Abstract:
OBJECTIVE:To evaluate binding characteristics of a specific zona pellucida (ZP) protein 3 (ZP3) antiserum to human oocytes in order to determine its usefulness as a clinical marker for human ZP integrity and function and its correlation with IVF outcome. DESIGN:Prospectively designed, blinded, internally controlled study. SETTING:Tertiary care academic center. PATIENTS:Patients undergoing IVF therapy who had either total failed fertilization or partial fertilization were studied. INTERVENTIONS:Metaphase II oocytes showing absence of pronuclear formation were salt stored 48 hours after insemination and bisected into matching hemizonae using micromanipulation. One hemizona was incubated with AS ZP3-6 (an antiserum generated against a synthetic ZP3 peptide derived from an amino acid sequence that is highly conserved in the structure of ZP3), whereas the matching hemizona was incubated with AS ZP3-7, an antiserum detecting exclusively mouse ZP3 (internal, negative control). Antibody binding was visualized using the peroxidase-antiperoxidase method and diaminobenzidine as color reagent. RESULTS:A total of 104 unfertilized oocytes were evaluated. Analysis of variance showed a significant interaction between gamete factor groups (sperm and oocyte) and antiserum factor. Patients with oocyte factor had significantly lower mean staining scores for the AS ZP3-6-treated hemizonae than patients with sperm factor. CONCLUSIONS:These results demonstrate that anomalies of human ZP3 can be identified with AS ZP3-6 and that these ZP abnormalities correlate with fertilization failure during IVF treatment. Thus, this newly developed biomarker may be of clinical significance in the identification of oocyte defects that are associated with fertilization disorders and may help in the decision-making process in the IVF-assisted fertilization setting.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Oehninger S,Hinsch E,Pfisterer S,Veeck LL,Kolm P,Schill WB,Hodgen GD,Hinsch KDdoi
10.1016/s0015-0282(16)58041-8subject
Has Abstractpub_date
1996-01-01 00:00:00pages
139-45issue
1eissn
0015-0282issn
1556-5653pii
S0015-0282(16)58041-8journal_volume
65pub_type
杂志文章abstract:OBJECTIVE:Analysis of sperm heads using three different computer morphometrical tools and experimental conditions to find a more reliable and secure strategy among them. DESIGN:Controlled experiments on sperm morphology analysis from volunteers. SETTING:Laboratory of microscopy and imaging processing. PATIENT(S):Ten...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(03)00978-6
更新日期:2003-09-01 00:00:00
abstract::Polycystic ovary syndrome presents with many different symptoms and signs, for which different therapies are indicated. However, for the majority of women, the metabolic disorder requires diligence in management, regardless of the presenting complaint. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.03.011
更新日期:2006-07-01 00:00:00
abstract:OBJECTIVE:To report a case of a methotrexate (MTX)-resistant heterotopic interstitial pregnancy in which dactinomycin was necessary as a second-line chemotherapy. DESIGN:Case report. SETTING:Medical college-affiliated hospital. PATIENT(S):A 39-year-old woman with vaginal bleeding after the transfer of two thawed 8-c...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.10.003
更新日期:2009-03-01 00:00:00
abstract:OBJECTIVE:To determine if increases in core body temperature preceding most hot flashes are caused by increased metabolic rate, peripheral vasoconstriction, or central noradrenergic activation. DESIGN:Laboratory physiological study. SETTING:University medical center. PATIENT(S):Fourteen healthy, postmenopausal women...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/s0015-0282(98)00137-x
更新日期:1998-08-01 00:00:00
abstract::The cumulative conception rate of 31 infertile women undergoing a second tuboplasty using microsurgery was compared with that 71 women undergoing a single microsurgical tuboplasty. The follow-up period ranged from ranged from 1 to 61/2 years. Five (16%) of the patients in the repeat tuboplasty group conceived; there w...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)45979-0
更新日期:1982-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the effects of laparoscopic bipolar coagulation of uterine vessels in treating symptomatic fibroids. DESIGN:Prospective clinical study. SETTING:University-affiliated tertiary referral center. PATIENT(S):Eighty-seven women with symptomatic fibroids warranting surgical treatment and wanting to re...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(00)01724-6
更新日期:2001-02-01 00:00:00
abstract:OBJECTIVE:To analyze the potential role of extraovarian factors such as endometrium in the reproductive outcome of obese patients. DESIGN:Retrospective study. SETTING:University-affiliated infertility clinic, between January 2001 and July 2005. PATIENT(S):Women undergoing a total of 2656 first ovum donation (OD) cyc...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.11.162
更新日期:2007-08-01 00:00:00
abstract:OBJECTIVE:To evaluate by thorough pathologic analysis the histologic features of the endometrioma wall excised at laparoscopy. DESIGN:Prospective series of consecutive patients. SETTING:Tertiary care, university hospital. PATIENT(S):Fifty-nine patients with ovarian endometriomas. A total of 70 cysts were examined. ...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/j.fertnstert.2006.06.055
更新日期:2007-02-01 00:00:00
abstract:OBJECTIVE:To investigate the serum pattern of free leptin, bound leptin, and soluble leptin receptor throughout the physiological menstrual cycle. DESIGN:Prospective observational study. SETTING:Tertiary care center for gynecological endocrinology and reproductive medicine and a university research laboratory. PATIE...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2003.06.020
更新日期:2004-02-01 00:00:00
abstract::Deoxyribonucleic acid (DNA) flow cytometry of testicular tissue has been demonstrated to be a quantitative means of assessing spermatogenesis. This study evaluates testes aspirates and DNA flow cytometry in the evaluation of the infertile male. Testicular tissue obtained from 12 men who underwent bilateral orchiectomy...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)59358-3
更新日期:1987-08-01 00:00:00
abstract:OBJECTIVE:To investigate the functional and sexual outcome of sigmoid vaginoplasty in patients with Mayer-Rokitansky-Kuster-Hauser (MRKH) syndrome. DESIGN:Prospective study. SETTING:University hospital. PATIENT(S):Fifty-nine consecutive patients with MRKH syndrome. INTERVENTION(S):Forty-eight patients underwent sig...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2011.12.015
更新日期:2012-03-01 00:00:00
abstract:OBJECTIVE:To determine the sensitivity and clinical significance of fluorescein isothiocyanate wheat germ agglutinin (FITC-WGA) staining of human spermatozoa. DESIGN:Percentage FITC-WGA staining was assessed in the different morphological zones of human spermatozoa and compared with fertilization rates of metaphase II...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)57499-8
更新日期:1995-04-01 00:00:00
abstract:OBJECTIVE:To assess the efficiency of the addition of pentoxifylline to spermatozoa for in vitro fertilization (IVF) in cases with previous fertilization failure. DESIGN:After retrieval, oocytes were divided at random and inseminated with spermatozoa prepared with or without pentoxifylline. SETTING:In vitro fertiliza...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(16)55641-6
更新日期:1993-01-01 00:00:00
abstract:OBJECTIVE:To investigate whether a poor rate of implantation after in vitro fertilization (IVF) was due to poor embryonic/endometrial synchrony during the original IVF cycle, we have cryopreserved reinseminated-fertilized embryos for later more synchronous replacement after thawing. The chance of implantation of fresh ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)54725-6
更新日期:1991-12-01 00:00:00
abstract::Changes in the composition of media used for human in vitro fertilization and embryo culture are reviewed, and their impact on improved pregnancy rates is discussed. It is concluded that changes to the formulation of culture media for assisted reproductive technologies over the past decade have played a significant ro...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2003.10.003
更新日期:2004-01-01 00:00:00
abstract::Direct effects of a gonadotropin-releasing hormone agonist (GnRHa), danazol, or estrogen/progestogen (E/P) on experimental endometriosis were evaluated in castrated female rats. Endometrial explants decreased in size following castration, but there was no further change in the treatment groups. Histologic examination ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)59727-1
更新日期:1988-02-01 00:00:00
abstract::This study estimated birth order-specific miscarriage rates and characterized the influence of maternal age using the complete birth history of 90,303 ever-married women from the 1998-2000 Indian National Family Health Survey. Rates of miscarriage in India were low in association with early childbearing, whereas birth...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2005.02.023
更新日期:2005-08-01 00:00:00
abstract:OBJECTIVE:To critically examine ovarian reserve testing before assisted reproduction. DESIGN:A PUBMED computer search to identify relevant literature. SETTING:Multiple sites. PATIENT(S):Patients undergoing assisted reproduction. INTERVENTION(S):Testing for ovarian reserve. MAIN OUTCOME MEASURE(S):Assisted reproduc...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2007.10.080
更新日期:2008-12-01 00:00:00
abstract:OBJECTIVE:Both pronuclear morphology and early zygote cleavage have been used in combination with day 3 criteria to predict implantation and pregnancy. However, in routine practice, it is impractical to use both these criteria to select embryos on day 3. The objective of the present study was to find which of the two c...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2005.07.1319
更新日期:2006-02-01 00:00:00
abstract::Uterus transplantation research has been conducted toward its introduction in the human as a treatment of absolute uterine-factor infertility, which is considered to be the last frontier to conquer for infertility research. In this review we describe the patient populations that may benefit from uterus transplantation...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2012.04.001
更新日期:2012-06-01 00:00:00
abstract:OBJECTIVE:To evaluate sperm DNA fragmentation in normozoospermic male partners of couples undergoing infertility evaluation. DESIGN:Retrospective cohort study. SETTING:Clinical andrology laboratory. PATIENT(S):A total of 1,974 consecutive normozoospermic men selected from a larger cohort of 4,345 consecutive, nonazo...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2014.02.006
更新日期:2014-06-01 00:00:00
abstract:OBJECTIVE:To determine whether complete failure of fertilization of oocytes in couples with unexplained infertility persists during subsequent in vitro fertilization (IVF) cycles. DESIGN:A retrospective study of 120 cycles of IVF in 44 couples with unexplained infertility and complete failure of fertilization during t...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)55677-5
更新日期:1993-02-01 00:00:00
abstract::A case of coma due to embolization after HSG, using oil-soluble contrast medium is presented. The patient was in a comatose state 11 days and then spontaneously regained full consciousness. The safety of oil-contrast medium for HSG is questionable. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)53537-7
更新日期:1990-05-01 00:00:00
abstract::Transferrin and somatomedin C receptors (TFr and SMCr) were studied in human ovaries by immunostaining using monoclonal antisera. The oocyte of evoluting follicles was intensely positive for both types of receptors, but this positivity decreases in large follicles. An evident positivity for both TFr and SMCr was shown...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)59533-8
更新日期:1987-11-01 00:00:00
abstract::Danazol and gestrinone are effective drugs in the treatment of endometriosis. Their mechanism of action remains uncertain, but may be related to their androgenic activity. The authors examined the effect of danazol on human endometrial cells cultured in vitro, its two major metabolites, ethisterone and 2 hydroxymethyl...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)59706-4
更新日期:1988-02-01 00:00:00
abstract:OBJECTIVE:To determine the efficacy of combined l-carnitine and l-acetyl-carnitine therapy in infertile males with oligo-astheno-teratozoospermia. DESIGN:Placebo-controlled double-blind randomized trial. SETTING:University tertiary referral center. PATIENT(S):Sixty infertile patients (aged 20-40 years) with the foll...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2003.10.034
更新日期:2004-06-01 00:00:00
abstract:OBJECTIVE:To compare clinical outcomes of frozen embryo transfers using cryopreserved pronuclear stage oocytes that had undergone either intracytoplasmic sperm injection (ICSI) or conventional IVF. DESIGN:Observational. SETTING:A tertiary referral reproductive medicine unit. PATIENT(S):Couples undergoing either ICSI...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/s0015-0282(99)00444-6
更新日期:1999-12-01 00:00:00
abstract::The cytochrome P-450 1A1 (CYPA1A) gene plays an important role in the metabolization of estrogen and is therefore a candidate marker for fibroids. In a case-control study, we were unable to demonstrate any association between MSP I CYP1A1 polymorphism and the risk of leiomyoma in Brazilian women. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.05.034
更新日期:2010-12-01 00:00:00
abstract:OBJECTIVE:To review, evaluate, and synthesize current published reports assessing the value of abdominal myomectomy in infertile women and those desiring to preserve fertility potential. DATA IDENTIFICATION:Major studies dealing with myomectomy were identified through Medline Searches. STUDY SELECTION:Those papers re...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/s0015-0282(16)55128-0
更新日期:1992-07-01 00:00:00
abstract:OBJECTIVE:To define the hypersensitive status of PRL secretion in normoprolactinemic infertile women and determine the incidence of occult hyperprolactinemia among them. DESIGN:The potential for PRL secretion was examined in 463 women. SETTING:Outpatient clinic in a university hospital. PATIENTS:Three hundred sixty-...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)56154-8
更新日期:1993-09-01 00:00:00