L-histidine-mediated enhancement of hydrogen peroxide-induced cytotoxicity: relationships between DNA single/double strand breakage and cell killing.


:Results presented in this study demonstrate an association between the L-Histidine-mediated enhancement of H2O2-induced cytotoxicity and the formation of DNA double strand breakage (DSB), whereas no relationship exists between the increased cytotoxic response and DNA single strand breakage (SSB). Indeed, the higher lethality and the production of DNA DSB occurred in oxidatively-injured cells regardless of whether the exposure to L-Histidine was performed before or during challenge with the oxidant. In fact, the increased level of DNA SSB detected in cells simultaneously exposed to the oxidant and the amino acid was not observed in cells pre-treated with L-Histidine and then challenged with hydrogen peroxide. Further experiments have demonstrated an association between the kinetics of DNA DSB formation and the enhancement of the cytotoxic response. In conclusion, intracellular L-Histidine seems to mediate the formation of DNA DSB and the increased growth-inhibitory response elicited by the oxidant. In addition, these results suggest that the enhancement of DNA SSB is produced by the extracellular/plasma membrane fraction of the amino acid and not causally related to the L-Histidine-mediated increase of the growth-inhibitory response to H2O2-treated cells.


Pharmacol Res


Pharmacological research


Cantoni O,Guidarelli A,Sestili P,Giacomoni PU,Cattabeni F




Has Abstract


1994-02-01 00:00:00














  • The distribution of catecholamines between plasma and platelets in cyclosporin A-induced hypertensive rats.

    abstract::One of the most serious side effects of the immunosuppressive agent, cyclosporin A (CsA), is drug-related hypertension. As it is generally accepted, the sympathetic nervous system may play an important role in the development or maintenance of this abnormal pathophysiological situation. This study is aimed at assessin...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Reis F,Tavares P,Teixeira F

    更新日期:2000-02-01 00:00:00

  • Dysfunctional oleoylethanolamide signaling in a mouse model of Prader-Willi syndrome.

    abstract::Prader-Willi syndrome (PWS), the leading genetic cause of obesity, is characterized by a striking hyperphagic behavior that can lead to obesity, type-2 diabetes, cardiovascular disease and death. The molecular mechanism underlying impaired satiety in PWS is unknown. Oleoylethanolamide (OEA) is a lipid mediator involve...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Igarashi M,Narayanaswami V,Kimonis V,Galassetti PM,Oveisi F,Jung KM,Piomelli D

    更新日期:2017-03-01 00:00:00

  • A2BP1 gene polymorphisms association with olanzapine-induced weight gain.

    abstract::The ataxin-2 binding protein 1 (A2BP1) gene is reported to be one of the susceptibility genes in schizophrenia, autism, and obesity. The aim of this study was to explore the association of A2BP1 gene polymorphisms with antipsychotic induced weight gain (AIWG) in Chinese Han population. Three hundred and twenty-eight p...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Dong L,Yan H,Huang X,Hu X,Yang Y,Ma C,Du B,Lu T,Jin C,Wang L,Yu H,Dong Z,Li W,Ruan Y,Zhang H,Zhang H,Mi W,Ma W,Li K,Lv L,Zhang D,Yue W

    更新日期:2015-09-01 00:00:00

  • The effects of dexamethasone on lipid peroxidation and nitric oxide levels on the healing of tracheal anastomoses: an experimental study in rats.

    abstract::Corticosteroids are shown to have deleterious effects on wound healing for various tissues. Arginine metabolism and nitric oxide (NO) synthesis play an important role in many aspects of inflammation and wound healing. The study was designed to evaluate the relationship of dexamethasone impaired healing of tracheal ana...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Talas DU,Nayci A,Polat G,Atis S,Comelekoglu U,Bagdatoglu OT,Bagdatoglu C

    更新日期:2002-09-01 00:00:00

  • T cell toxicity of HIV latency reversing agents.

    abstract::Combination antiretroviral therapy reduces morbidity and mortality in HIV infected patients. However, the cure of HIV infection is hindered by the persistence of the latent HIV reservoir. Latency reversing agents (LRAs) are developed to target the HIV latently infected cells for HIV reactivation. In addition to revers...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Zhao M,De Crignis E,Rokx C,Verbon A,van Gelder T,Mahmoudi T,Katsikis PD,Mueller YM

    更新日期:2019-01-01 00:00:00

  • Drug glucuronidation and hepatic lipid microsomal membrane profile in cholestatic rats followed paracetamol intoxication.

    abstract::The uridin-diphosphoglucuronyl-transferase (UDP-GT) is a membrane-bound enzyme responsible for glucuronidation of endogenous and exogenous compounds. This work established the UDP-GT activity and its lipid membrane microenvironment in two experimental models: acute paracetamol intoxication, and cholestasis followed by...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Bengochea L,Ghanem C,Perazzo JC,Ghisolfi C,Marabotto L,Acevedo C,Mino J,Lemberg A,Rubio M

    更新日期:1999-10-01 00:00:00

  • Lipid-regulating properties of butyric acid and 4-phenylbutyric acid: Molecular mechanisms and therapeutic applications.

    abstract::In the past two decades, significant advances have been made in the etiology of lipid disorders. Concomitantly, the discovery of liporegulatory functions of certain short-chain fatty acids has generated interest in their clinical applications. In particular, butyric acid (BA) and its derivative, 4-phenylbutyric acid (...

    journal_title:Pharmacological research

    pub_type: 杂志文章,评审


    authors: He B,Moreau R

    更新日期:2019-06-01 00:00:00

  • Influence of PLA2-PG system on purine release and cAMP content in dissociated primary glial cultures from rat striatum.

    abstract::Purine release and prostaglandin (PG) outflow were simultaneously evaluated from untreated glial primary cultures of rat striatum, at rest and under field electrical stimulation. Purine release was also assayed from sister cultured cells in which a suitable pharmacological treatment with 1 x 10(-6) M dexamethasone or ...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Caciagli F,Ciccarelli R,Di Iorio P,Tacconelli L,Ballerini P

    更新日期:1989-05-01 00:00:00

  • Biochemical changes on the cardioprotective effect of nicorandil and amlodipine during experimental myocardial infarction in rats.

    abstract::The synergistic protective effect of nicorandil (KATP channel opener) and amlodipine (calcium channel blocker) on mitochondrial respiration and mitochondrial lipid contents were examined on isoproterenol-induced myocardial infarction in rats. The rats given isoproterenol (150 mg kg(-1) daily, i.p.) for 2 days showed s...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Sathish V,Ebenezar KK,Devaki T

    更新日期:2003-12-01 00:00:00

  • Ketamine protects acetylcholinesterase against inhibition by propoxur and phoxim.

    abstract::In the present study the effect of ketamine on the contractions caused by propoxur and phoxim on the isolated guinea pig ileum was investigated. Ketamine was found able to inhibit in a concentration-dependent manner the contractile responses of the ileum to propoxur and phoxim, while it did not significantly modify th...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Koutsoviti-Papadopoulou M,Kounenis G,Elezoglou V

    更新日期:1994-08-01 00:00:00

  • Effect of benzyl isothiocyanate on spontaneous and induced force of rat uterine contraction.

    abstract::The present study examines the effect of benzyl isothiocyanate (BITC) on uterine contraction in vitro. BITC (10-320 microM) caused irreversible, concentration-dependent inhibition of the spontaneous, prostaglandin F(2alpha) (PGF(2alpha)) and oxytocin-induced force of gravid and non-gravid rat uterine contractions in c...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Adebiyi A,Adaikan PG,Prasad RN

    更新日期:2004-05-01 00:00:00

  • More than just an enzyme: Dipeptidyl peptidase-4 (DPP-4) and its association with diabetic kidney remodelling.

    abstract:PURPOSE OF THE REVIEW:This review article discusses recent advances in the mechanism of dipeptidyl peptidase-4 (DPP-4) actions in renal diseases, especially diabetic kidney fibrosis, and summarizes anti-fibrotic functions of various DPP-4 inhibitors in diabetic nephropathy (DN). RECENT FINDINGS:DN is a common complica...

    journal_title:Pharmacological research

    pub_type: 杂志文章,评审


    authors: Gupta S,Sen U

    更新日期:2019-09-01 00:00:00

  • Open questions in bioequivalence.

    abstract::Bioequivalence studies are mainly required to demonstrate the interchangeability of multisource pharmaceutical products, usually called generics. A pivotal investigation projected and conducted in compliance with specific guidelines allows a replicative company to register a generic drug via an ANDA (abbreviated new d...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Marzo A

    更新日期:1995-10-01 00:00:00

  • Protective action of a new benzofuran derivative on lipid peroxidation and sulphydryl groups oxidation.

    abstract::The antioxidant properties of a novel water-soluble antioxidant of the benzofuran family (5-hydroxy-4,6,7-trimethyl-2,3-dihydrobenzofuran-2-acetic acid, BFA) were studied. In rat liver mitochondria BFA increases the lag-time and decreases the extent of lipid peroxidation induced by ascorbate/Fe2+; an IC50 value of abo...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Bindoli A,Rigobello MP,Musacchio E,Scuri R,Rizzoli V,Galzigna L

    更新日期:1991-12-01 00:00:00

  • Allosteric MEK1/2 inhibitors including cobimetanib and trametinib in the treatment of cutaneous melanomas.

    abstract::The Ras-Raf-MEK-ERK (Map kinase) cellular pathway is a highly conserved eukaryotic signaling module that transduces extracellular signals from growth factors and cytokines into intracellular regulatory events that are involved in cell growth and proliferation or the contrary pathway of cell differentiation. Dysregulat...

    journal_title:Pharmacological research

    pub_type: 杂志文章,评审


    authors: Roskoski R Jr

    更新日期:2017-03-01 00:00:00

  • Evaluation of 137Cs activity in plant drugs and in some phytoderivatives from Chernobyl accident up to present (1986-1994).

    abstract::The accident to the nuclear power plant of Chernobyl (in April 1986) caused a radioactive contamination in large areas of the majority of European countries. During the first transient time the fall-out phenomenon was the most important method of contamination, particularly from 131I whose relative isotopic abundance ...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Curini M,Rosati O,Borio R,Saetta DM,Cicioni R,Forini N,Rongoni A,Dipilato AC

    更新日期:1995-07-01 00:00:00

  • Influence of a 3-day regimen of azithromycin on the disposition kinetics of cyclosporine A in stable renal transplant patients.

    abstract::Some macrolide antibiotics have been shown to produce significant drug-drug interactions through the inhibition of cytochrome P450 (CYP) enzymes. In renal transplant patients these interactions pose potentially serious problems for the safe administration of cyclosporine A (CSA), a substrate of CYP3A4. The effects of ...

    journal_title:Pharmacological research

    pub_type: 临床试验,杂志文章


    authors: Bachmann K,Jauregui L,Chandra R,Thakker K

    更新日期:2003-06-01 00:00:00

  • Treatment duration affects cytoprotective efficacy of positive allosteric modulation of α7 nAChRs after focal ischemia in rats.

    abstract::To minimize irreversible brain injury after acute ischemic stroke (AIS), the time to treatment (i.e., treatment delay) should be minimized. However, thus far, all cytoprotective clinical trials have failed. Analysis of literature identified short treatment durations (≤72 h) as a common motif among completed cytoprotec...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Gaidhani N,Uteshev VV

    更新日期:2018-10-01 00:00:00

  • HIF-1α-dependent autophagy protects HeLa cells from fenretinide (4-HPR)-induced apoptosis in hypoxia.

    abstract::Novel therapeutic strategies are needed to address and to solve the emerging problem of hypoxia-induced resistance to anticancer drugs. N-(4-Hydroxyphenyl)retinamide (4-HPR) exhibits potent anticancer and chemopreventive activities, but its inefficiency under hypoxia, through undetermined mechanisms, may contribute to...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Liu XW,Su Y,Zhu H,Cao J,Ding WJ,Zhao YC,He QJ,Yang B

    更新日期:2010-11-01 00:00:00

  • The pathogenesis of renal injury in obstructive jaundice: A review of underlying mechanisms, inducible agents and therapeutic strategies.

    abstract::Kidney injury is one of the main complications of obstructive jaundice (OJ) and its pathogenesis has not been clarified. As an independent risk factor for OJ associated with significant morbidity and mortality, it can be mainly divided into two types of morphological injury and functional injury. We called these dysfu...

    journal_title:Pharmacological research

    pub_type: 杂志文章,评审


    authors: Liu J,Qu J,Chen H,Ge P,Jiang Y,Xu C,Chen H,Shang D,Zhang G

    更新日期:2021-01-01 00:00:00

  • Influence of mianserin on some central effects of ethanol.

    abstract::The interaction of mianserin with ethanol in central nervous system (CNS) was investigated. Mianserin was administered at a single dose of 5 or 20 mgkg(-1) i.p. or as daily injections in a dose of 2.5 mgkg(-1) given for 14 days. The influence of mianserin on acute ethanol toxicity (LD(50)), on ED(50) of ethanol in rot...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Pietrzak B,Kubik-Bogucka E

    更新日期:2002-07-01 00:00:00

  • Therapeutic effects of I(f) blockade: evidence and perspective.

    abstract::Heart rate slowing has been accepted for decades as a primary therapeutic approach to prevention (and even to treatment) of angina pectoris. Pure heart rate slowing has not been achieved with previously available rate-slowing pharmacological agents (beta adrenergic blockers, certain calcium channel blockers), all of w...

    journal_title:Pharmacological research

    pub_type: 杂志文章,评审


    authors: Borer JS

    更新日期:2006-05-01 00:00:00

  • Safety issues and harmful pharmacological interactions of nutritional supplements in Duchenne muscular dystrophy: considerations for Standard of Care and emerging virus outbreaks.

    abstract::At the moment, little treatment options are available for Duchenne muscular dystrophy (DMD). The absence of the dystrophin protein leads to a complex cascade of pathogenic events in myofibres, including chronic inflammation and oxidative stress as well as altered metabolism. The attention towards dietary supplements i...

    journal_title:Pharmacological research

    pub_type: 杂志文章,评审


    authors: Boccanegra B,Verhaart IEC,Cappellari O,Vroom E,De Luca A

    更新日期:2020-08-01 00:00:00

  • Prefrontal-accumbens opioid plasticity: Implications for relapse and dependence.

    abstract::In addiction, an individual's ability to inhibit drug seeking and drug taking is thought to reflect a pathological strengthening of drug-seeking behaviors or impairments in the capacity to control maladaptive behavior. These processes are not mutually exclusive and reflect drug-induced modifications within prefrontal ...

    journal_title:Pharmacological research

    pub_type: 杂志文章,评审


    authors: Hearing M

    更新日期:2019-01-01 00:00:00

  • Cellular and molecular mechanisms underlying alcohol-induced aggressiveness of breast cancer.

    abstract::Breast cancer is a leading cause of morbidity and mortality in women. Both Epidemiological and experimental studies indicate a positive correlation between alcohol consumption and the risk of breast cancer. While alcohol exposure may promote the carcinogenesis or onset of breast cancer, it may as well enhance the prog...

    journal_title:Pharmacological research

    pub_type: 杂志文章,评审


    authors: Wang Y,Xu M,Ke ZJ,Luo J

    更新日期:2017-01-01 00:00:00

  • Urinary pharmacokinetics of betalains following consumption of red beet juice in healthy humans.

    abstract::The aim of the present pilot study was to characterise the renal elimination of betalains after consumption of red beet juice (RBJ). Six healthy, non-smoking female volunteers were given a single oral dose of either 500 mL of a commercial RBJ containing 362.7 mg of betalains and 500 mL of tap water, respectively, in a...

    journal_title:Pharmacological research

    pub_type: 临床试验,杂志文章


    authors: Frank T,Stintzing FC,Carle R,Bitsch I,Quaas D,Strass G,Bitsch R,Netzel M

    更新日期:2005-10-01 00:00:00

  • alpha-Synuclein: a therapeutic target for Parkinson's disease?

    abstract::Parkinson's disease is a progressive age-related neurodegenerative disease with invariant loss of substantia nigra dopamine neurons and striatal projections. This disorder is well known for the associated motoric symptoms including resting tremor and the inability to initiate movement. However, it is now apparent that...

    journal_title:Pharmacological research

    pub_type: 杂志文章,评审


    authors: Maguire-Zeiss KA

    更新日期:2008-11-01 00:00:00

  • Molecular modeling study on resistance of WT/D473H SMO to antagonists LDE-225 and LEQ-506.

    abstract::The smoothened (SMO) receptor, an essential signal transducer in the Hedgehog pathway, was targeted with antagonists to suppress the tumor. It is interesting that SMO D473H mutation confers resistance on inhibitor LDE-225 rather than LEQ-506. In this paper, the binding modes of them against the wild type and mutant SM...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Tu J,Li JJ,Song LT,Zhai HL,Wang J,Zhang XY

    更新日期:2018-03-01 00:00:00

  • NESS06SM reduces body weight with an improved profile relative to SR141716A.

    abstract::We have recently synthesized a new series of 4,5-dihydrobenzo-oxa-cycloheptapyrazole derivatives with the aim to discover novel CB1 antagonist agents characterized by anti-obesity activity comparable to that of SR141716A but with reduced adverse effects such as anxiety and depression. Within the novel class, the CB1 a...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Mastinu A,Pira M,Pinna GA,Pisu C,Casu MA,Reali R,Marcello S,Murineddu G,Lazzari P

    更新日期:2013-08-01 00:00:00

  • A TCM formula comprising Sophorae Flos and Lonicerae Japonicae Flos alters compositions of immune cells and molecules of the STAT3 pathway in melanoma microenvironment.

    abstract::A traditional Chinese medicine (TCM) formula (SL) comprising Sophorae Flos and Lonicerae Japonicae Flos was used for treating melanoma in ancient China. We have previously shown that an ethanolic extract of SL (SLE) possesses anti-melanoma effects and suppresses STAT3 signaling in vitro and in vivo. STAT3 has been lin...

    journal_title:Pharmacological research

    pub_type: 杂志文章


    authors: Liu YX,Bai JX,Li T,Fu XQ,Guo H,Zhu PL,Chan YC,Chou JY,Yin CL,Li JK,Wang YP,Chen YJ,Yu ZL

    更新日期:2019-04-01 00:00:00