Eradication of large colon tumor xenografts by targeted delivery of maytansinoids.

abstract：:Thymidine kinase-deficient mouse L cells have been transformed with plasmid DNAs carrying 8-base-pair Xho I linker insertion mutations in the coding region of the herpes simplex virus type 1 thymidine kinase gene. When the mutant plasmids are introduced individually into LTK- cells, transformation efficiencies are gre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shapira G,Stachelek JL,Letsou A,Soodak LK,Liskay RM

更新日期：1983-08-01 00:00:00

abstract：:Genetically engineered mice with targeted disruption of the neuronal nitric oxide synthase (nNOS) gene established the inhibitory role of nitric oxide (NO) in male impulsive aggressive behavior. This was later confirmed by using selective nNOS inhibitors in male wild-type mice. The molecular mechanisms accounting for ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chiavegatto S,Dawson VL,Mamounas LA,Koliatsos VE,Dawson TM,Nelson RJ

更新日期：2001-01-30 00:00:00

abstract：:Expression of glucokinase in hepatocytes and pancreatic 6-cells is of major physiologic importance to mammalian glucose homeostasis. Liver glucokinase catalyzes the first committed step in the disposal of glucose, and beta-cell glucokinase catalyzes a rate-limiting step required for glucose-regulated insulin release. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heimberg H,De Vos A,Moens K,Quartier E,Bouwens L,Pipeleers D,Van Schaftingen E,Madsen O,Schuit F

更新日期：1996-07-09 00:00:00

abstract：:In mammals and insects, pheromones strongly influence social behaviors such as aggression and mate recognition. In Drosophila melanogaster, pheromones in the form of cuticular hydrocarbons play prominent roles in courtship. GC/MS is the primary analytical tool currently used to study Drosophila cuticular hydrocarbons....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yew JY,Cody RB,Kravitz EA

更新日期：2008-05-20 00:00:00

abstract：:The increasing prevalence of antibiotic-resistant pathogens reinforces the need for structures of antibiotic-ribosome complexes that are accurate enough to enable the rational design of novel ribosome-targeting therapeutics. Structures of many antibiotics in complex with both archaeal and eubacterial ribosomes have be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bulkley D,Innis CA,Blaha G,Steitz TA

更新日期：2010-10-05 00:00:00

abstract：:Mind wandering represents the human capacity for internally focused thought and relies upon the brain's default network and its interactions with attentional networks. Studies have characterized mind wandering in healthy people, yet there is limited understanding of how this capacity is affected in clinical population...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Callaghan C,Shine JM,Hodges JR,Andrews-Hanna JR,Irish M

更新日期：2019-02-19 00:00:00

abstract：:Previous studies have shown that the oxidants Cu(++), H(2)O(2), and diamide mimic the stimulatory effect of insulin on 3-O-methylglucose transport in isolated fat cells. The present experiments were designed to determine whether sulfhydryl oxidation plays a key role in the activation of the glucose transport system. I...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Czech MP,Lawrence JC Jr,Lynn WS

更新日期：1974-10-01 00:00:00

abstract：:Gating currents of the cloned delayed-rectifier K+ channel DRK1 expressed in Xenopus oocytes were measured with the open-oocyte Vaseline-gap voltage-clamp technique. DRK1 gating charge had the following salient properties: (i) gating-charge amplitude correlated positively with size of the expressed ionic K+ currents; ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Taglialatela M,Stefani E

更新日期：1993-05-15 00:00:00

abstract：:A gene-transfer approach was used to explore the function of the BCL2 (B-cell lymphoma/leukemia 2) gene in a human T-cell line, Jurkat. Though stable introduction of a BCL2 expression plasmid into Jurkat T cells was by itself insufficient, the combined transfer of BCL2 and MYC genes markedly enhanced the tumorigenicit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reed JC,Cuddy M,Haldar S,Croce C,Nowell P,Makover D,Bradley K

更新日期：1990-05-01 00:00:00

abstract：:A major goal of tumor immunotherapy is the effective eradication of established metastases associated with the induction of a T cell-mediated protective immunity. We achieved this in a poorly immunogenic murine neuroblastoma model by gene therapy with a single chain interleukin 12 (scIL-12) fusion protein that assures...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lode HN,Dreier T,Xiang R,Varki NM,Kang AS,Reisfeld RA

更新日期：1998-03-03 00:00:00

abstract：:Many cell surface receptors are multimeric proteins, composed of several structural domains, some involved in ligand recognition, whereas others are responsible for signal transduction. In most cases, the mechanism of how ligand interaction in the extracellular domains leads to the activation of effector domains remai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang S,Cao J,Jiang M,Labesse G,Liu J,Pin JP,Rondard P

更新日期：2011-09-13 00:00:00

abstract：:Type I (IFN-alpha/beta) and type III (IFN-lambdas) IFNs are important components of the host antiviral response. Although type III IFNs possess intrinsic antiviral activity similar to that of type I IFNs, they signal through a specific unique receptor complex, and their functional importance for antiviral resistance i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang J,Smirnov SV,Lewis-Antes A,Balan M,Li W,Tang S,Silke GV,Pütz MM,Smith GL,Kotenko SV

更新日期：2007-06-05 00:00:00

abstract：:We established a pancreatic adenocarcinoma cell line (CFPAC-1) from a patient with cystic fibrosis (CF) and assessed some of its properties. The cells show epithelial morphology and express cytokeratin and oncofetal antigens characteristic of pancreatic duct cells. Basal and stimulated levels of cAMP and cAMP-dependen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schoumacher RA,Ram J,Iannuzzi MC,Bradbury NA,Wallace RW,Hon CT,Kelly DR,Schmid SM,Gelder FB,Rado TA

更新日期：1990-05-01 00:00:00

abstract：:Tropical cyclones have massive economic, social, and ecological impacts, and models of their occurrence influence many planning activities from setting insurance premiums to conservation planning. Most impact models allow for geographically varying cyclone rates but assume that individual storm events occur randomly w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mumby PJ,Vitolo R,Stephenson DB

更新日期：2011-10-25 00:00:00

abstract：:Sera from 11 individuals which were classified as Lp(a-) by direct gel diffusion and by absorption tests showed the presence of Lp(a) lipoprotein when the sera were concentrated 120-fold. This Lp(a) antigen was demonstrated by gel diffusion to be indistinguishable from Lp-lipoprotein isolated from known Lp(a+) sera. T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harvie NR,Schultz JS

更新日期：1970-05-01 00:00:00

abstract：:Analysis of the nucleotide and deduced amino acid sequences of the biologically active mouse mdr1 cDNA clone indicates that the protein is formed by two highly homologous halves, each containing six putative transmembrane domains and a nucleotide-binding site. The duplicated unit shows high sequence homology to the pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raymond M,Gros P

更新日期：1989-09-01 00:00:00

abstract：:Chlorarachniophytes are amoeboid algae with unusual chloroplasts. Instead of the usual two membranes that surround the chloroplasts of plants, green algae, and red algae, the chloroplasts of chlorarachniophytes have four bounding membranes. The extra membranes may reflect an unusual origin of chlorarachniophyte chloro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McFadden GI,Gilson PR,Hofmann CJ,Adcock GJ,Maier UG

更新日期：1994-04-26 00:00:00

abstract：:Legionella pneumophila is an intracellular parasite of freshwater protozoa and human macrophages. Recent studies determined that the macrophage infectivity potentiator (Mip) surface protein, a prokaryotic homolog of the FK506-binding proteins, is required for optimal infection of macrophages. To determine whether Mip ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cianciotto NP,Fields BS

更新日期：1992-06-01 00:00:00

abstract：:A tetramer of the Mu transposase (MuA) pairs the recombination sites, cleaves the donor DNA, and joins these ends to a target DNA by strand transfer. Juxtaposition of the recombination sites is accomplished by the assembly of a stable synaptic complex of MuA protein and Mu DNA. This initial critical step is facilitate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clubb RT,Mizuuchi M,Huth JR,Omichinski JG,Savilahti H,Mizuuchi K,Clore GM,Gronenborn AM

更新日期：1996-02-06 00:00:00

abstract：:From what viruses the human immunodeficiency viruses (HIVs) originated is an extremely controversial question. To address this question, we have analyzed nucleotide sequences of simian immunodeficiency viruses (SIVs) and HIVs by using the techniques for understanding molecular evolution. In particular, we compared the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gojobori T,Moriyama EN,Ina Y,Ikeo K,Miura T,Tsujimoto H,Hayami M,Yokoyama S

更新日期：1990-06-01 00:00:00

abstract：:The supposition that agricultural intensification results in land sparing for conservation has become central to policy formulations across the tropics. However, underlying assumptions remain uncertain and have been little explored in the context of conservation incentive schemes such as policies for Reducing Emission...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Phelps J,Carrasco LR,Webb EL,Koh LP,Pascual U

更新日期：2013-05-07 00:00:00

abstract：:Nodules harboring nitrogen-fixing rhizobia are a well-known trait of legumes, but nodules also occur in other plant lineages, with rhizobia or the actinomycete Frankia as microsymbiont. It is generally assumed that nodulation evolved independently multiple times. However, molecular-genetic support for this hypothesis ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van Velzen R,Holmer R,Bu F,Rutten L,van Zeijl A,Liu W,Santuari L,Cao Q,Sharma T,Shen D,Roswanjaya Y,Wardhani TAK,Kalhor MS,Jansen J,van den Hoogen J,Güngör B,Hartog M,Hontelez J,Verver J,Yang WC,Schijlen E,Repin

更新日期：2018-05-15 00:00:00

abstract：:The absorption spectrum of an activesite specific chromophoric acyl enzyme, sturgeon beta-(2-furyl)-acryloyl-glyceraldehyde-3-phosphate dehydrogenase, is reported. This acyl enzyme undergoes all of the catalyzed reactions characteristic of the intermediate of the physiological acyl enzyme, 3-phospho-D-glyceroyl-glycer...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Malhotra OP,Bernhard SA

更新日期：1973-07-01 00:00:00

abstract：:To examine the hypothesis that bone marrow consists of discrete stem cell regulatory volumes or domains, we studied spleen colony-forming unit (CFU-S) population growth kinetics in unirradiated WBB6F1-W/Wv mice receiving various doses of +/+ bone marrow cells. Assay of femoral marrow CFU-S content in the eight recipie...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maloney MA,Lamela RA,Banda MJ,Patt HM

更新日期：1982-02-01 00:00:00

abstract：:We study the physical origins of phase contrast in dynamic atomic force microscopy (dAFM) in liquids where low-stiffness microcantilever probes are often used for nanoscale imaging of soft biological samples with gentle forces. Under these conditions, we show that the phase contrast derives primarily from a unique ene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Melcher J,Carrasco C,Xu X,Carrascosa JL,Gómez-Herrero J,José de Pablo P,Raman A

更新日期：2009-08-18 00:00:00

abstract：:L-Gulono-gamma-lactone oxidase (L-gulono-gamma-lactone:oxygen 2-oxidoreductase, EC 1.1.3.8) is the enzyme that catalyzes the terminal step of L-ascorbic acid biosynthesis in mammalian liver. The absence of the oxidase activity in primates and guinea pigs is the reason why these animals are subject to scurvy, which mus...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nishikimi M,Udenfriend S

更新日期：1976-06-01 00:00:00

abstract：:Oncogenic mutations in the small GTPase KRAS are frequently found in human cancers, and, currently, there are no effective targeted therapies for these tumors. Using a combinatorial siRNA approach, we analyzed a panel of KRAS mutant colorectal and pancreatic cancer cell lines for their dependency on 28 gene nodes that...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee CS,Lee LC,Yuan TL,Chakka S,Fellmann C,Lowe SW,Caplen NJ,McCormick F,Luo J

更新日期：2019-03-05 00:00:00

abstract：:Solar emission produces copious nitrosonium ions (NO(+)) in the D layer of the ionosphere, 60 to 90 km above the Earth's surface. NO(+) is believed to transfer its charge to water clusters in that region, leading to the formation of gaseous nitrous acid (HONO) and protonated water cluster. The dynamics of this reactio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： He R,Li L,Zhong J,Zhu C,Francisco JS,Zeng XC

更新日期：2016-04-26 00:00:00

abstract：:T-cell tumors are characterized by inversions or translocations of chromosome 14. The breakpoints of these karyotypic abnormalities occur in chromosome bands 14q11 and 14q32--the same bands in which the T-cell receptor (TCR) alpha-chain and immunoglobulin heavy chain genes have been mapped, respectively. Patients with...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baer R,Heppell A,Taylor AM,Rabbitts PH,Boullier B,Rabbitts TH

更新日期：1987-12-01 00:00:00

abstract：:Arachidonic acid (AA) has recently been shown to influence various cellular functions in the central nervous system. Here we report that AA increases, in a time- and concentration-dependent manner, 2-deoxy-D-[1-3H]glucose ([3H]2DG) uptake in primary cultures of astrocytes prepared from the cerebral cortex of neonatal ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu N,Martin JL,Stella N,Magistretti PJ

更新日期：1993-05-01 00:00:00