Abstract：
:Basophils are implicated in the pathogenesis of the late-phase allergic reaction, but the mechanisms by which circulating basophils adhere to vascular endothelium and migrate to lesional sites remain unclear. In order to assess the biological similarity of the basophilic cell line KU-812 to normal human basophils, we have compared the adhesion response of this cell line and normal basophils, following challenge with interleukin-8 (IL-8) and RANTES. We demonstrate here that IL-8 and RANTES are able to stimulate the adherence of the basophilic cell line, KU-812, to cytokine-activated human umbilical vein endothelium (HUVEC). The chemokine-induced increase in adhesion was dose-related and was maximal after prior priming with IL-5. The stimulation of adhesion was partially inhibited by co-incubation with anti-CD18 and anti-CD11c antibodies and antibodies to the beta 1-integrins. In comparison, the chemokine-induced adhesion of normal human basophils was only inhibited by the beta 2-integrins. These chemokines were also able to induce the migration of KU-812 in a dose-dependent manner, but only after prior treatment with phorbol myristate acetate (PMA) or IL-5. In all cases tested, IL-8 was more potent and efficacious than RANTES. We conclude from these studies that these members of the chemokine superfamily may play an important role in the recruitment of reactive leukocytes in allergic inflammation, by stimulating their adhesion and subsequent migration from the vasculature into the inflammatory sites. However, it is apparent that KU-812 is not an adequate substitute for normal human basophils in order to investigate chemokine biology.
journal_name
Immunologyjournal_title
Immunologyauthors
Bacon KB,Flores-Romo L,Aubry JP,Wells TN,Power CAsubject
Has Abstractpub_date
1994-07-01 00:00:00pages
473-81issue
3eissn
0019-2805issn
1365-2567journal_volume
82pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract：:The nature of the Fc receptors for IgG subclasses displayed on the epithelium of the small intestine in neonatal rats was examined by comparing results gained from 125I-IgG binding studies using isolated enterocyte brush borders, and from experiments where in vivo transmission from the gut to the bloodstream was quant...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1985-09-01 00:00:00
abstract：:Immununoelectron microscopy of human peripheral blood mononuclear cells enriched for the presence of antigen-presenting dendritic cells (DC) has revealed two morphologically distinct cell types both expressing DR and DQ major histocompatibility complex (MHC) class II antigens but lacking T, B, natural killer (NK) and ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
abstract：:Dendritic cells (DC) are powerful inducers of primary T-cell responses, but their role in secondary responses has not been extensively analysed. Here, we address the role of two DC subsets derived from human CD16(+) (16(+) mDC) or CD16(-) (16(-) mDC) monocytes on the reactivation of memory responses. CD4(+) CD45RA(-) ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2007.02650.x
更新日期：2007-11-01 00:00:00
abstract：:Cancer immune surveillance is considered to be an important host protection process to inhibit carcinogenesis and to maintain cellular homeostasis. In the interaction of host and tumour cells, three essential phases have been proposed: elimination, equilibrium and escape, which are designated the 'three E's'. Several ...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2567.2007.02587.x
更新日期：2007-05-01 00:00:00
abstract：:Intestinal inflammatory disease or infection often results in the loss of the epithelial layer as a result mainly of the action of proteases, including the leucocyte serine proteinases (neutrophil elastase), lysosomal cathepsins and the matrix metalloproteinases from recruited inflammatory cells. Previous studies have...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.0019-2805.2001.01352.x
更新日期：2002-01-01 00:00:00
abstract：:The L2C leukaemia is a B-lymphocytic neoplasm of strain 2 guinea-pigs, maintained by passaging in vivo. It synthesizes mu and lambda immunoglobulin chains. These combines to form monomeric (7S) IgM molecules which are inserted into the plasma membranes. From here they are shed as monomeric IgM and as a species of high...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1978-05-01 00:00:00
abstract：:After a primary infection of congenitally athymic nu/nu mice with Strongyloides ratti, worms were not expelled and the number of intestinal mucosal mast cells (MMC) remained at a low level. When S. ratti-infected nu/nu mice were treated by repetitive injections of semi-purified IL-3 from Day 5 to Day 10 post-infection...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
abstract：:The soluble cytoplasmic fraction of an homogenate from peritoneal rat mast cells, demonstrated a considerable amount of catalytic activity which promotes the transfer of phosphate from creatine phosphate to ADP. The plasma membrane, mitochondrial and microsomal fractions show negligible amounts of the catalyst. Enzyme...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1980-03-01 00:00:00
abstract：:The intestinal lamina propria (LP) contains antigen-presenting cells with features of dendritic cells and macrophages, collectively referred to as mononuclear phagocytes (MNPs). Association of MNPs with the epithelium is thought to play an important role in multiple facets of intestinal immunity including imprinting M...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12801
更新日期：2017-12-01 00:00:00
abstract：:Expression of Fc gamma receptors on human placental trophoblast was investigated by immunostaining and immunoblotting using a panel of Fc gamma receptor monoclonal antibodies (mAb). Fc gamma receptors typical of other cell types were not detected on syncytiotrophoblast in term placentae when transplacental IgG transpo...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：:The Ager assay was adapted to a porcine lymphocyte-rat high endothelial cell (HEC) system. Using this in vitro assay, the role of porcine CD44 in lymphocyte binding to HEC was examined. The results show that the presence of soluble CD44 molecules did not inhibit the binding of porcine lymphocytes to the cultured rat H...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-07-01 00:00:00
abstract：:Crry (CR1-related gene/protein) is a rodent complement regulator that inhibits C3 convertases. CD59 is a conserved protein inhibitor active towards C8 and C9. We have previously produced rat Crry as a recombinant soluble (rs) protein in Pichia pastoris. In this study we produced functionally active rat rsCD59 and a ch...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2000.00945.x
更新日期：2000-01-01 00:00:00
abstract：:We have previously shown that chlorpromazine (CPZ) inhibits tumour necrosis factor (TNF) production and protects against endotoxic shock in mice. In this paper we investigated the effect of pretreatment with CPZ, 4 mg/kg i.p. 30 min before, compared with dexamethasone (DEX; 3 mg/kg) on the induction of other endotoxin...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:Human autologous-rosette-forming T cells (Tar cells) have many of the characteristics of post-thymic precursor cells. Thus, they bind to sheep erythrocytes but have neither receptors for the Fc portion of IgG nor for that of IgM. They include a subpopulation that binds peanut agglutinin which suggests that they are im...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:The immunosuppressive activity of serum from PVG rats following orthotopic transplantation of DA liver has been examined in vitro. Liver grafts in this combination are never rejected, but induce a state of specific transplantation tolerance in the recipient. Serum from such tolerant animals was able to inhibit prolife...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1987-08-01 00:00:00
abstract：:The IgE immune response was studied in female athymic, nude (Lewis rnu/rnu) and euthymic (Lewis +/+) rats infected with the nematode Nippostrongylus brasiliensis. During the course of the infection, serum IgE levels were followed using an enzyme-linked immunosorbent assay technique (ELISA), while the surface expressio...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:Self-reactive clones, estimated by anti-V beta monoclonal antibodies (mAb) in conjunction with the Mls system, are confined to a population of intermediate (int) T-cell receptor (TCR) (or CD3) cells (i.e. TCRint cells), but are not found among TCRhigh cells. The next questions to be answered are whether autologous kil...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1997.00240.x
更新日期：1997-06-01 00:00:00
abstract：:The defined antigen substrate spheres (DASS) system was employed for analysing reactions between solid state antibody or antigen and soluble immune complexes. Sepharose beads covalently coupled with ovalbumin were used to represent tumour cells and beads coupled with antibody against ovalbumin were used to represent a...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1975-06-01 00:00:00
abstract：:The influence of changes of the cation-transporting activity of the membrane Na,K ATP-ase of human peripheral blood lymphocytes on their ability to form 'early' and 'late' rosettes with SRBC was investigated. It was shown that inhibition of the Na,K ATP-ase-dependent cation transport through the lymphocyte membrane by...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
abstract：:The purpose of this study was to investigate the effect of cyclophosphamide (CY) and 6-mercaptopurine (6-MP) on the production of the two IgG antibody subclasses in guinea-pigs. Following the intradermal application of dinitrophenylated bovine gamma globulin (DNP20-BGG) emulsified in Freund's incomplete adjuvant (FIA)...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1981-09-01 00:00:00
abstract：:Spleen cells from LAF1 mice primed with photo-oxidized antigen B exhibit helper T-cell activity when assayed in an adoptive transfer system with spleen cells from mice primed with timothy pollen extract and treated with anti-thy 1.2 serum and complement. These helper cells, when placed in culture with antigen B, secre...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1981-10-01 00:00:00
abstract：:We have used a number of in vitro and in vivo techniques to identify the molecules that can bind to the cytoplasmic tail of the Ly49A receptor. Affinity chromatography using peptides corresponding to the N-terminal 18 amino acids of Ly49A allowed the recovery of a number of proteins that bound preferentially to the ty...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1999.00825.x
更新日期：1999-08-01 00:00:00
abstract：:Antibody plaque-forming cells (FC) to the somatic antigens of Vibrio cholerae were enumerated in the spleen of mice after primary and secondary immunization with a heat-killed vaccine prepared from the vibrios. Immunocytes releasing both high efficiency IgM and low efficiency IgG antibody were readily detected using a...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1975-08-01 00:00:00
abstract：:Cytotoxic T lymphocytes (CTL) may play an important role in the control of human papillomavirus (HPV)-induced anogenital neoplasias, but have been difficult to study owing to the difficulty in obtaining sufficient quantities of infectious virus. To address this we have stimulated human HPV-specific CTL in vitro using ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:Glucosamine (GlcN) has been reported to possess several biomedical properties, and currently a great deal of attention has been focused on improving the functional properties of GlcN for different applications. Therefore, this study was conducted to introduce a carboxybutyryl functional group to GlcN and to find out t...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2007.02683.x
更新日期：2008-03-01 00:00:00
abstract：:Bone marrow cells from mice were cultured in vitro in the presence of interleukin-3 (IL-3). After 2 weeks, the majority of the cells differentiated towards mast cells as judged by morphological and biochemical criteria. When populations of 99% IL-3-dependent mast cells (IL-3 MC) were tested for their anti-tumour activ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
abstract：:Humans and other mammals coexist with a diverse array of microbes colonizing the intestine, termed the microflora. The relationship is symbiotic, with the microbes benefiting from a stable environment and nutrient supply, and the host gaining competitive exclusion of pathogens and continuously maintenance of the gut i...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2007.02687.x
更新日期：2008-02-01 00:00:00
abstract：:Free malarial merozoites isolated from in vitro cultures of P. knowlesi and emulsified with Freund's complete (FCA) or incomplete (FIA) adjuvant were used to vaccinate twelve Rhesus monkeys against the uniformly lethal infection caused by P. knowlesi. Initial challenge of six monkeys with the same parasite variant as ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1975-08-01 00:00:00
abstract：:Bovine respiratory syncytial virus (RSV) is a major cause of respiratory disease in young calves. Recent studies in calves, in which different T-cell subsets were depleted, have shown that CD8+ T cells play a central role in recovery from RSV infection. The present study demonstrates that RSV-specific, major histocomp...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1996.d01-667.x
更新日期：1996-07-01 00:00:00
abstract：:Variation in the sequence of T-cell epitopes between dengue virus (DENV) serotypes is believed to alter memory T-cell responses during second heterologous infections. We identified a highly conserved, novel, HLA-B57-restricted epitope on the DENV NS1 protein. We predicted higher frequencies of B57-NS1(26-34) -specific...
journal_title：Immunology
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1111/imm.12161
更新日期：2014-01-01 00:00:00
