Immunoadsorbent assay application for studies of protein mixtures and usage of paper carriers.

abstract：:To further investigate the interaction between human mononuclear leucocytes [peripheral blood mononuclear cells (PBMC)] and enterocytes, the effect of a confluent layer of differentiated CACO-2 cells on cytokine kinetics during challenge with bacteria in a compartmentalized coculture model was investigated. Nonpathoge...

journal_title：Scandinavian journal of immunology

authors： Parlesak A,Haller D,Brinz S,Baeuerlein A,Bode C

更新日期：2004-11-01 00:00:00

abstract：:The mechanisms by which glucocorticoids suppress immune responses have not yet been clearly defined. In steroid-sensitive pathological conditions, an increase in gamma delta T cells can occur in certain untreated systemic autoimmune disorders and seems to be a peristent feature in most cases of systemic lupus erythema...

journal_title：Scandinavian journal of immunology

authors： Spinozzi F,Agea E,Bistoni O,Travetti A,Migliorati G,Moraca R,Nicoletti I,Riccardi C,Paoletti FP,Vaccaro R

更新日期：1995-05-01 00:00:00

abstract：:This study describes an immunomagnetic bead-based methodology for optimal purification of mouse CD4+ T cells. It reproducibly yields highly pure CD4+ T cells from mouse lymph nodes (95-99%) and spleen (93-96%) with no residual antigen-presenting cell (APC) function in the purified population. The recovery of the start...

journal_title：Scandinavian journal of immunology

authors： Kamala T

更新日期：2008-03-01 00:00:00

abstract：:CD8+ T cells are thought to play an important role in protective immunity against tuberculosis. We report the identification of three peptides derived from Rv1818c, Rv3812 and Rv3018c proteins of Mycobacterium tuberculosis that bound to HLA-A*0201 molecules and their ability to induce in vitro T-cell response in perip...

journal_title：Scandinavian journal of immunology

authors： Chaitra MG,Shaila MS,Chandra NR,Nayak R

更新日期：2008-04-01 00:00:00

abstract：:Human mast cell carboxypeptidase (hMC-CP) is a unique product of mast cells. Unlike tryptase and chymase, its potential function and expression in diseased conditions remain largely unknown. To develop an assay for hMC-CP, the recombinant fusion protein of hMC-CP and purified native skin hMC-CP was prepared, and two n...

journal_title：Scandinavian journal of immunology

authors： Zhou Y,Chen Z,He S

更新日期：2006-11-01 00:00:00

abstract：:The relationship between the so-called natural interferon-alpha (IFN-alpha) producing cell (IPC), stimulated to produce IFN-alpha by herpes simplex virus type 1 (HSV), and of dendritic cells (DC) in peripheral blood leucocytes was investigated. The simultaneous expression of cell surface antigens and intracellular IFN...

journal_title：Scandinavian journal of immunology

authors： Svensson H,Johannisson A,Nikkilä T,Alm GV,Cederblad B

更新日期：1996-08-01 00:00:00

abstract：:We have evaluated phagocytosis, C3-binding and cytotoxicity of human leukocytes simultaneously, using IgM-sensitized C3-fragment-bearing sheep erythrocytes (EAC3b, EAC3bi) as targets. In this method, 51Cr-labelled EAC3b or EAC3bi were added to human peripheral granulocytes anchored by fibronectin onto microtitre plate...

journal_title：Scandinavian journal of immunology

authors： Kojima A,Hazeki K,Seya T

更新日期：1991-06-01 00:00:00

abstract：:Phosphorothioate oligodeoxynucleotides (sODNs) can induce T-cell-independent polyclonal activation of human B cells by a mechanism that depends on both sequence and back-bone structure. Because matrix-bound as well as soluble sODNs are mitogenic, this stimulation may result from the engagement of surface receptor(s). ...

journal_title：Scandinavian journal of immunology

authors： Liang H,Reich CF,Pisetsky DS,Lipsky PE

更新日期：2001-12-01 00:00:00

abstract：:Cells of the mononuclear phagocytic system (MPS) were described as playing a decisive role in amyloidogenesis. A relationship between the amyloid enhancing factor (AEF) and MPS cells was suggested and recently AEF activity was attributed to a serine esterase (SE) of leucocytic origin. In the present study, no correlat...

journal_title：Scandinavian journal of immunology

authors： Niewold TA,Gruys E,Kisilevsky R,Shirahama TS

更新日期：1991-07-01 00:00:00

abstract：:Cutaneous leishmaniasis (CL) is a self-healing skin disease which rarely for unknown reason(s) the lesion develops to a non-healing form. It seems that the initial contact of Leishmania parasites with the host innate immune system is an important step in the outcome of the disease. Recent studies suggested that toll-l...

journal_title：Scandinavian journal of immunology

authors： Tolouei S,Hejazi SH,Ghaedi K,Khamesipour A,Hasheminia SJ

更新日期：2013-11-01 00:00:00

abstract：:In a longitudinal analysis of immunological changes in normal human pregnancy, twenty-two pregnant women were studied, with blood samples taken before pregnancy, during its course, at delivery, and 3-5 months after delivery. The blood samples were frozen successively, using a cryobiological freezing system, and were s...

journal_title：Scandinavian journal of immunology

authors： Birkeland SA,Kristoffersen K

更新日期：1980-01-01 00:00:00

abstract：:The relationship of velocity of blood flow to density and microanatomical distribution of inflammatory cells in the dermis was studied in 20 human tuberculin tests. Most positive reactions showed maximal blood flow velocities (measured as red blood cell (RBC) flux) at the centre of the reaction, but the two most inten...

journal_title：Scandinavian journal of immunology

authors： Swanson Beck J,Gibbs JH,Potts RC,Jawad ES,Kardjito T,Spence VA,Grange JM

更新日期：1989-01-01 00:00:00

abstract：:RUTI is a vaccine consisting of Mycobacterium tuberculosis bacilli grown in stress conditions that is fragmented, detoxified and liposomed. RUTI was designed to shorten the treatment of latent tuberculosis infection (LTBI) with isoniazid from 9 months to just 1 month, by additional treatment with two inoculations of R...

journal_title：Scandinavian journal of immunology

authors： Vilaplana C,Ruiz-Manzano J,Gil O,Cuchillo F,Montané E,Singh M,Spallek R,Ausina V,Cardona PJ

更新日期：2008-06-01 00:00:00

abstract：:Pneumocystis carinii is an opportunistic pathogen that causes pneumonia in immunocompromised hosts. In the normal host, P. carinii is susceptible to an array of first line host defense mechanisms that are operative in the lung. Alveolar macrophages play a central role in the clearance of inhaled organisms. The macroph...

journal_title：Scandinavian journal of immunology

authors： Stehle SE,Rogers RA,Harmsen AG,Ezekowitz RA

更新日期：2000-08-01 00:00:00

abstract：:After intravenous injection of 10(5) purified, lymph node (LN)-derived dm2 (H-2d/Ld-) CD4+ T cells into young C.B-17 scid/scid (severe combined immunodeficiency, SCID) mice (H-2d/Ld+), the transplanted Ld-T cells show a selective pattern of engraftment: they repopulate the spleen, the lamina propria of the small intes...

journal_title：Scandinavian journal of immunology

authors： Rudolphi A,Enssle KH,Claesson MH,Reimann J

更新日期：1993-07-01 00:00:00

abstract：:In murine schistosomiasis mansoni the worm egg-induced granulomatous inflammation is bi-phasic: an initial Th1 type is subsequently switched to a Th2 type response. Analysis of the cellular, molecular base of the Th1-associated response (5-6 weeks post infection) revealed mRNA messages for interleukin (IL)-12 p40, IL-...

journal_title：Scandinavian journal of immunology

authors： Todt JC,Whitfield JR,Ivard SR,Boros DL

更新日期：2000-10-01 00:00:00

abstract：:The activation of complement and the release of TNF-alpha, IL-6 and IL-8 are important pathogenic factors behind organ dysfunction in sepsis. The aim of this study was to determine whether infusion of anti-TNF antibodies alters complement activation and plasma concentrations of pro-inflammatory cytokines at high doses...

journal_title：Scandinavian journal of immunology

authors： Bengtsson A,Redl H,Schlag G,Högåsen K,Götze O,Mollnes TE

更新日期：1998-11-01 00:00:00

abstract：:The demonstration in the 1960s that T cells collaborated with B cells in enabling antibody responses to simple protein antigens opened up the challenge of how to investigate the interactions of two rare antigen-specific lymphocytes within a vast population. One idea was that T cells made soluble factors that could act...

journal_title：Scandinavian journal of immunology

authors： Waldmann H

更新日期：2005-07-01 00:00:00

abstract：:The authors have previously reported on the ability of A60, an immunodominant antigenic complex of Mycobacterium bovis BCG, to prevent cancer development in mice challenged with EMT 6 tumour cells. Such effect proved to rely on neoplastic cell lysis by cytolytic T lymphocytes and activated macrophages. The involvement...

journal_title：Scandinavian journal of immunology

authors： Maes H,Cocito C

更新日期：1996-10-01 00:00:00

abstract：:Previous investigations have indicated that C3H X CBA hybrid lymphocytes can neutralize lymphocytes which are reactive against the C3H-determined Mls antigen when injected into the H-2-compatible strain CBA. In this study we have inoculated C3H X CBA hybrids with CBA spleen cells and 2 weeks later lethally irradiated ...

journal_title：Scandinavian journal of immunology

authors： Lilliehöök B,Blomgren H

更新日期：1978-01-01 00:00:00

abstract：:There is convincing evidence that certain combinations of alleles within the human leucocyte antigen (HLA) complex, particularly within HLA-DQ, are associated with either resistance or susceptibility to insulin-dependent diabetes mellitus (IDDM). A previous study conducted on a large, well-defined group of patients de...

journal_title：Scandinavian journal of immunology

authors： Baisch JM,Capra JD

更新日期：1992-08-01 00:00:00

abstract：:Various surface molecules are expressed by activated T cells. Among them, the CD30 antigen has been proposed as a reproducible marker that identifies a subset of differentiated and/or activated T lymphocytes that produce T helper (Th)-2-type cytokines, i.e. interleukin (IL)-4 and IL-5. However, because CD30 has mainly...

journal_title：Scandinavian journal of immunology

authors： Spinozzi F,Agea E,Piattoni S,Falini B,Grignani F,Bertotto A

更新日期：1997-04-01 00:00:00

abstract：:The major rheumatoid factor cross-reactive idiotype, which was defined by prototypic monoclonal IgM rheumatoid factors from Waldenstrom's macroglobulinaemia patient Wa, is present on 60% of all monoclonal IgM RF paraproteins. One-third of patients with juvenile rheumatoid arthritis (JRA), who are seronegative for clas...

journal_title：Scandinavian journal of immunology

authors： Ilowite NT,Wedgwood JF,Moore TL,Ramakrishnan T,Bonagura VR

更新日期：1991-10-01 00:00:00

abstract：:Ulcerative colitis (UC) is an idiopathic, chronic inflammatory disease, which is characterized with overactive immune response. It is well established that the imbalance between Tregs and Th17 cells plays a pivotal role in pathogenesis of UC. In this study, we investigated the impact of functional changes in Treg subs...

journal_title：Scandinavian journal of immunology

authors： Long Y,Zhao X,Xia C,Li X,Fan C,Liu C,Wang C

更新日期：2020-06-01 00:00:00

abstract：:RadLV-Rs infection induces a murine immunodeficiency syndrome associated with a dramatic enlargement of spleen and lymph nodes. Surprisingly, the lymphoproliferation excludes thymus and Peyer's patches (PP). To understand the cellular interactions underlying lymphoproliferation further, the authors investigated the fa...

journal_title：Scandinavian journal of immunology

authors： Colombi S,Moutschen M,de Leval L,Demoitié MA,Trebak M,Martin MT,Boniver J

更新日期：1997-02-01 00:00:00

abstract：:Human blood lymphocytes were tested for their proliferative response to purified protein derivative (PPD) in vitro. Purified T cells showed a lower response than the orginial purified lymphocyte suspension. Purified B cells were unresponsive to PPD. Mixtures of preparations of T and B cells were stimulated to a greate...

journal_title：Scandinavian journal of immunology

authors： Blomgren H

更新日期：1975-09-01 00:00:00

abstract：:Chronic granulomatous disease (CGD) is a primary immunodeficiency characterized by susceptibility to bacterial and fungal infections resulting from the inadequacy of phagocytic leucocytes to produce reactive oxygen radicals. CGD is a genetically heterogeneous disease with an X-linked recessive (XR-CGD) form caused by ...

journal_title：Scandinavian journal of immunology

authors： Kutukculer N,Aykut A,Karaca NE,Durmaz A,Aksu G,Genel F,Pariltay E,Cogulu Ö,Azarsız E

更新日期：2019-02-01 00:00:00

abstract：:We describe a new quantitative fluorimetric assay for phagocytosis of bacteria. A suspension of fluorescein-labelled bacteria (Micrococcus lysodeikticus) is mixed and incubated with phagocytes. After fixation with paraformaldehyde, the excess non-phagocytosed and adsorbed bacteria are lysed with a solution of lysozyme...

journal_title：Scandinavian journal of immunology

authors： Vray B,Hoebeke J,Saint-Guillain M,Leloup R,Strosberg AD

更新日期：1980-01-01 00:00:00

abstract：:The mixed lymphocyte culture (MLC) response of lymphocytes from CBA mice against C3H cells was studied after injection of spleen cells from C3H mice or C3H hybrids. Intravenous infusion of C3H cells resulted in a strongly suppressed specific MLC response, but this was not the case when cells from H-2-incompatible hybr...

journal_title：Scandinavian journal of immunology

authors： Lilliehöök B,Jacobsson H,Blomgren H

更新日期：1975-01-01 00:00:00

abstract：:Novel immunogenic antigens are continually required for the improvement of diagnostic techniques for Mycobacterium tuberculosis infection. Some proteins with serodiagnostic value are not expressed under normal culture conditions, but may be induced under specific conditions such as gradual oxygen depletion and low pH,...

journal_title：Scandinavian journal of immunology

authors： Shin SJ,Kim SY,Shin AR,Kim HJ,Cho SN,Park JK

更新日期：2009-11-01 00:00:00