Abstract:
:Multiple cellular and molecular interactions are required for the differentiation and development of central neurons. For example, neural activity may modulate trophic function. In the developing cerebellum, establishment of functional excitatory synaptic connections coincides with the expression of NGF and its receptors. We have previously shown that excitatory signals and NGF act in concert to regulate the survival and morphological differentiation of cerebellar Purkinje cells in culture. To begin investigating the molecular mechanisms by which trophic interactions and neural activity modulate cerebellar development, we have now studied the role of excitatory signals on the expression of both NGF and the p75 glycoprotein (the low-affinity component of the NGF receptor) by cerebellar cells in culture. We used p75 as a model of potential responsiveness, since it is well characterized and conveniently monitored. Expression of the NGF and p75 mRNA's was studied in either mixed, neuron-enriched, or pure glial cultures. Expression of the NGF gene was localized to proliferating glial cells, while expression of p75 was restricted to developing Purkinje cells. To evaluate whether presynaptic activation may potentially modulate trophic factor receptor expression, the expression of the p75 gene was studied in cultures exposed to excitatory signals. Depolarization of cultures with high potassium, veratridine, or exposure to the excitatory neurotransmitter aspartate, resulted in a two- to threefold increase in the expression of both the p75 protein and messenger RNA. These increases did not require the presence of glia, indicating a direct effect of the excitatory signals on the neuronal population. Moreover, message and receptor increased per neuron. Our study suggests that local glia provide trophic support for Purkinje cell development, and that impulse activity modulates Purkinje cell responsiveness by regulating expression of trophic receptor subunits.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Cohen-Cory S,Elliott RC,Dreyfus CF,Black IBdoi
10.1006/exnr.1993.1018subject
Has Abstractpub_date
1993-02-01 00:00:00pages
165-75issue
2eissn
0014-4886issn
1090-2430pii
S0014-4886(83)71018-6journal_volume
119pub_type
杂志文章abstract::Bombesin/gastrin-releasing peptide (BB/GRP) receptors were characterized in fetal cortex transplants in the rat. Using in vitro autoradiography techniques, the density of (125I-Tyr4)BB grains was low 2 weeks after transplantation but increased 4 weeks after cortex transplantation into the adult rat fourth ventricle. D...
journal_title:Experimental neurology
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journal_title:Experimental neurology
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2017.10.023
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journal_title:Experimental neurology
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journal_title:Experimental neurology
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2014.09.004
更新日期:2015-01-01 00:00:00
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journal_title:Experimental neurology
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doi:10.1016/0014-4886(84)90065-7
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.2001.7734
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pub_type: 杂志文章
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1997.6601
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pub_type: 杂志文章
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1993.1050
更新日期:1993-04-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(84)90147-x
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journal_title:Experimental neurology
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journal_title:Experimental neurology
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journal_title:Experimental neurology
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2005.07.009
更新日期:2005-11-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2011.05.018
更新日期:2011-09-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(83)90270-4
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journal_title:Experimental neurology
pub_type: 杂志文章
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journal_title:Experimental neurology
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journal_title:Experimental neurology
pub_type: 杂志文章
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abstract::This study describes the short-term effects of intracaudate microinjections of carbachol in temporarily isolated and restrained Java monkeys. The monkeys were found to display a series of motor disturbances including blepharoptosis, facial twitches, tongue protrusions, ear flattening, torticollis, and compulsive alter...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(85)90133-5
更新日期:1985-01-01 00:00:00