Solution structure and dynamics of a de novo designed three-helix bundle protein.

abstract：:The D1 reaction center protein of photosystem II (PSII) is subject to light-induced damage. Degradation of damaged D1 and its replacement by nascent D1 are at the heart of a PSII repair cycle, without which photosynthesis is inhibited. In mature plant chloroplasts, light stimulates the recruitment of ribosomes specifi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chotewutmontri P,Barkan A

更新日期：2020-09-01 00:00:00

abstract：:There is a tightly coupled bidirectional interaction between visual cortex and visual thalamus [lateral geniculate nucleus (LGN)]. Using drifting sinusoidal grating stimuli, we compared the response of cells in the LGN with and without feedback from the visual cortex. Raster plots revealed a striking difference in the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Andolina IM,Jones HE,Wang W,Sillito AM

更新日期：2007-01-30 00:00:00

abstract：:Exchange proteins directly activated by cAMP (Epac1 and Epac2) have been recently recognized as key players in β-adrenergic-dependent cardiac arrhythmias. Whereas Epac1 overexpression can lead to cardiac hypertrophy and Epac2 activation can be arrhythmogenic, it is unknown whether distinct subcellular distribution of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pereira L,Rehmann H,Lao DH,Erickson JR,Bossuyt J,Chen J,Bers DM

更新日期：2015-03-31 00:00:00

abstract：:Epilepsy is characterized by recurrent synchronizations of neuronal activity, which are both a cardinal clinical symptom and a debilitating phenomenon. Although the temporal dynamics of epileptiform synchronizations are well described at the macroscopic level using electrophysiological approaches, less is known about ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feldt Muldoon S,Soltesz I,Cossart R

更新日期：2013-02-26 00:00:00

abstract：:The generation of diverse neuronal types and subtypes from multipotent progenitors during development is crucial for assembling functional neural circuits in the adult central nervous system. During mouse retinogenesis, early retinal progenitors give rise to several cell types, including ganglion, amacrine, horizontal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luo H,Jin K,Xie Z,Qiu F,Li S,Zou M,Cai L,Hozumi K,Shima DT,Xiang M

更新日期：2012-02-28 00:00:00

abstract：:Phosphorylation-based signaling pathways employ dephosphorylation mechanisms for signal termination. Histidine to aspartate phosphosignaling in the two-component system that controls bacterial chemotaxis has been studied extensively. Rhodobacter sphaeroides has a complex chemosensory pathway with multiple homologues o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Porter SL,Roberts MA,Manning CS,Armitage JP

更新日期：2008-11-25 00:00:00

abstract：:The transcription factor caudal-type homeobox 1 (CDX1) is a key regulator of differentiation in the normal colon and in colorectal cancer (CRC). CDX1 activates the expression of enterocyte genes, but it is not clear how the concomitant silencing of stem cell genes is achieved. MicroRNAs (miRNAs) are important mediator...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jones MF,Hara T,Francis P,Li XL,Bilke S,Zhu Y,Pineda M,Subramanian M,Bodmer WF,Lal A

更新日期：2015-03-31 00:00:00

abstract：:Immunological memory is a hallmark of adaptive immunity, and understanding T cell memory will be central to the development of effective cell-mediated vaccines. The characteristics and functions of CD4 memory cells have not been well defined. Here we demonstrate that the increased size of the secondary response is sol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： MacLeod MK,McKee A,Crawford F,White J,Kappler J,Marrack P

更新日期：2008-09-23 00:00:00

abstract：:Understanding the function of high-density lipoprotein (HDL) requires detailed knowledge of the structure of its primary protein, apolipoprotein A-I (APOA1). However, APOA1 flexibility and HDL heterogeneity have confounded decades of efforts to determine high-resolution structures and consistent models. Here, molecula...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pourmousa M,Song HD,He Y,Heinecke JW,Segrest JP,Pastor RW

更新日期：2018-05-15 00:00:00

abstract：:We have identified and isolated a presumptive leukemogenic provirus from myeloblasts of a chicken in which leukemia had been induced by avian myeloblastosis virus (AMV). Leukemic myeloblasts isolated from peripheral blood or from converted yolk sac cultures of various strains of chickens, regardless of the endogenous ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Souza LM,Komaromy MC,Baluda MA

更新日期：1980-05-01 00:00:00

abstract：:The type II cell of the alveolar epithelium of adult rats has been studied by electron microscopy to determine its ability to endocytose electron-dense tracers of differing molecular charge and size. The tracers studied were native (anionic) ferritin, cationic ferritin, 70-kilodalton dextran, and colloidal carbon. Alv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams MC

更新日期：1984-10-01 00:00:00

abstract：:Hematopoietic stem/progenitor cells (HSPCs) possess the potentials of self-renewal, proliferation, and differentiation toward different lineages of blood cells. These cells not only play a primordial role in hematopoietic development but also have important clinical application. Characterization of the gene expression...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mao M,Fu G,Wu JS,Zhang QH,Zhou J,Kan LX,Huang QH,He KL,Gu BW,Han ZG,Shen Y,Gu J,Yu YP,Xu SH,Wang YX,Chen SJ,Chen Z

更新日期：1998-07-07 00:00:00

abstract：:The sparking of cut grape hemispheres in a household microwave oven has been a poorly explained Internet parlor trick for over two decades. By expanding this phenomenon to whole spherical dimers of various grape-sized fruit and hydrogel water beads, we demonstrate that the formation of plasma is due to electromagnetic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Khattak HK,Bianucci P,Slepkov AD

更新日期：2019-03-05 00:00:00

abstract：:Identifying potentially unique features of the human cerebral cortex is a first step to understanding how evolution has shaped the brain in our species. By analyzing MR images obtained from 177 humans and 73 chimpanzees, we observed a human-specific asymmetry in the superior temporal sulcus at the heart of the communi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leroy F,Cai Q,Bogart SL,Dubois J,Coulon O,Monzalvo K,Fischer C,Glasel H,Van der Haegen L,Bénézit A,Lin CP,Kennedy DN,Ihara AS,Hertz-Pannier L,Moutard ML,Poupon C,Brysbaert M,Roberts N,Hopkins WD,Mangin JF,Dehaene-

更新日期：2015-01-27 00:00:00

abstract：:The N-terminal 392 amino acids of the Drosophila kinesin alpha subunit (designated DKH392) form a dimer in solution that releases only one of its two tightly bound ADP molecules on association with a microtubule, whereas a shorter monomeric construct (designated DKH340) releases > or = 95% of its one bound ADP on asso...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hackney DD

更新日期：1994-07-19 00:00:00

abstract：:Diminished Sonic Hedgehog (Shh) signaling is associated with the most common forebrain defect in humans, holoprosencephaly (HPE), which includes cyclopia, a phenotype also seen in mice and other vertebrates with defective Shh signaling. The secreted protein Shh acts as a crucial factor that patterns the ventral forebr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roessler E,Du YZ,Mullor JL,Casas E,Allen WP,Gillessen-Kaesbach G,Roeder ER,Ming JE,Ruiz i Altaba A,Muenke M

更新日期：2003-11-11 00:00:00

abstract：:We have investigated the regulation of the expression of two growth factors found in vascular smooth muscle, transforming growth factor alpha (TGF alpha) and basic fibroblast growth factor (bFGF). Cells cultured in medium containing 30 mM glucose exhibited a 2-fold increase in TGF alpha mRNA and a 3-fold increase in b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McClain DA,Paterson AJ,Roos MD,Wei X,Kudlow JE

更新日期：1992-09-01 00:00:00

abstract：:The pancreatic beta cell can respond in the long term to hyperglycemia both with an increased capacity for insulin production and, in susceptible individuals, with apoptosis. When glucose-induced apoptosis offsets the increasing beta cell capacity, type 2 diabetes results. Here, we tested the idea that the pathway of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu K,Paterson AJ,Chin E,Kudlow JE

更新日期：2000-03-14 00:00:00

abstract：:Concepts organize the relationship among individual stimuli or events by highlighting shared features. Often, new goals require updating conceptual knowledge to reflect relationships based on different goal-relevant features. Here, our aim is to determine how hippocampal (HPC) object representations are organized and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mack ML,Love BC,Preston AR

更新日期：2016-11-15 00:00:00

abstract：:To investigate protein folding dynamics in terms of compactness, we developed a continuous-flow mixing device to make small-angle x-ray scattering measurements with the time resolution of 160 micros and characterized the radius of gyration (R(g)) of two folding intermediates of cytochrome c (cyt c). The early intermed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Akiyama S,Takahashi S,Kimura T,Ishimori K,Morishima I,Nishikawa Y,Fujisawa T

更新日期：2002-02-05 00:00:00

abstract：:The role of the mouse tumor necrosis factor (TNF) promoter, 5' untranslated region (UTR), and 3' UTR in TNF gene expression has been examined in three nonmacrophage cell lines (HeLa, NIH 3T3, and L-929). The TNF promoter is not macrophage-specific. On the contrary, it constitutively drives reporter gene expression in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kruys V,Kemmer K,Shakhov A,Jongeneel V,Beutler B

更新日期：1992-01-15 00:00:00

abstract：:Effects of thyrotropin-releasing hormone (TRH; thyroliberin) on membrane electrical properties were studied in the clonal rat anterior pituitary cell (GH3) by continuous recording of the intracellular potential. Application of TRH, which stimulates the release of prolactin and growth hormone from the GH3 cell, elicite...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ozawa S,Kimura N

更新日期：1979-11-01 00:00:00

abstract：:The thymidine analog fialuridine deoxy-2-fluoro-beta-D-arabinofuranosyl)-5-iodouracil (FIAU) was toxic in trials for chronic hepatitis B infection. One mechanism postulated that defective mtDNA replication was mediated through inhibition of DNA polymerase-gamma (DNA pol-gamma), by FIAU triphosphate (FIALTP) or by trip...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lewis W,Levine ES,Griniuviene B,Tankersley KO,Colacino JM,Sommadossi JP,Watanabe KA,Perrino FW

更新日期：1996-04-16 00:00:00

abstract：:Morphological and genetic characters from cyprinid fishes of the genus Gila were examined to assess a hypothesized hybrid origin of Gila seminuda from the Virgin River, Arizona-Nevada-Utah. The presumed parents, Gila robusta robusta and Gila elegans, are clearly differentiated from one another based on morphology, all...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DeMarais BD,Dowling TE,Douglas ME,Minckley WL,Marsh PC

更新日期：1992-04-01 00:00:00

abstract：:Mutations at the mouse limb deformity (ld) locus result in defects of growth and patterning of the limb and kidney during embryonic development. The gene responsible for this phenotype is large and complex, with the capacity to generate a number of alternatively spliced messenger RNA transcripts encoding nuclear prote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vogt TF,Jackson-Grusby L,Rush J,Leder P

更新日期：1993-06-15 00:00:00

abstract：:Healthy ocean ecosystems are needed to sustain people and livelihoods and to achieve the United Nations Sustainable Development Goals. Using the ocean sustainably requires overcoming many formidable challenges: overfishing, climate change, ocean acidification, and pollution. Despite gloomy forecasts, there is reason f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lubchenco J,Cerny-Chipman EB,Reimer JN,Levin SA

更新日期：2016-12-20 00:00:00

abstract：:beta1,4-Galactosyltransferase (beta4GalT-I) participates in both glycoconjugate biosynthesis (ubiquitous activity) and lactose biosynthesis (mammary gland-specific activity). In somatic tissues, transcription of the mammalian beta4GalT-I gene results in a 4.1-kb mRNA and a 3.9-kb mRNA as a consequence of initiation at...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Charron M,Shaper JH,Shaper NL

更新日期：1998-12-08 00:00:00

abstract：:Uncoupling protein 1 (UCP1) catalyzes fatty acid-activated, purine nucleotide-sensitive proton leak across the mitochondrial inner membrane of brown adipose tissue to produce heat, and could help combat obesity and metabolic disease in humans. Studies over the last 30 years conclude that the protein is a dimer, bindin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee Y,Willers C,Kunji ER,Crichton PG

更新日期：2015-06-02 00:00:00

abstract：:The ATP-dependent enzyme dethiobiotin synthetase from Escherichia coli catalyses the formation of dethiobiotin from CO2 and 7, 8-diaminopelargonic acid. The reaction is initiated by the formation of a carbamate and proceeds through a phosphorylated intermediate, a mixed carbamic phosphoric anhydride. Here, we report t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Käck H,Gibson KJ,Lindqvist Y,Schneider G

更新日期：1998-05-12 00:00:00

abstract：:The qualities of perception depend not only on the sensory inputs but also on the brain state before stimulus presentation. Although the collective evidence from neuroimaging studies for a relation between prestimulus state and perception is strong, the interpretation in the context of sensory computations or decision...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kayser SJ,McNair SW,Kayser C

更新日期：2016-04-26 00:00:00