Structure and expression of the murine N-myc gene.

abstract：:Plasmodium parasites must complete development in the mosquito vector for transmission to occur. The mosquito innate immune response is remarkably efficient in limiting parasite numbers. Previous work has identified a LPS-induced TNFα transcription factor (LITAF)-like transcription factor, LITAF-like 3 (LL3), which si...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith RC,Barillas-Mury C,Jacobs-Lorena M

更新日期：2015-06-30 00:00:00

abstract：:Excitotoxicity, resulting from sustained activation of glutamate receptors of the N-methyl-d-aspartate (NMDA) subtype, is considered to play a causative role in the etiology of ischemic stroke and several neurodegenerative diseases. The NMDA receptor is therefore a target for the development of neuroprotective agents....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tai KK,Blondelle SE,Ostresh JM,Houghten RA,Montal M

更新日期：2001-03-13 00:00:00

abstract：:Influenza is a paradigm for understanding viral infections. As an opportunistic pathogen exploiting the cellular endocytic machinery for infection, influenza is also a valuable model system for exploring the cell's constitutive endocytic pathway. We have studied the transport, acidification, and fusion of single influ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lakadamyali M,Rust MJ,Babcock HP,Zhuang X

更新日期：2003-08-05 00:00:00

abstract：:We have found the two-dimensional electrophoretic technique of O'Farrell to be highly efficient in the detection of charge-change substitutions in a large number of proteins. We have applied this method to determine the level of heterozygosity of the most abundant proteins in Drosophila melanogaster adults from a natu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown AJ,Langley CH

更新日期：1979-05-01 00:00:00

abstract：:A cyclophilin (CyP) purified to homogeneity from the polycentric anaerobic rumen fungus Orpinomyces sp. strain PC-2 had a molecular mass of 20.5 kDa and a pI of 8.1. The protein catalyzed the isomerization of the prolyl peptide bond of N-succinyl-Ala-Ala-(cis,trans)-Pro-Phe p-nitroanilide with a kcat/Km value of 9.3 x...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen H,Li XL,Ljungdahl LG

更新日期：1995-03-28 00:00:00

abstract：:Most of the cell-surface molecules involved in T-cell immune responses are N-linked glycoproteins. We have investigated the effects of inhibitors of glycoprotein processing on specific T-cell functions, with the dual aims of examining the functional role of carbohydrate and of testing the usefulness of such compounds ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wall KA,Pierce JD,Elbein AD

更新日期：1988-08-01 00:00:00

abstract：:Despite extensive studies, the structural basis for the mechanochemical coupling in the rotary molecular motor F1-ATPase (F1) is still incomplete. We performed single-molecule FRET measurements to monitor conformational changes in the stator ring-α3β3, while simultaneously monitoring rotations of the central shaft-γ. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sugawa M,Okazaki K,Kobayashi M,Matsui T,Hummer G,Masaike T,Nishizaka T

更新日期：2016-05-24 00:00:00

abstract：:Multicomponent therapies, originating through deliberate mixing of drugs in a clinical setting, through happenstance, and through rational design, have a successful history in a number of areas of medicine, including cancer, infectious diseases, and CNS disorders. We have developed a high-throughput screening method f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Borisy AA,Elliott PJ,Hurst NW,Lee MS,Lehar J,Price ER,Serbedzija G,Zimmermann GR,Foley MA,Stockwell BR,Keith CT

更新日期：2003-06-24 00:00:00

abstract：:We predict by first principles a phase transition in alumina at approximately 3.7 Mbar and room temperature from the CaIrO(3)-type polymorph to another with the U(2)S(3)-type structure. Because alumina is used as window material in shock-wave experiments, this transformation should be important for the analysis of sho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Umemoto K,Wentzcovitch RM

更新日期：2008-05-06 00:00:00

abstract：:Intermolecular energy transfer between appropriately chosen pairs of dyes can be used to induce or enhance banding patterns in human metaphase chromosomes. Energy transfer, calibrated by fluorometric studies on soluble dye.DNA complexes, can also be detected by photometric measurements on cytological preparations of m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sahar E,Latt SA

更新日期：1978-11-01 00:00:00

abstract：:We consider the sequential estimation of p, the probability of success in an infinite sequence of Bernoulli trials, when the loss incurred in stopping after n trials and estimating p by some function delta(n) of the first n outcomes is taken to be [Formula: see text] The loss due to error of estimation is thus the sym...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cabilio P,Robbins H

更新日期：1975-01-01 00:00:00

abstract：:At the onset of apoptosis, the peroxidation of cardiolipin at the inner mitochondrial membrane by cytochrome c requires an open coordination site on the heme. We report a 1.45-Å resolution structure of yeast iso-1-cytochrome c with the Met80 heme ligand swung out of the heme crevice and replaced by a water molecule. T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McClelland LJ,Mou TC,Jeakins-Cooley ME,Sprang SR,Bowler BE

更新日期：2014-05-06 00:00:00

abstract：:Aortic endothelial cells from normal pigs and pigs with von Willebrand disease have been established in long-term cultures. Both cultures appeared similar in terms of general growth characteristics, morphologic features and ultrastructure. Immunofluorescent staining of these cultures with chicken (or rabbit) antiporci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Booyse FM,Quarfoot AJ,Bell S,Fass DN,Lewis JC,Mann KG,Bowie EJ

更新日期：1977-12-01 00:00:00

abstract：:Inspite of several stimulating developments in gene therapy, the formulation of a targeted gene delivery "vector" is still far from ideal. We have demonstrated the potential of reconstituted Sendai viral envelopes containing only the fusion glycoprotein (F-virosomes) in targeted delivery of reporter genes to liver cel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramani K,Hassan Q,Venkaiah B,Hasnain SE,Sarkar DP

更新日期：1998-09-29 00:00:00

abstract：:Multiple cues, across multiple sensory modalities, are involved in mate choice in a wide range of animal taxa. This multiplicity leads to the prediction that, in adaptive radiations, sexual isolation results from divergence in multiple dimensions. However, difficulties in directly measuring preferences and detecting m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hohenlohe PA,Arnold SJ

更新日期：2010-09-21 00:00:00

abstract：:The catalytic activity of low-dimensional electrocatalysts is highly dependent on their local atomic structures, particularly those less-coordinated sites found at edges and corners; therefore, a direct probe of the electrocatalytic current at specified local sites with true nanoscopic resolution has become critically...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun T,Wang D,Mirkin MV,Cheng H,Zheng JC,Richards RM,Lin F,Xin HL

更新日期：2019-06-11 00:00:00

abstract：:Circulating exosomal microRNA (miR) represents a new class of blood-based biomarkers for cancer liquid biopsy. The detection of miR at a very low concentration and with single-base discrimination without the need for sophisticated equipment, large volumes, or elaborate sample processing is a challenge. To address this...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Canady TD,Li N,Smith LD,Lu Y,Kohli M,Smith AM,Cunningham BT

更新日期：2019-09-24 00:00:00

abstract：:The 30S ribosomal subunit of E. coli was treated with the bifunctional reagent bis-(methyl)suberimidate. Crosslinked ribosomal proteins were identified as bands with increased molecular weight after electrophoresis in polyacrylamide gels containing sodium dodecyl sulphate. The pattern of crosslinked products was alter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bickle TA,Hershey JW,Traut RR

更新日期：1972-05-01 00:00:00

abstract：:N-Acetylvalyl-tRNA1Val (AcVal-tRNA1Val) was bound to the P site of uniformly 32P-labeled 70S ribosomes from Escherichia coli and crosslinked to 16S RNA in the 30S ribosomal subunit by irradiation with light of 300-400 nm. To identify the crosslinked nucleotide in 16S RNA. AcVal-tRNA1Val-16S [32P]RNA was digested compl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prince JB,Taylor BH,Thurlow DL,Ofengand J,Zimmermann RA

更新日期：1982-09-01 00:00:00

abstract：:Genes encoded by mitochondrial DNA (mtDNA) exist in large numbers per cell but can be selected very rapidly as a result of unequal partitioning of mtDNA between germ cells during embryogenesis. However, empirical studies of this "bottlenecking" effect are rare because of the apparent scarcity of heteroplasmic individu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Van Leeuwen T,Vanholme B,Van Pottelberge S,Van Nieuwenhuyse P,Nauen R,Tirry L,Denholm I

更新日期：2008-04-22 00:00:00

abstract：:The nodZ gene, which is present in various soil bacteria such as Bradyrhizobium japonicum, Azorhizobium caulinodans, and Rhizobium loti, is involved in the addition of a fucosyl residue to the reducing N-acetylglucosamine residue of lipochitin oligosaccharide (LCO) signal molecules. Using an Escherichia coli strain th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Quinto C,Wijfjes AH,Bloemberg GV,Blok-Tip L,López-Lara IM,Lugtenberg BJ,Thomas-Oates JE,Spaink HP

更新日期：1997-04-29 00:00:00

abstract：:We previously reported that KIF3A and KIF3B form a heterodimer that functions as a microtubule-based fast anterograde translocator of membranous organelles. We have also shown that this KIF3A/3B forms a complex with other associated polypeptides, named kinesin superfamily-associated protein 3 (KAP3). In the present st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamazaki H,Nakata T,Okada Y,Hirokawa N

更新日期：1996-08-06 00:00:00

abstract：:We propose approaching prediction from a framework grounded in the theoretical correct prediction rate of a variable set as a parameter of interest. This framework allows us to define a measure of predictivity that enables assessing variable sets for, preferably high, predictivity. We first define the prediction rate ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lo A,Chernoff H,Zheng T,Lo SH

更新日期：2016-12-13 00:00:00

abstract：:We have inserted a fourth protein ligand into the zinc coordination polyhedron of carbonic anhydrase II (CAII) that increases metal affinity 200-fold (Kd = 20 fM). The three-dimensional structures of threonine-199-->aspartate (T199D) and threonine-199-->glutamate (T199E) CAIIs, determined by x-ray crystallographic met...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ippolito JA,Baird TT Jr,McGee SA,Christianson DW,Fierke CA

更新日期：1995-05-23 00:00:00

abstract：:rev-regulated expression of HIV-1 envelope proteins from a simian virus 40 late replacement vector was found to be dependent on the presence of a 5' splice site in the env mRNA in spite of the fact that this mRNA remains unspliced. When the 5' splice site upstream of the env open reading frame was deleted or mutated, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu XB,Heimer J,Rekosh D,Hammarskjöld ML

更新日期：1990-10-01 00:00:00

abstract：:Resistance to thyroid hormone (RTH) is most often due to point mutations in the beta-isoform of the thyroid hormone (TH) receptor (TR-beta). The majority of mutations involve the ligand-binding domain, where they block TH binding and receptor function on both stimulatory and inhibitory TH response elements. In contras...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Machado DS,Sabet A,Santiago LA,Sidhaye AR,Chiamolera MI,Ortiga-Carvalho TM,Wondisford FE

更新日期：2009-06-09 00:00:00

abstract：:A line of nonobese diabetic (NOD) mice expressing the human diabetes-associated HLA-DQ8 transgene in the absence of mouse IAbeta failed to show spontaneous insulitis or diabetes, but rather developed dilated cardiomyopathy, leading to early death from heart failure. Pathology in these animals results from an organ- an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elliott JF,Liu J,Yuan ZN,Bautista-Lopez N,Wallbank SL,Suzuki K,Rayner D,Nation P,Robertson MA,Liu G,Kavanagh KM

更新日期：2003-11-11 00:00:00

abstract：:Language and analytic processing are currently thought to be represented in the left hemisphere, whereas spatial and holistic processing would involve primarily the right hemisphere in man. An experimental paradigm for engaging the nonlanguage hemisphere (generally the right) is described. This involves active touch e...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Desmedt JE

更新日期：1977-09-01 00:00:00

abstract：:Adenovirus (Ad) vectors have been extensively used to deliver recombinant genes to a great variety of cell types in vitro and in vivo. Ad-based vectors are available that replace the Ad early region 1 (E1) with recombinant foreign genes. The resultant E1-deleted vectors can then be propagated on 293 cells, a human emb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amalfitano A,Begy CR,Chamberlain JS

更新日期：1996-04-16 00:00:00

abstract：:The underpinnings of STAT3 hyperphosphorylation resulting in enhanced signaling and cancer progression are incompletely understood. Loss-of-function mutations of enzymes that dephosphorylate STAT3, such as receptor protein tyrosine phosphatases, which are encoded by the PTPR gene family, represent a plausible mechanis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lui VW,Peyser ND,Ng PK,Hritz J,Zeng Y,Lu Y,Li H,Wang L,Gilbert BR,General IJ,Bahar I,Ju Z,Wang Z,Pendleton KP,Xiao X,Du Y,Vries JK,Hammerman PS,Garraway LA,Mills GB,Johnson DE,Grandis JR

更新日期：2014-01-21 00:00:00