Abstract:
:Inward rectifier potassium currents and calcium-dependent potassium currents have been studied in cultured embryonic mouse motoneurons. Sustained unitary inward rectifier potassium currents were recorded from cell-attached patches and the channel conductance was dependent on external K+ concentration with a value of 25 pS when external K+ was 140 mM. The channel open probability exhibited a sigmoidal dependence on potential with the largest values (near 0.7) at depolarizing patch potentials. Inactivating inward rectifier potassium currents were also recorded in some cell-attached patches following voltage steps to hyperpolarizing potentials with the rate of inactivation faster with larger hyperpolarizing steps. Whole-cell inward rectifier potassium currents increased from an initial level to a steady-state level with hyperpolarizing steps to -120 mV from a holding potential of -60 mV; with larger hyperpolarizing commands the peak currents decayed to the steady-state. The steady-state current-voltage relation exhibited a region of negative slope resistance. External Cs+ (0.5-1 mM) reduced the amplitudes of macroscopic currents and diminished the open times of unitary currents consistent with block of open rectifying channels with an estimated KD for channel block of 1 mM. A large conductance calcium-dependent potassium channel was isolated in inside-out patches with a conductance of 240 pS with symmetrical 140 mM K+ across the patches and a conductance of 110 pS when the external K+ was reduced to 5 mM. With symmetrical K+ the channel open probability exhibited a sigmoid dependence on potential with the largest values, in excess of 0.8, associated with patch depolarization. The dependence of open probability on potential was dependent on the concentrations of internal Ca2+ and external K+. Properties of inward rectifier and calcium-dependent K+ channels, such as the voltage dependence of open probability, are involved in the establishment of cellular excitability in motoneurons. Future studies will be useful to investigate whether channel properties of motoneurons are altered after cell treatment with neurotoxic agents including oxygen radicals or excitotoxic amino acids.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
McLarnon JG,Kim SU,Michikawa M,Xu Rdoi
10.1016/0306-4522(95)90396-osubject
Has Abstractpub_date
1995-01-01 00:00:00pages
139-51issue
1eissn
0306-4522issn
1873-7544pii
0306-4522(95)90396-Ojournal_volume
64pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::Proton magnetic resonance spectroscopy (1H-MRS) can provide noninvasive detection of brain metabolite changes in vivo in Alzheimer's disease (AD). AD is a prevalent neurodegenerative disorder characterized by deposition of β-amyloid peptides (Aβ) in multiple brain regions. Brain-derived neurotrophic factor (BDNF) is a...
journal_title:Neuroscience
pub_type: 杂志文章,收录出版
doi:10.1016/j.neuroscience.2013.04.015
更新日期:2013-08-15 00:00:00
abstract::The blockade of the central nucleus of the amygdala (CeA) with the GABAA receptor agonist muscimol significantly reduces hypertonic NaCl and water intake by sodium-depleted rats. In the present study we investigated the effects of previous injection of losartan, an angiotensin II type-1 (AT1) receptor antagonist, into...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.08.069
更新日期:2015-10-29 00:00:00
abstract::Walking onto a stationary sled previously experienced as moving induces locomotor aftereffects (LAE, or "broken escalator phenomenon"). This particular form of aftereffect can develop after a single adaptation trial and occurs despite subjects being fully aware that the sled will not move. Here, we investigate whether...
journal_title:Neuroscience
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.neuroscience.2010.06.079
更新日期:2010-09-29 00:00:00
abstract::Corticothalamic axons from layer 5 of primary and secondary auditory and visual areas have large terminals that make multiple synaptic contacts on proximal dendrites of relay cells in higher order thalamic nuclei and have been termed "driver" inputs. The corticothalamic cells express mRNA for the presynaptic growth-as...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2005.08.033
更新日期:2005-01-01 00:00:00
abstract::In mice carrying the weaver mutation there is a spontaneous degeneration of dopaminergic neurons that is heterogeneous among cell groups: nigrostriatal neurons are more affected than mesolimbic neurons, while involvement of the mesocortical system is controversial. We questioned whether the pattern of cell loss in mes...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)90232-1
更新日期:1994-07-01 00:00:00
abstract::Electric current has been used for epilepsy treatment by targeting specific neural circuitries. Despite its success, direct contact between the electrode and tissue could cause side effects including pain, inflammation, and adverse biological reactions. Magnetic stimulation overcomes these limitations by offering adva...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2020.02.018
更新日期:2020-04-15 00:00:00
abstract::Probenecid has been used for decades to treat gout, and recent studies have revealed it is also a specific inhibitor of the pannexin-1 channel. It has been reported that the pannexin-1 channel is involved in ischemic injury. Here, we investigated the neuroprotective effect and the possible mechanisms of action of prob...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.05.070
更新日期:2015-08-20 00:00:00
abstract::Stroke-prone spontaneously hypertensive rats (SHRSP/Izm) develop severe hypertension, and more than 95% of them die of cerebral stroke. Hypoxic stimulation followed by oxygen reperfusion induces neuronal damage in both normotensive Wistar Kyoto/Izm (WKY/Izm) and SHRSP/Izm rats, and the percentage of neurons that under...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2010.07.013
更新日期:2010-09-29 00:00:00
abstract::Perceiving the sensory consequences of our actions with a delay alters the interpretation of these afferent signals and impacts motor learning. For reaching movements, delayed visual feedback of hand position reduces the rate and extent of visuomotor adaptation, but substantial adaptation still occurs. Moreover, the d...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2020.07.041
更新日期:2020-10-15 00:00:00
abstract::Whole-cell recordings were made from neurons of the rat locus coeruleus in a tissue slice removed from rat brain. Substance P caused an inward current in cells voltage-clamped at -60 mV. The effect of substance P was concentration-dependent (30 nM-3 microM) and was mimicked by similar concentrations of substance K and...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90428-5
更新日期:1992-09-01 00:00:00
abstract::The location of sympathetic preganglionic neurons (SPN) in the spinal cord of the reeler mouse mutant is abnormal. Instead of their normal location in the intermediolateral column, the majority of SPN in the reeler cluster around the central canal. To determine whether ectopically located SPN in the reeler form approp...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(02)00945-4
更新日期:2003-01-01 00:00:00
abstract::Spontaneous activity in medial vestibular nucleus (MVN) neurons is modulated by synaptic inputs. These inputs are crucial for maintaining gaze and posture and contribute to vestibular compensation after lesions of peripheral vestibular organs. We investigated how chronically attenuated glycinergic input affects excita...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.06.040
更新日期:2010-09-29 00:00:00
abstract::Free intracellular calcium concentration ([Ca2+]in) was recorded at 22 degrees C by means of Indo-1 or Fura-2 single-cell microfluorometry in cultured dorsal root ganglion neurons obtained from neonatal rats. The resting [Ca2+]in in dorsal root ganglion neurons was 73 +/- 21 nM (mean +/- S.D., n = 94). Fast applicatio...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(93)90029-f
更新日期:1993-12-01 00:00:00
abstract::More dendritic spine synapses occur on mature neurons in hippocampal slices by 2 h of incubation in vitro, than in perfusion-fixed hippocampus. What conditions initiate this spinogenesis and how rapidly do the spines begin to proliferate on mature neurons? To address these questions, CA1 field of the hippocampus neuro...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2004.04.053
更新日期:2004-01-01 00:00:00
abstract::Retinitis pigmentosa (RP) is a heterogeneous group of retinal degenerative diseases in which the final pathological feature is photoreceptor cell apoptosis. Currently, the pathogenesis of RP remains poorly understood and therapeutics are ineffective. 17β-Oestradiol (βE2) is universally acknowledged as a neuroprotectiv...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2020.11.010
更新日期:2021-01-01 00:00:00
abstract::4-Aminopyridine (4-AP) is suggested to improve symptomatology of spinal injury patients because it may facilitate neuromuscular transmission, spinal impulse flow and the operation of the locomotor central pattern generator (CPG). Since 4-AP can also induce repetitive discharges from dorsal root afferents, this phenome...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2005.02.012
更新日期:2005-01-01 00:00:00
abstract::In amyotrophic lateral sclerosis (ALS) there is a selective degeneration of motor neurons leading to muscle paralysis and death. The mechanism underlying cell demise in ALS is not fully understood, but involves the activation of different proteolytic enzymes, including the caspase family of cysteine proteases. We have...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2006.07.048
更新日期:2006-12-01 00:00:00
abstract::Prolonged stress triggers neuroinflammation, which plays a significant role in the development of depression; however, stressed people do not always suffer from depression because of individual differences in stress vulnerability. Negative cognitive bias (NCB) toward pessimistic judgment often underlies depressive epi...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2020.07.023
更新日期:2020-09-01 00:00:00
abstract::In spite of the existence of pedunculopontine tegmental nucleus (PPTg) projections to cerebellar nuclei, their nature and functional role is unknown. These fibers may play a crucial role in postural control and may be involved in the beneficial effects induced by deep-brain stimulation (DBS) of brainstem structures in...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.12.055
更新日期:2016-03-11 00:00:00
abstract::Three closely-linked clusters of protocadherin (Pcdh) genes (alpha, beta, and gamma) encoding more than 50 distinct mRNAs have been identified in humans and mice, and proposed to play important roles in neuronal connectivity in the CNS. The human and mouse Pcdh alpha and gamma clusters each span a region of about 300 ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2006.10.011
更新日期:2007-01-19 00:00:00
abstract::The metabotropic glutamate receptor 4 is highly expressed presynaptically on thalamocortical neurons that are involved in the pathogenesis of generalized absence seizures. Mutant mice devoid of metabotropic glutamate receptor 4 are completely resistant to absence seizures induced by low doses of GABA type A receptor a...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2005.05.033
更新日期:2005-01-01 00:00:00
abstract::Peripheral diabetic neuropathy (PDN) manifests in 50-60% of type I and II diabetic patients and is the major cause of limb amputation. Adequate therapy for PDN is a current challenge. There are evidences that the activation of the P2X4 receptor (P2X4R) expressed on microglial cells of the central nervous system takes ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2018.12.003
更新日期:2019-02-01 00:00:00
abstract::It has been half a century since brain volume enlargement was first reported in animals reared in an enriched environment (EE). As EE animals show improved memory task performance, exposure to EE has been a useful model system for studying the effects of experience on brain plasticity. We review EE-induced neural chan...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2014.09.031
更新日期:2014-11-07 00:00:00
abstract::Alzheimer's disease (AD), the most prominent form of dementia in elderly, is a yet incurable degenerative neurological illness characterized by memory loss. Here, we used an AD rat model to investigate the in vivo efficacy of caprospinol, a disease-modifying steroid developed on the concept that reduced synthesis of 2...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.10.033
更新日期:2010-01-20 00:00:00
abstract::Non-invasive criteria determining the progress of brain healing are especially important in aging, providing a case-specific therapeutic strategy in populations with dysregulated neurorepair mechanisms. We hypothesized that temporal evolution of magnetic resonance imaging (MRI) of T2 tissue relaxation values correlate...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.09.028
更新日期:2014-12-26 00:00:00
abstract::Nicotinic cholinergic receptors in the ventral tegmental area are required for the accumbal dopamine response to systemic nicotine. In contrast, the role of nicotinic receptors located within the nucleus accumbens itself has not been clarified for systemically administered nicotine. In the present study, in vivo micro...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00371-7
更新日期:2000-01-01 00:00:00
abstract::This study has investigated the role of hypothalamic and amygdalar type-1 cannabinoid (CB1) receptors in the emotional and neuroendocrine responses to stress. To do so, we used the Cre/loxP system to generate conditional mutant mice lacking the CB1 gene in neurons expressing the transcription factor single-minded 1 (S...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.08.049
更新日期:2012-03-01 00:00:00
abstract::Arising from studies on the amnesia that follows site-specific physical or chemical lesions, the acquisition and consolidation of certain behavioral tasks has been demonstrated to be associated with different hippocampal subregions. Although not absolute, spatial learning is reliant on the dorsal region of the hippoca...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.01.020
更新日期:2012-05-03 00:00:00
abstract::Whereas activation of α1-adrenoceptors (α1-ARs) modulates glutamatergic transmission, the roles of α1-ARs in GABAergic transmission in the medial prefrontal cortex (mPFC) are elusive. Here, we examined the effects of the α1-AR agonist phenylephrine (Phe) on GABAergic transmission onto pyramidal neurons in the deep lay...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.04.070
更新日期:2015-08-06 00:00:00
abstract::The host response to neural injury, which can include axonal sprouting and synaptic reorganization is likely to be under tight genetic regulatory control at the level of the genome and may be implicated in epileptogenesis. Despite its importance, however, the molecular basis of synaptic reorganization is unclear. We h...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2004.09.064
更新日期:2005-01-01 00:00:00