Abstract：
:Nickel, cobalt and chromium are metals very often implicated in allergic contact dermatitis. In vivo, keratinocytes, which are the first target cells, can be directly activated to participate in the local reaction, especially through the expression of the membrane antigen ICAM-1, a ligand of the leucocyte antigen LFA-1, and the production of cytokines. Our aim was to assess the effects of sensitizing metal haptens (nickel, cobalt and chromium) compared with the toxic metal cadmium on the induction of ICAM-1 and the production of TNF alpha by epidermal cells. For this purpose, normal human keratinocytes obtained during plastic skin surgery were cultured in low-calcium defined medium (MCDB153) and the metals were used in non-toxic concentrations. Using FACS analysis, ICAM-1 expression was found to be induced only by nickel. This stimulation appeared as early as 24 h after stimulation. All the metals induced a low expression of TNF alpha detectable by immunocytochemistry correlating with the induction of the nuclear stress protein Hsp72 which is closely linked genetically with the TNF alpha locus. However, only Ni2+, Co2+ and Cr2+ induced a significant release of TNF alpha detectable by ELISA after 48 h stimulation. This secretion was lower than that observed with known stimulants such as lipopolysaccharide. These results indicate that the metals studied are able to induce an aggressive cellular effect, and that nickel, by its ICAM-1 induction, may play a major role in the keratinocyte activation state during allergic contact dermatitis.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Guéniche A,Viac J,Lizard G,Charveron M,Schmitt Ddoi
10.1007/BF00371573subject
Has Abstractpub_date
1994-01-01 00:00:00pages
466-70issue
8eissn
0340-3696issn
1432-069Xjournal_volume
286pub_type
杂志文章abstract：:To evaluate the proliferative activity of benign, borderline and malignant cutaneous melanocytic neoplasms, 30 cases of malignant melanoma (MM) and 41 cases of naevi were studied by immunostaining using a monoclonal antibody against proliferating cell nuclear antigen (PCNA). PCNA is a nuclear antigen expressed in the ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00372033
更新日期：1992-01-01 00:00:00
abstract：:Based on reports suggesting aberrant cell-mediated immunity and altered infiltration of immunocompetent cells into the skin in psoriasis, we studied the stimulation of T cells by autologous non-T mononuclear leukocytes (autologous mixed lymphocyte reaction, AMLR) and by epidermal cells isolated from lesional and clini...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00417528
更新日期：1986-01-01 00:00:00
abstract：:Intercellular adhesion molecule-1 (ICAM-1) is regularly expressed or inducible on all major cutaneous cell populations including Langerhans cells, keratinocytes, endothelial cells and dermal fibroblasts. ICAM-1 is induced in the skin under inflammatory conditions and plays an important role in the activation of T cell...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050339
更新日期：1998-09-01 00:00:00
abstract：:The expression of IL-8 in psoriasis has been clearly shown with the use of immunocytochemical, RT-PCR and in situ hybridization methods. The presence of its ligand, the IL-8 receptor, has been demonstrated by the RT-PCR technique. We report here a study of the expression of both IL-8 type A and B receptors by immunohi...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050218
更新日期：1997-07-01 00:00:00
abstract：:Biopsy specimens from mixed connective tissue disease (MCTD) and discoid lupus erythematodes (DLE) skin lesions were stained with monoclonal antibodies to differentiation and activation antigens. In addition, the blast cells were studied by combining autoradiography with immunoperoxidase staining. In both disease cond...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00412679
更新日期：1988-01-01 00:00:00
abstract：:Recently, we discovered that New Zealand rabbits immunized with human type V collagen plus Freund's adjuvant present fibrosis and vasculitis of organs usually affected by systemic sclerosis. In this way, we studied the fibrillogenesis process to identify possible factors involved in altered remodeling observed in this...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-006-0645-5
更新日期：2006-07-01 00:00:00
abstract：:The stratum corneum, which is the outermost layer of the skin, functions as an important barrier to maintain biological homeostasis. The multilamellar structures formed by intercellular lipids present in the stratum corneum are considered to play an important role in barrier function. Most intercellular lipids are unb...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030000160
更新日期：2000-09-01 00:00:00
abstract：:The case of a 44-year-old man with a primary cutaneous large B-cell non-Hodgkin's lymphoma of the scalp is reported. His mother died of gastric lymphoma and his sib brother is in a 20-year remission of T-cell lymphoma. The patient presented with a 16-year history of occipital and parietal alopecia and a recently worse...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-009-0967-1
更新日期：2009-10-01 00:00:00
abstract：:Using the sebum-absorbent tape technique, we have disclosed five different patterns of follicular sebum excretion rate (SER). These are the infantile, pubertal, acne, adult and aging patterns, distinguished by different densities of active sebaceous follicles, by distinct levels of follicular SER and by the relative s...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00585931
更新日期：1987-01-01 00:00:00
abstract：:Vitiliginous patients undergoing photochemotherapy with TMP were less susceptible to a sun burn. To investigate this point, the MED for UV-B was measured before, 2 h, and 24 h after oral intake of TMP in 10 healthy volunteers. There was an increase of MED when UV exposure had taken place 2 h after TMP intake (P < 0.01...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00403890
更新日期：1980-01-01 00:00:00
abstract：:In a group of 40 patients suffering from wide-spread psoriasis oral administration of a retinoid (Ro 10-9359) was followed by PUVA therapy. The clearance rate was increased by 30% as compared to PUVA alone. Except for cheilitis no side effects were seen. Histological analysis in 20 patients before, during and after th...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00412661
更新日期：1979-05-04 00:00:00
abstract：:Using immunoblot and immunofluorescence analysis, we demonstrated the presence and localization of an immunoreactive form of erythrocyte ankyrin in human epidermal keratinocytes. Immunoblot analysis revealed that both human epidermis and cultured epidermal keratinocytes contained ankyrin-like proteins of molecular mas...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF02505038
更新日期：1996-01-01 00:00:00
abstract：:A low-molecular-weight proteinase inhibitor was isolated from human epidermal extracts by means of ultrafiltration, gel filtration and ion-exchange chromatography. On polyacrylamide gel electrophoresis (PAGE), the isolated fraction exhibited a single band of activity at pH 9.4, and inhibitory activity against papain a...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00412923
更新日期：1986-01-01 00:00:00
abstract：:The hair cycling pattern differs between humans and mice: for the former it is mosaic, while the latter show a wave pattern. We focused on the presence or absence of synchronization of the hair cycle based on the distribution of Merkel cells. The three-dimensional distribution and number of Merkel cells in the skin at...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050474
更新日期：2000-04-01 00:00:00
abstract：:Vitiligo is an acquired clinical conundrum, characterized by white areas on the skin due to loss of functional melanocytes. Various studies demonstrated that vitiligo is associated with fewer risk of skin cancer but no exact molecular explanation for this interesting observation has been described. This article review...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/s00403-011-1165-5
更新日期：2011-11-01 00:00:00
abstract：:The majority of studies on psoriasis have focused on explaining the genetic background and its associations with the immune system's response. The aim of this study was to identify the low-molecular weight compounds contributing to the metabolomic profile of psoriasis and to provide computational models that help with...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-017-1760-1
更新日期：2017-09-01 00:00:00
abstract：:A monoclonal antibody (Ted-R-1) to the keratohyalin granules (KHGs) of the newborn rat epidermis was developed by immunizing mice with a pH 4.7 hematoxylin-stainable fraction of the extract in a 50 mM Tris-HCl buffer, pH 7.3, containing 10 micrograms/ml phenylmethylsulfonyl fluoride (PMSF) from the newborn rat whole e...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00427657
更新日期：1989-01-01 00:00:00
abstract：:Eighteen patients with atopic dermatitis were randomly chosen for a clinical trial to compare the action of the H2 receptor antagonist cimetidine in combination with the H1 receptor antagonist chorpheniramine against the chlorpheniramine alone and against placebo. Each treatment period lasted for 4 weeks. Intensity of...
journal_title：Archives of dermatological research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/BF00412560
更新日期：1984-01-01 00:00:00
abstract：:Human epidermal keratinocytes were grown in culture and the uptake of hematoporphyrin derivatives (HPDs) used in photodynamic therapy was estimated. Keratinocytes loaded with HPDs were irradiated with laser light of 632 nm generated by a helium-neon laser and cell toxicity was determined by the trypan blue exclusion t...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00510086
更新日期：1989-01-01 00:00:00
abstract：:This study was designed to investigate the effects of griseofulvin, 5-fluorocytosine (5-FC), and amphotericin B on pancreatic and gastric secretion after the administration of a single large dose, or repeated small doses for 3 weeks, in male albino rats. The exocrine functions of the main digestive glands were only sl...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00694617
更新日期：1979-10-01 00:00:00
abstract：:This study was designed in order to prove on a large-scale basis the efficacy of oral photochemotherapy (PUVA) in the prevention of polymorphous light eruption (PLE), to work out indication criteria for PUVA treatment of this disease, and to establish a simple method based on anamnestic data to differentiate UVA from ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00417337
更新日期：1983-01-01 00:00:00
abstract：:Myotonic muscular dystrophy (MMD) is caused by an abnormal function of RNA-binding proteins (RBP) resulting in DNA spliceopathy. A case of a patient, with MMD multiple basal and squamous cell carcinomas and dysplastic nevi, is described. The association between MMD and non-melanoma skin cancer has been reported before...
journal_title：Archives of dermatological research
pub_type： 新闻
doi：10.1007/s00403-009-0997-8
更新日期：2010-04-01 00:00:00
abstract：:Studies aimed at understanding the pathology, genetics, and therapeutic response of vitiligo rely on asking a single question about 'physician-diagnosed' vitiligo on surveys to identify subjects for research. However, this type of self-reporting is not sufficient. Our objective was to determine if the patient-administ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-014-1515-1
更新日期：2015-01-01 00:00:00
abstract：:The mechanical properties of the in vivo dermis were measured by means of a torque applied to the skin. The resulting deformation of 2-6 degrees, including the immediate and delayed visco-elastic components, as well as the relaxation were measured, and the raw values corrected for a constant skin thickness. The experi...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00406415
更新日期：1980-01-01 00:00:00
abstract：:Medical innovation is crucial to advancing our field, and physicians have the potential to play a leading role due to their daily patient care experiences. The objective of this study was to evaluate the interest in, and barriers to participating in innovation. Two surveys were conducted; the first cross-sectional sur...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-020-02118-6
更新日期：2020-08-09 00:00:00
abstract：:Exposure to solar radiation is a major cause of environmental human skin damage. The main constituent of solar UV light is UVA radiation (320-400 nm); however, the need for protection against UVA has been marginalized for a long time. As a result, there is still a lack of useful agents for UVA protection. In this stud...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-018-1828-6
更新日期：2018-07-01 00:00:00
abstract：:When determined in vitro, the SPF of certain commercial sunscreen products can be lower than the SPF indicated on the label. The study of the composition of this type of product enabled us to note that the majority contained substances reputed to have anti-inflammatory properties. This effect is shown by inhibiting th...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-012-1257-x
更新日期：2012-12-01 00:00:00
abstract：:A recently described endogenous proteolytic process in pieces of human plantar stratum corneum incubated in vitro has been further studied. This process leads to a decrease in cohesion between the cells that had been facing outwards in vivo. Using two methods, that differed with respect to efficiency, to detach surfac...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00371642
更新日期：1990-01-01 00:00:00
abstract：:Pathological alterations of capillaries, accelerated epidermopoesis, increased rate and extent of metabolism in the psoriatic lesion and first manifestation of psoriasis rarely in the first 20 years of life made us decide to investigate the human growth hormone (STH) in blood serum. In 70 patients suffering from psori...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00408223
更新日期：1981-01-01 00:00:00
abstract：:Costimulation of anti-CD3-triggered proliferative T-cell responses by type I and type IV collagen and fibronectin was studied in 25 patients with psoriasis and 12 healthy subjects. The stimulation index of anti-CD3-mediated responses in the presence of type I collagen was about half that in the controls. Although the ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF01262328
更新日期：1995-01-01 00:00:00
