Abstract:
:Free intracellular calcium concentration ([Ca2+]in) was recorded at 22 degrees C by means of Indo-1 or Fura-2 single-cell microfluorometry in cultured dorsal root ganglion neurons obtained from neonatal rats. The resting [Ca2+]in in dorsal root ganglion neurons was 73 +/- 21 nM (mean +/- S.D., n = 94). Fast application of 20 mM caffeine evoked [Ca2+]in transient which reached a peak of 269 +/- 64 nM within 5.9 +/- 1.1 s. After reaching the peak the [Ca2+]in level started to decline in the presence of caffeine and for 87.2 +/- 10.6 s cytoplasmic calcium returned to an initial resting value. In 40% of neurons tested [Ca2+]in decreased to subresting levels following the washout of caffeine (the so-called post-caffeine undershoot). On average, the undershoot level was 19 +/- 2.5 nM below the resting [Ca2+]in value. Prolonged exposure of caffeine depleted the caffeine-sensitive stores of releasable Ca2+; the degree of this depletion depended on caffeine concentration. The depletion of the caffeine-sensitive internal stores to some extent was linked to calcium extrusion via La(3+)-sensitive plasmalemmal Ca(2+)-ATPases. The stores could be partially refilled by the uptake of cytoplasmic Ca2+, but the complete recovery of releasable Ca2+ content of the caffeine-sensitive pools required the additional calcium entry via voltage-operated calcium channels. Caffeine-evoked [Ca2+]in transients were effectively blocked by 10 microM ryanodine, 5 mM procaine, 10 microM dantrolene or 0.5 mM Ba2+, thus sharing the basic properties of the Ca(2+)-induced-Ca2+ release from endoplasmic reticulum. Pharmacological manipulation with caffeine-sensitive stores interfered with the depolarization-induced [Ca2+]in transients. In the presence of low caffeine concentration (0.5-1 mM) in the extracellular solution the rate of rise of the depolarization-triggered [Ca2+]in transients significantly increased (by a factor 2.15 +/- 0.29) suggesting the occurrence of Ca(2+)-induced Ca2+ release. When the caffeine-sensitive stores were emptied by prolonged application of caffeine, the amplitude and the rate of rise of the depolarization-induced [Ca2+]in transients were decreased. These facts suggest the involvement of internal caffeine-sensitive calcium stores in the generation of calcium signal in sensory neurons.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Usachev Y,Shmigol A,Pronchuk N,Kostyuk P,Verkhratsky Adoi
10.1016/0306-4522(93)90029-fsubject
Has Abstractpub_date
1993-12-01 00:00:00pages
845-59issue
3eissn
0306-4522issn
1873-7544pii
0306-4522(93)90029-Fjournal_volume
57pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::Mature neurons contain two distinct regulated secretory pathways, characterized electron microscopically by so-called large dense core vesicles and small synaptic vesicles, respectively. Each vesicle type is characterized by vesicle-specific proteins, such as the granins (chromogranins/secretogranins) for the matrix o...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90044-3
更新日期:1992-01-01 00:00:00
abstract::The neuronal uptake and laminar distribution of cortically injected tritium-labeled gamma-aminobutyrate (GABA), aspartic acid, glutamate and glycine was examined in the prestriate cortex of squirrel monkeys. The intent of this investigation was not to examine the role of these amino acids as neurotransmitters, but to ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(87)90343-5
更新日期:1987-08-01 00:00:00
abstract::The adverse consequences of early-life sleep deprivation on mental health are well recognized, yet many aspects remain unknown, therefore, animal studies can offer useful insights. Male Sprague-Dawley rats at postnatal day (PND) 19 were subjected to sleep deprivation (SD) for 14 days (6-8 hours/day). Control (CON) rat...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2019.02.021
更新日期:2019-05-15 00:00:00
abstract::Alzheimer's Disease is the leading neurodegenerative cause of dementia. The pathogenesis is not clearly understood yet, is believed to be the complex interaction between genetic and environmental factors. Consequently vascular risk factors and Apolipoprotein E genotyping are increasingly gaining importance. This study...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2016.03.051
更新日期:2016-06-14 00:00:00
abstract::Curiosity surrounding the physiological relevance of neural insulin signaling has gradually developed since the discovery that nervous tissue contains both the hormone and its receptor. Similar to other receptor tyrosine kinases, ligand interaction with the insulin receptor (IR) activates a variety of intracellular si...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2006.07.055
更新日期:2006-11-17 00:00:00
abstract::Organotypic cultures of fetal or early postnatal striatum were used to assess striatal patch formation and maintenance in the presence or absence of dopaminergic and glutamatergic influences. Vibratome-cut slices of the striatum prepared from embryonic day 19 to postnatal day 4 rat pups were maintained in static cultu...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00535-2
更新日期:2001-01-01 00:00:00
abstract::Horizontal and amacrine cells in the isolated carp retina were impaled with micropipette electrode, identified by their characteristic light responses, and injected iontophoretically with markers for morphological study. Both Lucifer Yellow CH and biocytin were injected simultaneously. Lucifer Yellow was seen by its o...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)90112-0
更新日期:1994-03-01 00:00:00
abstract::Granulocyte-colony stimulating factor (G-CSF) is a growth factor that regulates proliferation, differentiation and survival of hematopoietic progenitor cells. There is growing evidence to suggest that G-CSF exerts a powerful neuroprotective effect in different neurological disorders. However, it has remained to be elu...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.09.045
更新日期:2009-12-29 00:00:00
abstract::The electrophysiological, biochemical and behavioral responses produced by administration of haloperidol were studied in intact rats and in rats with 6-hydroxydopamine-induced partial lesions of the nigrostriatal dopamine pathway. In both control rats and rates tested four to 10 days postlesion, the electrophysiologic...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90168-2
更新日期:1992-01-01 00:00:00
abstract::Developmental changes in ganglioside levels and patterns were investigated in neuronal cells dissociated from 17-day-old fetal rat hemispheres for up to 7 days of culture. Increases in ganglioside contents and the onset of GM3 synthesis, which is associated with proliferation of glial cells, were observed as the neuro...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(91)90207-5
更新日期:1991-01-01 00:00:00
abstract::The relationship between discharge rates of locus coeruleus noradrenergic neurons and rates of norepinephrine release was examined in the anesthetized rat. Neuronal discharge rates of locus coeruleus neurons were altered and quantified using a combined recording-infusion probe. Peri-locus coeruleus infusions of either...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(99)00276-6
更新日期:1999-01-01 00:00:00
abstract::Alzheimer's disease (AD) is a debilitating disease and the most common cause of dementia. As the world population ages even modest advances in therapies and preventative strategies would be of benefit. The specific physiological function of the amyloid precursor protein (APP) remains unclear despite strong genetic and...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2019.10.006
更新日期:2020-01-01 00:00:00
abstract::Previous studies show that most short thoracic propriospinal (TPS; T5-T7) and long descending propriospinal tract (LDPT; C4-C6) neurons are lost following low-thoracic spinal cord contusion injury (cSCI), as assessed by retrograde labeling with fluorogold (FG). Gene microarray and terminal deoxynucleotidyl transferase...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.05.052
更新日期:2011-10-27 00:00:00
abstract::The main action of organophosphorous insecticides is generally believed to be the inhibition of acetylcholinesterase (AChE). However, these compounds also inhibit many other enzymes, any of which may play a role in their toxicity. We tested the neurotoxic mechanism of two organophosphorous insecticides, chlorpyrifos a...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.01.025
更新日期:2010-03-31 00:00:00
abstract::The expression of inducible and constitutive heme oxygenase and biliverdin reductase was studied in normal and cultured peripheral ganglia from adult rats, using immunocytochemistry and in situ hybridization. Dramatic changes were induced by one to two days' culturing of dorsal root ganglia, nodose ganglia, otic gangl...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(99)00466-2
更新日期:2000-01-01 00:00:00
abstract::We investigated the effects of intracerebroventricular injection of angiotensin II on neuronal immediate early gene-encoded protein synthesis in the brain of conscious rats. The expression of seven immediate early gene-encoded transcription factors (c-Fos, FosB, c-Jun, JunB, JunD, Krox-20 (Egr-2) and Krox-24 (NGFI-A, ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)00482-k
更新日期:1995-03-01 00:00:00
abstract::After loading cultured rat hippocampal neurons with teh acetoxymethyl ester of the Ca2+ buffer BAPTA, or its dimethyl analogue DMB, the magnitudes of transient (20-25 s) depolarization- or excitatory amino acid-induced Ca2+ responses were reduced, as were the rates of increase and recovery of [Ca2+]i. In contrast, dur...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(97)00162-0
更新日期:1997-12-01 00:00:00
abstract::At hippocampal Schaffer collateral-CA1 synapses, activation of beta-adrenergic receptors and adenylyl cyclase increases transmitter release. However, this effect is transient, which is in contrast to that seen at mossy fiber-CA3 synapses, where activation of cyclic-AMP-dependent protein kinase results in long-lasting ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00200-0
更新日期:1999-01-01 00:00:00
abstract::The sleep spindle rhythm of thalamic origin (7-14 Hz) displays widespread synchronization among thalamic nuclei and over most of the neocortex. The mechanisms which mediate such global synchrony are not yet well understood. Here, we theoretically address the hypothesis of Steriade and colleagues that the reticularis t...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(93)90474-t
更新日期:1993-04-01 00:00:00
abstract::Multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE), are crucially dependent on the invasion of activated autoreactive lymphocytes and blood macrophages into the central nervous system (CNS). Proinflammatory mononuclear cells and activated local microglia mediate inflammation, demy...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.06.070
更新日期:2014-09-26 00:00:00
abstract::In this study, we examined the prevalence and distribution of NMDA receptor subunits within crude synaptic membranes derived from the brains of mice lacking methyl CpG binding protein 2 (MeCP2). Our results show that the distribution of NMDA receptor subunits within the detergent soluble, detergent resistant, and post...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.03.065
更新日期:2010-07-14 00:00:00
abstract::This study evaluated Fos-like immunoreactivity in lumbar spinal cord neurons following intense cold stimulation and then the modifications induced by opioid administration. Under urethane anaesthesia, the rat's right foot was stimulated by holding it in a regulated temperature bath at 15, 10, 0, -10, -15, -17.5 or -20...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)90609-2
更新日期:1994-03-01 00:00:00
abstract::Neonatal posterior cingulate cortex lesions spare the spatial deficits that characterize adult lesions. The present experiments examined the possibility that the anterior cingulate cortex mediates the spared spatial behavior. Rats were given bilateral lesions of the posterior cingulate cortex or anterior plus posterio...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(03)00295-1
更新日期:2003-01-01 00:00:00
abstract::Dopamine receptors (DARs) in the nucleus accumbens (NAc) are critical for cocaine's actions but the nature of adaptations in DAR function after repeated cocaine exposure remains controversial. This may be due in part to the fact that different methods used in previous studies measured different DAR pools. In the prese...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.04.056
更新日期:2010-08-11 00:00:00
abstract::The 5-HT(2A) serotonin receptor represents an important molecular target for atypical antipsychotic drugs and for most hallucinogens. In the mammalian cerebral cortex, 5-HT(2A) receptors are enriched in pyramidal neurons, within which 5-HT(2A) receptors are preferentially sorted to the apical dendrites. In primary cor...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(03)00589-x
更新日期:2003-01-01 00:00:00
abstract::Endopeptidase-24.11, which is identical with the common acute lymphoblastic leukemia antigen (CALLA), is a cell surface zinc metalloprotease that has the ability to hydrolyse a variety of physiologically active peptides. Interest in this enzyme is based on the view that it may play a role in the regulation of peptide ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90382-c
更新日期:1992-09-01 00:00:00
abstract::Previous studies found that prostaglandins in skeletal muscle play a role in evoking the exercise pressor reflex; however the role played by prostaglandins in the spinal transmission of the reflex is not known. We determined, therefore, whether or not spinal blockade of cyclooxygenase (COX) activity and/or spinal bloc...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.06.061
更新日期:2014-09-26 00:00:00
abstract::Chronic stress and spatial training have been proposed to affect hippocampal structure and function in opposite ways. Previous morphological studies that addressed structural changes after chronic restraint stress and spatial training were based on two-dimensional morphometry which does not allow a complete morphometr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2004.10.031
更新日期:2005-01-01 00:00:00
abstract::Animals change their biological activities depending on their nutritional state. Reproductive functions, including sexual behavior, are suppressed under low-energy conditions; however, the underlying neuronal mechanism is poorly understood. Neuropeptide Y (NPY) is an orexigenic molecule released in response to low-ene...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2016.01.069
更新日期:2016-04-21 00:00:00
abstract::Contraversive turning movements of the head were elicited in alert cats by unilateral electrical stimulation of the entopeduncular nucleus. To determine the relative functional importance of ascending and descending pathways activated by the stimulation, the animals were submitted to thalamocortical or midbrain lesion...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(83)90090-8
更新日期:1983-09-01 00:00:00