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marsh EB,Brodbeck C,Llinas RH,Mallick D,Kulasingham JP,Simon JZ,Llinás RR

更新日期：2020-12-29 00:00:00

abstract：:Soil systems are being increasingly exposed to the interactive effects of biological invasions and climate change, with rising temperatures expected to benefit alien over indigenous species. We assessed this expectation for an important soil-dwelling group, the springtails, by determining whether alien species show br...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Janion-Scheepers C,Phillips L,Sgrò CM,Duffy GA,Hallas R,Chown SL

更新日期：2018-01-02 00:00:00

abstract：:The 1.1 Å, ultrahigh resolution neutron structure of hydrogen/deuterium (H/D) exchanged crambin is reported. Two hundred ninety-nine out of 315, or 94.9%, of the hydrogen atom positions in the protein have been experimentally derived and resolved through nuclear density maps. A number of unconventional interactions ar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen JC,Hanson BL,Fisher SZ,Langan P,Kovalevsky AY

更新日期：2012-09-18 00:00:00

abstract：:Unlike other cation channels, each subunit of most transient receptor potential (TRP) channels has an additional TRP-domain helix with an invariant tryptophan immediately trailing the gate-bearing S6. Recent cryo-electron microscopy of TRP vanilloid subfamily, member 1 structures revealed that this domain is a five-tu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teng J,Loukin SH,Anishkin A,Kung C

更新日期：2015-03-17 00:00:00

abstract：:Kinesin-73 cDNA was shown to encode a kinesin heavy chain protein that contains an N-terminal motor domain and a long central region that lacks extensive coiled-coils. The amino acid sequence of the motor domain of kinesin-73 protein is most closely related to the motor domains of Caenorhabditis elegans unc-104 and mo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li HP,Liu ZM,Nirenberg M

更新日期：1997-02-18 00:00:00

abstract：:The idea that the first living systems on earth were based on self-replicating RNA molecules has recently become popular as a result of the discovery of ribozymes. However, there are several major problems associated with the prebiotic synthesis of ribonucleotides. In addition, there is the newly recognized problem of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Joyce GF,Schwartz AW,Miller SL,Orgel LE

更新日期：1987-07-01 00:00:00

abstract：:Plasma samples exhibiting alloalbuminemia on electrophoresis at pH 8.6 were requested from clinical laboratories throughout Sweden. Nine variants, each representing a different single point mutation, were found in 100 apparently unrelated Swedes. The overall prevalence of alloalbuminemia was estimated at 1:1700. Mutat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carlson J,Sakamoto Y,Laurell CB,Madison J,Watkins S,Putnam FW

更新日期：1992-09-01 00:00:00

abstract：:Poly(A)+ mRNA was isolated from the brains of C57BL/6J (B6), DBA/2J (D2), and F1 hybrid mice (B6 X D2) of 16-17 days of age. The yield of polysomal RNA, both poly(A)+ and poly(A)-, from the three strains of mice was comparable. When translated in vitro in a reticulocyte lysate system, the mRNA preparations had the sam...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miskimins R,Ebato H,Seyfried TN,Yu RK

更新日期：1986-03-01 00:00:00

abstract：:To better understand how the relatively flat antigen-combining sites of antibodies interact with the concave shaped substrate-binding clefts of proteases, we determined the structures of two antibodies in complex with the trypsin-like hepatocyte growth-factor activator (HGFA). The two inhibitory antibodies, Ab58 and A...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu Y,Eigenbrot C,Liang WC,Stawicki S,Shia S,Fan B,Ganesan R,Lipari MT,Kirchhofer D

更新日期：2007-12-11 00:00:00

abstract：:Hox genes encode transcription factors that control spatial patterning during embryogenesis. To date, downstream targets of Hox genes have proven difficult to identify. Here, we describe studies designed to identify target genes under the control of the murine transcription factor Hoxc8. We used a mouse 16,463 gene ol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lei H,Wang H,Juan AH,Ruddle FH

更新日期：2005-02-15 00:00:00

abstract：:Cellular metabolism alters patterns of gene expression through a variety of mechanisms, including alterations in histone modifications and transcription factor activity. Nicotinamide adenine dinucleotide (NAD)-dependent proteins such as poly(ADP ribose) polymerases (PARPs) and sirtuin deacetylases play important roles...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang J,Zhang B,Heath H,Galjart N,Wang X,Milbrandt J

更新日期：2010-12-14 00:00:00

abstract：:Optical imaging of individual vesicle exocytosis is providing new insights into the mechanism and regulation of secretion by cells. To study calcium-triggered secretion from astrocytes, we used acridine orange (AO) to label vesicles. Although AO is often used for imaging exocytosis, we found that imaging vesicles labe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jaiswal JK,Fix M,Takano T,Nedergaard M,Simon SM

更新日期：2007-08-28 00:00:00