Abstract:
:We have developed an enzyme-linked immunosorption assay (ELISA) with an antigen extract made by treatment of freeze-thawed sperm pools with 0.3 M lithium 3,5-diiodosalicylate (LIS). This procedure is quick, safe, and simple to perform in a clinical laboratory. When the LIS antigen preparation was compared by ELISA with whole sperm and several other antigen extracts, it was found to be superior in detecting human antisperm antibodies related to infertility; it gave less variability, better precision, and fewer false-positive reactions than whole sperm fixed to the wells as antigen. The antigen was stable when stored at -20 degrees C. In one experimental series, serum samples from 18 normal control subjects and 159 infertility patients with positive sperm-immobilizing titers, positive sperm-agglutinating titers, or both, were tested by ELISA with LIS antigen; the correlation yielded a P less than or equal to 0.0001. Based on another series of 156 samples from patients with positive antibody titers by the sperm agglutination test and 30 sera from normal humans, the predictive value of the test was 96%. In a third series of 162 serum samples obtained from 82 women and 80 men, we found no difference in the test results based on sex. The assay is specific for the antibody binding portion of the immunoglobulin. Absorption of positive sera with sperm but not red blood cells markedly reduces the response. In addition to being more efficient and precise than standard methods, the ELISA eliminates the need for fresh sperm and provides data that can be objectively interpreted.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Alexander NJ,Bearwood Ddoi
10.1016/s0015-0282(16)47603-xsubject
Has Abstractpub_date
1984-02-01 00:00:00pages
270-6issue
2eissn
0015-0282issn
1556-5653pii
S0015-0282(16)47603-Xjournal_volume
41pub_type
杂志文章abstract::A case is described in which, after a tubal sterilization procedure and subsequent unilateral tubal anastomosis, an ectopic gestation occurred in the contralateral, unrepaired, distal tubal segment. On the basis of this case and related evidence, the authors suggest that in cases in which only a unilateral tubal anast...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)59068-2
更新日期:1987-03-01 00:00:00
abstract:OBJECTIVE:To study the role of a synthetic insulin-like growth factor-I receptor (IGF-IR) antagonist, picropodophyllin, for mouse preimplantation embryo development in vivo and in vitro. DESIGN:In vitro and in vivo study. SETTING:Hospital-based research unit. ANIMALS:FVB/N mice and mouse embryos. INTERVENTION(S):Th...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2009.12.044
更新日期:2010-05-15 00:00:00
abstract::To explore the relationship between variation in AGT M235T gene and the development of the polycystic ovary syndrome (PCOS) and its sequelae, in the present study we evaluated AGT polymorphism M235T in women with PCOS and in a control group. Moreover, to detect any relationship between AGT M235T variation and intermed...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2005.05.043
更新日期:2005-11-01 00:00:00
abstract:OBJECTIVES:To compare the results of IVF cycles after coasting in patients treated with a GnRH antagonist or GnRH agonist protocol. DESIGN:A retrospective case-control study. SETTING:Infertility unit in a university-affiliated tertiary medical center. PATIENT(S):The study group included all women less than 38 years ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.11.086
更新日期:2009-02-01 00:00:00
abstract:OBJECTIVE:To summarize the procedures and outcomes of assisted reproductive technology (ART) initiated in the United States in 1994. DESIGN:Data were collected on the Society for Assisted Reproductive Technology (SART) Database program and submitted to KMPG Peat Marwick, who served as the 1994 collection center for th...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)58621-x
更新日期:1996-11-01 00:00:00
abstract:OBJECTIVE:To investigate whether a poor rate of implantation after in vitro fertilization (IVF) was due to poor embryonic/endometrial synchrony during the original IVF cycle, we have cryopreserved reinseminated-fertilized embryos for later more synchronous replacement after thawing. The chance of implantation of fresh ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)54725-6
更新日期:1991-12-01 00:00:00
abstract::In 80 normal puerperae, serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), including human chorionic gonadotropin (hCG/LH), and prolactin (PRL) levels were evaluated 6 to 29 hours after vaginal delivery. In these puerperae, PRL levels were higher and FSH levels were lower than in menstruating women; h...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)46103-0
更新日期:1982-03-01 00:00:00
abstract::Osteoporosis continues to be a major public health challenge in both women and men. There are more than 2 million low-impact fractures in the United States every year, 39% of which occur in men. The mortality rate in the first year after hip fracture is estimated at 20% to 25% in women and is even higher in men. The g...
journal_title:Fertility and sterility
pub_type:
doi:10.1016/j.fertnstert.2019.10.004
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVE:To study the efficacy of performing two inseminations per cycle in IUI with husband's sperm compared with one insemination per cycle. DESIGN:Meta-analysis. SETTING:Randomized and prospective trials comparing two inseminations vs. one insemination per cycle in IUI with husband's sperm, retrieved by MEDLINE a...
journal_title:Fertility and sterility
pub_type: 评论,杂志文章,meta分析,评审
doi:10.1016/j.fertnstert.2003.12.035
更新日期:2004-07-01 00:00:00
abstract:OBJECTIVE:To determine the long-term survival of frozen-thawed human ovarian tissue as xenografts in severe-combined-immunodeficiency (SCID) mice. DESIGN:Animal study. SETTING:Animal and laboratory facilities at an academic center. PATIENT(S):Ovarian tissue obtained from a 27-year-old woman. INTERVENTION(S):Graftin...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(99)00548-8
更新日期:2000-03-01 00:00:00
abstract::The beta-adrenergic agonist isoproterenol was infused intravenously for 4 hours during the midluteal phase of the cycle in six normal women. A rapid increase in cardiac output occurred for the entire duration of the isoproterenol infusion and serum estrogen and progesterone levels (but not luteinizing hormone levels) ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)47712-5
更新日期:1984-03-01 00:00:00
abstract:OBJECTIVE:To determine the distribution of catalase in eutopic and ectopic endometria in patients with endometriosis or adenomyosis. DESIGN:Retrospective randomized study. SETTING:Department of obstetrics and gynecology in a university hospital. PATIENT(S):Thirty-three patients with endometriosis, 36 with adenomyosi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(02)03344-7
更新日期:2002-10-01 00:00:00
abstract::Both EST and Mayer-Rokitansky-Kuster-Hauser syndrome are uncommon disorders. This report illustrates a case of these two rare conditions occurring in the same patient. Patients with Mayer-Rokitansky-Kuster-Hauser syndrome, although not appearing to be at increased risk for germ cell tumors, unlike testicular feminizat...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)54562-2
更新日期:1991-09-01 00:00:00
abstract::Ham's F-10 medium (Gibco, Grand Island, NY) and medium T6 with and without 15% fetal calf serum (FCS) were compared for their ability to support development of murine blastocysts with the capacity to implant and produce normal fetuses when transferred to pseudopregnant females. All media supported equal rates of blast...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)59545-4
更新日期:1987-11-01 00:00:00
abstract:OBJECTIVE:To review published data and to compare pregnancy rates (PRs) after culturing human embryos with and without glucose and phosphate. DESIGN:Comparison of results from various programs. SETTING:Assisted Reproductive Technology Program. PATIENT(S):Patients were enrolled in various studies. INTERVENTION(S):Hu...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(98)00052-1
更新日期:1998-05-01 00:00:00
abstract::On the basis of studies in monkeys, the hypothesis has been proposed that vasectomy induces the formation of circulating immune complexes (CICs), which--via activation of the complement system--may result in immune injury of the endothelium and thereby exacerbate atherosclerosis; the same mechanism has been suggested ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1982-06-01 00:00:00
abstract:OBJECTIVES:To evaluate prospectively the impact of subtle tubal disorders on GIFT pregnancy rates in women with pelvic endometriosis. DESIGN:A prospective comparison of pregnancy rates in different stages of endometriosis while controlling for the presence of tubal abnormalities. INTERVENTIONS:A total of 146 patients...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)57007-1
更新日期:1994-10-01 00:00:00
abstract:OBJECTIVE:To determine the prevalence of elevated total and free T, and DHEAS, alone and in combination, in patients with polycystic ovary syndrome (PCOS). DESIGN:Cross-sectional analysis. SETTING:Tertiary care academic medical center. PATIENT(S):Seven hundred twenty patients diagnosed with PCOS according to the Nat...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.12.138
更新日期:2010-04-01 00:00:00
abstract:OBJECTIVE:To investigate whether an association exists between ovulation induction and neural tube defects (NTDs). MATERIALS AND METHODS:Risk estimations in the medical literature were identified through Medline, and validity and power were assessed. Large in vitro fertilization-embryo transfer (IVF-ET) registries rep...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/s0015-0282(16)55428-4
更新日期:1992-11-01 00:00:00
abstract:OBJECTIVE:To evaluate spontaneous apoptosis in single-cell suspensions of eutopic and ectopic endometrium from women with endometriosis and in eutopic endometrium from fertile controls without endometriosis. DESIGN:Paired specimens of eutopic and ectopic endometrial tissue from patients with endometriosis and eutopic ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(98)00073-9
更新日期:1998-06-01 00:00:00
abstract:OBJECTIVE:To evaluate the level of variance produced in a multicenter study with the use of a computer-assisted sperm morphology analyzer. DESIGN:A multicenter, prospective, blinded study. SETTING:Assisted reproduction research laboratories. PATIENT(S):Semen samples produced for assisted reproductive procedures. IN...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1016/s0015-0282(98)00421-x
更新日期:1999-01-01 00:00:00
abstract::A comparative pilot study was undertaken in three centers to determine the time of return of ovulation after the injection of the steroidal contraceptive norethisterone oenanthate (NET-OEN). The effect of the compound on the endometrium and some properties of cervical mucus were also assessed. Increases in plasma prog...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)45137-x
更新日期:1980-11-01 00:00:00
abstract:OBJECTIVE:To determine the genetic cause of primary amenorrhea. DESIGN:Case series. SETTING:Pediatric endocrinology, endocrinology, and gynecology departments of academic hospitals. PATIENT(S):Three adolescents and one young woman 46, XY patients with srd5A2 gene mutations. MAIN OUTCOME MEASURE(S):Genetic analysis ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.08.007
更新日期:2011-02-01 00:00:00
abstract:OBJECTIVE:To evaluate whether ovarian function might have an influence on the adrenal hyperandrogenism present in patients with functional ovarian hyperandrogenism. DESIGN:Controlled clinical study. SETTING:Tertiary institutional hospital. PATIENT(S):Twenty-nine hirsute women with functional ovarian hyperandrogenism...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(97)81362-3
更新日期:1997-04-01 00:00:00
abstract::Laparoscopic sterilization was performed on 50 racially distributed patients, aged 18-42 years, who were selected at random from the Outpatient Laparoscope Sterilization Clinic and offered sterilization under local anesthesia. The patients were injected with a systemic analgesic as well as a local anesthetic. One in...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)38530-2
更新日期:1971-10-01 00:00:00
abstract:OBJECTIVE:To study the effect of human plasma from different sources, namely, umbilical cord blood and adult blood platelet-rich plasma (PRP), on the regeneration of endometrial damage. DESIGN:Composition analysis, in vitro approaches, and a preclinical murine model using plasma to promote endometrial regeneration. S...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2020.07.053
更新日期:2020-08-24 00:00:00
abstract:OBJECTIVE:To describe the obstetric performance of a selected population of twin pregnancies, comparing reduced vs. nonreduced twin pregnancies after the successful completion of the 12th week. DESIGN:Prospective cohort study. SETTING:Outpatient fertility center. PATIENT(S):A cohort of 464 twin pregnancies including...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2012.09.005
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:To investigate the mutation profiles and clinical characteristics of Chinese males with isolated hypogonadotropic hypogonadism (IHH) and discover new pathogenic genes that cause IHH. DESIGN:A gene panel, including 31 known IHH genes and 52 candidate genes, was used to perform semiconductor next-generation se...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2018.04.010
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVE:To evaluate the effect of obesity on the outcome of testicular sperm extraction (TESE) and assisted reproductive technology. DESIGN:Clinical retrospective study. SETTING:Center for reproductive medicine at a tertiary-care university hospital. PATIENT(S):Nine hundred seventy patients with nonobstructive azo...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2012.10.025
更新日期:2013-02-01 00:00:00
abstract:OBJECTIVE:To create a library enriched in cDNAs from preeclamptic placentas to print onto microarrays for placental profiling of preeclampsia (PE) and high risk pregnancies. DESIGN:Prospective study. SETTING:University women's clinic and academic research laboratory. PATIENT(S):Ten patients with PE, 5 with PE and bi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.09.030
更新日期:2008-11-01 00:00:00