Abstract：
OBJECTIVE:To evaluate the impact of dynamic in vitro culture on initiation of early follicular growth in prepubertal mouse ovaries. DESIGN:Ovaries from 8-day-old BALB/c mice were cultured either in a dynamic system (n=28) or in a static system (n=20) for 4 days. Uncultured 8-day-old (n=9) or 12-day-old (n=17) ovaries served as baseline or in vivo controls, respectively. SETTING:Academic research center. ANIMAL(S):Newborn female BALB/c mice (n=37). INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):Histologic follicle classification and counting and assessment of follicular viability via immunofluorescent staining. RESULT(S):The percentage of secondary follicles after dynamic culture was identical to the 12-day-old in vivo control. In contrast, after static culture ovaries showed a significantly higher percentage of secondary follicles. For immunofluorescent viability assessment 6.78 follicles per ovary could be isolated after dynamic culture, whereas only 3.8 follicles per ovary could be isolated after static culture. CONCLUSION(S):Dynamic in vitro culture supports physiologic follicular growth initiation, comparable to that observed in vivo. In contrast, accelerated follicular growth was observed after static culture. These findings add additional evidence to the idea that dynamic culture might be a beneficial first step to initiate follicle growth in vitro within the context of fertility preservation.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Winkler-Crepaz K,Nederegger V,Ayuandari S,Rosenfellner D,Zervomanolakis I,Hofer S,Wildt L,Ziehr SCdoi
10.1016/j.fertnstert.2014.05.038subject
Has Abstractpub_date
2014-09-01 00:00:00pages
864-870.e2issue
3eissn
0015-0282issn
1556-5653pii
S0015-0282(14)00501-9journal_volume
102pub_type
杂志文章abstract：:Follicular development was followed by echography during 18 menstrual cycles in 10 women volunteers. Results were correlated with changes in serum hormone levels, basal body temperature curves, and cervical mucus changes. The average follicular growth as measured from the time of echographic visualization was 0.3 cm/d...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)44592-9
更新日期：1980-03-01 00:00:00
abstract：OBJECTIVE:To assess the endocrine and clinical responses to microdose GnRH agonist (GnRH-a) that was administered in the early follicular phase before controlled ovarian hyperstimulation to poor responders who were candidates for IVF-ET. DESIGN:Prospective nonrandomized trial with historical controls. SETTING:Tertiar...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)00575-x
更新日期：1998-03-01 00:00:00
abstract：OBJECTIVE:To develop an experimental model for embryos with a defect specific to hatching, with the purpose of clarifying the mammalian embryo hatching mechanism. DESIGN:A microneedle was inserted under the zona pellucida (ZP) of mouse embryos, and either 1/2 or 1/4 of the blastomeres were mechanically destroyed. The ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)55993-7
更新日期：1993-06-01 00:00:00
abstract：OBJECTIVE:To assess whether IV tramadol before outpatient hysteroscopy could reduce procedure-related pain. DESIGN:A randomized double-blind placebo controlled trial. SETTING:Outpatient Hysteroscopy Centre in the Department of Obstetrics and Gynaecology of Cagliari University. PATIENT(S):Fifty healthy, parous, women...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2006.05.072
更新日期：2007-01-01 00:00:00
abstract：OBJECTIVE:To determine the normative cut-off score that defines hirsutism among Chinese women, and the effect of age, menstrual irregularities, and polycystic ovaries on the same. DESIGN:Cross-sectional, population-based study. SETTING:A multistage systematic cluster random sampling among 16 communities from two rura...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2011.06.040
更新日期：2011-09-01 00:00:00
abstract：OBJECTIVE:To assess the association between serum adrenal cortex autoantibodies and histologically confirmed autoimmune lymphocytic oophoritis. DESIGN:Controlled, prospective. SETTING:Tertiary research center. PATIENT(S):Two hundred sixty-six women with 46,XX spontaneous premature ovarian failure. INTERVENTION(S):O...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.04.060
更新日期：2005-10-01 00:00:00
abstract：:The effects of various doses of indomethacin and ketoprofen as compared with placebo were examined in 100 oligospermic patients who participated in this study. It was found that the treatment increased sperm count, sperm motility, and fertilizing capacity. The radioimmunoassay examination showed an increase in plasma ...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)48081-7
更新日期：1984-09-01 00:00:00
abstract：OBJECTIVE:To compare ovarian response to hyperstimulation during IVF between patients who did and did not undergo salpingectomy. DESIGN:Meta-analysis. SETTING:University-affiliated teaching hospital. PATIENT(S):Patients undergoing IVF who did and did not undergo salpingectomy. INTERVENTION(S):None. MAIN OUTCOME ME...
journal_title：Fertility and sterility
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.fertnstert.2016.03.053
更新日期：2016-08-01 00:00:00
abstract：OBJECTIVE:To report the occurrence of spontaneous regression of three endometrial polyps detected by hysteroscopy. DESIGN:Case series. SETTING:A uterine imaging unit in an academic medical center. PATIENT(S):Three patients diagnosed as having an endometrial polyp of 5-8 mm on hysteroscopy. INTERVENTION(S):Patient d...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.04.054
更新日期：2009-08-01 00:00:00
abstract：:Insulin-like growth factor I (IGF-I) has been proposed to be an autocrine/paracrine factor involved in granulosa cell proliferation and differentiation. The present study focuses on a possible mitogenic effect of IGF-I in human granulosa cells. Insulin-like growth factor I (1 to 10 ng/mL) significantly stimulated 3H-t...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:A retrospective review of 237 initial, fresh nondonor IVF cycles in which all embryos generated during the cycle were transferred on either day 2 (n = 109) or day 3 (n = 128) were evaluated with regards to reproductive outcomes. Patients who underwent a day 2 ET had similar conception (18% vs. 16%; relative risk [RR],...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.10.010
更新日期：2011-03-01 00:00:00
abstract：OBJECTIVE:To determine the incidence and type of mixoploidy in human blastocysts produced in vitro. DESIGN:A laboratory study of spare blastocysts from an IVF program. SETTING:University hospital laboratory. PATIENT(S):Thirty-nine couples undergoing IVF or intracytoplasmic sperm injection. INTERVENTION(S):A total o...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(02)04393-5
更新日期：2002-12-01 00:00:00
abstract：:The effects of various concentrations of rosiglitazone on in vitro rat embryo growth and morphology during the critical period of organogenesis was evaluated. We found that rosiglitazone has no significant effects on growth and morphology of in vitro early embryo development, even at very high concentrations. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.12.069
更新日期：2006-08-01 00:00:00
abstract：:A study was undertaken comparing the outcomes of 30 women with infertility due to untreated severe (grade IV) pelvic endometriosis with a comparable series of 28 women whose infertility was caused solely by irreversible tubal disease. There were no significant differences in either the follicular phase or luteal phase...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60078-x
更新日期：1988-08-01 00:00:00
abstract：:In two patients with apparent PTO, as diagnosed by HSG and laparoscopy, tubal patency was restored by hysteroscopic cannulation of the tubal ostia, followed by direct lavage. Tubal resection and reanastomosis/reimplantation may not be necessary for all patients with apparent PTO. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1987-09-01 00:00:00
abstract：OBJECTIVE:To evaluate the size of ovarian damage caused by ovarian drilling in polycystic ovary syndrome, the amount of inflicted damage was assessed for the most frequently used ovarian drilling techniques. DESIGN:Experimental prospective design. SETTING:University clinic. PATIENT(S):Six fresh bovine ovaries per te...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.10.046
更新日期：2010-02-01 00:00:00
abstract：:Three groups of dogs underwent testicular ischemia for a 3-hour period. Group I (10 animals) was not subsequently perfused. Group II (10 animals) underwent subsequent perfusion by a new in vivo reversible method via the internal spermatic artery with heparinized saline. Group III (5 animals) underwent perfusion with S...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)42924-9
更新日期：1977-11-01 00:00:00
abstract：:Constant infusion of gonadotropin-releasing hormone agonist (GnRH-A) in the rhesus monkey leads to far greater suppression of spermatogenesis than its intermittent administration. To assess whether administration of GnRH-A by constant subcutaneous infusion will also lead to greater inhibition of gonadal function, we a...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)48684-x
更新日期：1985-07-01 00:00:00
abstract：:22 physician-providers who serve natural family planning (NFP) programs, mostly in the private sector, gathered to formulate a standard terminology for the field. The Billings and sympto-thermal methods are the 2 methods taught by this group. The sypto-thermal method helps a woman predict ovulation by detecting chan...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)46409-5
更新日期：1982-07-01 00:00:00
abstract：:Two hundred twenty consecutive in vitro fertilization (IVF) conception cycles were studied prospectively in order to examine the predictive value of serum human chorionic gonadotropin beta-subunit (beta-hCG), estradiol (E2), and progesterone (P) in predicting pregnancy outcome between 2 and 4 weeks after oocyte collec...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60495-8
更新日期：1989-02-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to assess which of the basal ovarian reserve markers provides the best reflection of the changes occurring in ovarian function over time (i.e., reproductive aging). DESIGN:Prospective longitudinal study. SETTING:Healthy volunteers in an academic research center. PATIENT(S):Eighty-...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.11.029
更新日期：2005-04-01 00:00:00
abstract：OBJECTIVE:To describe ovulation induction and pregnancy outcome in a unique model of genetically determined combined pituitary hormone deficiency (CPHD), with respect to the necessity for GH substitution therapy. DESIGN:Case report. SETTING:Academic units. PATIENT(S):Two patients with childhood onset of CPHD (GH, PR...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.03.026
更新日期：2004-08-01 00:00:00
abstract：OBJECTIVE:Potential reparation of sperm DNA fragmentation in the oocyte may disturb any relationship between DNA-damaged sperm and the implantation ability of resulting embryos. To rule out this factor, we analyzed the consequences of sperm DNA fragmentation on IVF-ET outcome in women with healthy ovarian function. DE...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/j.fertnstert.2007.02.022
更新日期：2008-01-01 00:00:00
abstract：:One hundred infertile patients with laparoscopically confirmed ovarian endometriosis were treated with either intranasal (IN) Buserelin (Hoechst, AG, Frankfurt am Main, West Germany) (300 micrograms three times a day) or subcutaneous (SC) Buserelin implant (6.6 mg Buserelin). Serum estradiol was suppressed in the meno...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)60783-5
更新日期：1989-07-01 00:00:00
abstract：:Chronic treatment with highly active analogs of luteinizing hormone (LH)-releasing hormone (LH-RH) induces "paradoxical" antifertility effects. Intact male rats, a reduction in testosterone production is observed after the administration of [D-Ser(But)6]-LH-RH(1-9)ethylamide (burserelin, Hoe 766), 50 ng/day subcutaneo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)43461-8
更新日期：1978-08-01 00:00:00
abstract：OBJECTIVE:To demonstrate laparoscopic surgery for a patient with unicornuate uterus and a large hematometra in the noncommunicating uterine horn. DESIGN:Narrated video featuring the diagnostic tests and surgical management. SETTING:Academic tertiary hospital. PATIENT(S):A 13-year-old woman, gravida 0 with menarche a...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2020.05.042
更新日期：2020-11-01 00:00:00
abstract：OBJECTIVE:To evaluate the effectiveness of sublingual misoprostol for cervical ripening before diagnostic hysteroscopy in premenopausal women. DESIGN:Placebo-controlled, double-blind, randomized trial. SETTING:University hospital. PATIENT(S):Fifty-two women with an indication for diagnostic hysteroscopy. INTERVENTI...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2009.01.073
更新日期：2010-05-01 00:00:00
abstract：OBJECTIVE:We evaluated the association between subfertility and low birth weight infants. DESIGN:Women in this analysis participated in a cross-sectional investigation of maternal risk factors for adverse pregnancy outcomes. SETTING:Medical and obstetric history and life style information were obtained from women who...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)54597-x
更新日期：1991-10-01 00:00:00
abstract：OBJECTIVE:To assess the relationship between history of surgery for endometriosis and adverse obstetrical outcomes. DESIGN:Retrospective study using prospectively recorded data. SETTING:Referral center. PATIENT(S):Total of 569 women with history of surgery for endometriosis, postoperative conception, and pregnancy e...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2019.12.037
更新日期：2020-05-01 00:00:00
abstract：OBJECTIVE:To assess the ability of a GnRH antagonist (Cetrorelix, Asta Medica AG, Frankfurt, Germany) to prevent premature LH surges in an IVF-ET program using a simple protocol with one or two administrations. DESIGN:Controlled ovarian hyperstimulation was carried out in 17 women with three ampules a day of hMG, star...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)56933-7
更新日期：1994-09-01 00:00:00
