Real-time transposable element activity in individual live cells.

abstract：:Electron micrographs are shown of the first component of human complement (C1) which has been crosslinked with a water-soluble carbodiimide to prevent dissociation into its C1q and C1r2C1s2 subunits. Two projections of the crosslinked molecule are seen in the electron micrographs, which are called "top" and "profile."...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Strang CJ,Siegel RC,Phillips ML,Poon PH,Schumaker VN

更新日期：1982-01-01 00:00:00

abstract：:Understanding how institutional changes within academia may affect the overall potential of science requires a better quantitative representation of how careers evolve over time. Because knowledge spillovers, cumulative advantage, competition, and collaboration are distinctive features of the academic profession, both...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Petersen AM,Riccaboni M,Stanley HE,Pammolli F

更新日期：2012-04-03 00:00:00

abstract：:An expression in the exterior algebra of a Peano space yielding Pappus' theorem was originally given by Doubilet, Rota, and Stein [Doubilet, P., Rota, G.-C. & Stein, J. (1974) Stud. Appl. Math. 8, 185-216]. Motivated by an identity of Rota, I give an identity in a Grassmann-Cayley algebra of step 3, involving joins an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hawrylycz M

更新日期：1994-04-12 00:00:00

abstract：:Advances in vaccine technology over the past two centuries have facilitated far-reaching impact in the control of many infections, and today's emerging vaccines could likewise open new opportunities in the control of several diseases. Here we consider the potential, population-level effects of a particular class of em...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arinaminpathy N,Lavine JS,Grenfell BT

更新日期：2012-12-04 00:00:00

abstract：:Analysis, by the recovery of specific genetic "markers," of the effects of DNase I, physical shear, and temperature shock on DNA-cell complexes demonstrates that sequential attachment of both ends of bacteriophage SP82G DNA to Bacillus subtilis precedes entry of the DNA molecule into the cell, and that each attachment...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams GL,Green DM

更新日期：1972-06-01 00:00:00

abstract：:The human major histocompatibility complex includes approximately 14 class II HLA genes within the HLA-D region, most of which exist in multiple allelic forms. One of these genes, the DQ3.2 beta gene, accounts for the well-documented association of HLA-DR4 with insulin-dependent diabetes mellitus and is the single all...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kwok WW,Lotshaw C,Milner EC,Knitter-Jack N,Nepom GT

更新日期：1989-02-01 00:00:00

abstract：:Enteritis caused by Clostridium difficile toxin (Tx) is a nosocomial disease of increasing clinical concern, but the local mediators of C. difficile TxA inflammation are unknown. The potent vasodilator calcitonin gene-related peptide mediates neurogenic inflammation via the calcitonin receptor-like receptor (CLR). Her...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bhargava A,Clifton MS,Mhaske P,Liao M,Pothoulakis C,Leeman SE,Grady EF

更新日期：2013-01-08 00:00:00

abstract：:Administration of beta-sitosterol (42 mg/kg per day) for 3 weeks to 8-month-old male LXRbeta-/- mice resulted in the death of motor neurons in the lumbar region of the spinal cord and loss of tyrosine hydroxylase-positive dopaminergic neurons in the substantia nigra. In mice at 5 months of age, beta-sitosterol had no ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim HJ,Fan X,Gabbi C,Yakimchuk K,Parini P,Warner M,Gustafsson JA

更新日期：2008-02-12 00:00:00

abstract：:Polyamides containing N-methylimidazole (Im) and N-methylpyrrole (Py) amino acids can be combined in antiparallel side-by-side dimeric complexes for sequence-specific recognition in the minor groove of DNA. Six polyamides containing three to eight rings bind DNA sites 5-10 bp in length, respectively. Quantitative DNas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kelly JJ,Baird EE,Dervan PB

更新日期：1996-07-09 00:00:00

abstract：:The circadian rhythms of melatonin and body temperature are set to an earlier hour in women than in men, even when the women and men maintain nearly identical and consistent bedtimes and wake times. Moreover, women tend to wake up earlier than men and exhibit a greater preference for morning activities than men. Altho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Duffy JF,Cain SW,Chang AM,Phillips AJ,Münch MY,Gronfier C,Wyatt JK,Dijk DJ,Wright KP Jr,Czeisler CA

更新日期：2011-09-13 00:00:00

abstract：:In target tissue extracts, heat shock protein hsp90 has been found associated to all unliganded steroid receptors. Modulation of important functions of these receptors, including prevention of DNA binding and optimization of transcriptional activity, has been attributed to hsp90. However no unequivocal in vivo demonst...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kang KI,Devin J,Cadepond F,Jibard N,Guiochon-Mantel A,Baulieu EE,Catelli MG

更新日期：1994-01-04 00:00:00

abstract：:Queuosine (Q) is a complex tRNA modification widespread in eukaryotes and bacteria that contributes to the efficiency and accuracy of protein synthesis. Eukaryotes are not capable of Q synthesis and rely on salvage of the queuine base (q) as a Q precursor. While many bacteria are capable of Q de novo synthesis, salvag...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yuan Y,Zallot R,Grove TL,Payan DJ,Martin-Verstraete I,Šepić S,Balamkundu S,Neelakandan R,Gadi VK,Liu CF,Swairjo MA,Dedon PC,Almo SC,Gerlt JA,de Crécy-Lagard V

更新日期：2019-09-17 00:00:00

abstract：:Sponges (Porifera), the simplest and earliest multicellular organisms, are thought to have evolved from their unicellular ancestors about 1 billion years ago by developing cell-recognition and adhesion mechanisms to discriminate against "non-self." Consequently, they are used as models for investigating recognition ph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haseley SR,Vermeer HJ,Kamerling JP,Vliegenthart JF

更新日期：2001-07-31 00:00:00

abstract：:Interleukin 3 (IL-3) stimulates the proliferation and differentiation of various haematopoietic progenitor cells. Recently, IL-3 and other cytokines were reported to exert a neurotrophic activity and to be associated with neurological disorders, suggesting their complex role in the central nervous system. We now show ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chavany C,Vicario-Abejón C,Miller G,Jendoubi M

更新日期：1998-09-15 00:00:00

abstract：:Mixing genomes of different species by hybridization can disrupt species-specific genetic interactions that were adapted and fixed within each species population. Such disruption can predispose the hybrids to abnormalities and disease that decrease the overall fitness of the hybrids and is therefore named as hybrid in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu Y,Sandoval A,Voss S,Lai Z,Kneitz S,Boswell W,Boswell M,Savage M,Walter C,Warren W,Schartl M,Walter R

更新日期：2020-11-24 00:00:00

abstract：:Experimentally, protein engineering and phi-value analysis is the method of choice to characterize the structure in folding transition state ensemble (TSE) of any protein. Combining experimental phi values and computer simulations has led to a deeper understanding of how proteins fold. In this report, we construct the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li L,Shakhnovich EI

更新日期：2001-11-06 00:00:00

abstract：:A chimeric transforming growth factor beta (TGF-beta) molecule has been synthesized to map the amino acids responsible for the substantially greater activity of TGF-beta 1 than TGF-beta 2 on growth and migration of endothelial cells. This chimera consists of a dimer of a monomeric unit composed of amino acids 1-39 of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qian SW,Burmester JK,Merwin JR,Madri JA,Sporn MB,Roberts AB

更新日期：1992-07-15 00:00:00

abstract：:Nuclear proteins contain specific regions that are required for entry into the nucleus. Using ligand blotting, we have shown that a 67-kDa yeast nuclear envelope protein (p67) recognizes synthetic peptides containing the yeast histone H2B or simian virus 40 large tumor antigen nuclear localization sequence. Both free ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee WC,Mélèse T

更新日期：1989-11-01 00:00:00

abstract：:Spontaneous liposome formation is predicted in binary mixtures of fluid phase phospholipids and poly(n)ethylene oxide (PEO)-bearing lipids by using single chain mean field theory. The range of stability of the spontaneous liposomes is determined as a function of percentage of PEO-conjugated lipids and polymer molecula...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Szleifer I,Gerasimov OV,Thompson DH

更新日期：1998-02-03 00:00:00

abstract：:Accurate protein folding is essential for proper cellular and organismal function. In the cell, protein folding is carefully regulated; changes in folding homeostasis (proteostasis) can disrupt many cellular processes and have been implicated in various neurodegenerative diseases and other pathologies. For many protei...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Samelson AJ,Jensen MK,Soto RA,Cate JH,Marqusee S

更新日期：2016-11-22 00:00:00

abstract：:We have used a microfluidic mass sensor to measure the density of single living cells. By weighing each cell in two fluids of different densities, our technique measures the single-cell mass, volume, and density of approximately 500 cells per hour with a density precision of 0.001 g mL(-1). We observe that the intrins...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grover WH,Bryan AK,Diez-Silva M,Suresh S,Higgins JM,Manalis SR

更新日期：2011-07-05 00:00:00

abstract：:Whether Golgi enzymes remain localized within the Golgi or constitutively cycle through the endoplasmic reticulum (ER) is unclear, yet is important for understanding Golgi dependence on the ER. Here, we demonstrate that the previously reported inefficient ER trapping of Golgi enzymes in a rapamycin-based assay results...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sengupta P,Satpute-Krishnan P,Seo AY,Burnette DT,Patterson GH,Lippincott-Schwartz J

更新日期：2015-12-08 00:00:00

abstract：:Unfolded RNase A is known to contain an equilibrium mixture of two forms, a slow-folding form (U(1)) and a fast-folding form (U(2)). If U(1) is produced after unfolding by the slow cis-trans isomerization of proline residues about X-Pro imide bonds, then the formation of U(1) should be catalyzed by strong acids. There...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schmid FX,Baldwin RL

更新日期：1978-10-01 00:00:00

abstract：:In translation, elongation factor Tu (EF-Tu) molecules deliver aminoacyl-tRNAs to the mRNA-programmed ribosome. The GTPase activity of EF-Tu is triggered by ribosome-induced conformational changes of the factor that play a pivotal role in the selection of the cognate aminoacyl-tRNAs. We present a 6.7-A cryo-electron m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Villa E,Sengupta J,Trabuco LG,LeBarron J,Baxter WT,Shaikh TR,Grassucci RA,Nissen P,Ehrenberg M,Schulten K,Frank J

更新日期：2009-01-27 00:00:00

abstract：:BACE1 is the rate-limiting enzyme for amyloid-β peptides (Aβ) generation, a key event in the pathogenesis of Alzheimer's disease (AD). By an unknown mechanism, levels of BACE1 and a BACE1 mRNA-stabilizing antisense RNA (BACE1-AS) are elevated in the brains of AD patients, implicating that dysregulation of BACE1 expres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bahn G,Park JS,Yun UJ,Lee YJ,Choi Y,Park JS,Baek SH,Choi BY,Cho YS,Kim HK,Han J,Sul JH,Baik SH,Lim J,Wakabayashi N,Bae SH,Han JW,Arumugam TV,Mattson MP,Jo DG

更新日期：2019-06-18 00:00:00

abstract：:DNase I hypersensitive regions were mapped within the 17.3-kilobase qa (quinic acid) gene cluster of Neurospora crassa. The 5'-flanking regions of the five qa structural genes and the two qa regulatory genes each contain DNase I hypersensitive sites under noninducing conditions and generally exhibit increases in DNase...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baum JA,Giles NH

更新日期：1986-09-01 00:00:00

abstract：:The different isoforms of PDGF bind with different affinities to two different receptor types. Previously, these receptors have been demonstrated mainly on mesenchymal and glial cells. We show here that PDGF beta-type receptors are present also on rat brain neurons in vivo as well as in vitro. Immunohistochemical anal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smits A,Kato M,Westermark B,Nistér M,Heldin CH,Funa K

更新日期：1991-09-15 00:00:00

abstract：:A cylindrical detonating explosive behaves like a non-Newtonian viscous fluid emerging from a cylindrical pipe. The expression for non-Newtonian viscous flow has been applied to detonating explosives. The resultant fit to emerging flame fronts from detonation is excellent for a wide variety of detonating explosives bo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coon N,Ma SM,Redington PK,Brown B,Eyring H

更新日期：1980-10-01 00:00:00

abstract：:The type II cell of the alveolar epithelium of adult rats has been studied by electron microscopy to determine its ability to endocytose electron-dense tracers of differing molecular charge and size. The tracers studied were native (anionic) ferritin, cationic ferritin, 70-kilodalton dextran, and colloidal carbon. Alv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams MC

更新日期：1984-10-01 00:00:00

abstract：:The conformational change in amyloid beta (Abeta) peptide from its monomeric form to aggregates is crucial in the pathogenesis of Alzheimer's disease (AD). In the healthy brain, some unidentified chaperones appear to prevent the aggregation of Abeta. Here we reported that lipocalin-type prostaglandin D synthase (L-PGD...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kanekiyo T,Ban T,Aritake K,Huang ZL,Qu WM,Okazaki I,Mohri I,Murayama S,Ozono K,Taniike M,Goto Y,Urade Y

更新日期：2007-04-10 00:00:00