Analogues of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) as potential anti-platelet-aggregation agents.

abstract：:A remote labeling method has been developed to determine (18)O kinetic isotope effects (KIEs) in Ras-catalyzed GTP hydrolysis. Substrate mixtures consist of (13)C-depleted GTP and [(18)O,(13)C]GTP that contains (18)O at phosphoryl positions of mechanistic interest and (13)C at all carbon positions of the guanosine moi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Du X,Black GE,Lecchi P,Abramson FP,Sprang SR

更新日期：2004-06-15 00:00:00

abstract：:The ability of the isolated perfused rat liver to differentiate between chylomicrons and remnants with either high or low apoprotein E:C ratios was investigated. Remnants were prepared in hepatectomized rats injected with chylomicrons double-labeled with [3H]cholesterol and 14C-labeled fatty acids. By densitometric sc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Borensztajn J,Kotlar TJ

更新日期：1984-09-01 00:00:00

abstract：:Control of iron homeostasis is essential for healthy central nervous system function: iron deficiency is associated with cognitive impairment, yet iron overload is thought to promote neurodegenerative diseases. Specific genetic markers have been previously identified that influence levels of transferrin, the protein t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jahanshad N,Kohannim O,Hibar DP,Stein JL,McMahon KL,de Zubicaray GI,Medland SE,Montgomery GW,Whitfield JB,Martin NG,Wright MJ,Toga AW,Thompson PM

更新日期：2012-04-03 00:00:00

abstract：:The Escherichia coli mutS gene product is involved in mismatch correction in this organism. We have purified a biologically active form of the 97,000 Mr protein to near homogeneity from an overproducing strain. Enzymatic and chemical protection ("footprinting") experiments have demonstrated that mutS-encoded protein s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Su SS,Modrich P

更新日期：1986-07-01 00:00:00

abstract：:Most guanine nucleotide binding protein (G protein)-coupled receptors have a conserved cysteine in the C-terminal cytoplasmic tail near the seventh transmembrane spanning region. This cysteine is known to be palmitoylated in rhodopsin, the beta 2-adrenergic receptor (beta 2AR) and the alpha 2A-adrenergic receptor (alp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eason MG,Jacinto MT,Theiss CT,Liggett SB

更新日期：1994-11-08 00:00:00

abstract：:The DNA sequence of a tryptophan synthase gene and the flanking 5' and 3' regions has been determined for Arabidopsis thaliana. The sequence encodes only the beta subunit domain, indicating that alpha and beta subunits are specified by separate genes. The gene contains four introns and encodes 470 amino acid residues....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berlyn MB,Last RL,Fink GR

更新日期：1989-06-01 00:00:00

abstract：:Usher syndrome type 1 (USH1) patients suffer from sensorineuronal deafness, vestibular dysfunction, and visual impairment. Several genetic loci have been linked to USH1, and four of the relevant genes have been identified. They encode the unconventional myosin VIIa, the PDZ-domain protein harmonin, and the putative ad...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Siemens J,Kazmierczak P,Reynolds A,Sticker M,Littlewood-Evans A,Müller U

更新日期：2002-11-12 00:00:00

abstract：:Human languages show a remarkable degree of variation in the area they cover. However, the factors governing the distribution of human cultural groups such as languages are not well understood. While previous studies have examined the role of a number of environmental variables the importance of cultural factors has n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Currie TE,Mace R

更新日期：2009-05-05 00:00:00

abstract：:Amplitude-modulated phosphorescence/microwave double-resonance (AM-PMDR) spectra are reported for complexes of methylmercury(II) cation, designated CH3Hg(II), with tryptophan and glyceraldehyde-3-phosphate dehydrogenase (GPDHase; from rabbit muscle). Wavelength shifts are observed in the AM-PMDR spectra of CH3Hg(II)-t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davis JM,Maki AH

更新日期：1982-07-01 00:00:00

abstract：:Repetitive sequences are hotspots of evolution at multiple levels. However, due to difficulties involved in their assembly and analysis, the role of repeats in tumor evolution is poorly understood. We developed a rigorous motif-based methodology to quantify variations in the repeat content, beyond microsatellites, in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Persi E,Prandi D,Wolf YI,Pozniak Y,Barnabas GD,Levanon K,Barshack I,Barbieri C,Gasperini P,Beltran H,Faltas BM,Rubin MA,Geiger T,Koonin EV,Demichelis F,Horn D

更新日期：2019-08-20 00:00:00

abstract：:Recent studies have shown that the genes of the gibberellin (GA) biosynthesis pathway in the fungus Gibberella fujikuroi are organized in a cluster of at least seven genes. P450-1 is one of four cytochrome P450 monooxygenase genes in this cluster. Disruption of the P450-1 gene in the GA-producing wild-type strain IMI ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rojas MC,Hedden P,Gaskin P,Tudzynski B

更新日期：2001-05-08 00:00:00

abstract：:Gene function is typically evaluated by sampling the continuum of gene expression at only a few discrete points corresponding to gene knockout or overexpression. We argue that this characterization is incomplete and present a library of engineered promoters of varying strengths obtained through mutagenesis of a consti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alper H,Fischer C,Nevoigt E,Stephanopoulos G

更新日期：2005-09-06 00:00:00

abstract：:Tibetans have adapted to the chronic hypoxia of high altitude and display a distinctive suite of physiologic adaptations, including augmented hypoxic ventilatory response and resistance to pulmonary hypertension. Genome-wide studies have consistently identified compelling genetic signatures of natural selection in two...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Song D,Navalsky BE,Guan W,Ingersoll C,Wang T,Loro E,Eeles L,Matchett KB,Percy MJ,Walsby-Tickle J,McCullagh JSO,Medina RJ,Khurana TS,Bigham AW,Lappin TR,Lee FS

更新日期：2020-06-02 00:00:00

abstract：:The cerebral cortex of mammals differentiates into functionally distinct areas that exhibit unique cytoarchitecture, connectivity, and molecular characteristics. Molecular specification of cells fated for limbic cortical areas, based on the expression of the limbic system-associated membrane protein (LAMP), occurs dur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barbe MF,Levitt P

更新日期：1992-05-01 00:00:00

abstract：:Wild-type p53 protein is a growth modulator whose inactivation has been found to be a key event in malignant transformation. Reconstitution of wild-type p53 in the p53-nonproducer, Abelson murine leukemia virus-transformed pre-B-cell line L12 gave rise to stably growing clones. Wild-type p53-producer derived cell line...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shaulsky G,Goldfinger N,Peled A,Rotter V

更新日期：1991-10-15 00:00:00

abstract：:The effect of phagocytosis on lectin binding to plasma membranes of polymorphonuclear leukocytes was examined. The specific activities of binding sites of concanavalin A and Ricinus communis agglutinin (defined as mug of lectin bound per 100 mug of membrane protein) were measured on isolated membranes; they decreased ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oliver JM,Ukena TE,Berlin RD

更新日期：1974-02-01 00:00:00

abstract：:Carbachol, an activator of muscarinic acetylcholine receptors of NG108-15 hybrid cells, inhibits adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] rapidly and reversibly and slowly evokes a 200-300% increase in adenylate cyclase activity over a period of 24-30 hr. Both the inhibition of adenylate cyc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nathanson NM,Klein WL,Nirenberg M

更新日期：1978-04-01 00:00:00

abstract：:We characterize a sonic hedgehog (Shh) signaling domain restricted to the adventitial layer of artery wall that supports resident Sca1-positive vascular progenitor cells (AdvSca1). Using patched-1 (Ptc1(lacZ)) and patched-2 (Ptc2(lacZ)) reporter mice, adventitial Shh signaling activity was first detected at embryonic ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Passman JN,Dong XR,Wu SP,Maguire CT,Hogan KA,Bautch VL,Majesky MW

更新日期：2008-07-08 00:00:00

abstract：:DNA "breathing" is a thermally driven process in which base-paired DNA sequences transiently adopt local conformations that depart from their most stable structures. Polymerases and other proteins of genome expression require access to single-stranded DNA coding templates located in the double-stranded DNA "interior,"...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Phelps C,Lee W,Jose D,von Hippel PH,Marcus AH

更新日期：2013-10-22 00:00:00

abstract：:In movies or on TV, a wheel can seem to rotate backwards, due to the temporal subsampling inherent in the recording process (the wagon wheel illusion). Surprisingly, this effect has also been reported under continuous light, suggesting that our visual system, too, might sample motion in discrete "snapshots." Recently,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： VanRullen R,Reddy L,Koch C

更新日期：2005-04-05 00:00:00

abstract：:Spectrin (betaISigma*) and ankyrin (AnkG119) associate with Golgi membranes and the dynactin complex, but their role in vesicle trafficking remains uncertain. We find that the actin-binding domain and membrane-association domain 1 (MAD1) of betaI spectrin together form a constitutive Golgi targeting signal in transfec...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Devarajan P,Stabach PR,De Matteis MA,Morrow JS

更新日期：1997-09-30 00:00:00

abstract：:Plasmid pT181 is a 4437-base-pair, multicopy plasmid of Staphylococcus aureus that encodes tetracycline resistance. The replication of the leading strand of pT181 DNA initiates by covalent extension of a site-specific nick generated by the initiator protein at the origin of replication and proceeds by an asymmetric ro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Birch P,Khan SA

更新日期：1992-01-01 00:00:00

abstract：:Spiperone, a dopamine antagonist widely used as a specific ligand for dopamine and serotonin receptors, is actively accumulated into the F4C1 strain of rat pituitary tumor cells. The accumulation of 10 nM [3H]spiperone was linear for 3 min and reached a steady state after 10 min. Spiperone accumulation was reduced 50%...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dannies PS,Rudnick MS,Fishkes H,Rudnick G

更新日期：1984-03-01 00:00:00

abstract：:A common genetic mutation found in clear cell renal cell carcinoma (CC-RCC) is the loss of the von Hippel-Lindau (VHL) gene, which results in stabilization of hypoxia-inducible factors (HIFs), and contributes to cancer progression and metastasis. CUB-domain-containing protein 1 (CDCP1) was shown to promote metastasis ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Razorenova OV,Finger EC,Colavitti R,Chernikova SB,Boiko AD,Chan CK,Krieg A,Bedogni B,LaGory E,Weissman IL,Broome-Powell M,Giaccia AJ

更新日期：2011-02-01 00:00:00

abstract：:Gyrate atrophy of the choroid and retina (GA) is an inherited chorioretinal degeneration caused by deficiency of ornithine delta-aminotransferase (OAT; L-ornithine: 2-oxo-acid aminotransferase; EC 2.6.1.13). GA is one of the "Finnish genetic diseases," a group of several rare monogenic disorders that occur with increa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitchell GA,Brody LC,Sipila I,Looney JE,Wong C,Engelhardt JF,Patel AS,Steel G,Obie C,Kaiser-Kupfer M

更新日期：1989-01-01 00:00:00

abstract：:It is not yet clear how bacterial outer membrane proteins reach their correct destination after they are secreted across the cytoplasmic membrane. We show here that porin OmpF is secreted into the medium as a water-soluble monomeric protein by spheroplasts of Escherichia coli. Furthermore, this monomeric porin is take...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sen K,Nikaido H

更新日期：1990-01-01 00:00:00

abstract：:Pin1 is a modular enzyme that accelerates the cis-trans isomerization of phosphorylated-Ser/Thr-Pro (pS/T-P) motifs found in numerous signaling proteins regulating cell growth and neuronal survival. We have used NMR to investigate the interaction of Pin1 with three related ligands that include a pS-P substrate peptide...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Namanja AT,Wang XJ,Xu B,Mercedes-Camacho AY,Wilson KA,Etzkorn FA,Peng JW

更新日期：2011-07-26 00:00:00

abstract：:The 3' flanking region of the apolipoprotein B (apoB) gene contains a hypervariable region consisting of a variable number of tandemly repeated short A + T-rich DNA sequences (VNTRs). We present a general method that utilizes the polymerase chain reaction to rapidly and accurately type this and other VNTR loci. We use...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boerwinkle E,Xiong WJ,Fourest E,Chan L

更新日期：1989-01-01 00:00:00

abstract：:The evolution of insect resistance to pesticides poses a continuing threat to agriculture and human health. While much is known about the proximate molecular and biochemical mechanisms that confer resistance, far less is known about the regulation of the specific genes/gene families involved, particularly by trans-act...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang X,Deng S,Wei X,Yang J,Zhao Q,Yin C,Du T,Guo Z,Xia J,Yang Z,Xie W,Wang S,Wu Q,Yang F,Zhou X,Nauen R,Bass C,Zhang Y

更新日期：2020-05-12 00:00:00

abstract：:The transcription factor and tumor suppressor protein p53 is frequently inactivated in human cancers. In many cases, p53 gene mutations result in high levels of inactive, full-length p53 protein with one amino acid change in the core domain that recognizes p53 DNA-binding sites. The ability to endow function to mutate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baroni TE,Wang T,Qian H,Dearth LR,Truong LN,Zeng J,Denes AE,Chen SW,Brachmann RK

更新日期：2004-04-06 00:00:00