Abstract：
OBJECTIVE:To determine the long-term survival of frozen-thawed human ovarian tissue as xenografts in severe-combined-immunodeficiency (SCID) mice. DESIGN:Animal study. SETTING:Animal and laboratory facilities at an academic center. PATIENT(S):Ovarian tissue obtained from a 27-year-old woman. INTERVENTION(S):Grafting of frozen-thawed ovarian tissue in SCID mice for 22 weeks. MAIN OUTCOME MEASURE(S):Follicle counts and growth by morphology and PCNA staining in frozen-thawed grafts and fresh controls. RESULT(S):All three grafts were recovered intact after 22 weeks. Their stroma was devoid of necrotic cells and contained healthy follicles. The ratio of primordial-total follicles decreased significantly after grafting (0.94 +/- 0.02 to 0.87 +/- 0. 01, control vs. grafting). Compared with controls, after 22 weeks of grafting, a higher percentage of follicles had initiated growth (5.6 +/- 2.4 vs. 12.5 +/- 1.9), but there was still a significant number of primordial follicles/graft (75 +/- 6.8). Follicle stages were similar between two groups; only primordial and one-layer follicles were seen in the xenografts. In the controls, except for one two-layer follicle, the most advanced follicle was at the one-layer stage. CONCLUSION(S):Human primordial follicles survive freeze-thaw and long-term xenografting procedures and retain their capacity to initiate growth. These findings encourage future attempts for human autologous ovarian transplantation.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Oktay K,Newton H,Gosden RGdoi
10.1016/s0015-0282(99)00548-8keywords:
subject
Has Abstractpub_date
2000-03-01 00:00:00pages
599-603issue
3eissn
0015-0282issn
1556-5653pii
S0015-0282(99)00548-8journal_volume
73pub_type
杂志文章abstract：OBJECTIVE:To determine if previous treatment with clomiphene citrate intrauterine insemination (CC-IUI) affects pregnancy and high-order multiple pregnancy (HOMP) rates in subsequent hMG-IUI or FSH-IUI cycles. DESIGN:Retrospective cohort study. SETTING:Private infertility clinic. PATIENT(S):Five hundred fifty-one pa...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2003.07.031
更新日期：2004-03-01 00:00:00
abstract：OBJECTIVE:To perform a systematic review and meta-analysis of studies that compared the prevalence of anxiety symptoms in women with polycystic ovary syndrome (PCOS) and control women. DESIGN:Meta-analysis and systematic review. SETTING:University practice. PATIENT(S):Cross-sectional studies comparing PCOS subjects ...
journal_title：Fertility and sterility
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.fertnstert.2011.10.022
更新日期：2012-01-01 00:00:00
abstract：OBJECTIVE:To evaluate whether SOHLH2 intronic variation contributes to the genetic predisposition to male infertility traits, including severe oligospermia (SO) and different nonobstructive azoospermia (NOA) clinical phenotypes. DESIGN:Genetic association study. SETTING:Not applicable. PATIENT(S):Five hundred five c...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2020.02.115
更新日期：2020-08-01 00:00:00
abstract：OBJECTIVES:To examine all case reports and studies of empty follicle syndrome (EFS) that have been published since the condition was first reported, in an attempt to identify trends or common features that may expose possible risk or causative factors. DESIGN:Systematic review of all available literature obtained from...
journal_title：Fertility and sterility
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.fertnstert.2007.07.1312
更新日期：2008-09-01 00:00:00
abstract：OBJECTIVE:To quantify the in vitro effects of gonadotropins on benign, borderline, and malignant ovarian cell lines. DESIGN:In vitro cell culture. SETTING:Research laboratory. PATIENT(S):None. INTERVENTION(S):Three hormonally sensitive ovarian neoplastic cell lines were exposed to control medium, FSH (40 mIU/mL), h...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(02)03367-8
更新日期：2002-11-01 00:00:00
abstract：:In a program of human in vitro fertilization (IVF), the results of 204 attempted intracervical embryo transfers (ETs), using a variety of catheters in three trials over 18 months, have been analyzed for the ease of transfer and pregnancy rate. In nulliparous patients, transfers were more difficult than in multiparous ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)46451-4
更新日期：1982-08-01 00:00:00
abstract：OBJECTIVE:To determine whether IgG subclass patterns differed between nonpregnant women, healthy pregnant women, and pregnant women with a history of recurrent miscarriage. DESIGN:Controlled clinical study. SETTING:An academic setting. PATIENT(S):Group 1 was comprised of 10 nonpregnant women, group 2 of 10 healthy p...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(01)02857-6
更新日期：2001-11-01 00:00:00
abstract：OBJECTIVE:To review our experience with low-dose IM methotrexate for the medical management of ectopic pregnancy (EP). DESIGN:Retrospective chart review. SETTING:Magee-Womens Hospital, Pittsburgh, Pennsylvania. PATIENT(S):The first 50 women treated by the resident service in whom EP was diagnosed and treated with me...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)00284-7
更新日期：1997-10-01 00:00:00
abstract：OBJECTIVE:To study the chromosomal content of spermatozoa selected by intracytoplasmic morphologically selected sperm injection (IMSI) in cases of macrocephalic sperm head syndrome. DESIGN:Case report. SETTING:Obstetrics, gynecology, urology, and reproductive biology departments. PATIENT(S):Two infertile patients wi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.10.059
更新日期：2010-03-01 00:00:00
abstract：OBJECTIVE:Racial disparity in assisted reproductive technology (ART) outcomes has been reported but remains controversial. Reasons for the disparity are unclear, and access to care has been suggested as a causative factor. In this study, we sought to examine minority utilization of ART in the Department of Defense (DoD...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.10.028
更新日期：2006-04-01 00:00:00
abstract：OBJECTIVE:To compare pregnancy rates after fallopian tubal and uterine transfer of cryopreserved embryos. DESIGN:Prospective randomized trial with assignment to treatment groups by a random number table. SETTING:University of Iowa Hospitals and Clinics, a tertiary care academic institution. PATIENTS:Forty patients w...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(16)57429-9
更新日期：1995-03-01 00:00:00
abstract：OBJECTIVE:To assess the association between recent cigarette smoking (CS) in female and male partners and assisted reproduction technology (ART) outcomes. DESIGN:Cohort prospective study. SETTING:University ART program in Chile. PATIENT(S):One hundred sixty-six couples seeking pregnancy through ART. INTERVENTION(S)...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.09.073
更新日期：2010-01-01 00:00:00
abstract：OBJECTIVE:To present a case of Marfan syndrome and concurrent congenital obstructive azoospermia. DESIGN:Case report. SETTING:Tertiary-care academic hospital. PATIENT(S):A man with Marfan syndrome and obstructive azoospermia. INTERVENTION(S):The patient was evaluated for azoospermia that was proved to be due to con...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(01)02868-0
更新日期：2001-12-01 00:00:00
abstract：OBJECTIVE:To determine, for a woman aged >45 years, whether selected hormonal, health status, and demographic measures are related to the time to final menstrual period (FMP) from a point where 6 weeks separate the shortest and longest cycles experienced to date. DESIGN:Cohort study. SETTING:Volunteers in an academic...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(02)03231-4
更新日期：2002-08-01 00:00:00
abstract：OBJECTIVE:To determine the relative efficacy of ultrasound-guided embryo transfer and embryo transfer by clinical touch alone. DESIGN:Systematic review and meta-analysis of randomized, controlled trials comparing ultrasound-guided embryo transfer with embryo transfer by clinical touch alone. SETTING:Infertility cente...
journal_title：Fertility and sterility
pub_type： 杂志文章,meta分析,评审
doi：10.1016/s0015-0282(03)01015-x
更新日期：2003-10-01 00:00:00
abstract：OBJECTIVE:To compare two commonly used protocols (fresh vs. vitrified) used to transfer euploid blastocysts after IVF with preimplantation genetic screening. DESIGN:Randomized controlled trial. SETTING:Private assisted reproduction center. PATIENT(S):A total of 179 patients undergoing IVF treatment using preimplanta...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2016.12.022
更新日期：2017-03-01 00:00:00
abstract：OBJECTIVE:To enable serodiscordant couples to reproduce while minimizing the risk of infecting the female partner and to demonstrate the feasibility of including these couples in an insemination program. DESIGN:Prospective follow-up study. SETTING:Ambulatory IUIs. PATIENT(S):Fifty-six HIV-1-serodiscordant couples wi...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/j.fertnstert.2004.02.128
更新日期：2004-10-01 00:00:00
abstract：:A prospective randomized study was conducted in 100 women with leiomyomas in order to evaluate the effect of gestrinone, a synthetic derivative of ethynil-nor-testosterone. Patients in group A received capsules containing 2.5 mg of gestrinone three times weekly orally. Those in group B received capsules containing 5.0...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(16)60722-7
更新日期：1989-06-01 00:00:00
abstract：:DNA flow cytometry was applied to fine-needle aspiration biopsy specimens from the testes of 20 oligospermic or azoospermic men under investigation for infertility. All aspirations gave sufficient material for DNA flow cytometry, and no complications occurred after aspiration. In both groups many DNA patterns deviated...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)45014-4
更新日期：1980-10-01 00:00:00
abstract：OBJECTIVE:To identify if there are certain cutoff levels for P and or the P/E(2) ratio on the day of hCG that would be defined as detrimental for occurrence of pregnancy in women with normal ovarian reserve undergoing cleavage-stage embryo transfer (ET). Secondarily, to determine if these same cutoffs might have the sa...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2010.12.065
更新日期：2011-04-01 00:00:00
abstract：:To show that in the dysgenetic gonads of 104 Turner syndrome patients no significant difference was found regarding the expression of the genes DAX1, FOG2, GATA4, OCT4, SF1, SRY, TSPY, WT1, and STRA8 compared with controls, except for genes OCT4, SRY, and TSPY in both gonads of a patient whose chromosomal constitution...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.02.036
更新日期：2010-11-01 00:00:00
abstract：OBJECTIVE:To develop an effective method for in vitro maturation of preantral follicles isolated from mice ovarian tissue. DESIGN:Isolated preantral follicles were randomly allocated to designed experimental groups for study. SETTING:University-based research lab. PATIENT(S):Healthy, normal mice. INTERVENTION(S):Su...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(01)02977-6
更新日期：2002-02-01 00:00:00
abstract：:In this prospective, follow-up study of 102 high-risk oocyte donors in their luteal phase, we found a complete absence of ovarian hyperstimulation syndrome (no signs of hemoconcentration or ascites) after the donors were triggered with a gonadotropin releasing-hormone (GnRH) agonist. Due to its powerful preventive eff...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/j.fertnstert.2009.08.036
更新日期：2010-05-01 00:00:00
abstract：OBJECTIVE:To study the association between two single-nucleotide polymorphisms (SNPs) variants (rs2241766 and rs1501299) of the adiponectin gene (ADIPOQ) and polycystic ovary syndrome (PCOS) in PCOS family trios. DESIGN:Family-based study. SETTING:University hospital. PATIENT(S):A total of 224 unrelated PCOS proband...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.01.035
更新日期：2014-05-01 00:00:00
abstract：:This retrospective cohort study compared blastocyst transfers in 136 fresh oocyte donor cycles and 69 autologous cycles using blastocysts derived from culture of thawed bipronuclear oocytes, all with oocytes derived from patients or donors less than 35 years old. The autologous cycles and oocyte donor cycles had simil...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.07.966
更新日期：2010-01-01 00:00:00
abstract：:Bilateral hypogastric artery ligation followed by dilatation and evacuation under laparoscopic guidance was successful in the treatment of an advanced cesarean scar ectopic pregnancy. This case presents images of ultrasound, magnetic resonance imaging, and gross anatomy unique to cesarean scar pregnancy. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.12.016
更新日期：2006-05-01 00:00:00
abstract：:Both partners of 198 consecutive infertile couples were evaluated. Studies in the female included clinical evaluation of ovulation for a minimum of two menstrual cycles, investigation of tubal patency, and evaluation of sperm survival in cervical mucus. Six semen specimens were analyzed from each male. Laparoscopy, sc...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)42976-6
更新日期：1977-12-01 00:00:00
abstract：OBJECTIVE:To assess the endocrine, paracrine, and autocrine milieu in patients with endometriosis on the basis of the measurement of several cytokines in serum and follicular fluid (FF) and in vitro culture of granulosa luteal cells. DESIGN:Case-control study. SETTING:In vitro fertilization program at the Instituto V...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(98)00204-0
更新日期：1998-09-01 00:00:00
abstract：:This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.08.053
更新日期：2013-10-07 00:00:00
abstract：:The presence of ovarian cysts may compromise the success of in vitro fertilization (IVF) and gamete intrafallopian transfer (GIFT). We prospectively studied 212 consecutive ovulation induction cycles in 120 patients for IVF and/or GIFT. A baseline cyst was defined as any intraovarian cystic structure greater than or e...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)54123-5
更新日期：1991-02-01 00:00:00
