Abstract:
:The binding of eight fluorescence-labeled lectins to the photoreceptors of the monkey retina was investigated using a post-embedding staining method. Concanavalin A (specific for mannosyl and glucosyl residues) bound to the outer and inner segments of both rods and cones, while the degree of staining was more intense in the rods. The rod outer segments showed patchy fluorescence and the proximal portions of the inner segments were diffusely stained. Wheat germ agglutinin (specific for sialyl and N-acetylglucosaminyl residues) bound preferentially to the surface of the outer and inner segments of both rods and cones. Ricinum communis agglutinin-1 (specific for galactosyl residues) stained the rod outer segments in patches, particularly strongly in the region dividing the outer and inner segments. The cones were also stained, although faintly, in the same pattern as the rods. The distal halves of the rods and cones showed diffuse weak staining and their proximal halves stained spotty. Peanut agglutinin (specific for Gal beta 1 leads to 3GalNAc sequence) bound preferentially to the cones and only scarcely to the rods. The external surface of both outer and inner segments of cones were uniformly stained; the interior of the cone outer segments was also stained, while the interior of the inner segments was not. Two lectins specific for fucosyl residues, namely, Ulexeuropaeus agglutinin-1 and Lotustetragonolobus agglutinin, bound diffusely to the distal halves of the inner segments of both rods and cones. Lectins reacting with N-acetyl-galactosamine residue, i.e. Dolichos biflorus agglutinin and soybean agglutinin, bound weakly to the distal portions of rods and cones.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Uehara F,Sameshima M,Muramatsu T,Ohba Ndoi
10.1016/0014-4835(83)90094-5subject
Has Abstractpub_date
1983-01-01 00:00:00pages
113-23issue
1eissn
0014-4835issn
1096-0007pii
0014-4835(83)90094-5journal_volume
36pub_type
杂志文章abstract::The aim of this study was to examine the murine subbasal nerve fibre plexus (SNP) regeneration altered by surgical dissection. Investigations in the mouse model addressed the regeneration capabilities of the SNP, and the influence of local ciliary neurotrophic factor (CNTF) application on the regeneration process. In ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.12.015
更新日期:2014-03-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2008.03.013
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.05.015
更新日期:2013-08-01 00:00:00
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journal_title:Experimental eye research
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doi:10.1016/0014-4835(89)90059-6
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pub_type: 杂志文章
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1085
更新日期:1993-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(85)90147-2
更新日期:1985-03-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2019-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.05.011
更新日期:2004-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.07.004
更新日期:2014-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.04.015
更新日期:2019-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1999.0703
更新日期:1999-09-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2012.11.019
更新日期:2013-09-01 00:00:00
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2015.02.012
更新日期:2015-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.04.001
更新日期:2014-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.10.020
更新日期:2014-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2012.07.007
更新日期:2012-09-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.01.018
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.11.013
更新日期:2006-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.02.005
更新日期:2007-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(02)00311-1
更新日期:2003-03-01 00:00:00
abstract::The binding of monoclonal and polyclonal antibodies to glial fibrillary acidic protein (GFAP) antigenic sites in the rabbit retina was shown to be sensitive to aldehyde fixation. In chemically unfixed retina, the polyclonal anti-GFAP labeled Müller cells, astrocytes, and unidentified profiles in the outer plexiform la...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90139-l
更新日期:1990-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.04.007
更新日期:2013-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90075-7
更新日期:1984-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1152
更新日期:1994-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.09.015
更新日期:2016-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2017.10.011
更新日期:2018-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(89)90032-8
更新日期:1989-04-01 00:00:00