Binding of a 3,3', 4,4'-tetrachlorobiphenyl (CB-77) metabolite to fetal transthyretin and effects on fetal thyroid hormone levels in mice.


:The present study was conducted in order to study the effect of the PCB congener 3,3', 4,4'-tetrachlorobiphenyl (CB-77) on fetal thyroxin homeostasis in the mouse, and to examine a possible underlying mechanism behind the effect. C57BL mice were treated with 14C-labelled or unlabelled CB-77 (1 or 10 mg/kg body wt.) on day 13 of gestation, and control animals were treated with corn oil. The experiment was terminated at 4 days after exposure. Maternal and fetal plasma and livers, and whole fetuses for homogenate preparation, were collected and analysed for total radioactivity, in vitro binding of 125I-thyroxin to plasma transthyretin (TTR; a thyroxin-transporting protein), and free and total thyroxin (FT4, TT4) levels. Maternal plasma, fetal plasma and homogenates were also analyzed for presence of CB-77 and metabolites. Results showed a dose-dependent uptake of radioactivity in plasma and liver, fetal plasma 14C-levels being about five-times higher in 10 mg/kg dosed animals as after 1 mg/kg. Fetal; plasma levels of total radioactivity were four- to nine-times above maternal levels and corresponded to only one compound, the metabolite 4-OH-3,3', 4',5-tetrachlorobiphenyl (4-OH-tCB). 4-OH-tCB was the major metabolite also in whole fetuses, with only small amounts of the parent compound (approximately 15% of the 4-OH-tCB) and traces (approximately 6%) of two other metabolites, 2-OH-3,3, 4,4'-tetrachlorobiphenyl and 5-OH-3,3', 4,4'-tetrachlorobiphenyl. Polyacrylamide gel electrophoresis confirmed that the 14C-radioactivity in fetal plasma was bound to TTR, and revealed that in vitro binding of 125I-T4 to fetal TTR was reduced to 50% of control values in treated animals (10 mg/kg body wt.). Fetal plasma FT4 and TT4 levels were significantly decreased (64 and 55% of control fetuses) after 10 mg/kg treatment. In conclusion, exposure of pregnant mice to CB-77 results in the accumulation of the metabolite 4-OH-tCB in fetal mouse plasma. The metabolite binds to TTR and is accompanied by a significant decrease in fetal plasma T4 levels. A causative correlation between TTR binding and effects on T4 levels is suggested.






Darnerud PO,Morse D,Klasson-Wehler E,Brouwer A




Has Abstract


1996-01-08 00:00:00














  • Effects of low concentrations of cadmium on immunoglobulin E production by human B lymphocytes in vitro.

    abstract::Exposure to cadmium (Cd) can cause a variety of biological effects including alterations of immune responses in animals and humans. Both immunosuppression and immunoenhancement have been reported. The present study was aimed at investigating the consequences of exposure to Cd on the human immunoglobulin (Ig) E synthes...


    pub_type: 杂志文章


    authors: Jelovcan S,Gutschi A,Kleinhappl B,Sedlmayr P,Barth S,Marth E

    更新日期:2003-06-03 00:00:00

  • Kinetics of amyl nitrite-induced hemoglobin oxidation in cord and adult blood.

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    pub_type: 杂志文章


    authors: Tarburton JP,Metcalf WK

    更新日期:1985-07-01 00:00:00

  • Evidence for aconitine-induced inhibition of delayed rectifier K(+) current in Jurkat T-lymphocytes.

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    pub_type: 杂志文章


    authors: Wu SN,Chen BS,Lo YC

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  • Insulin and dexamethasone inhibit TGF-beta-induced apoptosis of hepatoma cells upstream of the caspase activation cascade.

    abstract::Insulin and dexamethasone are potent inhibitors of apoptosis induced by transforming growth factor-beta1 (TGF-beta) in hepatoma cells. Using FTO-2B rat hepatoma cells, we determined whether the anti-apoptotic effects of these agents result from interference within or upstream of the TGF-beta-induced caspase cascade. A...


    pub_type: 杂志文章


    authors: Wanke I,Schwarz M,Buchmann A

    更新日期:2004-11-15 00:00:00

  • Effects of nitrated-polycyclic aromatic hydrocarbons and diesel exhaust particle extracts on cell signalling related to apoptosis: possible implications for their mutagenic and carcinogenic effects.

    abstract::Nitrated-polycyclic aromatic hydrocarbons (nitro-PAHs) and diesel exhaust particle extracts (DEPE) induced apoptosis in Hepa1c1c7 cells with the following potency: 1,3-dinitropyrene (1,3-DNP)>1-nitropyrene (1-NP) > DEPE > 1,8-dinitropyrene (1,8-DNP). The compounds induced cyp1a1, and activated various intracellular si...


    pub_type: 杂志文章


    authors: Landvik NE,Gorria M,Arlt VM,Asare N,Solhaug A,Lagadic-Gossmann D,Holme JA

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  • Effect of Cd administration on the pituitary-adrenal axis.

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    pub_type: 杂志文章


    authors: Hidalgo J,Armario A

    更新日期:1987-07-01 00:00:00

  • Hepatic clotrimazole concentrations and hepatic drug metabolizing enzyme activities in adult male Sprague-Dawley rats.

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    pub_type: 杂志文章


    authors: Pappas JB,Franklin MR

    更新日期:1993-06-04 00:00:00

  • Evaluation of the potential immunotoxicity of chlorinated drinking water in mice.

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    pub_type: 杂志文章


    authors: French AS,Copeland CB,Andrews DL,Wiliams WC,Riddle MM,Luebke RW

    更新日期:1998-01-16 00:00:00

  • Hematological changes produced in mice by ochratoxin A and citrinin.

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    pub_type: 杂志文章


    authors: Gupta M,Sasmal D,Bandyopadhyay S,Bagchi G,Chatterjee T,Dey S

    更新日期:1983-01-01 00:00:00

  • Increased carbon tetrachloride hepatotoxicity after low-level ethanol consumption.

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    pub_type: 杂志文章


    authors: Strubelt O,Obermeier F,Siegers CP,Vöpel M

    更新日期:1978-07-01 00:00:00

  • Lipoic acid increases glutathione production and enhances the effect of mercury in human cell lines.

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    pub_type: 杂志文章


    authors: Hultberg B,Andersson A,Isaksson A

    更新日期:2002-06-14 00:00:00

  • Kinetic analysis of the toxicological effect of tacrine (Cognex) on human retinal acetylcholinesterase activity.

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    pub_type: 杂志文章


    authors: Alhomida AS,Al-Rajhi AA,Kamal MA,Al-Jafari AA

    更新日期:2000-05-19 00:00:00

  • Safety evaluation of an açai-fortified fruit and berry functional juice beverage (MonaVie Active(®)).

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    pub_type: 杂志文章


    authors: Obata T

    更新日期:2006-01-20 00:00:00

  • Factors affecting induction of lung aryl hydrocarbon hydroxylase in rats exposed to cigarette smoke.

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    pub_type: 杂志文章


    authors: Down WH,Chasseaud LF,Sacharin RM

    更新日期:1981-01-01 00:00:00

  • Deltamethrin-induced oxidative damage and biochemical alterations in rat and its attenuation by Vitamin E.

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    pub_type: 杂志文章


    authors: Klein RG

    更新日期:1982-01-01 00:00:00

  • Suspended particulate matter in indoor air: adjuvants and allergen carriers.

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    authors: Ormstad H

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  • Behavioral effects in adult mice exposed to perfluorooctane sulfonate (PFOS).

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    authors: Levine BS,Preache MM,Pergament E

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  • Distortion product otoacoustic emissions as non-invasive biomarkers and predictors of soman-induced central neurotoxicity: a preliminary study.

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    authors: Yamano T,Shimizu M,Noda T

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    authors: Gupta S,Pandey SD,Misra V,Viswanathan PN

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    pub_type: 杂志文章


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  • Long-term cadmium exposure accelerates age-related mitochondrial changes in renal epithelial cells.

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    authors: Takaki A,Jimi S,Segawa M,Hisano S,Takebayashi S,Iwasaki H

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    pub_type: 杂志文章


    authors: Foster PM,Lloyd SC,Blackburn DM

    更新日期:1987-01-01 00:00:00

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    pub_type: 杂志文章


    authors: Nogueira CW,Meotti FC,Curte E,Pilissão C,Zeni G,Rocha JB

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    pub_type: 杂志文章


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    pub_type: 杂志文章,评审


    authors: Singh DP,Bagam P,Sahoo MK,Batra S

    更新日期:2017-05-15 00:00:00