Lysosomes are associated with microtubules and not with intermediate filaments in cultured fibroblasts.

abstract：:Tentoxin, a natural cyclic tetrapeptide produced by phytopathogenic fungi from the Alternaria species affects the catalytic function of the chloroplast F(1)-ATPase in certain sensitive species of plants. In this study, we show that the uncompetitive inhibitor tentoxin binds to the alphabeta-interface of the chloroplas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Groth G

更新日期：2002-03-19 00:00:00

abstract：:We introduce and analyze a general model of a population evolving over a network of selectively neutral genotypes. We show that the population's limit distribution on the neutral network is solely determined by the network topology and given by the principal eigenvector of the network's adjacency matrix. Moreover, the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van Nimwegen E,Crutchfield JP,Huynen M

更新日期：1999-08-17 00:00:00

abstract：:Significant liquid-structure theory has been used to calculate the transport properties of the alkali metals. Unlike previous calculations, the volume and temperature dependence of the solid-like structure has been taken into consideration. Equations for the potential energy as a function of volume of the alkali metal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hsu CC,Eyring H

更新日期：1972-06-01 00:00:00

abstract：:Intergroup conflict is a persistent feature of many human societies yet little is known about why individuals participate when doing so imposes a mortality risk. To evaluate whether participation in warfare is associated with reproductive benefits, we present data on participation in small-scale livestock raids among ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Glowacki L,Wrangham R

更新日期：2015-01-13 00:00:00

abstract：:Members of the order Nidovirales express their structural protein ORFs from a nested set of 3' subgenomic mRNAs (sg mRNAs), and for most of these ORFs, a single genomic transcription regulatory sequence (TRS) was identified. Nine TRSs were previously reported for the arterivirus Simian hemorrhagic fever virus (SHFV). ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Di H,Madden JC Jr,Morantz EK,Tang HY,Graham RL,Baric RS,Brinton MA

更新日期：2017-10-17 00:00:00

abstract：:Current evidence suggests that the length of poly(A) tails of bacterial mRNAs result from a competition between poly(A) polymerase and exoribonucleases that attack the 3' ends of RNAs. Here, we show that host factor Hfq is also involved in poly(A) tail metabolism. Inactivation of the hfq gene reduces the length of pol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hajnsdorf E,Régnier P

更新日期：2000-02-15 00:00:00

abstract：:Signaling lymphocytic activation molecule family 3 (SLAMF3/Ly9) is a coregulatory molecule implicated in T-cell activation and differentiation. Systemic lupus erythematosus (SLE) is characterized by aberrant T-cell activation and compromised IL-2 production, leading to abnormal regulatory T-cell (Treg) development/fun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Comte D,Karampetsou MP,Kis-Toth K,Yoshida N,Bradley SJ,Mizui M,Kono M,Solomon JR,Kyttaris VC,Tsokos GC

更新日期：2016-08-16 00:00:00

abstract：:We are interested in tracking changes in large-scale data by periodically creating an agglomerative clustering and examining the evolution of clusters (communities) over time. We examine a large real-world data set: the NEC CiteSeer database, a linked network of >250,000 papers. Tracking changes over time requires a c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hopcroft J,Khan O,Kulis B,Selman B

更新日期：2004-04-06 00:00:00

abstract：:Staphylococcus aureus surface protein SasG promotes cell-cell adhesion during the accumulation phase of biofilm formation, but the molecular basis of this interaction remains poorly understood. Here, we unravel the mechanical properties of SasG on the surface of living bacteria, that is, in its native cellular environ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Formosa-Dague C,Speziale P,Foster TJ,Geoghegan JA,Dufrêne YF

更新日期：2016-01-12 00:00:00

abstract：:Bacteria in the human oral cavity often grow in an attached multispecies biofilm community. Members of this community display defined interactions that have an impact on the physiology of the individual and the group. Here, we show that during coculture growth with streptococci, the oral pathogen Aggregatibacter actin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramsey MM,Whiteley M

更新日期：2009-02-03 00:00:00

abstract：:In the presence of N(alpha),N(epsilon)-diacetyl-L-Lys-D-Ala-D-Ala as donor, and either D-[(14)C]alanine, [(14)C]-glycine, or meso-[(3)H]diaminopimelic acid as acceptor, the DD carboxypeptidases from Streptomyces R61 and R39 catalyze a transpeptidation reaction with the release of terminal D-alanine from the donor and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pollock JJ,Ghuysen JM,Linder R,Salton MR,Perkins HR,Nieto M,Leyh-Bouille M,Frere JM,Johnson K

更新日期：1972-03-01 00:00:00

abstract：:From a consideration of (varphi, Psi) values of the amino acids of myoglobin, lysozyme, the alpha and beta chains of horse oxyhemoglobin, tosyl-alpha-chymotrypsin, and carboxypeptidase A, an empirical procedure of predicting whether amino-acid residues in proteins are in a non-helical or may be in a helical conformati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu TT,Kabat EA

更新日期：1971-07-01 00:00:00

abstract：:T cells that have been immunized to express optimal levels of contact hypersensitivity upon adoptive transfer to normal animals can be inhibited from doing so by incubating them with an antigen-specific T suppressor factor. This factor is composed of at least two subunits which come from cells expressing different Ly ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ptak W,Rosenstein RW,Gershon RK

更新日期：1982-04-01 00:00:00

abstract：:Human T lymphocytes were shown to proliferate in response to tetanus toxoid antigen only in the presence of macrophages. This response was inhibited by anti-DRw but not by anti-HLA (A and B loci) antisera added to the cultures and by pretreatment of macrophages but not of T cells with anti-DRw antisera and complement....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Geha RS,Milgrom H,Broff M,Alpert S,Martin S,Yunis EJ

更新日期：1979-08-01 00:00:00

abstract：:Cellular progenitors of the coronary vasculature are believed to enter the chicken heart during epicardial morphogenesis between stages 17 and 27 (days 3-5) of egg incubation. To trace cells which give rise to the coronary arteries in vivo, we applied retroviral cell tagging procedures and analyzed clonal populations ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mikawa T,Fischman DA

更新日期：1992-10-15 00:00:00

abstract：:Maternal microchimerism (MMc) has been associated with development of allospecific transplant tolerance, antitumor immunity, and cross-generational reproductive fitness, but its mode of action is unknown. We found in a murine model that MMc caused exposure to the noninherited maternal antigens in all offspring, but in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bracamonte-Baran W,Florentin J,Zhou Y,Jankowska-Gan E,Haynes WJ,Zhong W,Brennan TV,Dutta P,Claas FH,van Rood JJ,Burlingham WJ

更新日期：2017-01-31 00:00:00

abstract：:Leukemia cell lines of the monocytic series (HL-60, U-937, and P388D1) produce a hepatocyte-stimulating factor (HSF) following induction of differentiation with phorbol diester. In 24-72 hr, these leukemia cells produce 2-30% the amount of HSF as human peripheral blood monocytes. Cells of the series at earlier stages ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Woloski BM,Fuller GM

更新日期：1985-03-01 00:00:00

abstract：:Expression of the bovine papillomavirus E2 regulatory protein in human cervical carcinoma cell lines repressed expression of the resident human papillomavirus E6 and E7 oncogenes and within a few days caused essentially all of the cells to synchronously display numerous phenotypic markers characteristic of cells under...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goodwin EC,Yang E,Lee CJ,Lee HW,DiMaio D,Hwang ES

更新日期：2000-09-26 00:00:00

abstract：:Rod-shaped "ghosts" that are free of murein have been isolated from E. coli. The shape of these "ghosts" is maintained by a unit membrane soluble in sodium dodecyl sulfate. Ghosts consist of about 20-30% phospholipid (almost exclusively phosphatidylethanolamine) and 50-60% protein; a large fraction of the remaining ma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Henning U,Rehn K,Hoehn B

更新日期：1973-07-01 00:00:00

abstract：:Evolution of an oxygenic atmosphere required primordial life to accommodate the toxicity associated with reactive oxygen species. We have characterized an archaeal antioxidant from the hyperthermophilic acidophile Sulfolobus solfataricus. The amino acid sequence of this approximately 22-kDa protein shares little seque...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wiedenheft B,Mosolf J,Willits D,Yeager M,Dryden KA,Young M,Douglas T

更新日期：2005-07-26 00:00:00

abstract：:Humans have altered natural patterns of fire for millennia, but the impact of human-set fires is thought to have been slight in wet closed-canopy forests. In the South Island of New Zealand, Polynesians (Māori), who arrived 700-800 calibrated years (cal y) ago, and then Europeans, who settled ∼150 cal y ago, used fire...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McWethy DB,Whitlock C,Wilmshurst JM,McGlone MS,Fromont M,Li X,Dieffenbacher-Krall A,Hobbs WO,Fritz SC,Cook ER

更新日期：2010-12-14 00:00:00

abstract：:Pheromone biosynthesis-activating neuropeptide (PBAN) regulates sex pheromone biosynthesis in female Helicoverpa (Heliothis) zea. Two oligonucleotide probes representing two overlapping amino acid regions of PBAN were used to screen 2.5 x 10(5) recombinant plaques, and a positive recombinant clone was isolated. Sequen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davis MT,Vakharia VN,Henry J,Kempe TG,Raina AK

更新日期：1992-01-01 00:00:00

abstract：:Molecular cloning has established the primary structures of two precursors of the human pancreas growth hormone-releasing factor (hpGRF-44), somatocrinin. Both polypeptides contain the sequence of hpGRF-44 flanked by basic processing sites. Furthermore, the precursors include a putative signal sequence and a carboxyl-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gubler U,Monahan JJ,Lomedico PT,Bhatt RS,Collier KJ,Hoffman BJ,Böhlen P,Esch F,Ling N,Zeytin F,Brazeau P,Poonian MS,Gage LP

更新日期：1983-07-01 00:00:00

abstract：:Heteroduplex molecules of pneumococcal DNA, prepared by cross-annealing resolved complementary strands, have been used as donors in transformation. A number of pairs of genetic markers are situated exclusively in the trans configuration in these heteroduplexes. Insertion of two markers from trans configuration into op...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roger M

更新日期：1972-02-01 00:00:00

abstract：:Lambdoid phages form clear plaques and show reduced ability to establish immunity in strains of Escherichia coli that lack adenylate cyclase or catabolite activator protein. The absence of the activator protein or cyclic AMP reduces the frequency of lysogenization, but does not prevent steady-state repressor synthesis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grodzicker T,Arditti RR,Eisen H

更新日期：1972-02-01 00:00:00

abstract：:We have analyzed the cell cycle regulation of human cyclin-dependent kinase 2 (CDK2), a protein closely related to the cell cycle-regulatory protein kinase CDC2. We find that CDK2 activity, like that of CDC2, oscillates during the cell cycle in cultured mammalian fibroblasts. Unlike CDC2 activity (which peaks during m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rosenblatt J,Gu Y,Morgan DO

更新日期：1992-04-01 00:00:00

abstract：:The maytansinoid drug DM1 is 100- to 1000-fold more cytotoxic than anticancer drugs that are currently in clinical use. The immunoconjugate C242-DM1 was prepared by conjugating DM1 to the monoclonal antibody C242, which recognizes a mucin-type glycoprotein expressed to various extents by human colorectal cancers. C242...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu C,Tadayoni BM,Bourret LA,Mattocks KM,Derr SM,Widdison WC,Kedersha NL,Ariniello PD,Goldmacher VS,Lambert JM,Blättler WA,Chari RV

更新日期：1996-08-06 00:00:00

abstract：:Natural killer (NK) cells are key players in the innate response to viruses, including herpesviruses. In particular, the variety of viral strategies to modulate the recognition of certain herpesviruses witnesses the importance of NK cells in the control of this group of viruses. Still, NK evasion strategies have remai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grauwet K,Cantoni C,Parodi M,De Maria A,Devriendt B,Pende D,Moretta L,Vitale M,Favoreel HW

更新日期：2014-11-11 00:00:00

abstract：:Folding mechanisms of proteins incorporated within supramolecular assemblies, including viruses, are little understood and may differ fundamentally from folding mechanisms of small globular proteins. We describe a novel Raman dynamic probe of hydrogen-isotope exchange to investigate directly these protein folding/asse...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tuma R,Prevelige PE Jr,Thomas GJ Jr

更新日期：1998-08-18 00:00:00

abstract：:In Trimenia moorei, an extant member of the ancient angiosperm clade Austrobaileyales, we found a remarkable pattern of female gametophyte (egg-producing structure) development that strikingly resembles that of pollen tubes and their intrasexual competition within the maternal pollen tube transmitting tissues of most ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bachelier JB,Friedman WE

更新日期：2011-07-26 00:00:00