Metabolic rate of membrane-permeant diacylglycerol and its relation to human resting T-lymphocyte activation.

abstract：:The synthesis of cytoplasmic proteins from quiescent and serum-stimulated Swiss 3T3 cells was compared by two-dimensional polyacrylamide gel electrophoresis. Four new proteins of Mrs 26,000, 28,000, 45,000, and 47,000 designated N26, N28, N45, and N47, which were not detectable in quiescent cells, appeared 60 min afte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thomas G,Thomas G,Luther H

更新日期：1981-09-01 00:00:00

abstract：:Phosphatidylcholine (PC) is the major glycerophospholipid in eukaryotic cells and is an essential component in all cellular membranes. The biochemistry of de novo PC synthesis by the Kennedy pathway is well established, but less is known about the physiological functions of PC. We identified two unrelated patients wit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Payne F,Lim K,Girousse A,Brown RJ,Kory N,Robbins A,Xue Y,Sleigh A,Cochran E,Adams C,Dev Borman A,Russel-Jones D,Gorden P,Semple RK,Saudek V,O'Rahilly S,Walther TC,Barroso I,Savage DB

更新日期：2014-06-17 00:00:00

abstract：:Nociceptive information is modulated by a large number of endogenous signaling agents that change over the course of recovery from injury. This plasticity makes understanding regulatory mechanisms involved in descending inhibition of pain scientifically and clinically important. Neurons that synthesize the neuropeptid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dimitrov EL,Kuo J,Kohno K,Usdin TB

更新日期：2013-08-06 00:00:00

abstract：:Multiple independent mutations in neuroligin genes were identified in patients with familial autism, including the R451C substitution in neuroligin-3 (NL3). Previous studies showed that NL3(R451C) knock-in mice exhibited modestly impaired social behaviors, enhanced water maze learning abilities, and increased synaptic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Etherton M,Földy C,Sharma M,Tabuchi K,Liu X,Shamloo M,Malenka RC,Südhof TC

更新日期：2011-08-16 00:00:00

abstract：:Impaired wound closure is a growing medical problem associated with metabolic diseases and aging. Immune cells play important roles in wound healing by following instructions from the microenvironment. Here, we developed a technology to bioengineer the wound microenvironment and enhance healing abilities of the immune...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vågesjö E,Öhnstedt E,Mortier A,Lofton H,Huss F,Proost P,Roos S,Phillipson M

更新日期：2018-02-20 00:00:00

abstract：:In savannas, predicting how vegetation varies is a longstanding challenge. Spatial patterning in vegetation may structure that variability, mediated by spatial interactions, including competition and facilitation. Here, we use unique high-resolution, spatially extensive data of tree distributions in an African savanna...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Staver AC,Asner GP,Rodriguez-Iturbe I,Levin SA,Smit IPJ

更新日期：2019-05-28 00:00:00

abstract：:The lateral mobility of individual murine polyoma virus-like particles (VLPs) bound to live cells and artificial lipid bilayers was studied by single fluorescent particle tracking using total internal reflection fluorescence microscopy. The particle trajectories were analyzed in terms of diffusion rates and modes of m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ewers H,Smith AE,Sbalzarini IF,Lilie H,Koumoutsakos P,Helenius A

更新日期：2005-10-18 00:00:00

abstract：:On the basis of task-related imaging studies in normal human subjects, it has been suggested that two attention systems exist in the human brain: a bilateral dorsal attention system involved in top-down orienting of attention and a right-lateralized ventral attention system involved in reorienting attention in respons...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fox MD,Corbetta M,Snyder AZ,Vincent JL,Raichle ME

更新日期：2006-06-27 00:00:00

abstract：:Endothelium-derived relaxing factor (EDRF) activity has been attributed to the highly labile nitric oxide radical (NO). In view of the fact that the plasma and cellular milieux contain reactive species that can rapidly inactivate NO, it has been postulated that NO is stabilized by a carrier molecule that preserves its...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stamler JS,Simon DI,Osborne JA,Mullins ME,Jaraki O,Michel T,Singel DJ,Loscalzo J

更新日期：1992-01-01 00:00:00

abstract：:Geckos can run rapidly on walls and ceilings, requiring high friction forces (on walls) and adhesion forces (on ceilings), with typical step intervals of approximately 20 ms. The rapid switching between gecko foot attachment and detachment is analyzed theoretically based on a tape model that incorporates the adhesion ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tian Y,Pesika N,Zeng H,Rosenberg K,Zhao B,McGuiggan P,Autumn K,Israelachvili J

更新日期：2006-12-19 00:00:00

abstract：:Neurofibromin gene (NF1) mutation causes neurofibromatosis type 1 (NF1), a disorder in which brain white matter deficits identified by neuroimaging are common, yet of unknown cellular etiology. In mice, Nf1 loss in adult oligodendrocytes causes myelin decompaction and increases oligodendrocyte nitric oxide (NO) levels...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Asleh J,Shofty B,Cohen N,Kavushansky A,López-Juárez A,Constantini S,Ratner N,Kahn I

更新日期：2020-09-08 00:00:00

abstract：:Analyses of amino-acid sequences of the total cell-free products programmed by the mRNA of MOPC-104E gamma light (L)-chain show that over 95% of the products have sequences of a distinct protein that correspond to the L-chain precursor. In this precursor an extra piece is coupled to the NH2-terminus of the mature L-ch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burstein Y,Kantour F,Schechter I

更新日期：1976-08-01 00:00:00

abstract：:Tetrodotoxin was infused into the suprachiasmatic nuclei of unanesthetized and unrestrained rats continuously for 14 days. The internal timekeeping mechanism of the circadian pacemaker in the nuclei continued to oscillate unaffected by this treatment, although the toxin reversibly blocked function of both the input pa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schwartz WJ,Gross RA,Morton MT

更新日期：1987-03-01 00:00:00

abstract：:In virtually all multicellular eukaryotes, mitochondria are transmitted exclusively through one parent, usually the mother. In this short review, we discuss some of the major consequences of uniparental transmission of mitochondria, including deleterious effects in males and selection for increased transmission throug...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Perlman SJ,Hodson CN,Hamilton PT,Opit GP,Gowen BE

更新日期：2015-08-18 00:00:00

abstract：:The frequency of recombination between the G gamma-globin (HBG2) and parathyroid hormone (PTH) loci on the short arm of human chromosome 11 was estimated by typing greater than 700 single-sperm samples from two males. The sperm-typing technique employed involves the polymerase chain reaction and allele-specific oligon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cui XF,Li HH,Goradia TM,Lange K,Kazazian HH Jr,Galas D,Arnheim N

更新日期：1989-12-01 00:00:00

abstract：:High-resolution mapping of quantitative trait loci (QTL) in animals has proved to be difficult because the large effect sizes detected in crosses between inbred strains are often caused by numerous linked QTLs, each of small effect. In a study of fearfulness in mice, we have shown it is possible to fine map small-effe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mott R,Talbot CJ,Turri MG,Collins AC,Flint J

更新日期：2000-11-07 00:00:00

abstract：:Interest in cell-associated collagens led others to isolate A and B collagen chains, also known as type V collagen, from many tissues, but only after pepsin cleavage. Soluble precursors of these chains are synthesized in vitro by crop, a chicken embryo muscle tissue, and are converted by at least two processing steps ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kumamoto CA,Fessler JH

更新日期：1980-11-01 00:00:00

abstract：:To complete the set of synthetic analogs of the three recognized types of active sites in iron-sulfur redox proteins, the compound (Et4N)[Fe((SCH2)2C6H4)2], derived from o-xylyl-alpha,alpha'-dithiol, has been prepared and its structure has been determined by x-ray diffraction. The bischelate anion contains a near-tetr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lane RW,Ibers JA,Frankel RB,Holm RH

更新日期：1975-08-01 00:00:00

abstract：:A technique is described for isolation of plasmid DNA in closed and open circular double-stranded forms from bacterial cells, by use of ATP-dependent deoxyribonuclease purified from Micrococcus luteus. This DNase, acting only upon linear DNA molecules, degrades all bacterial chromosomal DNA extracted in the linear for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mukai T,Matsubara K,Takagi Y

更新日期：1973-10-01 00:00:00

abstract：:Receptor tyrosine kinases (RTKs) control a multitude of biological processes and are therefore subjected to multiple levels of regulation. Negative feedback is one of the mechanisms that provide an effective means to control RTK-mediated signaling. Sef has recently been identified as a specific antagonist of fibroblas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Preger E,Ziv I,Shabtay A,Sher I,Tsang M,Dawid IB,Altuvia Y,Ron D

更新日期：2004-02-03 00:00:00

abstract：:Renal excretion of water and major electrolytes exhibits a significant circadian rhythm. This functional periodicity is believed to result, at least in part, from circadian changes in secretion/reabsorption capacities of the distal nephron and collecting ducts. Here, we studied the molecular mechanisms underlying circ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zuber AM,Centeno G,Pradervand S,Nikolaeva S,Maquelin L,Cardinaux L,Bonny O,Firsov D

更新日期：2009-09-22 00:00:00

abstract：:Song syntax, defined as orderly temporal arrangements of acoustic units within a bird song, is a conspicuous feature of the songs of many species of passerine birds. While syntactical features play a role in interspecific song recognition by males of many bird species, syntax variation within species and female respon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balaban E

更新日期：1988-05-01 00:00:00

abstract：:Overlapping cDNAs derived from the chicken alpha-fibrinogen mRNA have been sequenced, beginning from within the coding region for the signal peptide of this subunit and terminating within the poly(A) extension. The predicted size of chicken alpha-fibrinogen is 54,187 daltons, which is the smallest of any alpha chain r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weissbach L,Grieninger G

更新日期：1990-07-01 00:00:00

abstract：:Ferritin has a protein shell of 5 X 10(6) Da consisting of 24 subunits of two types, a heavier (H) chain of 21,000 Da and a lighter (L) chain of 19,000 Da. A cDNA clone of the messenger for the L subunit has been isolated from a human monocyte-like leukemia cell line. The clone contains an open reading frame of 522 nu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dörner MH,Salfeld J,Will H,Leibold EA,Vass JK,Munro HN

更新日期：1985-05-01 00:00:00

abstract：:Liver ischemia and reperfusion injury (IRI) is a major challenge in liver surgery. Diet restriction reduces liver damage by increasing stress resistance; however, the underlying molecular mechanisms remain unclear. We investigated the preventive effect of 12-h fasting on mouse liver IRI. Partial warm hepatic IRI model...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miyauchi T,Uchida Y,Kadono K,Hirao H,Kawasoe J,Watanabe T,Ueda S,Okajima H,Terajima H,Uemoto S

更新日期：2019-07-02 00:00:00

abstract：:Satellite tobacco necrosis virus (STNV) is one of the smallest viruses known. Its genome encodes only its coat protein (CP) subunit, relying on the polymerase of its helper virus TNV for replication. The genome has been shown to contain a cryptic set of dispersed assembly signals in the form of stem-loops that each pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patel N,Wroblewski E,Leonov G,Phillips SEV,Tuma R,Twarock R,Stockley PG

更新日期：2017-11-14 00:00:00

abstract：:In HL-60 cells, a human promyelocytic leukemia cell line, the human c-myc gene, designated MYC, is amplified about 16-fold. On differentiation of the HL-60 cells into granulocytes induced by several inhibitors of poly(ADP-ribose) polymerase [NAD+ poly(adenosine diphosphate D-ribose)ADP-D-ribosyltransferase, EC 2.4.2.3...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shima H,Nakayasu M,Aonuma S,Sugimura T,Nagao M

更新日期：1989-10-01 00:00:00

abstract：:Under various conditions, specific genetic areas of the phage T4 DNA molecule are preferentially and repeatedly replicated, resulting in the amplification of these areas. These areas are found to lie in the vicinity of the known origins of DNA replication. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kozinski AW,Ling SK,Hutchinson N,Halpern ME,Mattson T

更新日期：1980-09-01 00:00:00

abstract：:A combined genetic, biochemical, and immunological approach has clarified structural relationships involving the first three enzymes of de novo pyrimidine biosynthesis. A procedure involving antibody and protein A-Sepharose was used to isolate the enzymes carbamoyl-phosphate synthase [ATP:carbamate phosphotransferase ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davidson JN,Patterson D

更新日期：1979-04-01 00:00:00

abstract：:We have isolated a human gastrin gene from a genomic library by employing a human gastrin cDNA clone as a hybridization probe. The total length of the gene is approximately 4.0 kilobase pairs, and the gene is separated into three exons and two introns. A 130-base-pair intron interrupts the coding region and a 3.0-kilo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ito R,Sato K,Helmer T,Jay G,Agarwal K

更新日期：1984-08-01 00:00:00