Abstract:
:Carbachol, an activator of muscarinic acetylcholine receptors of NG108-15 hybrid cells, inhibits adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] rapidly and reversibly and slowly evokes a 200-300% increase in adenylate cyclase activity over a period of 24-30 hr. Both the inhibition of adenylate cyclase and the gradual increase in enzyme activity are dependent on muscarinic acetylcholine receptors and the receptor activator. Withdrawal of carbachol results in a gradual return of adenylate cyclase activity to control levels over a period of 6 hr; the half-life for decay of enzyme activity is 1.6 hr. These results show that muscarinic acetylcholine receptors mediate both transient and long-lived effects on adenylate cyclase activity that resemble those of opiates.
journal_name
Proc Natl Acad Sci U S Aauthors
Nathanson NM,Klein WL,Nirenberg Mdoi
10.1073/pnas.75.4.1788subject
Has Abstractpub_date
1978-04-01 00:00:00pages
1788-91issue
4eissn
0027-8424issn
1091-6490journal_volume
75pub_type
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