Abstract:
:Exposure to solar radiation is a major cause of environmental human skin damage. The main constituent of solar UV light is UVA radiation (320-400 nm); however, the need for protection against UVA has been marginalized for a long time. As a result, there is still a lack of useful agents for UVA protection. In this study, the effect of silymarin (SM) and its main constituent silybin (SB) pre-treatment on UVA-stimulated damage to primary human dermal fibroblasts were carried out. The cells were pre-treated for 1 h with SB or SM and then were exposed to UVA light, using a solar simulator. The effect of SB and SM on reactive oxygen species (ROS) and glutathione (GSH) level, caspase-3 activity, single-strand breaks (SSB) formation and protein level of matrix metalloproteinase-1 (MMP-1), heme oxygenase-1 (HO-1), and heat shock protein (HSP70) was evaluated. Treatment with both SM and SB resulted in a reduction in UVA-stimulated ROS generation and SSB production, as well as in the prevention of GSH depletion, a decrease in the activation of caspase-3 and protein level of MMP-1. They also moderately increased HO-1 level and reduced HSP70 level. Our data showed that both SM and SB are non-phototoxic and have UVA-photoprotective potential and could be useful agents for UV-protective dermatological preparations.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Rajnochová Svobodová A,Gabrielová E,Michaelides L,Kosina P,Ryšavá A,Ulrichová J,Zálešák B,Vostálová Jdoi
10.1007/s00403-018-1828-6subject
Has Abstractpub_date
2018-07-01 00:00:00pages
413-424issue
5eissn
0340-3696issn
1432-069Xpii
10.1007/s00403-018-1828-6journal_volume
310pub_type
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