Abstract:
:Thymidine kinase-deficient mouse L cells have been transformed with plasmid DNAs carrying 8-base-pair Xho I linker insertion mutations in the coding region of the herpes simplex virus type 1 thymidine kinase gene. When the mutant plasmids are introduced individually into LTK- cells, transformation efficiencies are greatly reduced relative to the wild type. However, when two mutant plasmids are cotransferred into the same LTK- recipients, significantly higher frequencies of transformation are observed (30-300 times). Here we demonstrate the usefulness of linker insertions for the study of homologous recombination in detecting the existence of normal thymidine kinase gene sequences (i.e., sequences lacking the insertions after recombination are substantiated by DNA . DNA hybridization). In addition, the frequencies of recombination in the various "crosses" are consistent with the known positions of the mutations.
journal_name
Proc Natl Acad Sci U S Aauthors
Shapira G,Stachelek JL,Letsou A,Soodak LK,Liskay RMdoi
10.1073/pnas.80.15.4827subject
Has Abstractpub_date
1983-08-01 00:00:00pages
4827-31issue
15eissn
0027-8424issn
1091-6490journal_volume
80pub_type
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