The wing of the enhancer-binding domain of Mu phage transposase is flexible and is essential for efficient transposition.

Abstract:

:A tetramer of the Mu transposase (MuA) pairs the recombination sites, cleaves the donor DNA, and joins these ends to a target DNA by strand transfer. Juxtaposition of the recombination sites is accomplished by the assembly of a stable synaptic complex of MuA protein and Mu DNA. This initial critical step is facilitated by the transient binding of the N-terminal domain of MuA to an enhancer DNA element within the Mu genome (called the internal activation sequence, IAS). Recently we solved the three-dimensional solution structure of the enhancer-binding domain of Mu phage transposase (residues 1-76, MuA76) and proposed a model for its interaction with the IAS element. Site-directed mutagenesis coupled with an in vitro transposition assay has been used to assess the validity of the model. We have identified five residues on the surface of MuA that are crucial for stable synaptic complex formation but dispensable for subsequent events in transposition. These mutations are located in the loop (wing) structure and recognition helix of the MuA76 domain of the transposase and do not seriously perturb the structure of the domain. Furthermore, in order to understand the dynamic behavior of the MuA76 domain prior to stable synaptic complex formation, we have measured heteronuclear 15N relaxation rates for the unbound MuA76 domain. In the DNA free state the backbone atoms of the helix-turn-helix motif are generally immobilized whereas the residues in the wing are highly flexible on the pico- to nanosecond time scale. Together these studies define the surface of MuA required for enhancement of transposition in vitro and suggest that a flexible loop in the MuA protein required for DNA recognition may become structurally ordered only upon DNA binding.

authors

Clubb RT,Mizuuchi M,Huth JR,Omichinski JG,Savilahti H,Mizuuchi K,Clore GM,Gronenborn AM

doi

10.1073/pnas.93.3.1146

subject

Has Abstract

pub_date

1996-02-06 00:00:00

pages

1146-50

issue

3

eissn

0027-8424

issn

1091-6490

journal_volume

93

pub_type

杂志文章
  • Synthetic hexapeptide substrates and inhibitors of 3':5'-cyclic AMP-dependent protein kinase.

    abstract::The substrate specificity of the catalytic subunit of rabbit skeletal muscle 3': 5'-cyclic AMP-dependent protein kinase (EC 2.7.1.37; ATP: protein phosphotransferase) has been studied using the synthetic peptide Arg-Gly-Tyr-Ser-Leu-Gly corresponding to the sequence around serine 24, a phosphorylation site in reduced, ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.73.4.1038

    authors: Kemp BE,Benjamini E,Krebs EG

    更新日期:1976-04-01 00:00:00

  • Recreational fishing selectively captures individuals with the highest fitness potential.

    abstract::Fisheries-induced evolution and its impact on the productivity of exploited fish stocks remains a highly contested research topic in applied fish evolution and fisheries science. Although many quantitative models assume that larger, more fecund fish are preferentially removed by fishing, there is no empirical evidence...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1212536109

    authors: Sutter DA,Suski CD,Philipp DP,Klefoth T,Wahl DH,Kersten P,Cooke SJ,Arlinghaus R

    更新日期:2012-12-18 00:00:00

  • Elevated serum ribonuclease in patients with pancreatic cancer.

    abstract::Serum RNase (ribonuclease) of normal persons and of patients with pancreatitis, carcinoma of pancreas, or other neoplasms was determined with poly(C) as substrate. Strikingly abnormal elevations occur in the serum RNase of patients with pancreatic cancer. There is no elevation in the serum RNase level of patients with...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.73.7.2308

    authors: Reddi KK,Holland JF

    更新日期:1976-07-01 00:00:00

  • Concerted biosynthesis of an insect elicitor of plant volatiles.

    abstract::A variety of agricultural plant species, including corn, respond to insect herbivore damage by releasing large quantities of volatile compounds and, as a result, become highly attractive to parasitic wasps that attack the herbivores. An elicitor of plant volatiles, N-(17-hydroxylinolenoyl)-L-glutamine, named volicitin...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.95.23.13971

    authors: Paré PW,Alborn HT,Tumlinson JH

    更新日期:1998-11-10 00:00:00

  • Deletions affecting the transposition of an antibiotic resistance gene.

    abstract::The structural gene for plasmid-mediated ampicillin resistance resides upon a 3.2 X 10(6) dalton transposable sequence (TnA) flanked by short inverted repeated sequences that accompany its insertion. TnA was transposed to pMB8, a 1.8 X 10(6) dalton derivative of the colicingenic plasmid ColE1. Random deletions were in...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.74.2.702

    authors: Heffron F,Bedinger P,Champoux JJ,Falkow S

    更新日期:1977-02-01 00:00:00

  • Central role for hydrogen peroxide in P2Y1 ADP receptor-mediated cellular responses in vascular endothelium.

    abstract::ADP activates a family of cell surface receptors that modulate signaling pathways in a broad range of cells. ADP receptor antagonists are widely used to treat cardiovascular disease states. These studies identify a critical role for the stable reactive oxygen species hydrogen peroxide (H2O2) in mediating cellular resp...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1320854111

    authors: Kalwa H,Sartoretto JL,Martinelli R,Romero N,Steinhorn BS,Tao M,Ozaki CK,Carman CV,Michel T

    更新日期:2014-03-04 00:00:00

  • Urea-inducible Egr-1 transcription in renal inner medullary collecting duct (mIMCD3) cells is mediated by extracellular signal-regulated kinase activation.

    abstract::Urea (200-400 milliosmolar) activates transcription, translation of, and trans-activation by the immediate-early gene transcription factor Egr-1 in a renal epithelial cell-specific fashion. The effect at the transcriptional level has been attributed to multiple serum response elements and their adjacent Ets motifs loc...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.20.11242

    authors: Cohen DM

    更新日期:1996-10-01 00:00:00

  • Enzymatic block in the synthesis of gangliosides in DNA virus-transformed tumorigenic mouse cell lines.

    abstract::The ganglioside pattern of both SV40- and polyoma virus-transformed mouse cell lines differs from that of the parent cell lines or of cell lines that have transformed spontaneously in tissue culture. This is manifested by a dramatic decrease of gangliosides with an oligosaccharide chain larger than sialyllactose. Pres...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.67.2.757

    authors: Cumar FA,Brady RO,Kolodny EH,McFarland VW,Mora PT

    更新日期:1970-10-01 00:00:00

  • Decision versus compromise for animal groups in motion.

    abstract::Previously, we showed using a computational agent-based model that a group of animals moving together can make a collective decision on direction of motion, even if there is a conflict between the directional preferences of two small subgroups of "informed" individuals and the remaining "uninformed" individuals have n...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1118318108

    authors: Leonard NE,Shen T,Nabet B,Scardovi L,Couzin ID,Levin SA

    更新日期:2012-01-03 00:00:00

  • Structure of the Plasmodium-interspersed repeat proteins of the malaria parasite.

    abstract::The deadly symptoms of malaria occur as Plasmodium parasites replicate within blood cells. Members of several variant surface protein families are expressed on infected blood cell surfaces. Of these, the largest and most ubiquitous are the Plasmodium-interspersed repeat (PIR) proteins, with more than 1,000 variants in...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2016775117

    authors: Harrison TE,Reid AJ,Cunningham D,Langhorne J,Higgins MK

    更新日期:2020-12-15 00:00:00

  • Phosphoglucose isomerase: a ketol isomerase with aldol C2-epimerase activity.

    abstract::With 13C NMR, phosphoglucose isomerase (PGI; D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) is shown to produce mannose 6-phosphate (M6P) slowly from a much more rapidly catalyzed equilibrium between glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). The identity of M6P and its formation from G6P plus F6P a...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.90.4.1237

    authors: Seeholzer SH

    更新日期:1993-02-15 00:00:00

  • Identification of Id4 as a regulator of BRCA1 expression by using a ribozyme-library-based inverse genomics approach.

    abstract::Expression of the breast and ovarian cancer susceptibility gene BRCA1 is down-regulated in sporadic breast and ovarian cancer cases. Therefore, the identification of genes involved in the regulation of BRCA1 expression might lead to new insights into the pathogenesis and treatment of these tumors. In the present study...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.98.1.130

    authors: Beger C,Pierce LN,Kruger M,Marcusson EG,Robbins JM,Welcsh P,Welch PJ,Welte K,King MC,Barber JR,Wong-Staal F

    更新日期:2001-01-02 00:00:00

  • Functional role for vasoactive intestinal polypeptide in the caudate nucleus: a 2-deoxy[14C]glucose investigation.

    abstract::The quantitative autoradiographic 2-deoxy-[14C]glucose technique has been used with conscious rats to investigate the functional consequences (reflected as alterations in local rates of glucose utilization) of unilateral intrastriatal administration of vasoactive intestinal polypeptide. Intrastriatal administration of...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.80.5.1472

    authors: McCulloch J,Kelly PA,Uddman R,Edvinsson L

    更新日期:1983-03-01 00:00:00

  • Essential role of phospholipase A2 activity in endothelial cell-induced modification of low density lipoprotein.

    abstract::Previous studies have established that incubation of low density lipoprotein (LDL) with cultured endothelial cells (EC) converts it to a new form (EC-modified LDL) that is now recognized by a specific receptor on macrophages (the acetyl LDL receptor) and is taken up and degraded 3-10 times more rapidly than native LDL...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.82.9.3000

    authors: Parthasarathy S,Steinbrecher UP,Barnett J,Witztum JL,Steinberg D

    更新日期:1985-05-01 00:00:00

  • Asymmetric cryo-EM structure of the canonical Allolevivirus Qβ reveals a single maturation protein and the genomic ssRNA in situ.

    abstract::Single-stranded (ss) RNA viruses infect all domains of life. To date, for most ssRNA virions, only the structures of the capsids and their associated protein components have been resolved to high resolution. Qβ, an ssRNA phage specific for the conjugative F-pilus, has a T = 3 icosahedral lattice of coat proteins assem...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1609482113

    authors: Gorzelnik KV,Cui Z,Reed CA,Jakana J,Young R,Zhang J

    更新日期:2016-10-11 00:00:00

  • Lateral mobility of band 3 in the human erythrocyte membrane studied by fluorescence photobleaching recovery: evidence for control by cytoskeletal interactions.

    abstract::Band 3, the major intrinsic protein of the human erythrocyte membrane, was specifically labeled with the covalent fluorescent probe eosin isothiocyanate. The lateral mobility of labeled band 3 in the plane of the membrane under various conditions of ionic strength and temperature was examined by using the fluorescence...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.77.5.2537

    authors: Golan DE,Veatch W

    更新日期:1980-05-01 00:00:00

  • Human herpesvirus-6 entry into the central nervous system through the olfactory pathway.

    abstract::Viruses have been implicated in the development of neurodegenerative diseases, such as Alzheimer's, Parkinson's, and multiple sclerosis. Human herpesvirus-6 (HHV-6) is a neurotropic virus that has been associated with a wide variety of neurologic disorders, including encephalitis, mesial temporal lobe epilepsy, and mu...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1105143108

    authors: Harberts E,Yao K,Wohler JE,Maric D,Ohayon J,Henkin R,Jacobson S

    更新日期:2011-08-16 00:00:00

  • Genes in one megabase of the HLA class I region.

    abstract::To define the gene content of the HLA class I region, cDNA selection was applied to three overlapping yeast artificial chromosomes (YACs) that spanned 1 megabase (Mb) of this region of the human major histocompatibility complex. These YACs extended from the region centromeric to HLA-E to the region telomeric to HLA-F....

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.90.24.11870

    authors: Wei H,Fan WF,Xu H,Parimoo S,Shukla H,Chaplin DD,Weissman SM

    更新日期:1993-12-15 00:00:00

  • Oligosaccharide modification by swainsonine treatment inhibits pulmonary colonization by B16-F10 murine melanoma cells.

    abstract::Oligosaccharide moieties of cell-surface glycoconjugates are thought to be involved in recognition events associated with tumor metastasis and invasion. Using swainsonine (SW), an inhibitor of Golgi alpha-mannosidase II that results in the formation of hybrid-type oligosaccharides on N-linked glycoproteins, we have te...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.83.6.1752

    authors: Humphries MJ,Matsumoto K,White SL,Olden K

    更新日期:1986-03-01 00:00:00

  • The LEF1/beta -catenin complex activates movo1, a mouse homolog of Drosophila ovo required for epidermal appendage differentiation.

    abstract::Drosophila ovo/svb (dovo) is required for epidermal cuticle/denticle differentiation and is genetically downstream of the wg signaling pathway. Similarly, a mouse homolog of dovo, movo1, is required for the proper formation of hair, a mammalian epidermal appendage. Here, we provide biochemical evidence that movo1 enco...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.092137099

    authors: Li B,Mackay DR,Dai Q,Li TW,Nair M,Fallahi M,Schonbaum CP,Fantes J,Mahowald AP,Waterman ML,Fuchs E,Dai X

    更新日期:2002-04-30 00:00:00

  • Disruption of quercetin metabolism by fungicide affects energy production in honey bees (Apis mellifera).

    abstract::Cytochrome P450 monooxygenases (P450) in the honey bee, Apis mellifera, detoxify phytochemicals in honey and pollen. The flavonol quercetin is found ubiquitously and abundantly in pollen and frequently at lower concentrations in honey. Worker jelly consumed during the first 3 d of larval development typically contains...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1614864114

    authors: Mao W,Schuler MA,Berenbaum MR

    更新日期:2017-03-07 00:00:00

  • Anti-idiotypic antibodies to anti-HLA receptors induced by pregnancy.

    abstract::We have previously shown that lymphoblasts alloactivated in vitro acquire the capacity of stimulating the autologous mixed lymphocyte response. This response is anti-idiotypic in nature because lymphocytes so primed display accelerated memory responses only when restimulated by autologous lymphoblasts that have been a...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.80.3.830

    authors: Suciu-Foca N,Reed E,Rohowsky C,Kung P,King DW

    更新日期:1983-02-01 00:00:00

  • Radiocarbon dates on bones of extinct birds from Hawaii.

    abstract::Bones from a stratified sedimentary deposit in the Puu Naio Cave site on Maui, Hawaiian Islands, reveal the late Holocene extinction of 19 species of birds. The age of the sediment and associated fauna was determined by direct radiocarbon dating (tandem particle accelerator-mass spectrometer; TAMS) of amino acids extr...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.84.8.2350

    authors: James HF,Stafford TW Jr,Steadman DW,Olson SL,Martin PS,Jull AJ,McCoy PC

    更新日期:1987-04-01 00:00:00

  • Early evidence (ca. 12,000 B.P.) for feasting at a burial cave in Israel.

    abstract::Feasting is one of humanity's most universal and unique social behaviors. Although evidence for feasting is common in the early agricultural societies of the Neolithic, evidence in pre-Neolithic contexts is more elusive. We found clear evidence for feasting on wild cattle and tortoises at Hilazon Tachtit cave, a Late ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1001809107

    authors: Munro ND,Grosman L

    更新日期:2010-08-31 00:00:00

  • Insulin release, insulin sensitivity, and glucose intolerance.

    abstract::Groups of subjects with different degrees of glucose intolerance were examined in order to determine, first, the capacity of the beta cells to release insulin upon glucose stimulation and, second, sensitivity to insulin. The groups were selected on the basis of fasting blood glucose value and tolerances to oral and in...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.77.12.7425

    authors: Efendić S,Wajngot A,Cerasi E,Luft R

    更新日期:1980-12-01 00:00:00

  • Class I MHC molecules on hematopoietic cells can support intrathymic positive selection of T cell receptor transgenic T cells.

    abstract::The identity of cells that mediate positive selection of CD8(+) T cells was investigated in two T cell receptor (TCR) transgenic systems. Irradiated beta(2)-microglobulin mutant mice or mice with mutations in both the K(b) and D(b) genes were repopulated with fetal liver cells from class I(+) TCR transgenic mice. In t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.96.20.11470

    authors: Zerrahn J,Volkmann A,Coles MC,Held W,Lemonnier FA,Raulet DH

    更新日期:1999-09-28 00:00:00

  • Glacial reduction and millennial-scale variations in Drake Passage throughflow.

    abstract::The Drake Passage (DP) is the major geographic constriction for the Antarctic Circumpolar Current (ACC) and exerts a strong control on the exchange of physical, chemical, and biological properties between the Atlantic, Pacific, and Indian Ocean basins. Resolving changes in the flow of circumpolar water masses through ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1509203112

    authors: Lamy F,Arz HW,Kilian R,Lange CB,Lembke-Jene L,Wengler M,Kaiser J,Baeza-Urrea O,Hall IR,Harada N,Tiedemann R

    更新日期:2015-11-03 00:00:00

  • Modification of DNA ends can decrease end joining relative to homologous recombination in mammalian cells.

    abstract::In animal cells, exogenous DNA recombines into random chromosomal sites much more frequently than it recombines into homologous sites. Free DNA ends are "recombinogenic" in both processes. To test the effects of specific ends on analogous extrachromosomal processes, we constructed a linear genome of simian virus 40 wi...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.84.14.4959

    authors: Chang XB,Wilson JH

    更新日期:1987-07-01 00:00:00

  • A nondefective (competent) adenovirus-SV40 hybrid isolated from the AD.2-SV40 hybrid population.

    abstract::A new nondefective hybrid virus has been plaque-isolated from the Ad.2-SV40 hybrid population. This virus replicates efficiently with one-hit kinetics in both human embryonic kidney and African green monkey kidney cells, induces an SV40 specific antigen which is detectable by immunofluorescence and complement-fixation...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.63.4.1128

    authors: Lewis AM Jr,Levin MJ,Wiese WH,Crumpacker CS,Henry PH

    更新日期:1969-08-01 00:00:00

  • Early and multiple origins of metastatic lineages within primary tumors.

    abstract::Many aspects of the evolutionary process of tumorigenesis that are fundamental to cancer biology and targeted treatment have been challenging to reveal, such as the divergence times and genetic clonality of metastatic lineages. To address these challenges, we performed tumor phylogenetics using molecular evolutionary ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1525677113

    authors: Zhao ZM,Zhao B,Bai Y,Iamarino A,Gaffney SG,Schlessinger J,Lifton RP,Rimm DL,Townsend JP

    更新日期:2016-02-23 00:00:00