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Sequential antibody affinity chromatography of human leukocyte interferon.

Abstract：

:Antibodies to crude leukocyte interferon (CIF) were bound to Sepharose 4B, and CIF was chromatographed. A relationship between recovered interferon and percentage binding of the antibodies to Sepharose 4B was observed. Highest recovery was obtained when only 50%-85% of th- antibodies were bound. When CIF was subjected to normal affinity chromatography, specific activity of 1-5 X 10(6) interferon units (IFU) (69/19B-units)/mg protein was obtained. When this purified preparation was further chromatographed on a column mainly directed against normal cellular proteins and then concentrated by normal affinity chromatography, a tenfold increase in 'direct' specific activity was obtained. It is suggested that the 'indirect' specific activity is about 2 X 10(8) IFU/mg protein.

journal_name

Scand J Immunol

journal_title

Scandinavian journal of immunology

authors

Berg K

doi

10.1111/j.1365-3083.1977.tb00323.x

subject

Has Abstract

pub_date

1977-01-01 00:00:00

pages

77-86

issue

1-2

eissn

0300-9475

issn

1365-3083

journal_volume

6

pub_type

杂志文章

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