Effect of chilling on the organization of tubulin and chromosomes in rhesus monkey oocytes.

Abstract:

OBJECTIVE:To determine the effects of chilling on the organization and distribution of tubulin and chromosomes in rhesus monkey oocytes. DESIGN:Comparative laboratory study. SETTING:Academic research laboratory. ANIMAL(S):Eight adult female rhesus monkeys (Macaca mulatta) aged 6-16 years. INTERVENTION(S):A total of 171 oocytes retrieved from eight rhesus monkeys were separated into nine groups. One group of control oocytes was held at 37 degrees C during the experiment. Four groups of oocytes were rapidly cooled to 0 degrees C and held for 1, 5, 10, or 30 minutes and then fixed and stained. Four other groups of oocytes were cooled to 0 degrees C, held for 1, 5, 10, or 30 minutes, warmed and incubated at 37 degrees C for 60 minutes, and then fixed and stained. MAIN OUTCOME MEASURE(S):Organization of cytoskeleton and chromosomes. RESULT(S):Exposure of rhesus oocytes to 0 degrees C for only 1 minute resulted in complete depolymerization of tubulin. Incubation of chilled oocytes at 37 degrees C for 60 minutes caused partial restoration of tubulin, although most oocytes exhibited abnormal alignment of chromosomes and disorganized meiotic spindles. CONCLUSION(S):We conclude that rhesus monkey oocytes are extremely sensitive to chilling injury. Their successful cryopreservation may require rapid cooling to outpace this injury.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Songsasen N,Yu IJ,Ratterree MS,VandeVoort CA,Leibo SP

doi

10.1016/s0015-0282(01)03240-x

keywords:

subject

Has Abstract

pub_date

2002-04-01 00:00:00

pages

818-25

issue

4

eissn

0015-0282

issn

1556-5653

pii

S001502820103240X

journal_volume

77

pub_type

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