Aspiration flow cytometry of the testes in the evaluation of spermatogenesis in the infertile male.

Abstract:

:Deoxyribonucleic acid (DNA) flow cytometry of testicular tissue has been demonstrated to be a quantitative means of assessing spermatogenesis. This study evaluates testes aspirates and DNA flow cytometry in the evaluation of the infertile male. Testicular tissue obtained from 12 men who underwent bilateral orchiectomy for prostate cancer (group 1) were examined by both flow cytometry and standard histologic technique to assess the correlation between these two modalities. Thirteen men evaluated for infertility (group 2) and requiring histologic evaluation of spermatogenesis underwent both open biopsy and fine needle aspiration of their testes. Histology was independently examined and grouped according to standard nomenclature. Flow cytometric analysis revealed characteristic patterns in the relative numbers of haploid (1C), diploid (2C), and tetraploid (4C) cells. These patterns correlated reproducibly with the histologic diagnoses. DNA flow cytometry of testicular aspirates provides a rapid and reliable quantitative means of assessing spermatogenesis.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Kaufman DG,Nagler HM

doi

10.1016/s0015-0282(16)59358-3

subject

Has Abstract

pub_date

1987-08-01 00:00:00

pages

287-91

issue

2

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(16)59358-3

journal_volume

48

pub_type

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