Developmental neurotrophin expression in slice cultures of rat hippocampus.

Abstract:

:Using reverse transcription in combination with the polymerase chain reaction, the developmental expression of neurotrophins in organotypic slice cultures of rat hippocampus was investigated. Nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) mRNA levels after different time periods in vitro were compared with equivalent developmental stages in vivo. Our results show that neurotrophin expression occurs in hippocampal slice cultures with a similar time course as observed in the developing hippocampus in vivo. Thus, the development of neurotrophin expression in the hippocampus does not seem to be dependent on specific extrinsic afferents.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Förster E,Otten U,Frotscher M

doi

10.1016/0304-3940(93)90711-s

subject

Has Abstract

pub_date

1993-06-11 00:00:00

pages

216-9

issue

2

eissn

0304-3940

issn

1872-7972

pii

0304-3940(93)90711-S

journal_volume

155

pub_type

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