Synthetic analogs of active sites of iron-sulfur proteins: bis (o-xylyldithiolato) ferrate (III) monoanion, a structurally unconstrained model for the rubredoxin Fe-S4 unit.

abstract：:Dysregulation of cyclin-dependent kinase 5 (cdk5) per relative concentrations of its activators p35 and p25 is implicated in neurodegenerative diseases. P35 has a short t½ and undergoes rapid proteasomal degradation in its membrane-bound myristoylated form. P35 is converted by calpain to p25, which, along with an exte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsai SY,Pokrass MJ,Klauer NR,Nohara H,Su TP

更新日期：2015-05-26 00:00:00

abstract：:Cytoplasmic RNA extracted from human tissue culture cells infected with adenovirus type 2 was used to program protein synthesis in a cell-free system derived from mammalian cells. Analysis of the protein product by polyacrylamide gel electrophoresis revealed ten adenovirus-specific polypeptides. Five of these were fur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderson CW,Lewis JB,Atkins JF,Gesteland RF

更新日期：1974-07-01 00:00:00

abstract：:Block of Ca2+ currents by the dihydropyridine drug nitrendipine was studied in single canine ventricular cells by using the whole-cell variant of the patch clamp technique. When cells were held at depolarized membrane potentials at which Ca2+ currents were approximately equal to 70% inactivated, nitrendipine blocked C...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bean BP

更新日期：1984-10-01 00:00:00

abstract：:Proactive policing, the strategic targeting of people or places to prevent crimes, is a well-studied tactic that is ubiquitous in modern law enforcement. A 2017 National Academies of Sciences report reviewed existing literature, entrenched in deterrence theory, and found evidence that proactive policing strategies can...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Del Toro J,Lloyd T,Buchanan KS,Robins SJ,Bencharit LZ,Smiedt MG,Reddy KS,Pouget ER,Kerrison EM,Goff PA

更新日期：2019-04-23 00:00:00

abstract：:The hetR gene plays a very important role in cell differentiation of heterocystous cyanobacteria. To understand the mechanism of the hetR gene product in regulation of heterocyst differentiation, the recombinant HetR protein (rHetR) was overproduced in Escherichia coli. Purified rHetR was unstable and degraded easily ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou R,Wei X,Jiang N,Li H,Dong Y,Hsi KL,Zhao J

更新日期：1998-04-28 00:00:00

abstract：:Protein-mediated electron transfer is an essential event in many biochemical processes. Efficient electron transfer requires the reorganization energy of the redox event to be minimized, which is ensured by the presence of rigid donor and acceptor sites. Electron transfer copper sites are present in the ubiquitous cup...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zaballa ME,Abriata LA,Donaire A,Vila AJ

更新日期：2012-06-12 00:00:00

abstract：:Apolipoprotein L-1 (APOL1), the trypanolytic factor of human serum, can lyse several African trypanosome species including Trypanosoma brucei brucei, but not the human-infective pathogens T. brucei rhodesiense and T. brucei gambiense, which are resistant to lysis by human serum. Lysis follows the uptake of APOL1 into ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thomson R,Finkelstein A

更新日期：2015-03-03 00:00:00

abstract：:Current issues pertaining to theoretical simulations of materials, with a focus on systems of nanometer-scale dimensions, are discussed. The use of atomistic simulations as high-resolution numerical experiments, enabling and guiding formulation and testing of analytic theoretical descriptions, is demonstrated through ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Landman U

更新日期：2005-05-10 00:00:00

abstract：:This paper provides transcendental and algebraic framework for the classification of identities expressing pi and other logarithms of algebraic numbers as rapidly convergent generalized hypergeometric series in rational parameters. Algebraic and arithmetic relations between values of p+1Fp hypergeometric functions and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chudnovsky DV,Chudnovsky GV

更新日期：1998-03-17 00:00:00

abstract：:In neurotransmission synaptotagmin-1 tethers synaptic vesicles to the presynaptic plasma membrane by binding to acidic membrane lipids and SNAREs and promotes rapid SNARE-mediated fusion upon Ca(2+) triggering. However, recent studies suggested that upon membrane contact synaptotagmin may not only bind in trans to the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vennekate W,Schröder S,Lin CC,van den Bogaart G,Grunwald M,Jahn R,Walla PJ

更新日期：2012-07-03 00:00:00

abstract：:The alternative splicing of mRNA is a critical process in higher eukaryotes that generates substantial proteomic diversity. Many of the proteins that are essential to this process contain arginine/serine-rich (RS) domains. ZRANB2 is a widely-expressed and highly-conserved RS-domain protein that can regulate alternativ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Loughlin FE,Mansfield RE,Vaz PM,McGrath AP,Setiyaputra S,Gamsjaeger R,Chen ES,Morris BJ,Guss JM,Mackay JP

更新日期：2009-04-07 00:00:00

abstract：:The poly(A) site of bovine prolactin (bPRL) mRNA was examined by phased priming of cDNA synthesis with oligodeoxynucleotides of the general sequence d(pT8-N-N'). The existence of multiple poly(A)-adjacent sequences in bPRL mRNA was indicated by the production of specific chain-termination fragments with at least three...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sasavage NL,Smith M,Gillam S,Woychik RP,Rottman FM

更新日期：1982-01-01 00:00:00

abstract：:Although de novo protein design is an important endeavor with implications for understanding protein folding, until now, structures have been determined for only a few 25- to 30-residue designed miniproteins. Here, the NMR solution structure of a complex 73-residue three-helix bundle protein, alpha3D, is reported. The...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walsh ST,Cheng H,Bryson JW,Roder H,DeGrado WF

更新日期：1999-05-11 00:00:00

abstract：:Leptin is a pivotal regulator of energy and glucose homeostasis, and defects in leptin signaling result in obesity and diabetes. The ATP-sensitive potassium (K(ATP)) channels couple glucose metabolism to insulin secretion in pancreatic β-cells. In this study, we provide evidence that leptin modulates pancreatic β-cell...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park SH,Ryu SY,Yu WJ,Han YE,Ji YS,Oh K,Sohn JW,Lim A,Jeon JP,Lee H,Lee KH,Lee SH,Berggren PO,Jeon JH,Ho WK

更新日期：2013-07-30 00:00:00

abstract：:Mobilization of the T-cell response against cancer has the potential to achieve long-lasting cures. However, it is not known how to harness antigen-presenting cells optimally to achieve an effective antitumor T-cell response. In this study, we show that anti-CD47 antibody-mediated phagocytosis of cancer by macrophages...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tseng D,Volkmer JP,Willingham SB,Contreras-Trujillo H,Fathman JW,Fernhoff NB,Seita J,Inlay MA,Weiskopf K,Miyanishi M,Weissman IL

更新日期：2013-07-02 00:00:00

abstract：:Little is known about the molecular mechanisms by which the embryo proper and suspensor of plant embryos activate specific gene sets shortly after fertilization. We analyzed the upstream region of the scarlet runner bean (Phaseolus coccineus) G564 gene to understand how genes are activated specifically within the susp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawashima T,Wang X,Henry KF,Bi Y,Weterings K,Goldberg RB

更新日期：2009-03-03 00:00:00

abstract：:The detection, stabilization, and repair of stress-induced damage are essential requirements for cellular life. All cells respond to osmotic stress-induced water loss with increased expression of genes that mediate accumulation of organic osmolytes, solutes that function as chemical chaperones and restore osmotic home...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lamitina T,Huang CG,Strange K

更新日期：2006-08-08 00:00:00

abstract：:Cointegrate DNA molecules containing two copies of a transposable element appear to be intermediates in the transposition process. These structures are resolved by site-specific recombination to yield the normal end products of transposition. The transposable element gamma delta (Tn1000) synthesizes a product intercha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kitts P,Symington L,Burke M,Reed R,Sherratt D

更新日期：1982-01-01 00:00:00

abstract：:RNA-protein interactions underlie a wide range of cellular processes. Improved methods are needed to systematically map RNA-protein interactions in living cells in an unbiased manner. We used two approaches to target the engineered peroxidase APEX2 to specific cellular RNAs for RNA-centered proximity biotinylation of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han S,Zhao BS,Myers SA,Carr SA,He C,Ting AY

更新日期：2020-09-08 00:00:00

abstract：:Two subjects, of 11,000 healthy individuals screened, were found to be missing three and four immunoglobulin isotypes, respectively (IgA1, IgG2, and IgG4; IgA1, IgG2, IgG4, and IgE), and have been analyzed at the DNA level by means of Southern blotting and Ig heavy-chain-specific probes. A broad deletion within the he...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Migone N,Oliviero S,de Lange G,Delacroix DL,Boschis D,Altruda F,Silengo L,DeMarchi M,Carbonara AO

更新日期：1984-09-01 00:00:00

abstract：:Ligand-specific molecular switches composed of RNA were created by coupling preexisting catalytic and receptor domains via structural bridges. Binding of ligand to the receptor triggers a conformational change within the bridge, and this structural reorganization dictates the activity of the adjoining ribozyme. The mo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Soukup GA,Breaker RR

更新日期：1999-03-30 00:00:00

abstract：:Understanding the ecological impacts of climate change is a crucial challenge of the twenty-first century. There is a clear lack of general rules regarding the impacts of global warming on biota. Here, we present a metaanalysis of the effect of climate change on body size of ectothermic aquatic organisms (bacteria, ph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Daufresne M,Lengfellner K,Sommer U

更新日期：2009-08-04 00:00:00

abstract：:The six individual catalytic polypeptide chains within the two catalytic trimers of Escherichia coli aspartate transcarbamoylase (ATCase; EC 2.1.3.2) are folded into two discrete structural domains interconnected in part by helix 12, which comprises residues 285-305 and is located near the carboxyl terminus of the cha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peterson CB,Schachman HK

更新日期：1991-01-15 00:00:00

abstract：:There exists a properly embedded minimal surface of genus one with a single end asymptotic to the end of the helicoid. This genus-one helicoid is constructed as the limit of a continuous one-parameter family of screw-motion invariant minimal surfaces, also asymptotic to the helicoid, that have genus equal to one in th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weber M,Hoffman D,Wolf M

更新日期：2005-11-15 00:00:00

abstract：:Basal ganglia-thalamocortical circuits are critical for motor control and motor learning. Classically, basal ganglia nuclei are thought to regulate motor behavior by increasing or decreasing cortical firing rates, and basal ganglia diseases are assumed to reflect abnormal overall activity levels. More recent studies s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kojima S,Kao MH,Doupe AJ

更新日期：2013-03-19 00:00:00

abstract：:Most, but not all, aged rats exhibit a profound deficit in spatial memory when tested in a radial maze--a task known to depend on the integrity of the hippocampal formation. In this study, animals were divided into three groups based on their spatial memory capacity: young adult rats with good memory, aged rats with i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Geinisman Y,de Toledo-Morrell L,Morrell F

更新日期：1986-05-01 00:00:00

abstract：:This paper describes the state of the art in applications of voice-processing technologies. In the first part, technologies concerning the implementation of speech recognition and synthesis algorithms are described. Hardware technologies such as microprocessors and DSPs (digital signal processors) are discussed. Softw...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakatsu R,Suzuki Y

更新日期：1995-10-24 00:00:00

abstract：:We constructed a comprehensive cDNA library from HeLa cell mRNA in a vector that directs expression of the cDNA in Saccharomyces cerevisiae. We used this library to clone the human counterpart of the Sa. cerevisiae CCAAT-binding transcription factor, Hap2, by functional complementation of a hap2 mutation. The cDNA enc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Becker DM,Fikes JD,Guarente L

更新日期：1991-03-01 00:00:00

abstract：:The structural features that determine tRNA(Ala) acceptor identity have been studied with amber-suppressor tRNAs in Escherichia coli cells. Previous work established that a wobble pair composed of guanosine at position 3 and uridine at position 70 (G3-U70) in the acceptor helix of tRNA(Ala) is a determinant of the mol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McClain WH,Foss K,Jenkins RA,Schneider J

更新日期：1991-10-15 00:00:00

abstract：:Antimicrobial peptides constitute an important component of the mammalian innate immune response. Several types of antimicrobial peptides, including the beta-defensins, are produced at epithelial surfaces in response to infectious threats. Here we show that a class of small molecules, including l-isoleucine and severa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fehlbaum P,Rao M,Zasloff M,Anderson GM

更新日期：2000-11-07 00:00:00