Host factor Hfq of Escherichia coli stimulates elongation of poly(A) tails by poly(A) polymerase I.

abstract：:We report here the discovery of a small molecule inhibitor of pestivirus replication. The compound, designated VP32947, inhibits the replication of bovine viral diarrhea virus (BVDV) in cell culture at a 50% inhibitory concentration of approximately 20 nM. VP32947 inhibits both cytopathic and noncytopathic pestiviruse...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baginski SG,Pevear DC,Seipel M,Sun SC,Benetatos CA,Chunduru SK,Rice CM,Collett MS

更新日期：2000-07-05 00:00:00

abstract：:Dense granules of platelets contain a high content of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A). We have previously demonstrated an antithrombotic effect of this compound in a live rabbit model. In the present study we find that certain synthetic Ap4A analogues are superior to Ap4A in inhibiting ADP-induced aggr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zamecnik PC,Kim B,Gao MJ,Taylor G,Blackburn GM

更新日期：1992-03-15 00:00:00

abstract：:The pathogenicity of fixed rabies virus strains for adult mice depends on the presence of an antigenic determinant on the viral glycoprotein. Two virus-neutralizing monoclonal antibodies have been used to identify this determinant. All pathogenic strains of fixed rabies virus bind to these antibodies and are neutraliz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dietzschold B,Wunner WH,Wiktor TJ,Lopes AD,Lafon M,Smith CL,Koprowski H

更新日期：1983-01-01 00:00:00

abstract：:Noncatalytic carbohydrate binding modules (CBMs) are components of glycoside hydrolases that attack generally inaccessible substrates. CBMs mediate a two- to fivefold elevation in the activity of endo-acting enzymes, likely through increasing the concentration of the appended enzymes in the vicinity of the substrate. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cuskin F,Flint JE,Gloster TM,Morland C,Baslé A,Henrissat B,Coutinho PM,Strazzulli A,Solovyova AS,Davies GJ,Gilbert HJ

更新日期：2012-12-18 00:00:00

abstract：:Major changes in the mRNA population of murine liver occur after administration of bacterial lipopolysaccharide, an agent that causes increases in the concentrations of acute-phase serum proteins. The mRNA for one of these, serum amyloid A, is increased at least 500-fold compared to the normal level. It becomes one of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morrow JF,Stearman RS,Peltzman CG,Potter DA

更新日期：1981-08-01 00:00:00

abstract：:The serological properties of the gag gene products p15 and p12 of N- and B-tropic viruses of C57BL mice have been examined. Although these viruses were serologically identical by competition assays for proteins gp71 and p30, they were readily distinguishable in competition assays for proteins p15 and p12. Two isolate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benade LE,Ihle JN,Declève A

更新日期：1978-09-01 00:00:00

abstract：:The cat tongue is covered in sharp, rear-facing spines called papillae, the precise function of which is a mystery. In this combined experimental and theoretical study, we use high-speed film, grooming force measurements, and computed tomography (CT) scanning to elucidate the mechanism by which papillae are used to gr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Noel AC,Hu DL

更新日期：2018-12-04 00:00:00

abstract：:DL-1,2-Dioctanoylglycerol (1,2-DiC8) added to human peripheral resting T lymphocytes was rapidly metabolized to produce octanoic acid and further to small molecules, probably by the action of diacylglycerol lipase and/or nonspecific esterase. Only a small portion was converted to the corresponding phosphatidic acid or...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Asaoka Y,Oka M,Yoshida K,Nishizuka Y

更新日期：1991-10-01 00:00:00

abstract：:Significant attention has focused on the role of low-density lipoprotein (LDL) in the pathogenesis of atherosclerosis. However, recent advances have identified triglyceride-rich lipoproteins [e.g., very LDL (VLDL)] as independent risk predictors for this disease. We have previously demonstrated peroxisome proliferator...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee CH,Kang K,Mehl IR,Nofsinger R,Alaynick WA,Chong LW,Rosenfeld JM,Evans RM

更新日期：2006-02-14 00:00:00

abstract：:Genetic variation in the serotonin transporter gene (SLC6A4) is associated with vulnerability to affective disorders and pharmacotherapy efficacy. We recently identified sequence polymorphisms in the common marmoset SLC6A4 repeat region (AC/C/G and CT/T/C) associated with individual differences in anxiety-like trait, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Santangelo AM,Sawiak SJ,Fryer T,Hong Y,Shiba Y,Clarke HF,Riss PJ,Ferrari V,Tait R,Suckling J,Aigbirhio FI,Roberts AC

更新日期：2019-07-16 00:00:00

abstract：:Elucidation of mechanisms that regulate hematopoietic stem cell self-renewal and differentiation would be facilitated by the identification of defined culture conditions that allow these cells to be amplified. We now demonstrate a significant net increase (3-fold, P < 0.001) in vitro of cells that are individually abl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller CL,Eaves CJ

更新日期：1997-12-09 00:00:00

abstract：:Escherichia coli O157:H7, a toxin-producing food and waterborne bacterial pathogen, has been linked to large outbreaks of gastrointestinal illness for more than two decades. E. coli O157 causes a wide range of clinical illness that varies by outbreak, although factors that contribute to variation in disease severity a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Manning SD,Motiwala AS,Springman AC,Qi W,Lacher DW,Ouellette LM,Mladonicky JM,Somsel P,Rudrik JT,Dietrich SE,Zhang W,Swaminathan B,Alland D,Whittam TS

更新日期：2008-03-25 00:00:00

abstract：:Thioredoxin (Trx) is an intracellular redox protein with extracellular cytokine-like and chemokine-like activities. We show here that, although plasma Trx levels are unrelated to survival of HIV-infected individuals with CD4 cell counts above 200/microl blood, survival is significantly impaired (P = 0.003) when plasma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakamura H,De Rosa SC,Yodoi J,Holmgren A,Ghezzi P,Herzenberg LA,Herzenberg LA

更新日期：2001-02-27 00:00:00

abstract：:The best-studied cytoskeletal system is the inner surface of the erythrocyte membrane, which provides an erythrocyte with the structural support needed to be stable yet flexible as it passes through the circulation. Current structural models predict that the spectrin-actin-based cytoskeletal network is attached to the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stefanovic M,Markham NO,Parry EM,Garrett-Beal LJ,Cline AP,Gallagher PG,Low PS,Bodine DM

更新日期：2007-08-28 00:00:00

abstract：:A unique property of the mammalian embryo is that stem cells can be derived from its early tissue lineages. These lineages will give rise to the fetus as well as essential extraembryonic tissues. Understanding how chromatin regulation participates in establishment of these lineages in the embryo and their derived stem...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rugg-Gunn PJ,Cox BJ,Ralston A,Rossant J

更新日期：2010-06-15 00:00:00

abstract：:In many plants, osmotic stress induces a rapid accumulation of proline through de novo synthesis from glutamate. This response is thought to play a pivotal role in osmotic stress tolerance [Kishor, P. B. K., Hong, Z., Miao, G.-H., Hu, C.-A. A. and Verma, D. P. S. (1995) Plant Physiol. 108, 1387-1394]. During recovery ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Verbruggen N,Hua XJ,May M,Van Montagu M

更新日期：1996-08-06 00:00:00

abstract：:Gastrointestinal infections often induce epithelial damage that must be repaired for optimal gut function. While intestinal stem cells are critical for this regeneration process [R. C. van der Wath, B. S. Gardiner, A. W. Burgess, D. W. Smith, PLoS One 8, e73204 (2013); S. Kozar et al., Cell Stem Cell 13, 626-633 (2013...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mileto SJ,Jardé T,Childress KO,Jensen JL,Rogers AP,Kerr G,Hutton ML,Sheedlo MJ,Bloch SC,Shupe JA,Horvay K,Flores T,Engel R,Wilkins S,McMurrick PJ,Lacy DB,Abud HE,Lyras D

更新日期：2020-04-07 00:00:00

abstract：:Host factor protein Cyclophilin A (CypA) regulates HIV-1 viral infectivity through direct interactions with the viral capsid, by an unknown mechanism. CypA can either promote or inhibit viral infection, depending on host cell type and HIV-1 capsid (CA) protein sequence. We have examined the role of conformational dyna...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu M,Hou G,Zhang H,Suiter CL,Ahn J,Byeon IJ,Perilla JR,Langmead CJ,Hung I,Gor'kov PL,Gan Z,Brey W,Aiken C,Zhang P,Schulten K,Gronenborn AM,Polenova T

更新日期：2015-11-24 00:00:00

abstract：:Genetic studies of the white locus have shown that it has a distal region where structural mutations occur and a proximal region where regulatory mutations occur. To better understand the molecular basis of this genetic organization we have analyzed white locus transcription. A 2.7-kilobase transcript comprising 0.000...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'hare K,Levis R,Rubin GM

更新日期：1983-11-01 00:00:00

abstract：:Magnetic interactions between dissolved oxygen and nitroxide radical spin probes lead to broadening of the ESR lines. We have used a closed-chamber method based on this property to determine the maximum rate of O2 uptake per cell (Vmax per cell) in cultured mammalian cells. A suitable spin probe and a cell suspension ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lai CS,Hopwood LE,Hyde JS,Lukiewicz S

更新日期：1982-02-01 00:00:00

abstract：:The clonal rat pituitary cell line GH4C1 expresses the genes for several voltage-dependent potassium channels including Kv1.5 and Kv1.4. Dexamethasone, a glucocorticoid agonist, induces a slowly inactivating potassium current in these cells but does not alter the amplitude of a rapidly inactivating component of potass...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chung S,Saal DB,Kaczmarek LK

更新日期：1995-06-20 00:00:00

abstract：:Cyclooxygenase-2 (COX-2) expression is induced by mitogenic and proinflammatory factors. Its overexpression plays a causal role in inflammation and tumorigenesis. COX-2 expression is tightly regulated, but the mechanisms are largely unclear. Here we show the control of COX-2 expression by an endogenous tryptophan meta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheng HH,Kuo CC,Yan JL,Chen HL,Lin WC,Wang KH,Tsai KK,Guvén H,Flaberg E,Szekely L,Klein G,Wu KK

更新日期：2012-08-14 00:00:00

abstract：:Wild barley (Hordeum spontaneum) represents a significant genetic resource for crop improvement in barley (Hordeum vulgare) and for the study of the evolution and domestication of plant populations. The Isa gene from barley has a putative role in plant defense. This gene encodes a bifunctional alpha-amylase/subtilisin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cronin JK,Bundock PC,Henry RJ,Nevo E

更新日期：2007-02-20 00:00:00

abstract：:CD83 is commonly known as a specific marker for mature dendritic cells. It has been shown to be important for CD4(+) T-cell development in the thymus. However, its function in the peripheral immune system remains enigmatic. Here, we show that CD83 inhibits proliferation and production of IL-2 and IFN-γ by T cells, and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen L,Zhu Y,Zhang G,Gao C,Zhong W,Zhang X

更新日期：2011-11-15 00:00:00

abstract：:The key enzyme for transcription of protein-encoding genes in eukaryotes is RNA polymerase II (RNAPII). The recruitment of this enzyme during transcription initiation and its passage along the template during transcription elongation is regulated through the association and dissociation of several complexes. Elongator...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nelissen H,Fleury D,Bruno L,Robles P,De Veylder L,Traas J,Micol JL,Van Montagu M,Inzé D,Van Lijsebettens M

更新日期：2005-05-24 00:00:00

abstract：:The developmental plasticity of plants relies on the remarkable ability of the meristems to integrate nutrient and energy availability with environmental signals. Meristems in root and shoot apexes share highly similar molecular players but are spatially separated by soil. Whether and how these two meristematic tissue...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li X,Cai W,Liu Y,Li H,Fu L,Liu Z,Xu L,Liu H,Xu T,Xiong Y

更新日期：2017-03-07 00:00:00

abstract：:By using differential display PCR, we have identified 98 cDNA fragments from the rat dorsal hippocampus that are expressed differentially between the fast learners and slow learners in the water maze learning task. One of these cDNA fragments encodes the rat serum- and glucocorticoid-inducible kinase (sgk) gene. North...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsai KJ,Chen SK,Ma YL,Hsu WL,Lee EH

更新日期：2002-03-19 00:00:00

abstract：:During latent infection of humans with Mycobacterium tuberculosis, bacteria persist in the asymptomatic host within granulomas, organized collections of differentiated macrophages, and other immune cells. The mechanisms for persistence remain poorly understood, as is the metabolic and replicative state of the microbes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chan K,Knaak T,Satkamp L,Humbert O,Falkow S,Ramakrishnan L

更新日期：2002-03-19 00:00:00

abstract：:Cities are concentrated areas of CO2 emissions and have become the foci of policies for mitigation actions. However, atmospheric measurement networks suitable for evaluating urban emissions over time are scarce. Here we present a unique long-term (decadal) record of CO2 mole fractions from five sites across Utah's met...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitchell LE,Lin JC,Bowling DR,Pataki DE,Strong C,Schauer AJ,Bares R,Bush SE,Stephens BB,Mendoza D,Mallia D,Holland L,Gurney KR,Ehleringer JR

更新日期：2018-03-20 00:00:00

abstract：:Protein-protein interactions are essential for life. Yet, our understanding of the general principles governing binding is not complete. In the present study, we show that the interface between proteins is built in a modular fashion; each module is comprised of a number of closely interacting residues, with few intera...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reichmann D,Rahat O,Albeck S,Meged R,Dym O,Schreiber G

更新日期：2005-01-04 00:00:00