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Protection by atropine of the inhibition caused by Triton X-100 on central muscarinic receptors.

Abstract：

:Synaptosomal membranes from cat cerebral cortex were labelled with [3H]-quinuclidinyl benzylate ([3H]QNB). There was shown to be a single type of binding site with Kd = 0.34 nM, Bmax = 2.2 nmol/g protein and Hill No. = 1.01. Triton X-100 at 5 x 10(-4)% inhibited the specific binding of [3H]QNB and the inhibition was almost complete at 10(-2)%. Treatment with 2.5 x 10(-6) M atropine, followed by centrifugation washings protected the receptor site from the inhibitory action of the detergent. The protection afforded by other cholinergic drugs was less effective. The use of this technique has confirmed the results of our previous work on the possible pre- and postsynaptic location of central muscarinic receptors. These findings open the possibility for protection of other detergent-sensitive receptors and for their isolation and purification as well-defined macromolecules.

journal_name

Eur J Pharmacol

journal_title

European journal of pharmacology

authors

Aguilar JS,Criado M,De Robertis E

doi

10.1016/0014-2999(80)90252-6

subject

Has Abstract

pub_date

1980-05-16 00:00:00

pages

251-7

issue

4

eissn

0014-2999

issn

1879-0712

pii

0014-2999(80)90252-6

journal_volume

63

pub_type

杂志文章

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