Enhancement of banding patterns in human metaphase chromosomes by energy transfer.

abstract：:Weakly oncogenic adenovirus 2 (Ad2)-transformed LSH hamster cells are sensitive to lysis by spontaneously cytolytic lymphoid cells and activated macrophages, whereas highly oncogenic simian virus 40 (SV40)-transformed LSH cells are relatively resistant to these nonspecific effector cells. Somatic cell hybrids formed b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cook JL,Hauser J,Patch CT,Lewis AM Jr,Levine AS

更新日期：1983-10-01 00:00:00

abstract：:A key energy-saving adaptation to chronic hypoxia that enables cardiomyocytes to withstand severe ischemic insults is hibernation, i.e., a reversible arrest of contractile function. Whereas hibernating cardiomyocytes represent the critical reserve of dysfunctional cells that can be potentially rescued, a lack of a sui...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： May D,Gilon D,Djonov V,Itin A,Lazarus A,Gordon O,Rosenberger C,Keshet E

更新日期：2008-01-08 00:00:00

abstract：:REPRESSOR OF SILENCING 1 (ROS1) is a DNA demethylation enzyme that was previously identified during a genetic screen for the silencing of both RD29A-LUC and 35S-NPTII transgenes on a T-DNA construct. Here we performed a genetic screen to identify additional mutants in which the 35S-NPTII transgene is silenced. We iden...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li X,Qian W,Zhao Y,Wang C,Shen J,Zhu JK,Gong Z

更新日期：2012-07-10 00:00:00

abstract：:We have shown that the silk fibroin gene from Bombyx mori is faithfully transcribed in an in vitro transcription system of the HeLa cell extract prepared by the method of Manley et al. [Manley, J. L., Fire, A., Cano, A., Sharp, P. A. & Gefter, M. L. (1980) Proc. Natl. Acad. Sci. USA 77, 3855-3859]. Using this system a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsujimoto Y,Hirose S,Tsuda M,Suzuki Y

更新日期：1981-08-01 00:00:00

abstract：:We provide evidence that citrate anions bridge between mineral platelets in bone and hypothesize that their presence acts to maintain separate platelets with disordered regions between them rather than gradual transformations into larger, more ordered blocks of mineral. To assess this hypothesis, we take as a model fo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davies E,Müller KH,Wong WC,Pickard CJ,Reid DG,Skepper JN,Duer MJ

更新日期：2014-04-08 00:00:00

abstract：:It has been hypothesized that penicillin acts as a structural analog of the acyl-D-alanyl-D-alanine terminus of nascent bacterial cell wall and that it consequently binds to and acylates the active site of the enzyme(s) that crosslinks the cell wall to form an inactive penicilloyl enzyme [Tipper, D.J. & Strominger, J....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yocum RR,Waxman DJ,Rasmussen JR,Strominger JL

更新日期：1979-06-01 00:00:00

abstract：:The drug resistance factor RP4, originally isolated in Pseudomonas, was transferred to Rhizobium meliloti. In that strain, RP4 promotes conjugational transfer of chromosomal markers to form haploid recombinants. This mating system has been used to construct a linkage map of R. meliloti. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meade HM,Signer ER

更新日期：1977-05-01 00:00:00

abstract：:The myocardin family proteins (myocardin, MRTF-A, and MRTF-B) are serum response factor (SRF) cofactors and potent transcription activators. Gene-ablation studies have indicated important developmental functions for myocardin family proteins primarily in regulation of cardiac and smooth muscle development. Using Xenop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meadows SM,Warkman AS,Salanga MC,Small EM,Krieg PA

更新日期：2008-02-05 00:00:00

abstract：:Sera from 30 patients with astrocytoma were tested for antibody reacting with cell surface antigens of cultured autologous astrocytoma cells. Ten percent of the patients had antibody detectable by mixed hemadsorption assays, approximately 50% by immune adherence and protein A assays, and 100% by anti-C3-mixed hemadsor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pfreundschuh M,Shiku H,Takahashi T,Ueda R,Ransohoff J,Oettgen HF,Old LJ

更新日期：1978-10-01 00:00:00

abstract：:Translation of injected mRNA in oocytes of Xenopus laevis has been used as a highly sensitive and quantitative assay for interferon mRNA. Injection into oocytes of polyadenylylated RNA extracted from poly(I).poly(C)-induced human diploid fibroblasts (FS-4) leads to the synthesis of biologically active human fibroblast...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sehgal PB,Dobberstein B,Tamm I

更新日期：1977-08-01 00:00:00

abstract：:Leukemia cell lines of the monocytic series (HL-60, U-937, and P388D1) produce a hepatocyte-stimulating factor (HSF) following induction of differentiation with phorbol diester. In 24-72 hr, these leukemia cells produce 2-30% the amount of HSF as human peripheral blood monocytes. Cells of the series at earlier stages ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Woloski BM,Fuller GM

更新日期：1985-03-01 00:00:00

abstract：:In flowering plants, extensive male-female interactions are required for successful fertilization in which various signaling cascades are involved. Prevacuolar compartments (PVC) and vacuoles are two types of subcellular compartments that terminate signal transduction by sequestrating signaling molecules in yeast and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hao L,Liu J,Zhong S,Gu H,Qu LJ

更新日期：2016-05-31 00:00:00

abstract：:We constructed a comprehensive cDNA library from HeLa cell mRNA in a vector that directs expression of the cDNA in Saccharomyces cerevisiae. We used this library to clone the human counterpart of the Sa. cerevisiae CCAAT-binding transcription factor, Hap2, by functional complementation of a hap2 mutation. The cDNA enc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Becker DM,Fikes JD,Guarente L

更新日期：1991-03-01 00:00:00

abstract：:A fraction of mouse RNA containing messenger RNA coding for mouse interferon was translated with high efficiency in a wheat germ system into a fully active product. This product fulfills the criteria for mouse interferon, namely: (1) it was active against vesicular stomatitis virus and herpes simplex virus in mouse ce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thang MN,Thang DC,De Maeyer E,Montagnier L

更新日期：1975-10-01 00:00:00

abstract：:Protective immunity against human schistosome infection develops slowly, for reasons that are not yet fully understood. For many decades, researchers have attempted to infer properties of the immune response from epidemiological studies, with mathematical models frequently being used to bridge the gap between immunolo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitchell KM,Mutapi F,Savill NJ,Woolhouse ME

更新日期：2012-08-14 00:00:00

abstract：:In vitro and in vivo triplet state electron paramagnetic resonance (epr) spectra of bacteriochlorophylls (Bchls) show important differences in (a) electron spin polarization (esp), and (b) zero field splitting (ZFS) parameters. The unusual esp and ZFS properties of the observed in vivo triplet state are best interpret...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thurnauer MC,Katz JJ,Norris JR

更新日期：1975-09-01 00:00:00

abstract：:A common challenge in pathogen discovery by deep sequencing approaches is to recognize viral or subviral pathogens in samples of diseased tissue that share no significant homology with a known pathogen. Here we report a homology-independent approach for discovering viroids, a distinct class of free circular RNA subvir...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu Q,Wang Y,Cao M,Pantaleo V,Burgyan J,Li WX,Ding SW

更新日期：2012-03-06 00:00:00

abstract：:The molecular mechanism by which the homodimeric enzyme Cu/Zn superoxide dismutase (SOD) causes neural damage in amytrophic lateral sclerosis is yet poorly understood. A striking, as well as an unusual, feature of SOD is that it maintains intrasubunit disulfide bonds in the reducing environment of the cytosol. Here, w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lindberg MJ,Normark J,Holmgren A,Oliveberg M

更新日期：2004-11-09 00:00:00

abstract：:C1q, a member of the immune complement cascade, is implicated in the selective pruning of synapses by microglial phagocytosis. C1q-mediated synapse elimination has been shown to occur during brain development, while increased activation and complement-dependent synapse loss is observed in neurodegenerative diseases. H...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Györffy BA,Kun J,Török G,Bulyáki É,Borhegyi Z,Gulyássy P,Kis V,Szocsics P,Micsonai A,Matkó J,Drahos L,Juhász G,Kékesi KA,Kardos J

更新日期：2018-06-12 00:00:00

abstract：:Cytogenetic analysis of acute myeloid leukemia (AML) cells has accelerated the identification of genes important for AML pathogenesis. To complement cytogenetic studies and to identify genes altered in AML genomes, we performed genome-wide copy number analysis with paired normal and tumor DNA obtained from 86 adult pa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walter MJ,Payton JE,Ries RE,Shannon WD,Deshmukh H,Zhao Y,Baty J,Heath S,Westervelt P,Watson MA,Tomasson MH,Nagarajan R,O'Gara BP,Bloomfield CD,Mrózek K,Selzer RR,Richmond TA,Kitzman J,Geoghegan J,Eis PS,Maupin R,

更新日期：2009-08-04 00:00:00

abstract：:Recent theoretical and experimental results point to the existence of small barriers to protein folding. These barriers can even be absent altogether, resulting in a continuous folding transition (i.e., downhill folding). With small barriers, the detailed properties of folding ensembles may become accessible to equili...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Muñoz V,Sanchez-Ruiz JM

更新日期：2004-12-21 00:00:00

abstract：:Twenty-four hours after rats receive choline chloride (20 mmol/kg, by stomach tube) the activity of tyrosine hydroxylase [tyrosine 3-monooxygenase; L-tyrosine, tetrahydropteridine:oxygen oxidoreductase (3-hydroxylating), EC 1.14.16.2] increases by 31% within adrenomedullary chromaffin cells. This treatment also causes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ulus IH,Hirsch MJ,Wurtman RJ

更新日期：1977-02-01 00:00:00

abstract：:We have analyzed the level of intraindividual sequence variability (heteroplasmy) of mtDNA in human brain by denaturing gradient gel electrophoresis and sequencing. Single base substitutions, as well as insertions or deletions of single bases, were numerous in the noncoding control region (D-loop), and 35-45% of the m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jazin EE,Cavelier L,Eriksson I,Oreland L,Gyllensten U

更新日期：1996-10-29 00:00:00

abstract：:Ubc13 is a ubiquitin-conjugating enzyme responsible for noncanonical ubiquitination of TNF receptor-associated factor (TRAF)-family adapter proteins involved in Toll-like receptor and TNF-family cytokine receptor signaling, which are regulators of innate immunity. Gene ablation was used to study the function of Ubc13 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fukushima T,Matsuzawa S,Kress CL,Bruey JM,Krajewska M,Lefebvre S,Zapata JM,Ronai Z,Reed JC

更新日期：2007-04-10 00:00:00

abstract：:A selenium-containing protein, selenoprotein A, is an essential component of the clostridial glycine reductase complex. This enzyme complex catalyzes the reductive deamination of glycine, which is coupled to the esterification of orthophosphate resulting in the formation of ATP. Sequence information was obtained by au...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sliwkowski MX,Stadtman TC

更新日期：1988-01-01 00:00:00

abstract：:Focally evoked calcium waves in astrocyte cultures have been thought to propagate by gap-junction-mediated intercellular passage of chemical signal(s). In contrast to this mechanism we observed isolated astrocytes, which had no physical contact with other astrocytes in the culture, participating in a calcium wave. Thi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hassinger TD,Guthrie PB,Atkinson PB,Bennett MV,Kater SB

更新日期：1996-11-12 00:00:00

abstract：:The study of development has relied primarily on the isolation of mutations in genes with specific functions in development and on the comparison of their expression patterns in normal and mutant phenotypes. Comparative evolutionary analyses can complement these approaches. Phylogenetic analyses of Sonic hedgehog (Shh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zardoya R,Abouheif E,Meyer A

更新日期：1996-11-12 00:00:00

abstract：:The two large cytoplasmic domains (C1 and C2) of adenylyl cyclases (AC), when expressed separately and mixed together, reconstitute enzyme activity that can be regulated by various modulators. Therefore, we have used the C1 or its C1a subdomain and C2 regions from type I AC (ACI) and type V AC (ACV) to identify the re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wittpoth C,Scholich K,Yigzaw Y,Stringfield TM,Patel TB

更新日期：1999-08-17 00:00:00

abstract：:The p21(WAF1) (p21) cyclin-dependent kinase inhibitor plays a major role in regulating cell cycle arrest. It was recently reported that the p53-independent elevation of p21 protein levels is essential in mediating the G(1) arrest resulting from signal transduction events initiated by the crosslinking of membrane IgM o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marches R,Hsueh R,Uhr JW

更新日期：1999-07-20 00:00:00

abstract：:Type IV pili (Tfp), which are key virulence factors in many bacterial pathogens, define a large group of multipurpose filamentous nanomachines widespread in Bacteria and Archaea. Tfp biogenesis is a complex multistep process, which relies on macromolecular assemblies composed of 15 conserved proteins in model gram-neg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goosens VJ,Busch A,Georgiadou M,Castagnini M,Forest KT,Waksman G,Pelicic V

更新日期：2017-06-20 00:00:00