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In vitro culture and in vitro maturation of mouse preantral follicles with recombinant gonadotropins.

Abstract：

OBJECTIVE:To develop an effective method for in vitro maturation of preantral follicles isolated from mice ovarian tissue. DESIGN:Isolated preantral follicles were randomly allocated to designed experimental groups for study. SETTING:University-based research lab. PATIENT(S):Healthy, normal mice. INTERVENTION(S):Superovulation with pregnant mare serum gonadotropin and hCG. MAIN OUTCOME MEASURE(S):Morphological changes and E(2) production were assessed. RESULT(S):To obtain competent oocytes, preantral follicles must be cultured with medium containing insulin and recombinant gonadotropins (i.e., recombinant FSH and recombinant LH), with a change of medium daily. A high initial recombinant LH or recombinant FSH facilitates E(2) secretion, enhances granulosa cell outgrowth, and has earlier antral formation. However, prolonged culture in high-recombinant LH or recombinant FSH triggers early differentiation and luteinization of granulosa cells, which results in low metaphase II oocyte and blastocyst formation. CONCLUSION(S):We have developed a culture system that allows the successful maturation of preantral follicles in vitro. The matured follicles are a physiologically functional unit that not only secrete E(2) but also generate competent oocytes. In a special condition, 90% of the cultured follicles survived, 53.5% of them produced MII oocytes, and 50% of the derived MII oocytes were fertilized and reached the blastocyst stage after culture in vitro.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Liu HC,He Z,Rosenwaks Z

doi

10.1016/s0015-0282(01)02977-6

keywords:

subject

Has Abstract

pub_date

2002-02-01 00:00:00

pages

373-83

issue

2

eissn

0015-0282

issn

1556-5653

pii

S0015028201029776

journal_volume

77

pub_type

杂志文章

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